{"id":"https://openalex.org/W79181394","doi":null,"title":"Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris","display_name":"Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris","publication_year":2003,"publication_date":"2003-01-01","ids":{"openalex":"https://openalex.org/W79181394","mag":"79181394"},"language":"en","primary_location":{"id":"pmh:http://www.rian.ie/54730/","doi":null,"is_oa":true,"landing_page_url":"http://doras.dcu.ie/17247/","pdf_url":"http://doras.dcu.ie/17247/","source":{"id":"https://openalex.org/S4306400033","display_name":"Arrow@dit (Dublin Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210144925","host_organization_name":"Technological University Dublin","host_organization_lineage":["https://openalex.org/I4210144925"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Carty, Charlene (2003) Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris. Master of Science thesis, Dublin City University.","raw_type":"Other"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://doras.dcu.ie/17247/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087074459","display_name":"Charlene Carty","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Charlene Carty","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5087074459"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10695","display_name":"Parasites and Host Interactions","score":0.9663000106811523,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10695","display_name":"Parasites and Host Interactions","score":0.9663000106811523,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.01360000018030405,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/schistosoma-mansoni","display_name":"Schistosoma mansoni","score":0.9649999737739563},{"id":"https://openalex.org/keywords/cathepsin-c","display_name":"cathepsin C","score":0.9909999966621399},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.8510000109672546},{"id":"https://openalex.org/keywords/pichia-pastoris-expression-system","display_name":"Pichia pastoris expression","score":0.9110000133514404},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8460000157356262},{"id":"https://openalex.org/keywords/proenzyme","display_name":"proenzyme","score":0.5419999957084656},{"id":"https://openalex.org/keywords/proteolytic-activity","display_name":"proteolytic activity","score":0.703000009059906}],"concepts":[{"id":"https://openalex.org/C2781372317","wikidata":"https://www.wikidata.org/wiki/Q148697","display_name":"Pichia pastoris","level":4,"score":0.8240187168121338},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.7940431833267212},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7381762862205505},{"id":"https://openalex.org/C189482833","wikidata":"https://www.wikidata.org/wiki/Q869146","display_name":"cDNA library","level":4,"score":0.6433813571929932},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5986714959144592},{"id":"https://openalex.org/C2779703480","wikidata":"https://www.wikidata.org/wiki/Q125153","display_name":"Pichia","level":5,"score":0.5632447004318237},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.5290338397026062},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.40863579511642456},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2500269412994385},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2223193347454071}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:http://www.rian.ie/54730/","doi":null,"is_oa":true,"landing_page_url":"http://doras.dcu.ie/17247/","pdf_url":"http://doras.dcu.ie/17247/","source":{"id":"https://openalex.org/S4306400033","display_name":"Arrow@dit (Dublin Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210144925","host_organization_name":"Technological University Dublin","host_organization_lineage":["https://openalex.org/I4210144925"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Carty, Charlene (2003) Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris. Master of Science thesis, Dublin City University.","raw_type":"Other"},{"id":"pmh:oai:doras.dcu.ie:17247","doi":null,"is_oa":true,"landing_page_url":"https://doras.dcu.ie/17247/1/charlene_carty_20120705093351.pdf","pdf_url":"https://doras.dcu.ie/17247/1/charlene_carty_20120705093351.pdf","source":{"id":"https://openalex.org/S4306401511","display_name":"Dublin City University Open Access Institutional Repository (Dublin City University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I42934936","host_organization_name":"Dublin City University","host_organization_lineage":["https://openalex.org/I42934936"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:79181394","doi":null,"is_oa":false,"landing_page_url":"http://doras.dcu.ie/17247/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:http://www.rian.ie/54730/","doi":null,"is_oa":true,"landing_page_url":"http://doras.dcu.ie/17247/","pdf_url":"http://doras.dcu.ie/17247/","source":{"id":"https://openalex.org/S4306400033","display_name":"Arrow@dit (Dublin Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210144925","host_organization_name":"Technological University Dublin","host_organization_lineage":["https://openalex.org/I4210144925"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Carty, Charlene (2003) Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris. Master of Science thesis, Dublin City University.","raw_type":"Other"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W79181394.pdf","grobid_xml":"https://content.openalex.org/works/W79181394.grobid-xml"},"referenced_works_count":120,"referenced_works":["https://openalex.org/W152127350","https://openalex.org/W170965345","https://openalex.org/W262405946","https://openalex.org/W1500472831","https://openalex.org/W1512472858","https://openalex.org/W1550528885","https://openalex.org/W1551654836","https://openalex.org/W1564179195","https://openalex.org/W1567730256","https://openalex.org/W1588193324","https://openalex.org/W1679023432","https://openalex.org/W1776073229","https://openalex.org/W1812339273","https://openalex.org/W1878275597","https://openalex.org/W1911906532","https://openalex.org/W1963587605","https://openalex.org/W1964812124","https://openalex.org/W1965020882","https://openalex.org/W1966999658","https://openalex.org/W1969696437","https://openalex.org/W1970318535","https://openalex.org/W1970472661","https://openalex.org/W1972584860","https://openalex.org/W1974699206","https://openalex.org/W1975351631","https://openalex.org/W1977260572","https://openalex.org/W1979629723","https://openalex.org/W1979988142","https://openalex.org/W1980692180","https://openalex.org/W1981915215","https://openalex.org/W1985418324","https://openalex.org/W1986216442","https://openalex.org/W1988599801","https://openalex.org/W1990861050","https://openalex.org/W1991032754","https://openalex.org/W1992707998","https://openalex.org/W1997702624","https://openalex.org/W1998361179","https://openalex.org/W1998923348","https://openalex.org/W2000266693","https://openalex.org/W2001570021","https://openalex.org/W2004587190","https://openalex.org/W2005266650","https://openalex.org/W2007486993","https://openalex.org/W2009972285","https://openalex.org/W2014994279","https://openalex.org/W2015020062","https://openalex.org/W2018457584","https://openalex.org/W2021134612","https://openalex.org/W2023853843","https://openalex.org/W2025037117","https://openalex.org/W2026868764","https://openalex.org/W2027070041","https://openalex.org/W2028235694","https://openalex.org/W2028724191","https://openalex.org/W2029671854","https://openalex.org/W2034309871","https://openalex.org/W2035163490","https://openalex.org/W2040059111","https://openalex.org/W2041869283","https://openalex.org/W2042851827","https://openalex.org/W2043045484","https://openalex.org/W2045631896","https://openalex.org/W2045828320","https://openalex.org/W2047392062","https://openalex.org/W2047798770","https://openalex.org/W2047935476","https://openalex.org/W2049352187","https://openalex.org/W2054054776","https://openalex.org/W2055043387","https://openalex.org/W2058268622","https://openalex.org/W2058468601","https://openalex.org/W2059451237","https://openalex.org/W2060450994","https://openalex.org/W2061278857","https://openalex.org/W2061331839","https://openalex.org/W2061734833","https://openalex.org/W2062602533","https://openalex.org/W2063917927","https://openalex.org/W2066779845","https://openalex.org/W2070018377","https://openalex.org/W2070562519","https://openalex.org/W2073684122","https://openalex.org/W2074295581","https://openalex.org/W2074602088","https://openalex.org/W2075409231","https://openalex.org/W2084172998","https://openalex.org/W2084855662","https://openalex.org/W2089767571","https://openalex.org/W2091556637","https://openalex.org/W2092033006","https://openalex.org/W2092603929","https://openalex.org/W2092997121","https://openalex.org/W2093915148","https://openalex.org/W2094349654","https://openalex.org/W2097125253","https://openalex.org/W2102501687","https://openalex.org/W2106220465","https://openalex.org/W2108786034","https://openalex.org/W2120357935","https://openalex.org/W2123339897","https://openalex.org/W2124157676","https://openalex.org/W2130162256","https://openalex.org/W2130225747","https://openalex.org/W2135298082","https://openalex.org/W2141492848","https://openalex.org/W2142535418","https://openalex.org/W2147923184","https://openalex.org/W2149034004","https://openalex.org/W2161368535","https://openalex.org/W2164429509","https://openalex.org/W2164663952","https://openalex.org/W2171777269","https://openalex.org/W2171795759","https://openalex.org/W2187144093","https://openalex.org/W2312764775","https://openalex.org/W2335362735","https://openalex.org/W2344863420","https://openalex.org/W2403799334","https://openalex.org/W2952540019"],"related_works":["https://openalex.org/W94821548","https://openalex.org/W7135909464","https://openalex.org/W1985728382","https://openalex.org/W2130020291","https://openalex.org/W7100100222","https://openalex.org/W2418603062","https://openalex.org/W3199387563","https://openalex.org/W1998408142","https://openalex.org/W2768057790","https://openalex.org/W1776073229"],"abstract_inverted_index":{"The":[0,33,259],"cDNA":[1,18,37,152],"encoding":[2],"the":[3,9,41,50,54,61,71,78,91,97,115,126,138,143,149,158,208,211,216,220,230,235,278],"proenzyme":[4],"of":[5,53,60,114,121,140,161,168,174,190,210,219,252,275,287],"cathepsin":[6,35,74,116,150,237,255,307],"C":[7,36,75,117,128,151,238,256],"from":[8,16,90],"parasitic":[10],"helminth":[11],"Schistosoma":[12],"mansoni":[13,73,270],"was":[14,38,87,94,130,164,185,232,257,261,289],"amplified":[15,89],"a":[17,67,111,171,200],"library":[19,92],"using":[20],"5\u2019":[21],"and":[22,30,93,100,194,203,282],"3\u2019":[23],"specific":[24],"primers":[25],"containing":[26],"sites":[27],"for":[28,180],"SnaBI":[29],"Avrll":[31],"digestion.":[32],"full-length":[34],"cloned":[39,95],"into":[40,96,157],"Pichia":[42],"pastoris":[43,123],"expression":[44,163],"vector,":[45],"pPIC9K,":[46],"in":[47,77,244],"frame":[48],"with":[49,132],"initiation":[51],"codon":[52],"yeast":[55,159],"a-factor":[56],"signal":[57],"sequence.":[58],"Sequencing":[59],"1.3":[62],"kb":[63],"cDNA-vector":[64],"construct":[65,129],"revealed":[66,147],"94.5%":[68],"identity":[69],"to":[70,102,109,119,142,170,215,227,248,263,267,301,304],"S.":[72,269],"sequence":[76],"public":[79],"database":[80],"at":[81,291,296],"amino":[82],"acid":[83],"level.\\n\\nA":[84],"smaller":[85],"fragment":[86,107],"also":[88,295],"pGEM":[98],"vector":[99],"sequenced":[101],"characterise":[103],"its":[104,268],"identity.":[105],"This":[106],"proved":[108,242,300],"be":[110,302],"truncated":[112],"version":[113],"cDNA.\\n\\nPrior":[118],"transformation":[120],"P.":[122],"strain":[124],"GS115,":[125],"pPIC9K-cathepsin":[127],"linearised":[131],"Bglll.":[133],"Transformants":[134],"were":[135,197,225],"selected":[136],"on":[137],"basis":[139],"resistance":[141],"antibiotic":[144],"G418.":[145],"PCR":[146],"that":[148],"had":[153],"been":[154],"successfully":[155],"integrated":[156],"genome":[160],"transformants.\\n\\nProtein":[162],"induced":[165],"by":[166,187,277],"addition":[167],"methanol":[169],"final":[172],"concentration":[173],"1.0-1.5%,":[175],"once":[176],"or":[177],"twice":[178],"daily":[179],"six":[181],"days.":[182],"Recombinant":[183],"protein":[184],"detected":[186],"SDS-PAGE.":[188],"Proteins":[189],"58,":[191],"55,":[192],"47":[193],"25":[195],"kDa":[196],"produced":[198],"but":[199,294],"western":[201],"blot":[202],"ELISA":[204],"did":[205],"not":[206],"detect":[207],"presence":[209],"hexahistidine":[212],"tag":[213],"fused":[214],"COOH":[217],"terminus":[218],"recombinant":[221],"enzyme.":[222],"Protease":[223],"assays":[224],"performed":[226],"determine":[228],"if":[229],"enzyme":[231,260],"active":[233],"against":[234],"fluorogenic":[236],"substrate":[239],"H-Gly-Arg-NHMec.":[240],"Activity":[241],"low":[243],"all":[245],"instances":[246],"relative":[247],"positive":[249],"controls.\\n\\nBiochemical":[250],"characterisation":[251],"Fasciola":[253],"hepatica":[254,306],"undertaken.":[258],"demonstrated":[262],"exhibit":[264],"similar":[265],"properties":[266],"counterpart":[271],"such":[272],"as":[273],"enhancement":[274],"activity":[276,288],"reducing":[279],"agent":[280],"DTT":[281],"halide":[283],"ions.":[284],"A":[285],"peak":[286],"seen":[290],"pH":[292,297],"5.5":[293],"8.5,":[298],"which":[299],"unique":[303],"F":[305],"C.":[308]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2016-06-24T00:00:00"}
