{"id":"https://openalex.org/W7812954","doi":null,"title":"DISSECTING THE MECHANISM OF NLRP3 INFLAMMASOME ACTIVATION IN INDIVIDUAL CELLS: THE ROLE OF REACTIVE OXYGEN SPECIES AND ORGANELLE DAMAGE","display_name":"DISSECTING THE MECHANISM OF NLRP3 INFLAMMASOME ACTIVATION IN INDIVIDUAL CELLS: THE ROLE OF REACTIVE OXYGEN SPECIES AND ORGANELLE DAMAGE","publication_year":2013,"publication_date":"2013-11-22","ids":{"openalex":"https://openalex.org/W7812954","mag":"7812954"},"language":"en","primary_location":{"id":"pmh:oai:d-scholarship.pitt.edu:20071","doi":null,"is_oa":false,"landing_page_url":"https://d-scholarship.pitt.edu/20071/1/heid_thesis_document.pdf","pdf_url":"https://d-scholarship.pitt.edu/20071/1/heid_thesis_document.pdf","source":{"id":"https://openalex.org/S4306402372","display_name":"D-Scholarship@Pitt (University of Pittsburgh)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I170201317","host_organization_name":"University of Pittsburgh","host_organization_lineage":["https://openalex.org/I170201317"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"PeerReviewed"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079217545","display_name":"Michelle E. Heid","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Michelle E. Heid","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5079217545"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11078","display_name":"Inflammasome and immune disorders","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11078","display_name":"Inflammasome and immune disorders","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10371","display_name":"Immune Response and Inflammation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/inflammasome-activation","display_name":"inflammasome activation","score":0.8949999809265137},{"id":"https://openalex.org/keywords/reactive-oxygen-species","display_name":"reactive oxygen species","score":0.8519999980926514},{"id":"https://openalex.org/keywords/mitochondrial-ros","display_name":"mitochondrial reactive oxygen species","score":0.8569999933242798},{"id":"https://openalex.org/keywords/lysosomal-membrane-permeabilization","display_name":"lysosomal membrane permeabilization","score":0.9800000190734863},{"id":"https://openalex.org/keywords/mitochondrial-membrane-permeabilization","display_name":"mitochondrial membrane permeabilization","score":0.9959999918937683},{"id":"https://openalex.org/keywords/il-1beta-secretion","display_name":"IL-1\u03b2 secretion","score":0.8949999809265137},{"id":"https://openalex.org/keywords/nigericin","display_name":"nigericin","score":0.8920000195503235},{"id":"https://openalex.org/keywords/atp","display_name":"ATP","score":0.8960000276565552},{"id":"https://openalex.org/keywords/p2x7-receptor","display_name":"P2X7 receptor","score":0.9279999732971191},{"id":"https://openalex.org/keywords/p2x4-receptor","display_name":"P2X4 receptor","score":0.9950000047683716},{"id":"https://openalex.org/keywords/organelle-crosstalk","display_name":"organelle crosstalk","score":0.8610000014305115},{"id":"https://openalex.org/keywords/bone-marrow-derived-macrophages","display_name":"bone marrow-derived macrophages","score":0.8109999895095825},{"id":"https://openalex.org/keywords/dendritic-cells","display_name":"dendritic cells","score":0.972000002861023},{"id":"https://openalex.org/keywords/caspase-1","display_name":"caspase-1","score":0.7139999866485596},{"id":"https://openalex.org/keywords/asc","display_name":"ASC","score":0.6570000052452087},{"id":"https://openalex.org/keywords/live-cell-imaging","display_name":"live-cell imaging","score":0.4429999887943268},{"id":"https://openalex.org/keywords/single-cell-analysis","display_name":"single-cell analysis","score":0.6100000143051147}],"concepts":[{"id":"https://openalex.org/C2777330628","wikidata":"https://www.wikidata.org/wiki/Q7033077","display_name":"Nigericin","level":3,"score":0.9809762239456177},{"id":"https://openalex.org/C2777209026","wikidata":"https://www.wikidata.org/wiki/Q412405","display_name":"Inflammasome","level":3,"score":0.9645262360572815},{"id":"https://openalex.org/C187831198","wikidata":"https://www.wikidata.org/wiki/Q18034187","display_name":"AIM2","level":4,"score":0.7376181483268738},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.7365490198135376},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.6217750906944275},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5354893803596497},{"id":"https://openalex.org/C48349386","wikidata":"https://www.wikidata.org/wiki/Q424361","display_name":"Reactive oxygen species","level":2,"score":0.4643709063529968},{"id":"https://openalex.org/C139964883","wikidata":"https://www.wikidata.org/wiki/Q14901964","display_name":"Caspase 1","level":4,"score":0.4500404894351959},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.366153359413147},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.35402315855026245},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.12844684720039368},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.08029875159263611}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:d-scholarship.pitt.edu:20071","doi":null,"is_oa":false,"landing_page_url":"https://d-scholarship.pitt.edu/20071/1/heid_thesis_document.pdf","pdf_url":"https://d-scholarship.pitt.edu/20071/1/heid_thesis_document.pdf","source":{"id":"https://openalex.org/S4306402372","display_name":"D-Scholarship@Pitt (University of Pittsburgh)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I170201317","host_organization_name":"University of Pittsburgh","host_organization_lineage":["https://openalex.org/I170201317"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"PeerReviewed"},{"id":"mag:7812954","doi":null,"is_oa":false,"landing_page_url":"http://d-scholarship.pitt.edu/20071/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":180,"referenced_works":["https://openalex.org/W121387658","https://openalex.org/W133430086","https://openalex.org/W146312067","https://openalex.org/W1483162601","https://openalex.org/W1529674879","https://openalex.org/W1536268032","https://openalex.org/W1539730987","https://openalex.org/W1570116817","https://openalex.org/W1680053970","https://openalex.org/W1743744546","https://openalex.org/W1756750202","https://openalex.org/W1891826181","https://openalex.org/W1966382115","https://openalex.org/W1967535998","https://openalex.org/W1968182403","https://openalex.org/W1968284620","https://openalex.org/W1968449102","https://openalex.org/W1968564077","https://openalex.org/W1970026235","https://openalex.org/W1970154782","https://openalex.org/W1970436087","https://openalex.org/W1971915082","https://openalex.org/W1973645470","https://openalex.org/W1975032025","https://openalex.org/W1975145629","https://openalex.org/W1975752601","https://openalex.org/W1978007043","https://openalex.org/W1981120301","https://openalex.org/W1981627206","https://openalex.org/W1983736296","https://openalex.org/W1984520240","https://openalex.org/W1985538633","https://openalex.org/W1985970823","https://openalex.org/W1986568749","https://openalex.org/W1986843662","https://openalex.org/W1987535382","https://openalex.org/W1988197452","https://openalex.org/W1988654439","https://openalex.org/W1989026904","https://openalex.org/W1989511723","https://openalex.org/W1989551786","https://openalex.org/W1994473516","https://openalex.org/W1995586746","https://openalex.org/W1997136952","https://openalex.org/W1997982516","https://openalex.org/W1999227948","https://openalex.org/W2001946532","https://openalex.org/W2006790257","https://openalex.org/W2007747853","https://openalex.org/W2008868638","https://openalex.org/W2008918642","https://openalex.org/W2009004909","https://openalex.org/W2009435405","https://openalex.org/W2009800174","https://openalex.org/W2011075236","https://openalex.org/W2014697045","https://openalex.org/W2015449243","https://openalex.org/W2016293419","https://openalex.org/W2018173306","https://openalex.org/W2018563185","https://openalex.org/W2018607502","https://openalex.org/W2018969070","https://openalex.org/W2020908160","https://openalex.org/W2023368253","https://openalex.org/W2023567224","https://openalex.org/W2024730637","https://openalex.org/W2028545985","https://openalex.org/W2035664000","https://openalex.org/W2035847101","https://openalex.org/W2036906474","https://openalex.org/W2037307064","https://openalex.org/W2037600884","https://openalex.org/W2038096100","https://openalex.org/W2038481472","https://openalex.org/W2042767581","https://openalex.org/W2042816890","https://openalex.org/W2044247344","https://openalex.org/W2047177644","https://openalex.org/W2047820343","https://openalex.org/W2048032544","https://openalex.org/W2048446996","https://openalex.org/W2051930121","https://openalex.org/W2052874989","https://openalex.org/W2053352311","https://openalex.org/W2054571187","https://openalex.org/W2054784071","https://openalex.org/W2055654995","https://openalex.org/W2055793916","https://openalex.org/W2058599679","https://openalex.org/W2058760545","https://openalex.org/W2059760435","https://openalex.org/W2064741210","https://openalex.org/W2064833530","https://openalex.org/W2068048197","https://openalex.org/W2068189492","https://openalex.org/W2068381032","https://openalex.org/W2068538439","https://openalex.org/W2070728859","https://openalex.org/W2071766782","https://openalex.org/W2072686851","https://openalex.org/W2073788301","https://openalex.org/W2075611712","https://openalex.org/W2076062022","https://openalex.org/W2076063079","https://openalex.org/W2078137602","https://openalex.org/W2078156178","https://openalex.org/W2079310705","https://openalex.org/W2079781432","https://openalex.org/W2079882002","https://openalex.org/W2080878773","https://openalex.org/W2082315075","https://openalex.org/W2083859580","https://openalex.org/W2084383502","https://openalex.org/W2087616650","https://openalex.org/W2089003246","https://openalex.org/W2089250915","https://openalex.org/W2091501729","https://openalex.org/W2092843498","https://openalex.org/W2093631337","https://openalex.org/W2094241924","https://openalex.org/W2097391811","https://openalex.org/W2097714951","https://openalex.org/W2098097777","https://openalex.org/W2100358971","https://openalex.org/W2101412709","https://openalex.org/W2102217682","https://openalex.org/W2103200355","https://openalex.org/W2103358704","https://openalex.org/W2105149937","https://openalex.org/W2105430590","https://openalex.org/W2107182434","https://openalex.org/W2108723776","https://openalex.org/W2109547698","https://openalex.org/W2113010696","https://openalex.org/W2113200138","https://openalex.org/W2113485094","https://openalex.org/W2114769428","https://openalex.org/W2118800225","https://openalex.org/W2119098586","https://openalex.org/W2122287804","https://openalex.org/W2122708413","https://openalex.org/W2123640394","https://openalex.org/W2125889488","https://openalex.org/W2126384811","https://openalex.org/W2127237840","https://openalex.org/W2128627731","https://openalex.org/W2129114362","https://openalex.org/W2129319296","https://openalex.org/W2133557129","https://openalex.org/W2134219545","https://openalex.org/W2134735862","https://openalex.org/W2137500094","https://openalex.org/W2137590150","https://openalex.org/W2138838898","https://openalex.org/W2138996282","https://openalex.org/W2142821165","https://openalex.org/W2145235233","https://openalex.org/W2146049932","https://openalex.org/W2147841213","https://openalex.org/W2147978858","https://openalex.org/W2148299820","https://openalex.org/W2149605526","https://openalex.org/W2153842328","https://openalex.org/W2153875468","https://openalex.org/W2154690635","https://openalex.org/W2155138367","https://openalex.org/W2155367636","https://openalex.org/W2155643696","https://openalex.org/W2155738620","https://openalex.org/W2157490013","https://openalex.org/W2158882241","https://openalex.org/W2161114503","https://openalex.org/W2163647699","https://openalex.org/W2165655956","https://openalex.org/W2166865493","https://openalex.org/W2167121345","https://openalex.org/W2167344953","https://openalex.org/W2169856031","https://openalex.org/W2171309698","https://openalex.org/W2171576765"],"related_works":["https://openalex.org/W2155738620","https://openalex.org/W4313353950","https://openalex.org/W2170064977","https://openalex.org/W2048032544","https://openalex.org/W2024060915","https://openalex.org/W2413082478","https://openalex.org/W3200394304","https://openalex.org/W1557442887","https://openalex.org/W2794560778","https://openalex.org/W2907088267"],"abstract_inverted_index":{"Inflammation":[0],"underlies":[1],"the":[2,14,25,32,76,84,121,177,242,277,289,330,346],"pathology":[3],"of":[4,16,27,41,63,86,125,128,146,156,162,180,192,204,222,229,241,247,279,323,333],"numerous":[5],"diseases.":[6,319],"It":[7],"can":[8],"be":[9],"initiated":[10],"by":[11,59],"macrophages":[12,106],"through":[13],"secretion":[15,124,257,335],"pro-inflammatory":[17,331],"cytokines,":[18],"such":[19,48,70],"as":[20,49,71,194,309],"IL-18":[21],"and":[22,52,75,93,120,123,154,219,275,291,345],"IL-1\u03b2,":[23],"following":[24],"activation":[26,89,102,170,207,240,274],"a":[28,36,45,60,151,172,189,201,226,264,294,310],"molecular":[29],"complex":[30,39],"called":[31],"inflammasome.":[33,244],"Inflammasomes":[34],"are":[35],"protein":[37],"scaffolding":[38],"consisting":[40],"three":[42],"known":[43],"components:":[44],"sensory":[46],"NLR,":[47],"NLRP3,":[50],"ASC,":[51],"caspase-1.":[53],"The":[54],"NLRP3":[55,87,100,168,205,230,243,272,298,316,341],"inflammasome":[56,88,101,110,169,206,273,283,299,317],"is":[57,200],"activated":[58],"diverse":[61],"array":[62],"stimuli,":[64],"including":[65],"crystals,":[66],"ATP,":[67,233,253],"pore-forming":[68],"toxins,":[69],"tetanolysin":[72],"O":[73],"(TLO),":[74],"potassium":[77],"ionophore":[78],"nigericin.":[79],"Here,":[80],"we":[81,149,214,305],"have":[82,215],"explored":[83],"mechanism":[85,199],"using":[90,176],"nigericin,":[91,236,250],"TLO":[92,220],"ATP.":[94],"We":[95,166],"found":[96,216],"that":[97,197,217],"nigericin":[98,160],"induced":[99],"in":[103,109,171,225,258,271,293,336],"bone-marrow":[104],"derived":[105],"(BMDM)":[107],"results":[108,224],"dependent":[111,231,342],"lysosomal":[112],"membrane":[113,117],"permeabilization":[114,118],"(LMP),":[115],"mitochondrial":[116,132,269,307,324],"(MMP),":[119],"processing":[122],"IL-1\u03b2.":[126],"All":[127],"these":[129,303,337],"events":[130,157,193],"required":[131],"reactive":[133],"oxygen":[134],"species":[135],"(ROS).":[136],"Through":[137],"combining":[138],"bulk":[139],"biochemical":[140],"assays":[141],"with":[142,183],"live":[143],"cell":[144,174,211,348],"analysis":[145,153],"individual":[147],"cells,":[148],"provide":[150],"kinetic":[152],"sequence":[155,191,228],"resulting":[158,347],"from":[159],"stimulation":[161,221],"LPS":[163],"primed":[164],"BMDM.":[165,259],"recapitulated":[167],"dendritic":[173],"line,":[175],"novel":[178],"system":[179],"D2SC-1":[181],"transduced":[182,186],"ASC.":[184],"These":[185,285],"cells":[187],"undergo":[188],"similar":[190,227],"macrophages,":[195],"confirming":[196],"this":[198],"general":[202],"result":[203],"applicable":[208],"to":[209],"multiple":[210],"types.":[212],"Furthermore,":[213],"ATP":[218],"BMDM":[223],"events.":[232],"but":[234,251,339],"not":[235,252,327],"requires":[237],"P2X7":[238],"for":[239,268,314],"Surprisingly,":[245],"inhibition":[246],"P2X4":[248],"blocked":[249],"induced,":[254],"NLRP3-dependent":[255],"IL-1\u03b2":[256,334],"Our":[260],"work":[261],"has":[262],"demonstrated":[263],"central,":[265],"common":[266],"role":[267],"ROS":[270,308,325],"determined":[276],"kinetics":[278],"organelle":[280,343],"crosstalk":[281],"during":[282],"activation.":[284,300],"data":[286],"place":[287],"both":[288],"mitochondria":[290],"lysosomes":[292],"critical":[295],"position":[296],"controlling":[297],"Based":[301],"on":[302],"results,":[304],"suggest":[306],"potential":[311],"therapeutic":[312],"target":[313],"treating":[315],"related":[318],"Inhibition":[320],"or":[321],"scavenging":[322],"would":[326],"only":[328],"prevent":[329],"effects":[332],"patients,":[338],"also":[340],"damage":[344],"death.":[349]},"counts_by_year":[],"updated_date":"2026-10-27T15:51:10.671819","created_date":"2016-06-24T00:00:00"}
