{"id":"https://openalex.org/W77817783","doi":"https://doi.org/10.1096/fasebj.22.1_supplement.759.14","title":"Use of a Planar Patch Electrode to Measure NBCe1\u2010A Function in Mammalian Cells","display_name":"Use of a Planar Patch Electrode to Measure NBCe1\u2010A Function in Mammalian Cells","publication_year":2008,"publication_date":"2008-03-01","ids":{"openalex":"https://openalex.org/W77817783","doi":"https://doi.org/10.1096/fasebj.22.1_supplement.759.14","mag":"77817783"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.22.1_supplement.759.14","doi":"https://doi.org/10.1096/fasebj.22.1_supplement.759.14","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.22.1_supplement.759.14","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032556810","display_name":"Liyo Kao","orcid":"https://orcid.org/0009-0001-9045-2320","observed_orcids":["https://orcid.org/0009-0001-9045-2320"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Liyo Kao","raw_affiliation_strings":["Department of Medicine UCLA Los Angeles CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine UCLA Los Angeles CA","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024730010","display_name":"Natalia K. Abuladze","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Natalia Abuladze","raw_affiliation_strings":["Department of Medicine UCLA Los Angeles CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine UCLA Los Angeles CA","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Debra Newman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Debra Newman","raw_affiliation_strings":["Department of Medicine UCLA Los Angeles CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine UCLA Los Angeles CA","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100683895","display_name":"Weixin Liu","orcid":"https://orcid.org/0000-0002-1650-0371","observed_orcids":["https://orcid.org/0000-0002-1650-0371"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Weixin Liu","raw_affiliation_strings":["Department of Medicine UCLA Los Angeles CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine UCLA Los Angeles CA","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108486680","display_name":"Alexander Pushkin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alexander Pushkin","raw_affiliation_strings":["Department of Medicine UCLA Los Angeles CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine UCLA Los Angeles CA","institution_ids":["https://openalex.org/I161318765"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5048085772","display_name":"Ira Kurtz","orcid":"https://orcid.org/0000-0003-2834-510X","observed_orcids":["https://orcid.org/0000-0003-2834-510X"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ira Kurtz","raw_affiliation_strings":["Department of Medicine UCLA Los Angeles CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine UCLA Los Angeles CA","institution_ids":["https://openalex.org/I161318765"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I161318765"],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00675055,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"22","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.6230000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.6230000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":0.11400000005960464,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11434","display_name":"Electrochemical Analysis and Applications","score":0.07079999893903732,"subfield":{"id":"https://openalex.org/subfields/1603","display_name":"Electrochemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/planar-patch-clamp","display_name":"planar patch clamp","score":0.8130000233650208},{"id":"https://openalex.org/keywords/sodium-bicarbonate-cotransporter","display_name":"sodium bicarbonate cotransporter","score":0.9120000004768372},{"id":"https://openalex.org/keywords/hek293t-cells","display_name":"HEK293T cells","score":0.8859999775886536},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.824999988079071},{"id":"https://openalex.org/keywords/dids-inhibition","display_name":"DIDS inhibition","score":0.6190000176429749},{"id":"https://openalex.org/keywords/automated-patch-clamp","display_name":"automated patch clamp","score":0.3050000071525574},{"id":"https://openalex.org/keywords/single-unit-recording","display_name":"single-unit recording","score":0.4300000071525574}],"concepts":[{"id":"https://openalex.org/C147944092","wikidata":"https://www.wikidata.org/wiki/Q176366","display_name":"Patch clamp","level":3,"score":0.6529330611228943},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.6166960000991821},{"id":"https://openalex.org/C17525397","wikidata":"https://www.wikidata.org/wiki/Q176140","display_name":"Electrode","level":2,"score":0.5522114634513855},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4325036406517029},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.41986584663391113},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.38680392503738403},{"id":"https://openalex.org/C185263204","wikidata":"https://www.wikidata.org/wiki/Q1154774","display_name":"Electrophysiology","level":2,"score":0.37818992137908936},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.12710034847259521},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1257360279560089},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1174960732460022},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.07659456133842468},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.22.1_supplement.759.14","doi":"https://doi.org/10.1096/fasebj.22.1_supplement.759.14","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.22.1_supplement.759.14","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.4000000059604645,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1509761531","https://openalex.org/W2280608917","https://openalex.org/W2058067222","https://openalex.org/W4401359493","https://openalex.org/W2050700756","https://openalex.org/W2484388","https://openalex.org/W1572665709","https://openalex.org/W28224184","https://openalex.org/W16221460","https://openalex.org/W2042531992"],"abstract_inverted_index":{"Standard":[0],"whole":[1,236],"cell\u2010patch":[2],"clamping":[3],"is":[4],"labor\u2010intensive":[5],"whereas":[6],"automated":[7],"planar":[8,142,258],"patch":[9,19],"clamp":[10,20],"devices":[11],"offer":[12],"the":[13,39,58,84,88,204,252,277],"possibility":[14],"of":[15,60,87,118,166,197,256],"higher":[16],"throughput.":[17],"Planar":[18,153],"electrodes":[21],"are":[22,52],"also":[23],"attractive":[24],"because":[25],"their":[26],"geometry":[27],"facilitates":[28],"obtaining":[29],"electrical":[30],"and":[31,112,215],"optical":[32],"recordings":[33],"simultaneously.":[34],"Previous":[35],"studies":[36,54],"have":[37,56,69],"utilized":[38,77],"Port\u2010a\u2010Patch":[40,80],"system":[41,81],"(Nanion":[42],"Technologies":[43],"GmbH),":[44],"to":[45,82,190,261],"study":[46,262],"ion":[47],"channel":[48],"activity":[49,86],"however":[50],"there":[51],"no":[53],"which":[55,66,146],"demonstrated":[57],"utility":[59],"this":[61,74,177],"approach":[62],"with":[63,156,163],"electrogenic":[64,89,270],"cotransporters,":[65],"in":[67,110,121,272],"general":[68],"a":[70,78,116,141,147,164,194,257,267],"lower":[71],"conductance.":[72],"In":[73],"study,":[75],"we":[76,171],"modified":[79],"measure":[83],"functional":[85],"sodium":[90],"bicarbonate":[91],"cotransporter,":[92],"NBCe1\u2010A.":[93],"HEK\u2010293T":[94,205],"cells":[95,125,137,206],"permanently":[96],"expressing":[97],"NBCe1\u2010A":[98],"were":[99,103,113,126,133,138,159,182],"generated.":[100],"Cell":[101,200],"monolayers":[102],"detached":[104],"using":[105,176],"0.025%":[106],"Trypsin/0.22":[107],"mM":[108],"EDTA":[109],"PBS":[111],"re\u2010suspended":[114],"at":[115,128,246],"concentration":[117],"500,000":[119],"cells/ml":[120],"50%":[122],"DMEM/PBS.":[123],"The":[124,225],"kept":[127],"room":[129],"temperature":[130],"until":[131],"measurements":[132],"made.":[134],"Approximately":[135],"2,500":[136],"placed":[139],"on":[140],"electrode":[143,154],"chip":[144],"from":[145,187,193],"single":[148],"cell":[149,180,237],"was":[150,207,218,239],"automatically":[151],"selected.":[152],"chips":[155,162],"variable":[157],"resistances":[158],"assessed.":[160],"Using":[161],"resistance":[165,217],"2":[167],"\u2013":[168],"3.5":[169],"MOhm,":[170],"routinely":[172],"obtained":[173],"GOhm":[174],"seals":[175],"approach.":[178],"Whole":[179],"currents":[181,264],"elicited":[183],"by":[184,266,276],"voltage":[185],"ramps":[186],"\u2212100":[188,198],"mV":[189,192],"+30":[191,247],"holding":[195],"potential":[196],"mV.":[199,248],"membrane":[201],"capacitance":[202],"for":[203],"7.14":[208],"\u00b1":[209,220,241],"0.67":[210],"pF":[211],"(n":[212,223,244],"=":[213],"21)":[214],"access":[216],"8.6":[219],"0.5":[221],"MOhm":[222],"=21).":[224],"DIDS\u2010inhibitable":[226],"Na":[227],"+":[228],"\u2010and":[229],"HCO":[230],"3":[231],"\u2212":[232],"\u2010dependent":[233],"NBCe1\u2010A\u2010mediated":[234],"average":[235],"current":[238],"29.3":[240],"3.2":[242],"pA":[243],"=21)":[245],"These":[249],"results":[250],"represent":[251],"first":[253],"reported":[254],"use":[255],"patch\u2010clamp":[259],"device":[260],"ionic":[263],"mediated":[265],"heterologously":[268],"expressed":[269],"cotransporter":[271],"mammalian":[273],"cells.":[274],"Supported":[275],"NIH":[278]},"counts_by_year":[],"updated_date":"2026-10-01T08:54:57.152042","created_date":"2016-06-24T00:00:00"}
