{"id":"https://openalex.org/W774373982","doi":null,"title":"Multivariate statistical analysis (MSA) improves azimuthal alignment along the axis of helical objects and results in higher resolution for reconstruction using a single particle approach","display_name":"Multivariate statistical analysis (MSA) improves azimuthal alignment along the axis of helical objects and results in higher resolution for reconstruction using a single particle approach","publication_year":2002,"publication_date":"2002-01-01","ids":{"openalex":"https://openalex.org/W774373982","mag":"774373982"},"language":"en","primary_location":{"id":"pmh:oai:edoc.mpg.de:23114","doi":null,"is_oa":false,"landing_page_url":"http://edoc.mpg.de/23114","pdf_url":null,"source":{"id":"https://openalex.org/S4406922265","display_name":"Max Planck Institute for Plasma Physics","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biophysical Journal (Annual Meeting Abstracts), v.82, 491-491 (2002)","raw_type":"Article"},"volume":null,"type":"conference-abstract","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5110116618","display_name":"Rasmus R. Schr\u00f6der","orcid":"https://orcid.org/0009-0008-6410-7217","observed_orcids":["https://orcid.org/0009-0008-6410-7217"]},"institutions":[{"id":"https://openalex.org/I149899117","display_name":"Max Planck Society","ror":"https://ror.org/01hhn8329","country_code":"DE","type":"nonprofit","lineage":["https://openalex.org/I149899117"]},{"id":"https://openalex.org/I4210087042","display_name":"Max Planck Institute for Medical Research","ror":"https://ror.org/000bxzc63","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210087042"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Rasmus R. Schr\u00f6der","raw_affiliation_strings":["Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society","institution_ids":["https://openalex.org/I149899117","https://openalex.org/I4210087042"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032575518","display_name":"Isabel Angert","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Isabel Angert","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042892654","display_name":"Joachim Frank","orcid":"https://orcid.org/0000-0001-5449-6943","observed_orcids":["https://orcid.org/0000-0001-5449-6943","https://orcid.org/0000-0002-2442-1112"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joachim Frank","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-5449-6943","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5052279487","display_name":"Kenneth Charles Holmes","orcid":"https://orcid.org/0000-0001-8894-9453","observed_orcids":["https://orcid.org/0000-0001-8894-9453"]},"institutions":[{"id":"https://openalex.org/I149899117","display_name":"Max Planck Society","ror":"https://ror.org/01hhn8329","country_code":"DE","type":"nonprofit","lineage":["https://openalex.org/I149899117"]},{"id":"https://openalex.org/I4210087042","display_name":"Max Planck Institute for Medical Research","ror":"https://ror.org/000bxzc63","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210087042"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Kenneth C. Holmes","raw_affiliation_strings":["Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society"],"raw_orcid":"https://orcid.org/0000-0001-8894-9453","affiliations":[{"raw_affiliation_string":"Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society","institution_ids":["https://openalex.org/I149899117","https://openalex.org/I4210087042"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"82","issue":"1","first_page":"443","last_page":"444"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0027000000700354576,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/multivariate-analysis","display_name":"multivariate analysis","score":0.8939999938011169},{"id":"https://openalex.org/keywords/azimuthal-alignment","display_name":"azimuthal alignment","score":0.9570000171661377},{"id":"https://openalex.org/keywords/helical-symmetry","display_name":"helical symmetry","score":0.8169999718666077},{"id":"https://openalex.org/keywords/single-particle-reconstruction","display_name":"single-particle reconstruction","score":0.7509999871253967},{"id":"https://openalex.org/keywords/actin-myosin-complex","display_name":"actin-myosin complex","score":0.9490000009536743},{"id":"https://openalex.org/keywords/resolution-enhancement","display_name":"resolution enhancement","score":0.4569999873638153},{"id":"https://openalex.org/keywords/cryo-electron-tomography","display_name":"cryo-electron tomography","score":0.7900000214576721}],"concepts":[{"id":"https://openalex.org/C161584116","wikidata":"https://www.wikidata.org/wiki/Q1952580","display_name":"Multivariate statistics","level":2,"score":0.67842036485672},{"id":"https://openalex.org/C159737794","wikidata":"https://www.wikidata.org/wiki/Q124274","display_name":"Azimuth","level":2,"score":0.6778495907783508},{"id":"https://openalex.org/C138268822","wikidata":"https://www.wikidata.org/wiki/Q1051925","display_name":"Resolution (logic)","level":2,"score":0.5177806615829468},{"id":"https://openalex.org/C38180746","wikidata":"https://www.wikidata.org/wiki/Q1952580","display_name":"Multivariate analysis","level":2,"score":0.4921061396598816},{"id":"https://openalex.org/C2778517922","wikidata":"https://www.wikidata.org/wiki/Q7140482","display_name":"Particle (ecology)","level":2,"score":0.4729456901550293},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.3831257224082947},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.3394330143928528},{"id":"https://openalex.org/C13280743","wikidata":"https://www.wikidata.org/wiki/Q131089","display_name":"Geodesy","level":1,"score":0.3278370499610901},{"id":"https://openalex.org/C127313418","wikidata":"https://www.wikidata.org/wiki/Q1069","display_name":"Geology","level":0,"score":0.3277853727340698},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3223543167114258},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.3172172009944916},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.31243619322776794},{"id":"https://openalex.org/C105795698","wikidata":"https://www.wikidata.org/wiki/Q12483","display_name":"Statistics","level":1,"score":0.19865331053733826},{"id":"https://openalex.org/C111368507","wikidata":"https://www.wikidata.org/wiki/Q43518","display_name":"Oceanography","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:edoc.mpg.de:23114","doi":null,"is_oa":false,"landing_page_url":"http://edoc.mpg.de/23114","pdf_url":null,"source":{"id":"https://openalex.org/S4406922265","display_name":"Max Planck Institute for Plasma Physics","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biophysical Journal (Annual Meeting Abstracts), v.82, 491-491 (2002)","raw_type":"Article"},{"id":"mag:774373982","doi":null,"is_oa":false,"landing_page_url":"http://pubman.mpdl.mpg.de/pubman/item/escidoc:2247450","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7140241184","https://openalex.org/W2174318090","https://openalex.org/W1979362666","https://openalex.org/W2133991103","https://openalex.org/W2033900859","https://openalex.org/W2003157164","https://openalex.org/W3040278310","https://openalex.org/W2131417227","https://openalex.org/W2951384153","https://openalex.org/W2061679830"],"abstract_inverted_index":{"To":[0],"obtain":[1],"high":[2],"structural":[3],"resolution":[4,41,61],"for":[5,91,101],"reconstruction":[6,171],"of":[7,26,35,67],"helical":[8,20,37],"objects":[9],"by":[10,136],"single":[11],"particle":[12],"methods":[13],"a":[14,29,59,79,86,124,145],"highly":[15],"accurate":[16],"alignment":[17,161],"along":[18,70],"the":[19,27,33,36,92,116,128,138,142,149,157,169],"axis":[21],"is":[22,64,153],"necessary.":[23],"In":[24,144],"case":[25],"actin-myosin-complex":[28],"typical":[30],"misalignment":[31],"on":[32,45,168],"order":[34],"symmetry":[38],"(\u00b112.7\u00b0)":[39],"limits":[40],"to":[42,49,77,98,141,155,162],"1-2.5nm":[43],"depending":[44],"radius.":[46],"Applying":[47],"MSA":[48,83],"conventionally":[50],"aligned":[51],"2D-projections":[52,66],"different":[53,71],"projection":[54,93],"angles":[55,73],"are":[56,176],"distinguished.":[57],"If":[58],"low":[60],"reconstructed":[62],"density":[63,69],"available":[65],"that":[68],"azimuthal":[72,131,160],"can":[74,110,133],"be":[75,113,134],"used":[76],"define":[78],"multi-dimensional":[80],"factor":[81,118],"space.":[82,119],"then":[84,111],"defines":[85],"1D-sub-space":[87],"including":[88],"its":[89],"scale":[90],"angle.":[94],"Matching":[95],"model":[96],"data":[97,100,109,122],"experimental":[99,108,121],"signal":[102],"strength,":[103],"resolution,":[104],"and":[105,172],"CTF,":[106],"these":[107],"directly":[112],"mapped":[114],"into":[115],"given":[117],"The":[120,166],"shows":[123],"close":[125],"distribution":[126],"around":[127],"sub-space.":[129,143],"An":[130],"angle":[132],"assigned":[135],"determining":[137],"smallest":[139],"distance":[140],"first":[146],"application":[147],"using":[148],"actin-myosin-":[150],"complex":[151],"it":[152],"possible":[154],"reduce":[156],"error":[158],"in":[159],"less":[163],"than":[164],"\u00b13\u00b0.":[165],"effect":[167],"final":[170],"further":[173],"refinement":[174],"strategies":[175],"discussed.":[177]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2016-06-24T00:00:00"}