{"id":"https://openalex.org/W77381044","doi":null,"title":"Engineering and characterisation of novel protein covalent linkages in horseradish peroxidase (HRP) : effect on structure and function","display_name":"Engineering and characterisation of novel protein covalent linkages in horseradish peroxidase (HRP) : effect on structure and function","publication_year":2011,"publication_date":"2011-10-19","ids":{"openalex":"https://openalex.org/W77381044","mag":"77381044"},"language":"en","primary_location":{"id":"pmh:oai:sro.sussex.ac.uk:7422","doi":null,"is_oa":true,"landing_page_url":"http://sro.sussex.ac.uk/7422/1/Al-Fartusie%2C_Falah.pdf","pdf_url":"http://sro.sussex.ac.uk/7422/1/Al-Fartusie%2C_Falah.pdf","source":{"id":"https://openalex.org/S4306400129","display_name":"Sussex Research Online (University of Sussex)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I162608824","host_organization_name":"University of Sussex","host_organization_lineage":["https://openalex.org/I162608824"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://sro.sussex.ac.uk/7422/1/Al-Fartusie%2C_Falah.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5053876431","display_name":"Falah Sumoon Daghal Al-Fartusie","orcid":"https://orcid.org/0000-0003-2966-1235","observed_orcids":["https://orcid.org/0000-0003-2966-1235","https://orcid.org/0000-0001-5532-4588"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Falah Sumoon Daghal Al-Fartusie","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5053876431"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.6137999892234802,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.6137999892234802,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.09920000284910202,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.07039999961853027,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/horseradish-peroxidase","display_name":"horseradish peroxidase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/hydrogen-peroxide","display_name":"hydrogen peroxide","score":0.45500001311302185}],"concepts":[{"id":"https://openalex.org/C2777676563","wikidata":"https://www.wikidata.org/wiki/Q413665","display_name":"Horseradish peroxidase","level":3,"score":0.8391361236572266},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7721751928329468},{"id":"https://openalex.org/C162008176","wikidata":"https://www.wikidata.org/wiki/Q419305","display_name":"Peroxidase","level":3,"score":0.6988035440444946},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.6920398473739624},{"id":"https://openalex.org/C533411734","wikidata":"https://www.wikidata.org/wiki/Q171877","display_name":"Hydrogen peroxide","level":2,"score":0.5081021785736084},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.47495245933532715},{"id":"https://openalex.org/C2776217839","wikidata":"https://www.wikidata.org/wiki/Q189621","display_name":"Heme","level":3,"score":0.4729984402656555},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2812454104423523},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.26559334993362427},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.16711843013763428}],"mesh":[],"locations_count":5,"locations":[{"id":"pmh:oai:sro.sussex.ac.uk:7422","doi":null,"is_oa":true,"landing_page_url":"http://sro.sussex.ac.uk/7422/1/Al-Fartusie%2C_Falah.pdf","pdf_url":"http://sro.sussex.ac.uk/7422/1/Al-Fartusie%2C_Falah.pdf","source":{"id":"https://openalex.org/S4306400129","display_name":"Sussex Research Online (University of Sussex)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I162608824","host_organization_name":"University of Sussex","host_organization_lineage":["https://openalex.org/I162608824"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"pmh:hdl:10068/993785","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10068/993785","pdf_url":null,"source":{"id":"https://openalex.org/S4377196900","display_name":"OpenGrey (Institut de l'Information Scientifique et Technique)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1295345811","host_organization_name":"Institut de l'Information Scientifique et Technique","host_organization_lineage":["https://openalex.org/I1295345811"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"U - Thesis"},{"id":"pmh:oai:figshare.com:article/23316752","doi":null,"is_oa":false,"landing_page_url":"https://figshare.com/articles/thesis/Engineering_and_characterisation_of_novel_protein_covalent_linkages_in_horseradish_peroxidase_HRP_effect_on_structure_and_function/23316752","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"pmh:oai:uilis.unsyiah.ac.id:27639","doi":null,"is_oa":false,"landing_page_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/27639","pdf_url":null,"source":{"id":"https://openalex.org/S4377196378","display_name":"UPT. Syiah Kuala University Library (Syiah Kuala University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I187494767","host_organization_name":"Universitas Syiah Kuala","host_organization_lineage":["https://openalex.org/I187494767"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:77381044","doi":null,"is_oa":false,"landing_page_url":"https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.554548","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:sro.sussex.ac.uk:7422","doi":null,"is_oa":true,"landing_page_url":"http://sro.sussex.ac.uk/7422/1/Al-Fartusie%2C_Falah.pdf","pdf_url":"http://sro.sussex.ac.uk/7422/1/Al-Fartusie%2C_Falah.pdf","source":{"id":"https://openalex.org/S4306400129","display_name":"Sussex Research Online (University of Sussex)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I162608824","host_organization_name":"University of Sussex","host_organization_lineage":["https://openalex.org/I162608824"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W77381044.pdf","grobid_xml":"https://content.openalex.org/works/W77381044.grobid-xml"},"referenced_works_count":155,"referenced_works":["https://openalex.org/W49021375","https://openalex.org/W168914787","https://openalex.org/W275444623","https://openalex.org/W641211485","https://openalex.org/W1480600051","https://openalex.org/W1480679012","https://openalex.org/W1491063910","https://openalex.org/W1499745754","https://openalex.org/W1506275008","https://openalex.org/W1508672640","https://openalex.org/W1517408457","https://openalex.org/W1565691495","https://openalex.org/W1567781128","https://openalex.org/W1571455001","https://openalex.org/W1575611248","https://openalex.org/W1576919957","https://openalex.org/W1577138064","https://openalex.org/W1578742674","https://openalex.org/W1585799942","https://openalex.org/W1590638211","https://openalex.org/W1591129997","https://openalex.org/W1595176569","https://openalex.org/W1600112653","https://openalex.org/W1606032544","https://openalex.org/W1623206089","https://openalex.org/W1656759821","https://openalex.org/W1743384604","https://openalex.org/W1757759831","https://openalex.org/W1768117386","https://openalex.org/W1803620352","https://openalex.org/W1849424407","https://openalex.org/W1896483236","https://openalex.org/W1964088665","https://openalex.org/W1964736111","https://openalex.org/W1965493010","https://openalex.org/W1966123947","https://openalex.org/W1968034982","https://openalex.org/W1970125935","https://openalex.org/W1970701255","https://openalex.org/W1972023813","https://openalex.org/W1972824411","https://openalex.org/W1974606151","https://openalex.org/W1974830441","https://openalex.org/W1974959933","https://openalex.org/W1975664813","https://openalex.org/W1976887007","https://openalex.org/W1977460844","https://openalex.org/W1977928693","https://openalex.org/W1979135619","https://openalex.org/W1980712779","https://openalex.org/W1982823065","https://openalex.org/W1983203735","https://openalex.org/W1983572298","https://openalex.org/W1985261459","https://openalex.org/W1987954806","https://openalex.org/W1988719811","https://openalex.org/W1991694882","https://openalex.org/W1991774630","https://openalex.org/W1993257224","https://openalex.org/W1993708276","https://openalex.org/W1994487420","https://openalex.org/W1994667824","https://openalex.org/W1995056740","https://openalex.org/W1996236557","https://openalex.org/W1998282086","https://openalex.org/W1998662190","https://openalex.org/W1998955152","https://openalex.org/W2001591583","https://openalex.org/W2001836344","https://openalex.org/W2005777794","https://openalex.org/W2006289163","https://openalex.org/W2006762876","https://openalex.org/W2006822851","https://openalex.org/W2010094123","https://openalex.org/W2011857157","https://openalex.org/W2012025379","https://openalex.org/W2013226936","https://openalex.org/W2015719071","https://openalex.org/W2016573311","https://openalex.org/W2016830482","https://openalex.org/W2017661443","https://openalex.org/W2018984214","https://openalex.org/W2021001458","https://openalex.org/W2021171040","https://openalex.org/W2023042894","https://openalex.org/W2024117678","https://openalex.org/W2024150007","https://openalex.org/W2028788449","https://openalex.org/W2030920634","https://openalex.org/W2031639594","https://openalex.org/W2033931593","https://openalex.org/W2037131929","https://openalex.org/W2037381371","https://openalex.org/W2038500080","https://openalex.org/W2039042657","https://openalex.org/W2039562073","https://openalex.org/W2042958485","https://openalex.org/W2044705084","https://openalex.org/W2046269757","https://openalex.org/W2049509634","https://openalex.org/W2058954965","https://openalex.org/W2065075153","https://openalex.org/W2065606205","https://openalex.org/W2066345974","https://openalex.org/W2068058822","https://openalex.org/W2070548987","https://openalex.org/W2070856616","https://openalex.org/W2072228771","https://openalex.org/W2072724034","https://openalex.org/W2073089909","https://openalex.org/W2073292063","https://openalex.org/W2074294504","https://openalex.org/W2074919091","https://openalex.org/W2075580314","https://openalex.org/W2075844773","https://openalex.org/W2076594642","https://openalex.org/W2079390178","https://openalex.org/W2080940092","https://openalex.org/W2081613503","https://openalex.org/W2081984559","https://openalex.org/W2082043922","https://openalex.org/W2085266874","https://openalex.org/W2085501958","https://openalex.org/W2086073464","https://openalex.org/W2089186108","https://openalex.org/W2089271899","https://openalex.org/W2091607308","https://openalex.org/W2091937188","https://openalex.org/W2095040101","https://openalex.org/W2103947850","https://openalex.org/W2109173122","https://openalex.org/W2113977229","https://openalex.org/W2121332490","https://openalex.org/W2124124969","https://openalex.org/W2128692282","https://openalex.org/W2128854105","https://openalex.org/W2133110429","https://openalex.org/W2140199154","https://openalex.org/W2142122443","https://openalex.org/W2143685603","https://openalex.org/W2157017796","https://openalex.org/W2160748613","https://openalex.org/W2161273461","https://openalex.org/W2162739847","https://openalex.org/W2166827931","https://openalex.org/W2179092050","https://openalex.org/W2236143335","https://openalex.org/W2252783833","https://openalex.org/W2311358531","https://openalex.org/W2370430095","https://openalex.org/W2490704555","https://openalex.org/W2503184638","https://openalex.org/W2798703673","https://openalex.org/W2950253574","https://openalex.org/W3141008327"],"related_works":["https://openalex.org/W7052995292","https://openalex.org/W63847393","https://openalex.org/W1980727378","https://openalex.org/W1979135619","https://openalex.org/W2065606205","https://openalex.org/W1991774630","https://openalex.org/W2037381371","https://openalex.org/W2030899562","https://openalex.org/W2531287947","https://openalex.org/W2008704566"],"abstract_inverted_index":{"Both":[0],"mammalian":[1],"and":[2,67,71,112,116,149,219,280,290,299,329,539,545],"bacterial":[3],"peroxidases":[4],"contain":[5],"novel":[6,119],"covalent":[7,76,422],"linkages.":[8,77],"In":[9,104,178],"the":[10,24,28,31,37,69,81,127,161,180,185,190,200,207,214,222,231,235,248,254,261,271,275,304,314,319,322,343,346,354,358,374,388,414,433,455,461,472,484,494,499,503,515,540,564],"former":[11],"a":[12,16,117,131,166,210,251,267,337,402,420],"sulfonium":[13,359],"linkage":[14,121,277,360,556],"to":[15,22,65,91,156,206,247,318,351,369,466,470,522,535,563],"critical":[17],"Met":[18,92],"residue":[19,404],"is":[20,408],"thought":[21],"modify":[23],"normal":[25],"planarity":[26],"of":[27,39,74,89,126,184,199,213,234,253,270,306,313,345,353,390,413,432,471,483,493,502,517,519,530],"haem":[29,232,255,298,315,415],"affecting":[30],"functional":[32],"properties.":[33],"Following":[34],"on":[35,99],"from":[36,260],"work":[38,79],"Metcalfe":[40],"et":[41],"al.,":[42],"2004":[43],"which":[44],"showed":[45,333],"that":[46,87,198,266,334,352,401,437],"such":[47],"links":[48],"could":[49,238,256,379],"be":[50,240,257,381],"engineered":[51,75,565],"in":[52,80,93,96,175,245,303,321,342,387,427,454,514],"ascorbate":[53],"peroxidase,":[54],"horseradish":[55],"peroxidase":[56,393],"(HRP)":[57],"has":[58,435,542],"been":[59,543],"used":[60],"as":[61,324,395,508],"convenient":[62],"model":[63],"system":[64],"try":[66],"understand":[68],"structural":[70,120],"electronic":[72],"effects":[73],"Previous":[78],"group":[82,233],"(Cali,":[83],"2008)":[84],"had":[85,273],"shown":[86,436],"mutation":[88],"Ser167":[90],"HRP-C*":[94],"resulted":[95],"autocatalytic":[97],"cross-linking":[98],"incubation":[100],"with":[101,165,364,411,506],"hydrogen":[102,225,365],"peroxide.":[103],"this":[105,371,571],"thesis,":[106],"two":[107],"additional":[108,559],"HRP":[109],"variants,":[110],"S167Y":[111,128,148,201,236,263,348,377,456,486,531],"S167W":[113,186],"were":[114,141,284],"studied":[115],"new":[118],"discovered.":[122],"The":[123,137,297,327,480,526,550],"UV/Vis":[124],"spectrum":[125,332],"variant":[129,187,202,237,457,487,532],"suggested":[130],"more":[132,167],"6-coordinate":[133],"high":[134,170],"spin":[135,171],"character.":[136],"molar":[138],"extinction":[139],"coefficients":[140],"markedly":[142],"increased,":[143],"180":[144],"mM-1":[145,151,158],"cm-1":[146,152,159],"for":[147,153,160,189,498],"135":[150],"S167W,":[154],"compared":[155,350,469],"100":[157],"WT":[162,446,473],"enzyme,":[163,474],"consistent":[164],"6":[168],"coordinate":[169],"character":[172],"normally":[173,443],"seen":[174,426],"lignin":[176],"peroxidase.":[177],"contrast,":[179],"dissociation":[181],"constant":[182],"(Kd)":[183],"mutant":[188],"aromatic":[191,215],"donor":[192,216],"BHA":[193],"was":[194,316,336,367,385,440,451,477,488,533],"hardly":[195],"affected,":[196],"whilst":[197],"increased":[203],"two-fold":[204],"relative":[205],"WT,":[208],"implying":[209,265],"significant":[211],"perturbation":[212],"binding":[217],"site":[218],"/":[220,292],"or":[221,459],"associated":[223],"haem-linked":[224],"bonding":[226],"network.":[227],"After":[228],"peroxide":[229,307,366],"treatment":[230,363],"not":[239,424],"extracted":[241,258],"into":[242],"acid":[243],"butanone":[244],"contrast":[246],"WT.":[249],"Only":[250],"proportion":[252],"even":[259],"untreated":[262,323],"variant,":[264],"substantial":[268],"fraction":[269],"protein":[272,300,320,378],"formed":[274,441],"haem-protein":[276],"during":[278,286],"folding":[279],"purification.":[281],"These":[282],"results":[283],"confirmed":[285],"reverse":[287],"phase":[288],"HPLC":[289],"MALDI-TOF":[291,328],"ESI":[293,330,567],"mass":[294,331,344,568],"spectroscopy":[295],"measurements.":[296],"completely":[301],"co-eluted":[302],"case":[305],"treated":[308],"S167Y,":[309],"while":[310],"only":[311,380],"~50%":[312],"linked":[317,347],"isolated":[325,382],"enzyme.":[326,356],"there":[335],"large":[338],"increase":[339,513],"(614":[340],"Da)":[341],"protein,":[349],"unlinked":[355,376],"Unlike":[357],"obtained":[361],"earlier,":[362],"unnecessary":[368],"observe":[370],"increase.":[372],"Interestingly,":[373],"100%":[375],"if":[383],"enzyme":[384],"prepared":[386],"presence":[389],"an":[391,396,512,554,558],"efficient":[392],"substrate":[394,481,510],"antioxidant":[397],"scavenger.":[398],"It":[399],"appears":[400],"Tyr":[403],"at":[405,444],"position":[406],"167":[407],"highly":[409],"reactive":[410],"respect":[412],"vinyl":[416],"side":[417],"chain":[418],"forming":[419],"spontaneous":[421],"link":[423],"otherwise":[425],"nature.":[428],"Pre":[429],"steady-state":[430],"comparison":[431],"intermediates":[434],"Compound":[438,520,523],"I":[439,521],"essentially":[442],"near":[445],"rates,":[447],"however":[448],"its":[449],"stability":[450],"greatly":[452],"affected":[453],"(linked":[458],"unlinked),":[460],"life":[462],"time":[463],"being":[464],"decreased":[465],"~0.04":[467],"s,":[468],"where":[475],"it":[476],"~80":[478],"s.":[479],"preference":[482],"cross-linked":[485],"also":[489],"altered.":[490],"Stopped-flow":[491],"measurements":[492,569],"individual":[495],"rate":[496,516],"constants":[497],"partial":[500],"reactions":[501],"catalytic":[504],"cycle":[505],"luminol":[507],"reducing":[509],"revealed":[511],"reduction":[518],"II":[524],"(k2).":[525],"X-ray":[527],"crystal":[528],"structure":[529,541,552,561],"solved":[534],"1.7":[536],"A\u030a":[537],"resolution":[538],"modelled":[544],"determined":[546],"by":[547],"x-ray":[548,551],"crystallography.":[549],"reveals":[553],"unanticipated":[555],"containing":[557],"ring":[560],"bonded":[562],"Tyr.":[566],"supported":[570],"structure.":[572]},"counts_by_year":[{"year":2024,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}