{"id":"https://openalex.org/W76784570","doi":"https://doi.org/10.1016/s0021-9258(19)45081-3","title":"Purification and Properties of p-Hydroxybenzoate Hydroxylase from Pseudomonas fluorescens","display_name":"Purification and Properties of p-Hydroxybenzoate Hydroxylase from Pseudomonas fluorescens","publication_year":1972,"publication_date":"1972-07-01","ids":{"openalex":"https://openalex.org/W76784570","doi":"https://doi.org/10.1016/s0021-9258(19)45081-3","mag":"76784570","pmid":"https://pubmed.ncbi.nlm.nih.gov/4402514"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(19)45081-3","doi":"https://doi.org/10.1016/s0021-9258(19)45081-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)45081-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(19)45081-3","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060457975","display_name":"Larry G. Howell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Larry G. Howell","raw_affiliation_strings":["From the Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108497790","display_name":"Thomas Spector","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas Spector","raw_affiliation_strings":["From the Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104","institution_ids":["https://openalex.org/I27837315"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5079221179","display_name":"Vincent Massey","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I27837315","display_name":"University of Michigan","ror":"https://ror.org/00jmfr291","country_code":"US","type":"education","lineage":["https://openalex.org/I27837315"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vincent Massey","raw_affiliation_strings":["From the Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104","institution_ids":["https://openalex.org/I27837315"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I27837315"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":10.0647,"has_fulltext":false,"cited_by_count":161,"citation_normalized_percentile":{"value":0.98800091,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"247","issue":"13","first_page":"4340","last_page":"4350"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.23240000009536743,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.23240000009536743,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.219200000166893,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10324","display_name":"Microbial bioremediation and biosurfactants","score":0.149399995803833,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/p-hydroxybenzoate-hydroxylase","display_name":"p-hydroxybenzoate hydroxylase","score":0.996999979019165},{"id":"https://openalex.org/keywords/pseudomonas-fluorescens","display_name":"Pseudomonas fluorescens","score":0.984000027179718},{"id":"https://openalex.org/keywords/protocatechuic-acid","display_name":"protocatechuic acid","score":0.6930000185966492},{"id":"https://openalex.org/keywords/flavin-adenine-dinucleotide","display_name":"flavin adenine dinucleotide","score":0.9589999914169312},{"id":"https://openalex.org/keywords/flavin-reduction","display_name":"flavin reduction","score":0.5479999780654907},{"id":"https://openalex.org/keywords/substrate-binding","display_name":"substrate binding","score":0.746999979019165},{"id":"https://openalex.org/keywords/substrate-inhibition","display_name":"substrate inhibition","score":0.8960000276565552},{"id":"https://openalex.org/keywords/enzyme-activation","display_name":"enzyme activation","score":0.5609999895095825},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.36899998784065247},{"id":"https://openalex.org/keywords/cofactor-free-enzyme","display_name":"cofactor-free enzyme","score":0.5180000066757202},{"id":"https://openalex.org/keywords/ferricyanide-reduction","display_name":"ferricyanide reduction","score":0.699999988079071}],"concepts":[{"id":"https://openalex.org/C2776835041","wikidata":"https://www.wikidata.org/wiki/Q135292","display_name":"Pseudomonas fluorescens","level":3,"score":0.8639203310012817},{"id":"https://openalex.org/C2909186759","wikidata":"https://www.wikidata.org/wiki/Q5955538","display_name":"Hydroxybenzoate","level":2,"score":0.7058709859848022},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5408220291137695},{"id":"https://openalex.org/C2781463711","wikidata":"https://www.wikidata.org/wiki/Q131143","display_name":"Pseudomonas","level":3,"score":0.4711061120033264},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3540486693382263},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.32393649220466614},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2764118015766144},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.11256158351898193},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09288081526756287}],"mesh":[{"descriptor_ui":"D001565","descriptor_name":"Benzoates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003460","descriptor_name":"Crystallization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004355","descriptor_name":"Drug Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004578","descriptor_name":"Electron Spin Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004579","descriptor_name":"Electron Transport","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004586","descriptor_name":"Electrophoresis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004589","descriptor_name":"Electrophoresis, Disc","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005292","descriptor_name":"Ferricyanides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005453","descriptor_name":"Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006899","descriptor_name":"Mixed Function Oxygenases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D006900","descriptor_name":"Hydroxylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008433","descriptor_name":"Mathematics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008956","descriptor_name":"Models, Chemical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009249","descriptor_name":"NADP","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009584","descriptor_name":"Nitrogen","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010100","descriptor_name":"Oxygen","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011549","descriptor_name":"Pseudomonas","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D011551","descriptor_name":"Pseudomonas fluorescens","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013053","descriptor_name":"Spectrophotometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(19)45081-3","doi":"https://doi.org/10.1016/s0021-9258(19)45081-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)45081-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:4402514","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4402514","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(19)45081-3","doi":"https://doi.org/10.1016/s0021-9258(19)45081-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(19)45081-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.6200000047683716,"id":"https://metadata.un.org/sdg/6","display_name":"Clean water and sanitation"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":21,"referenced_works":["https://openalex.org/W26808833","https://openalex.org/W228237819","https://openalex.org/W324317825","https://openalex.org/W1506043065","https://openalex.org/W1544070587","https://openalex.org/W1550154378","https://openalex.org/W1606526641","https://openalex.org/W1750773183","https://openalex.org/W1775749144","https://openalex.org/W1919505456","https://openalex.org/W1978611227","https://openalex.org/W1989279263","https://openalex.org/W1989319480","https://openalex.org/W2006585470","https://openalex.org/W2014667345","https://openalex.org/W2029309737","https://openalex.org/W2030566674","https://openalex.org/W2084114379","https://openalex.org/W2321972685","https://openalex.org/W2329764685","https://openalex.org/W2401927159"],"related_works":["https://openalex.org/W1606526641","https://openalex.org/W205498636","https://openalex.org/W1673729525","https://openalex.org/W1849264732","https://openalex.org/W1534164522","https://openalex.org/W1554198147","https://openalex.org/W2014918380","https://openalex.org/W2312485323","https://openalex.org/W2009688026","https://openalex.org/W2093341604"],"abstract_inverted_index":{"The":[0,36,44,107,133,231,251,265],"inducible":[1],"p-hydroxybenzoate":[2,12,61,136,138,146,254],"hydroxylase":[3,139,255],"of":[4,11,33,49,55,60,92,102,110,122,131,135,145,189,210,216,253,273],"Pseudomonas":[5],"fluorescens":[6],"which":[7,26,165],"catalyzes":[8],"the":[9,31,58,62,85,89,100,103,111,114,120,123,129,168,183,190,200,211,214,246,271,274],"conversion":[10],"to":[13,41,137,167,187],"protocatechuate":[14],"has":[15],"been":[16],"obtained":[17],"in":[18,30,113,128,173,213,235],"crystalline":[19],"form":[20],"as":[21],"an":[22],"electrophoretically":[23],"homogenous":[24],"protein":[25],"is":[27,39,65,117,126,196,258,282],"very":[28],"stable":[29],"absence":[32],"added":[34],"cofactors.":[35],"molecular":[37,204],"weight":[38],"estimated":[40],"be":[42],"65,000.":[43],"enzyme":[45,86,112,184,212],"contains":[46],"1":[47],"mole":[48,54],"flavin":[50,64,125,192,276],"adenine":[51],"dinucleotide":[52],"per":[53],"protein.":[56],"In":[57],"presence":[59,101,130,215],"enzyme-bound":[63,191,275],"rapidly":[66],"reduced":[67,75],"by":[68,72,94,193,203,263,277,285],"TPNH,":[69,194],"but":[70,195],"not":[71,197],"DPNH":[73],"or":[74],"acetyl":[76],"pyridine":[77],"nucleotide.":[78],"Under":[79],"both":[80],"anaerobic":[81,208],"and":[82,119],"aerobic":[83],"conditions":[84],"can":[87],"catalyze":[88],"rapid":[90,97],"reduction":[91,188,209,272],"ferricyanide":[93],"TPNH.":[95],"This":[96],"reaction":[98],"requires":[99],"aromatic":[104],"substrate.":[105],"absorption":[108],"spectrum":[109],"visible":[115],"region":[116],"perturbed":[118],"fluorescence":[121],"enzymebound":[124],"quenched":[127],"p-hydroxybenzoate.":[132,264,286],"binding":[134,142,162],"follows":[140],"1:1":[141,161],"at":[143,260],"concentrations":[144],"less":[147],"than":[148],"3":[149],"x":[151],"10-4":[153],"m":[154],".":[155],"At":[156],"higher":[157],"concentrations,":[158],"deviations":[159],"from":[160],"are":[163,222,233,243],"observed":[164,172],"correspond":[166],"excess":[169],"substrate":[170,179],"inhibition":[171],"steady":[174],"state":[175],"kinetic":[176],"analysis.":[177],"A":[178],"analogue,":[180],"6-hydroxynicotinate,":[181],"activates":[182],"with":[185,245],"respect":[186],"hydroxylated":[198],"during":[199,228],"subsequent":[201],"reoxidation":[202],"oxygen.":[205],"During":[207],"p-hydroxybenzoate,":[217],"two":[218],"transient":[219,247],"spectral":[220,248],"intermediates":[221,225,232],"observable.":[223],"These":[224],"also":[226],"occur":[227],"catalytic":[229],"turnover.":[230],"generated":[234],"sequential":[236],"fashion.":[237],"No":[238],"electron":[239],"paramagnetic":[240],"resonance":[241],"signals":[242],"associated":[244],"intermediates.":[249],"affinity":[252],"for":[256,270],"TPNH":[257,279],"enhanced":[259],"least":[261],"9-fold":[262],"first":[266],"order":[267],"rate":[268],"constant":[269],"bound":[278],"(i.e.":[280],"kred)":[281],"increased":[283],"40,000-fold":[284]},"counts_by_year":[{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
