{"id":"https://openalex.org/W761197398","doi":null,"title":"Express\u00e3o, purifica\u00e7\u00e3o e estudos estruturais da chaperona molecular Hsp65 de Mycobacterium leprae & caracteriza\u00e7\u00e3o da prote\u00edna NUDEL - nuclear distribuition element-like","display_name":"Express\u00e3o, purifica\u00e7\u00e3o e estudos estruturais da chaperona molecular Hsp65 de Mycobacterium leprae & caracteriza\u00e7\u00e3o da prote\u00edna NUDEL - nuclear distribuition element-like","publication_year":2008,"publication_date":"2008-04-18","ids":{"openalex":"https://openalex.org/W761197398","mag":"761197398"},"language":"pt","primary_location":{"id":"pmh:oai:americanae.aecid.es:1741865","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11449/99875","pdf_url":null,"source":{"id":"https://openalex.org/S4306400786","display_name":"Americanae (AECID Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"reponame:Reposit\u00f3rio Institucional da UNESP","raw_type":"info:eu-repo/semantics/masterThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/11449/99875","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032226380","display_name":"Fl\u00e1via Barboza Camargo","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Fl\u00e1via Barboza Camargo","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5032226380"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12107","display_name":"Leprosy Research and Treatment","score":0.008299999870359898,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hsp65","display_name":"hsp65","score":0.9599999785423279},{"id":"https://openalex.org/keywords/mycobacterium-leprae","display_name":"Mycobacterium leprae","score":0.9779999852180481},{"id":"https://openalex.org/keywords/molecular-chaperones","display_name":"molecular chaperones","score":0.5120000243186951},{"id":"https://openalex.org/keywords/heat-shock-proteins","display_name":"heat shock proteins","score":0.8730000257492065},{"id":"https://openalex.org/keywords/protein-expression-and-purification","display_name":"protein expression and purification","score":0.7770000100135803},{"id":"https://openalex.org/keywords/protein-tertiary-structure","display_name":"protein tertiary structure","score":0.6909999847412109},{"id":"https://openalex.org/keywords/nudel","display_name":"Nudel","score":0.531000018119812},{"id":"https://openalex.org/keywords/tuberculosis-antigens","display_name":"tuberculosis antigens","score":0.6729999780654907},{"id":"https://openalex.org/keywords/protein-crystallization","display_name":"protein crystallization","score":0.4390000104904175},{"id":"https://openalex.org/keywords/circular-dichroism","display_name":"circular dichroism","score":0.7300000190734863},{"id":"https://openalex.org/keywords/dynamic-light-scattering","display_name":"dynamic light scattering","score":0.9919999837875366},{"id":"https://openalex.org/keywords/leprosy","display_name":"leprosy","score":0.5080000162124634},{"id":"https://openalex.org/keywords/tuberculosis","display_name":"tuberculosis","score":0.3089999854564667},{"id":"https://openalex.org/keywords/immunogenic-antigens","display_name":"immunogenic antigens","score":0.46299999952316284}],"concepts":[{"id":"https://openalex.org/C2779823659","wikidata":"https://www.wikidata.org/wiki/Q155891","display_name":"Mycobacterium leprae","level":3,"score":0.49752166867256165},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4641721248626709},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4083611071109772},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4055894613265991},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4014224410057068},{"id":"https://openalex.org/C2780695269","wikidata":"https://www.wikidata.org/wiki/Q36956","display_name":"Leprosy","level":2,"score":0.21081721782684326},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.07068407535552979}],"mesh":[],"locations_count":4,"locations":[{"id":"pmh:oai:americanae.aecid.es:1741865","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11449/99875","pdf_url":null,"source":{"id":"https://openalex.org/S4306400786","display_name":"Americanae (AECID Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"reponame:Reposit\u00f3rio Institucional da UNESP","raw_type":"info:eu-repo/semantics/masterThesis"},{"id":"pmh:oai:repositorio.unesp.br:11449/99875","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11449/99875","pdf_url":null,"source":{"id":"https://openalex.org/S4306402641","display_name":"LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Cient\u00edficas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4383465926","host_organization_name":"LA Referencia","host_organization_lineage":["https://openalex.org/I4383465926"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"instacron:UNESP","raw_type":"info:eu-repo/semantics/masterThesis"},{"id":"pmh:oai:acervodigital.unesp.br:11449/99875","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11449/99875","pdf_url":"http://hdl.handle.net/11449/99875","source":{"id":"https://openalex.org/S4306400256","display_name":"Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210115194","host_organization_name":"Funda\u00e7\u00e3o para o Desenvolvimento da UNESP","host_organization_lineage":["https://openalex.org/I4210115194"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"outro"},{"id":"mag:761197398","doi":null,"is_oa":false,"landing_page_url":"http://repositorio.unesp.br/handle/11449/99875","pdf_url":null,"source":{"id":"https://openalex.org/S4210186100","display_name":"Aleph UCLA Undergraduate Research Journal for the Humanities and Social Sciences","issn_l":"2639-6440","issn":["2639-6440","2639-6459"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Aleph UCLA Undergraduate Research Journal for the Humanities and Social Sciences","raw_type":null}],"best_oa_location":{"id":"pmh:oai:americanae.aecid.es:1741865","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11449/99875","pdf_url":null,"source":{"id":"https://openalex.org/S4306400786","display_name":"Americanae (AECID Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"reponame:Reposit\u00f3rio Institucional da UNESP","raw_type":"info:eu-repo/semantics/masterThesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W761197398.pdf","grobid_xml":"https://content.openalex.org/works/W761197398.grobid-xml"},"referenced_works_count":69,"referenced_works":["https://openalex.org/W1463297302","https://openalex.org/W1490197708","https://openalex.org/W1515599953","https://openalex.org/W1545622372","https://openalex.org/W1592760947","https://openalex.org/W1626833318","https://openalex.org/W1859758480","https://openalex.org/W1896987527","https://openalex.org/W1963671313","https://openalex.org/W1964127380","https://openalex.org/W1974101511","https://openalex.org/W1989026158","https://openalex.org/W1989419578","https://openalex.org/W1992502425","https://openalex.org/W1993418823","https://openalex.org/W1996317885","https://openalex.org/W1999654504","https://openalex.org/W2000029034","https://openalex.org/W2001736616","https://openalex.org/W2005796285","https://openalex.org/W2012648981","https://openalex.org/W2020237866","https://openalex.org/W2020622526","https://openalex.org/W2032455585","https://openalex.org/W2032866243","https://openalex.org/W2036538226","https://openalex.org/W2036623899","https://openalex.org/W2037128898","https://openalex.org/W2037835429","https://openalex.org/W2042325100","https://openalex.org/W2046882304","https://openalex.org/W2050430115","https://openalex.org/W2053233981","https://openalex.org/W2055043387","https://openalex.org/W2057033926","https://openalex.org/W2057646888","https://openalex.org/W2066713988","https://openalex.org/W2067459929","https://openalex.org/W2067890396","https://openalex.org/W2070803308","https://openalex.org/W2071090720","https://openalex.org/W2075183688","https://openalex.org/W2082301907","https://openalex.org/W2087636085","https://openalex.org/W2088937623","https://openalex.org/W2093363441","https://openalex.org/W2094576781","https://openalex.org/W2097088842","https://openalex.org/W2100480405","https://openalex.org/W2100837269","https://openalex.org/W2105051672","https://openalex.org/W2105851524","https://openalex.org/W2112007464","https://openalex.org/W2116805879","https://openalex.org/W2119999468","https://openalex.org/W2120302751","https://openalex.org/W2123439296","https://openalex.org/W2124773215","https://openalex.org/W2127551731","https://openalex.org/W2130923453","https://openalex.org/W2132417094","https://openalex.org/W2135772494","https://openalex.org/W2138222426","https://openalex.org/W2144034070","https://openalex.org/W2144874509","https://openalex.org/W2158023121","https://openalex.org/W2171686853","https://openalex.org/W2186839060","https://openalex.org/W2396632380"],"related_works":["https://openalex.org/W2360770980","https://openalex.org/W2001752841","https://openalex.org/W7060821698","https://openalex.org/W2388393872","https://openalex.org/W7099567690","https://openalex.org/W2130923453","https://openalex.org/W2356248859","https://openalex.org/W2942868128","https://openalex.org/W4390517649","https://openalex.org/W2907325475"],"abstract_inverted_index":{"A":[0,66,90,222],"tuberculose":[1,26],"\u00e9":[2,35,71,95],"uma":[3,121],"doen\u00e7a":[4],"infecciosa":[5],"que":[6,114,205],"representa":[7],"um":[8,211,219],"dos":[9],"principais":[10],"problemas":[11],"sociais,":[12],"econ\u00f4micos":[13],"e":[14,30,53,75,104,136,147,231],"de":[15,42,50,92,117,124,140,145,157,167,177,183,194,197,215,224,241,245],"sa\u00fade":[16],"p\u00fablica":[17],"do":[18,108,255],"mundo.":[19],"Muitos":[20],"ant\u00edgenos":[21],"potentes":[22],"demonstraram":[23],"prote\u00e7\u00e3o":[24],"contra":[25],"nos":[27],"modelos":[28],"experimentais":[29],"o":[31,165,181],"mais":[32],"recentemente":[33],"estudado":[34],"a":[36,73,115,134,161,175,185,192,206],"heat":[37],"shock":[38],"protein":[39],"Hsp65":[40,91,116,139,189],"kDa":[41],"Mycobacterium":[43,93],"leprae.":[44],"Situa\u00e7\u00f5es":[45],"como":[46,87,131],"altas":[47],"temperaturas,":[48],"presen\u00e7a":[49,176],"radicais":[51],"livres":[52],"defici\u00eancias":[54],"nutricionais":[55],"levam":[56],"ao":[57,155],"aumento":[58],"da":[59,138,163,188],"produ\u00e7\u00e3o":[60],"das":[61,69,77],"Heat":[62],"Shock":[63],"Proteins":[64],"(Hsp\u00b4s).":[65],"principal":[67,133],"fun\u00e7\u00e3o":[68,76],"Hsp\u00b4s":[70],"manter":[72],"estrutura":[74,178,186],"prote\u00ednas":[78],"e,":[79],"conseq\u00fcentemente,":[80],"preservar":[81],"as":[82],"fun\u00e7\u00f5es":[83],"celulares":[84,105],"essenciais,":[85],"agindo":[86],"chaperonas":[88],"moleculares.":[89],"leprae":[94,119,142],"altamente":[96],"imunog\u00eanica,":[97],"acarretando":[98],"em":[99,143,229,239],"infec\u00e7\u00f5es":[100],"micobacterianas,":[101],"rea\u00e7\u00f5es":[102],"humorais":[103],"por":[106],"parte":[107],"hospedeiro.":[109],"Al\u00e9m":[110,254],"disso,":[111],"foi":[112,171,200],"evidenciada":[113],"M.":[118,141],"apresenta":[120],"poss\u00edvel":[122],"atividade":[123],"oligopeptidase.":[125],"Dessa":[126],"forma,":[127],"esse":[128],"projeto":[129,256],"teve":[130],"alvo":[132],"express\u00e3o":[135],"purifica\u00e7\u00e3o":[137],"grau":[144],"pureza":[146],"quantidade":[148],"adequada":[149],"para":[150,160,173,250],"realizar":[151],"estudos":[152,252],"estruturais.":[153,253],"Devido":[154],"uso":[156],"condi\u00e7\u00f5es":[158,237],"desnaturantes":[159],"solubiliza\u00e7\u00e3o":[162],"prote\u00edna,":[164],"espectro":[166],"Dicro\u00edsmo":[168],"Circular":[169],"(CD)":[170],"utilizado":[172],"analisar":[174],"secund\u00e1ria.":[179],"Com":[180],"objetivo":[182],"investigar":[184],"terci\u00e1ria":[187],"(Cpn":[190],"60-2),":[191],"t\u00e9cnica":[193],"Espalhamento":[195],"Din\u00e2mico":[196],"Luz":[198],"(DLS)":[199],"realizada":[201],"tendo":[202],"sido":[203],"observado":[204],"solu\u00e7\u00e3o":[207],"apresenta-se":[208],"monodispersa":[209],"com":[210],"raio":[212],"hidrodin\u00e2mico":[213],"m\u00e9dio":[214],"10":[216],"nm,":[217],"caracterizando":[218],"estado":[220],"trim\u00e9rico.":[221],"tentativa":[223,244],"cristaliza\u00e7\u00e3o":[225],"dessa":[226],"amostra":[227],"est\u00e1":[228],"andamento":[230],"sob":[232],"intensa":[233],"observa\u00e7\u00e3o.":[234],"Algumas":[235],"outras":[236],"est\u00e3o":[238],"fase":[240],"refinamento":[242],"na":[243],"se":[246],"conseguir":[247],"cristais":[248],"difrat\u00e1veis":[249],"os":[251],"principal,...":[257]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}