{"id":"https://openalex.org/W75954296","doi":null,"title":"Herpes simplex virus type 1 transcription is not detectable in quiescent human stromal keratitis by in situ hybridization.","display_name":"Herpes simplex virus type 1 transcription is not detectable in quiescent human stromal keratitis by in situ hybridization.","publication_year":1993,"publication_date":"1993-02-01","ids":{"openalex":"https://openalex.org/W75954296","mag":"75954296","pmid":"https://pubmed.ncbi.nlm.nih.gov/8382667"},"language":"en","primary_location":{"id":"pmid:8382667","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8382667","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085030360","display_name":"Keith A. Laycock","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"K A Laycock","raw_affiliation_strings":["Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, Missouri 63110"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, Missouri 63110","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025199577","display_name":"S F Lee","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S F Lee","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045608707","display_name":"R. Doyle Stulting","orcid":"https://orcid.org/0000-0003-3635-1913","observed_orcids":["https://orcid.org/0000-0003-3635-1913"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R D Stulting","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078707160","display_name":"Kenneth D. Croen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K D Croen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080139851","display_name":"Jeffrey M. Ostrove","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J M Ostrove","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113580060","display_name":"Stephen E. Straus","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S E Straus","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5022918429","display_name":"Jay S. Pepose","orcid":"https://orcid.org/0000-0002-1339-5123","observed_orcids":["https://orcid.org/0000-0002-1339-5123"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J S Pepose","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.9946,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.75186089,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"34","issue":"2","first_page":"285","last_page":"92"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10625","display_name":"Herpesvirus Infections and Treatments","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10625","display_name":"Herpesvirus Infections and Treatments","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11826","display_name":"Ocular Infections and Treatments","score":0.0066999997943639755,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10563","display_name":"Corneal surgery and disorders","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/herpes-simplex-virus-type-1","display_name":"herpes simplex virus type 1","score":0.9940000176429749},{"id":"https://openalex.org/keywords/herpetic-stromal-keratitis","display_name":"herpetic stromal keratitis","score":0.9089999794960022},{"id":"https://openalex.org/keywords/corneal-scarring","display_name":"corneal scarring","score":0.5709999799728394},{"id":"https://openalex.org/keywords/viral-latency","display_name":"viral latency","score":0.7229999899864197},{"id":"https://openalex.org/keywords/latency-associated-transcript","display_name":"latency-associated transcript","score":0.8539999723434448},{"id":"https://openalex.org/keywords/corneal-tissue","display_name":"corneal tissue","score":0.5070000290870667}],"concepts":[{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.8849195837974548},{"id":"https://openalex.org/C2781196997","wikidata":"https://www.wikidata.org/wiki/Q655331","display_name":"Herpes simplex virus","level":3,"score":0.7618961334228516},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6769459247589111},{"id":"https://openalex.org/C182615771","wikidata":"https://www.wikidata.org/wiki/Q1369386","display_name":"Lytic cycle","level":3,"score":0.6509820222854614},{"id":"https://openalex.org/C2778560704","wikidata":"https://www.wikidata.org/wiki/Q757838","display_name":"Keratitis","level":2,"score":0.6342588663101196},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.6135350465774536},{"id":"https://openalex.org/C2776409557","wikidata":"https://www.wikidata.org/wiki/Q149348","display_name":"Herpesviridae","level":4,"score":0.5766420960426331},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5296167135238647},{"id":"https://openalex.org/C16930146","wikidata":"https://www.wikidata.org/wiki/Q4381253","display_name":"Stromal cell","level":2,"score":0.5276262164115906},{"id":"https://openalex.org/C2776882836","wikidata":"https://www.wikidata.org/wiki/Q168291","display_name":"Cornea","level":2,"score":0.4895358979701996},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.44412821531295776},{"id":"https://openalex.org/C2777822432","wikidata":"https://www.wikidata.org/wiki/Q216681","display_name":"In situ","level":2,"score":0.43688151240348816},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3965578079223633},{"id":"https://openalex.org/C2780727368","wikidata":"https://www.wikidata.org/wiki/Q1928978","display_name":"Viral disease","level":3,"score":0.25713446736335754},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2435271441936493},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.17782479524612427},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.15072929859161377},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.12985658645629883},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.10698851943016052},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000956","descriptor_name":"Antigens, Viral","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003315","descriptor_name":"Cornea","qualifier_ui":"Q000294","qualifier_name":"innervation","is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D005814","descriptor_name":"Genes, Viral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009693","descriptor_name":"Nucleic Acid Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012668","descriptor_name":"Trigeminal Ganglion","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014709","descriptor_name":"Vero Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015347","descriptor_name":"RNA Probes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015948","descriptor_name":"Keratoplasty, Penetrating","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016849","descriptor_name":"Keratitis, Herpetic","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D018139","descriptor_name":"Simplexvirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D018139","descriptor_name":"Simplexvirus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false}],"locations_count":3,"locations":[{"id":"pmid:8382667","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8382667","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.319.7781","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.319.7781","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.iovs.org/content/34/2/285.full.pdf","raw_type":"text"},{"id":"mag:75954296","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8382667","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[{"display_name":"Observational Study","id":"https://openalex.org/study-designs/observational-study"}],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W437264","https://openalex.org/W1015601","https://openalex.org/W46797778","https://openalex.org/W69167734","https://openalex.org/W83041967","https://openalex.org/W90602935","https://openalex.org/W178364288","https://openalex.org/W1898710667","https://openalex.org/W1956882355","https://openalex.org/W1964636019","https://openalex.org/W1967446284","https://openalex.org/W1979802144","https://openalex.org/W1997521650","https://openalex.org/W2074260855","https://openalex.org/W2083781121","https://openalex.org/W2096428903","https://openalex.org/W2100088853","https://openalex.org/W2104169484","https://openalex.org/W2104845462","https://openalex.org/W2123428006","https://openalex.org/W2140178603","https://openalex.org/W2142577281","https://openalex.org/W2337814027","https://openalex.org/W2465858525"],"related_works":["https://openalex.org/W7097203788","https://openalex.org/W1497604752","https://openalex.org/W2020092185","https://openalex.org/W1015601","https://openalex.org/W2104169484","https://openalex.org/W2152166138","https://openalex.org/W2001985312","https://openalex.org/W2090284571","https://openalex.org/W2418931842","https://openalex.org/W2100088853"],"abstract_inverted_index":{"PURPOSE:":[0],"The":[1],"purpose":[2],"of":[3,20,35,64,83,100,114,125,153],"this":[4],"study":[5],"was":[6,96],"to":[7,142],"determine":[8],"whether":[9],"herpes":[10],"simplex":[11],"virus":[12],"(HSV)":[13],"transcripts":[14,68,75,109],"are":[15,161],"present":[16,162],"in":[17,53,98,112,118,129,134,151,158],"the":[18,73,88,123,149],"corneas":[19,103,116,137],"patients":[21,31],"with":[22,32,44,148],"chronic":[23],"herpetic":[24],"stromal":[25,36,139],"keratitis.":[26],"METHODS:":[27],"Corneal":[28],"buttons":[29],"from":[30],"a":[33],"history":[34],"keratitis,":[37],"but":[38,106],"no":[39,107],"ongoing":[40],"active":[41],"disease,":[42],"together":[43],"positive":[45],"and":[46],"negative":[47],"control":[48],"tissues,":[49],"were":[50,79,110],"analyzed":[51],"by":[52,104,117,128],"situ":[54,119,130],"hybridization":[55],"using":[56,87],"single-stranded":[57],"RNA":[58],"probes":[59],"for":[60,72,81],"all":[61],"three":[62],"classes":[63],"viral":[65,108],"lytic":[66],"cycle":[67],"as":[69,71],"well":[70],"latency-associated":[74],"(LATs).":[76],"Tissues":[77],"also":[78],"screened":[80],"presence":[82],"HSV":[84,94,132],"genomic":[85],"DNA":[86,95],"polymerase":[89],"chain":[90],"reaction":[91],"(PCR).":[92],"RESULTS:":[93],"detected":[97,111],"7":[99],"13":[101],"quiescent":[102],"PCR,":[105],"any":[113],"these":[115],"hybridization.":[120],"CONCLUSIONS:":[121],"At":[122],"level":[124],"detection":[126],"afforded":[127],"hybridization,":[131],"persistent":[133],"scarred":[135],"human":[136],"after":[138],"keratitis":[140],"appears":[141],"be":[143],"transcriptionally":[144],"dormant.":[145],"This":[146],"contrasts":[147],"situation":[150],"neurons":[152],"latently":[154],"infected":[155],"sensory":[156],"ganglia,":[157],"which":[159],"LATs":[160],"at":[163],"high":[164],"levels.":[165]},"counts_by_year":[{"year":2017,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}