{"id":"https://openalex.org/W755654633","doi":null,"title":"Approaches to purification and generation of monoclonal antibodies of an insulin-sensitive 90 kd protein","display_name":"Approaches to purification and generation of monoclonal antibodies of an insulin-sensitive 90 kd protein","publication_year":1991,"publication_date":"1991-01-01","ids":{"openalex":"https://openalex.org/W755654633","mag":"755654633"},"language":"en","primary_location":{"id":"pmh:oai:repository.arizona.edu:10150/278022","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10150/278022","pdf_url":null,"source":{"id":"https://openalex.org/S4306400271","display_name":"UA Campus Repository (The University of Arizona)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I138006243","host_organization_name":"University of Arizona","host_organization_lineage":["https://openalex.org/I138006243"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Thesis-Reproduction (electronic)"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5091857290","display_name":"Steven Patrick Emmons","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Steven Patrick Emmons","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5091857290"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.4846000075340271,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.4846000075340271,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10839","display_name":"Pancreatic function and diabetes","score":0.13490000367164612,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11339","display_name":"Metabolism, Diabetes, and Cancer","score":0.12349999696016312,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/insulin-receptor","display_name":"insulin receptor","score":0.8360000252723694},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.9039999842643738},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.7009999752044678},{"id":"https://openalex.org/keywords/hybridoma-technology","display_name":"hybridoma technology","score":0.671999990940094},{"id":"https://openalex.org/keywords/electrofusion","display_name":"electrofusion","score":0.6759999990463257},{"id":"https://openalex.org/keywords/lipopolysaccharide","display_name":"lipopolysaccharide","score":0.7039999961853027},{"id":"https://openalex.org/keywords/evolutionary-conservation","display_name":"evolutionary conservation","score":0.5460000038146973},{"id":"https://openalex.org/keywords/insulin-action-mechanism","display_name":"insulin action mechanism","score":0.5690000057220459},{"id":"https://openalex.org/keywords/cross-reactivity","display_name":"cross-reactivity","score":0.8989999890327454}],"concepts":[{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.7660320997238159},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.48735982179641724},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3423168659210205},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24849927425384521},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.20723900198936462}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:repository.arizona.edu:10150/278022","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10150/278022","pdf_url":null,"source":{"id":"https://openalex.org/S4306400271","display_name":"UA Campus Repository (The University of Arizona)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I138006243","host_organization_name":"University of Arizona","host_organization_lineage":["https://openalex.org/I138006243"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Thesis-Reproduction (electronic)"},{"id":"mag:755654633","doi":null,"is_oa":false,"landing_page_url":"http://arizona.openrepository.com/arizona/handle/10150/278022","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":86,"referenced_works":["https://openalex.org/W34265306","https://openalex.org/W54157561","https://openalex.org/W193724875","https://openalex.org/W275146305","https://openalex.org/W276108846","https://openalex.org/W761054824","https://openalex.org/W1040845057","https://openalex.org/W1531997785","https://openalex.org/W1542771188","https://openalex.org/W1551652349","https://openalex.org/W1562713805","https://openalex.org/W1575936827","https://openalex.org/W1591105009","https://openalex.org/W1606423922","https://openalex.org/W1647622717","https://openalex.org/W1685985691","https://openalex.org/W1764805959","https://openalex.org/W1824736693","https://openalex.org/W1900968503","https://openalex.org/W1918840906","https://openalex.org/W1963587176","https://openalex.org/W1969457549","https://openalex.org/W1974869964","https://openalex.org/W1976448511","https://openalex.org/W1977182622","https://openalex.org/W1983122167","https://openalex.org/W1986290104","https://openalex.org/W1988374284","https://openalex.org/W1989237446","https://openalex.org/W1995826320","https://openalex.org/W1999698761","https://openalex.org/W2000322460","https://openalex.org/W2001154029","https://openalex.org/W2004069782","https://openalex.org/W2007020081","https://openalex.org/W2007262483","https://openalex.org/W2014321075","https://openalex.org/W2015657041","https://openalex.org/W2016133020","https://openalex.org/W2018751358","https://openalex.org/W2019724882","https://openalex.org/W2022961230","https://openalex.org/W2023381903","https://openalex.org/W2027421033","https://openalex.org/W2027643789","https://openalex.org/W2029812891","https://openalex.org/W2030962985","https://openalex.org/W2042815000","https://openalex.org/W2044368528","https://openalex.org/W2044936649","https://openalex.org/W2047931302","https://openalex.org/W2053408554","https://openalex.org/W2054408371","https://openalex.org/W2054970879","https://openalex.org/W2057576679","https://openalex.org/W2058019193","https://openalex.org/W2059925951","https://openalex.org/W2064297831","https://openalex.org/W2065889873","https://openalex.org/W2069616351","https://openalex.org/W2073446720","https://openalex.org/W2075188972","https://openalex.org/W2076080073","https://openalex.org/W2078185017","https://openalex.org/W2080221625","https://openalex.org/W2081549369","https://openalex.org/W2082815350","https://openalex.org/W2085118135","https://openalex.org/W2087062084","https://openalex.org/W2095297990","https://openalex.org/W2097996077","https://openalex.org/W2100837269","https://openalex.org/W2101948512","https://openalex.org/W2104918602","https://openalex.org/W2148694525","https://openalex.org/W2149423875","https://openalex.org/W2160222273","https://openalex.org/W2171793923","https://openalex.org/W2176848385","https://openalex.org/W2178538190","https://openalex.org/W2186424937","https://openalex.org/W2239984981","https://openalex.org/W2272723492","https://openalex.org/W2413583915","https://openalex.org/W2462521403","https://openalex.org/W4313310132"],"related_works":["https://openalex.org/W2418537366","https://openalex.org/W2024752670","https://openalex.org/W2055200325","https://openalex.org/W1976139945","https://openalex.org/W2066210780","https://openalex.org/W2111349363","https://openalex.org/W137414433","https://openalex.org/W1479900350","https://openalex.org/W2329583523","https://openalex.org/W2024838196"],"abstract_inverted_index":{"Insulin":[0],"action":[1],"has":[2],"been":[3],"extensively":[4],"studied":[5],"although":[6],"the":[7,12,21,50,93,100,104,107],"exact":[8],"mechanism":[9],"by":[10],"which":[11],"binding":[13],"of":[14,80,106],"this":[15,36,117],"hormone":[16],"to":[17,42,49,92],"its":[18],"receptor":[19],"causes":[20],"observed":[22],"effects":[23],"is":[24],"still":[25],"obsure.":[26],"A":[27],"90":[28,51,94,108],"kd":[29,52,95,109],"membrane":[30],"protein":[31,96,110,118],"may":[32,119,124],"be":[33,120],"involved":[34],"in":[35,84,111,128],"mechanism.":[37],"An":[38],"attempt":[39],"was":[40],"made":[41],"purify":[43],"and":[44,59,86,103,123],"make":[45],"a":[46,60,74,126],"monoclonal":[47,90],"antibody":[48],"protein.":[53],"Several":[54],"purification":[55],"methods":[56,68],"were":[57,69,97],"attempted":[58],"2-D":[61],"electrophoretic":[62],"procedure":[63,77],"developed.":[64],"Conventional":[65],"hybridoma":[66,76],"production":[67],"tried":[70],"as":[71,73],"well":[72],"novel":[75],"using":[78],"selection":[79],"J11Dlo":[81],"cells,":[82],"culture":[83],"LPS/DxSO4,":[85],"electrofusion.":[87],"The":[88],"resulting":[89],"antibodies":[91],"cross-reactive.":[98],"Furthermore,":[99],"immunological":[101],"results":[102],"presence":[105],"several":[112],"mammalian":[113],"species":[114],"suggest":[115],"that":[116],"evolutionarily":[121],"conserved":[122],"play":[125],"role":[127],"insulin":[129],"action.":[130]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
