{"id":"https://openalex.org/W74686033","doi":"https://doi.org/10.1111/j.1470-8744.1996.tb00399.x","title":"Elucidation of N\u2010linked oligosaccharide structures of recombinant human factor VIII using fluorophore\u2010assisted carbohydrate electrophoresis","display_name":"Elucidation of N\u2010linked oligosaccharide structures of recombinant human factor VIII using fluorophore\u2010assisted carbohydrate electrophoresis","publication_year":1996,"publication_date":"1996-12-01","ids":{"openalex":"https://openalex.org/W74686033","doi":"https://doi.org/10.1111/j.1470-8744.1996.tb00399.x","mag":"74686033","pmid":"https://pubmed.ncbi.nlm.nih.gov/8969451"},"language":"en","primary_location":{"id":"doi:10.1111/j.1470-8744.1996.tb00399.x","doi":"https://doi.org/10.1111/j.1470-8744.1996.tb00399.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1470-8744.1996.tb00399.x","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5019081312","display_name":"Harish P. Kumar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99484792","display_name":"Baxter (United States)","ror":"https://ror.org/02d6ew870","country_code":"US","type":"company","lineage":["https://openalex.org/I99484792"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kumar HP","raw_affiliation_strings":["Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA","institution_ids":["https://openalex.org/I99484792"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112856851","display_name":"Chuck Hague","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99484792","display_name":"Baxter (United States)","ror":"https://ror.org/02d6ew870","country_code":"US","type":"company","lineage":["https://openalex.org/I99484792"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C. Hague","raw_affiliation_strings":["Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA","institution_ids":["https://openalex.org/I99484792"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010014668","display_name":"Terry M. Haley","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99484792","display_name":"Baxter (United States)","ror":"https://ror.org/02d6ew870","country_code":"US","type":"company","lineage":["https://openalex.org/I99484792"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T. Haley","raw_affiliation_strings":["Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA","institution_ids":["https://openalex.org/I99484792"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5105450375","display_name":"Starr CM","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99484792","display_name":"Baxter (United States)","ror":"https://ror.org/02d6ew870","country_code":"US","type":"company","lineage":["https://openalex.org/I99484792"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Starr CM","raw_affiliation_strings":["Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA","institution_ids":["https://openalex.org/I99484792"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068055967","display_name":"Marc J. Besman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99484792","display_name":"Baxter (United States)","ror":"https://ror.org/02d6ew870","country_code":"US","type":"company","lineage":["https://openalex.org/I99484792"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Besman MJ","raw_affiliation_strings":["Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA","institution_ids":["https://openalex.org/I99484792"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111532994","display_name":"Roger L. Lundblad","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99484792","display_name":"Baxter (United States)","ror":"https://ror.org/02d6ew870","country_code":"US","type":"company","lineage":["https://openalex.org/I99484792"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lundblad RL","raw_affiliation_strings":["Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA","institution_ids":["https://openalex.org/I99484792"]}]},{"author_position":"last","author":{"id":null,"display_name":"D. Baker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I99484792","display_name":"Baxter (United States)","ror":"https://ror.org/02d6ew870","country_code":"US","type":"company","lineage":["https://openalex.org/I99484792"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D. Baker","raw_affiliation_strings":["Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Baxter Biotech, Hyland R&D Division, Duarte, CA 91010, USA","institution_ids":["https://openalex.org/I99484792"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I99484792"],"apc_list":{"value":3470,"currency":"USD","value_usd":3470},"apc_paid":null,"fwci":0.8836,"has_fulltext":false,"cited_by_count":33,"citation_normalized_percentile":{"value":0.70840447,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"24","issue":"3","first_page":"207","last_page":"216"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9682999849319458,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9682999849319458,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.021700000390410423,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11280","display_name":"Hemophilia Treatment and Research","score":0.003800000064074993,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/n-glycans","display_name":"N-glycans","score":0.9700000286102295},{"id":"https://openalex.org/keywords/glycosylation","display_name":"glycosylation","score":0.6489999890327454},{"id":"https://openalex.org/keywords/cho-cell-expression","display_name":"CHO cell expression","score":0.5600000023841858},{"id":"https://openalex.org/keywords/sialic-acid","display_name":"sialic acid","score":0.6589999794960022},{"id":"https://openalex.org/keywords/core-fucosylation","display_name":"core fucosylation","score":0.7820000052452087},{"id":"https://openalex.org/keywords/oligosaccharide-structure-elucidation","display_name":"oligosaccharide structure elucidation","score":0.6809999942779541},{"id":"https://openalex.org/keywords/hemophilia-a","display_name":"hemophilia A","score":0.7120000123977661},{"id":"https://openalex.org/keywords/process-consistency","display_name":"process consistency","score":0.5669999718666077},{"id":"https://openalex.org/keywords/glycoprotein-characterization","display_name":"glycoprotein characterization","score":0.5170000195503235}],"concepts":[{"id":"https://openalex.org/C2777726330","wikidata":"https://www.wikidata.org/wiki/Q320607","display_name":"Oligosaccharide","level":2,"score":0.8874577283859253},{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.7872678637504578},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7248458862304688},{"id":"https://openalex.org/C2780654058","wikidata":"https://www.wikidata.org/wiki/Q5376339","display_name":"Endoglycosidase","level":3,"score":0.6868171691894531},{"id":"https://openalex.org/C108625454","wikidata":"https://www.wikidata.org/wiki/Q187126","display_name":"Glycoprotein","level":2,"score":0.6152549386024475},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6150505542755127},{"id":"https://openalex.org/C2777313579","wikidata":"https://www.wikidata.org/wiki/Q898365","display_name":"Glycosylation","level":2,"score":0.5633934140205383},{"id":"https://openalex.org/C2781178804","wikidata":"https://www.wikidata.org/wiki/Q5420588","display_name":"Exoglycosidase","level":4,"score":0.5295143127441406},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.4790812134742737},{"id":"https://openalex.org/C79156339","wikidata":"https://www.wikidata.org/wiki/Q5037878","display_name":"Carbohydrate conformation","level":3,"score":0.4222587049007416},{"id":"https://openalex.org/C206212055","wikidata":"https://www.wikidata.org/wiki/Q2553138","display_name":"Glycan","level":3,"score":0.4045276641845703},{"id":"https://openalex.org/C100817775","wikidata":"https://www.wikidata.org/wiki/Q134219","display_name":"Polysaccharide","level":2,"score":0.12696096301078796},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10883629322052002},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002236","descriptor_name":"Carbohydrate Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002240","descriptor_name":"Carbohydrate Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004586","descriptor_name":"Electrophoresis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D005169","descriptor_name":"Factor VIII","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D005169","descriptor_name":"Factor VIII","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005169","descriptor_name":"Factor VIII","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006596","descriptor_name":"Hexosaminidases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009844","descriptor_name":"Oligosaccharides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1111/j.1470-8744.1996.tb00399.x","doi":"https://doi.org/10.1111/j.1470-8744.1996.tb00399.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1470-8744.1996.tb00399.x","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},{"id":"pmid:8969451","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8969451","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and applied biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1637694297","https://openalex.org/W2058828936","https://openalex.org/W7006061331","https://openalex.org/W1589732206","https://openalex.org/W1990812932","https://openalex.org/W2808009529","https://openalex.org/W2048402225","https://openalex.org/W2103858837","https://openalex.org/W2148983374","https://openalex.org/W781727592"],"abstract_inverted_index":{"Characterization":[0],"of":[1,9,13,36,100,124,129,141,152,222,225,235,241],"the":[2,34,49,54,74,121,125,139,142,153,172,210],"carbohydrate":[3,81],"moiety":[4],"is":[5,30],"a":[6,25],"critical":[7],"measure":[8],"manufacturing":[10],"process":[11],"consistency":[12],"recombinant":[14],"human":[15],"Factor":[16],"VIII":[17],"(rFVIII)":[18],"in":[19,46,158,168,203,209,218],"Chinese-hamster":[20],"ovary":[21],"(CHO)":[22],"cells.":[23],"FVIII,":[24],"large":[26],"(300":[27],"kDa)":[28],"glycoprotein,":[29],"employed":[31],"therapeutically":[32],"for":[33],"correction":[35],"haemophilia":[37],"A.":[38],"While":[39],"N-linked":[40,55,58],"and":[41,77,91,102,132,138,186],"O-linked":[42],"oligosaccharides":[43,59,143],"are":[44,175],"found":[45,147,157,208],"this":[47],"protein,":[48],"current":[50],"study":[51],"focuses":[52],"on":[53,104,115,230],"oligosaccharides.":[56,126,244],"The":[57,84,108,194,213],"from":[60],"rFVIII":[61,160,205,223],"were":[62,89,112,136,146,206,216],"released":[63],"using":[64],"either":[65],"peptide":[66],"N-glycosidase":[67],"F":[68],"or":[69],"endoglycosidase":[70],"H,":[71],"derivatized":[72],"with":[73,97],"fluorophore":[75],"8-aminonaphthalene-1,3,6-trisulphonate,":[76],"analysed":[78],"by":[79,182],"fluorophore-assisted":[80],"electrophoresis":[82],"(FACE).":[83],"electrophoretically":[85],"resolved":[86],"oligosaccharide":[87,155],"bands":[88,93,236],"isolated":[90],"individual":[92],"subjected":[94],"to":[95,119,148,165,200],"digestion":[96],"defined":[98],"pools":[99],"exoglycosidases":[101],"re-electrophoresed":[103],"FACE":[105],"sequencing":[106],"gels.":[107],"resulting":[109],"gel":[110],"patterns":[111,215],"interpreted,":[113],"based":[114,229],"band":[116],"mobility":[117],"shifts,":[118],"obtain":[120],"sequence":[122],"structure":[123],"A":[127],"total":[128],"eight":[130],"acidic":[131],"12":[133],"neutral":[134],"structures":[135,156,190,198],"identified,":[137],"majority":[140],"(approximately":[144,179],"92%)":[145],"be":[149,166,201],"sialylated.":[150],"All":[151],"major":[154],"CHO-cell-derived":[159,211],"have":[161],"also":[162],"been":[163],"reported":[164,199],"present":[167,202],"plasma-derived":[169],"FVIII.":[170],"Among":[171],"most":[173],"abundant":[174],"disialylated,":[176],"biantennary,":[177,188],"core-fucosylated":[178,185,189],"40%),":[180],"followed":[181],"trisialylated,":[183],"triantennary,":[184],"monosialo,":[187],"(each":[191],"approximately":[192,239],"18%).":[193],"Gal":[195],"alpha":[196],"1-3Gal":[197],"baby-hamster-kidney-cell-derived":[204],"not":[207],"protein.":[212],"glycosylation":[214],"consistent":[217],"six":[219],"random":[220],"lots":[221],"[coefficient":[224],"variation":[226],"(%)":[227],"3-14]":[228],"percentage":[231],"lane":[232],"luminance":[233],"data":[234],"that":[237],"represent":[238],"98%":[240],"all":[242],"asparagine-linked":[243]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}