{"id":"https://openalex.org/W73847090","doi":null,"title":"Create the Scene and Watch the Show Unfold: Following Vegetative to Embryonic Developmental Transitions by Over-Expressing LEC2 in Leaves of Arabidopsis thaliana","display_name":"Create the Scene and Watch the Show Unfold: Following Vegetative to Embryonic Developmental Transitions by Over-Expressing LEC2 in Leaves of Arabidopsis thaliana","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W73847090","mag":"73847090"},"language":"en","primary_location":{"id":"pmh:oai:ir.lib.uwo.ca:etd-1806","doi":null,"is_oa":true,"landing_page_url":"https://ir.lib.uwo.ca/etd/600","pdf_url":"https://ir.lib.uwo.ca/etd/600","source":{"id":"https://openalex.org/S4306400648","display_name":"Scholarship@Western (Western University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I125749732","host_organization_name":"Western University","host_organization_lineage":["https://openalex.org/I125749732"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Thesis and Dissertation Repository","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://ir.lib.uwo.ca/etd/600","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080865130","display_name":"Mistianne Feeney","orcid":"https://orcid.org/0000-0002-0057-7753","observed_orcids":["https://orcid.org/0000-0002-0057-7753"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Mistianne Feeney","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-0057-7753","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5080865130"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00959219,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.5356000065803528,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.5356000065803528,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11098","display_name":"Seed Germination and Physiology","score":0.15189999341964722,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.10239999741315842,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lec2","display_name":"LEC2","score":0.8410000205039978},{"id":"https://openalex.org/keywords/protein-storage-vacuoles","display_name":"protein storage vacuoles","score":0.7910000085830688},{"id":"https://openalex.org/keywords/lytic-vacuole","display_name":"lytic vacuole","score":0.9879999756813049},{"id":"https://openalex.org/keywords/vacuole-biogenesis","display_name":"vacuole biogenesis","score":0.8019999861717224},{"id":"https://openalex.org/keywords/leaf-development","display_name":"leaf development","score":0.4950000047683716},{"id":"https://openalex.org/keywords/embryonic-development","display_name":"embryonic development","score":0.5220000147819519},{"id":"https://openalex.org/keywords/seed-storage-proteins","display_name":"seed storage proteins","score":0.8629999756813049},{"id":"https://openalex.org/keywords/tonoplast-intrinsic-proteins","display_name":"tonoplast intrinsic proteins","score":0.8050000071525574},{"id":"https://openalex.org/keywords/oil-bodies","display_name":"oil bodies","score":0.8989999890327454},{"id":"https://openalex.org/keywords/arabidopsis-thaliana","display_name":"Arabidopsis thaliana","score":0.8289999961853027},{"id":"https://openalex.org/keywords/dexamethasone-induction","display_name":"dexamethasone induction","score":0.7749999761581421}],"concepts":[{"id":"https://openalex.org/C2775988993","wikidata":"https://www.wikidata.org/wiki/Q158695","display_name":"Arabidopsis thaliana","level":4,"score":0.6782851219177246},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4975612461566925},{"id":"https://openalex.org/C2779491563","wikidata":"https://www.wikidata.org/wiki/Q157892","display_name":"Arabidopsis","level":4,"score":0.42413759231567383},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.4188605546951294},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3373359143733978},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13037031888961792},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11240500211715698},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:ir.lib.uwo.ca:etd-1806","doi":null,"is_oa":true,"landing_page_url":"https://ir.lib.uwo.ca/etd/600","pdf_url":"https://ir.lib.uwo.ca/etd/600","source":{"id":"https://openalex.org/S4306400648","display_name":"Scholarship@Western (Western University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I125749732","host_organization_name":"Western University","host_organization_lineage":["https://openalex.org/I125749732"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Thesis and Dissertation Repository","raw_type":"text"},{"id":"pmh:oai:uwo.scholaris.ca:20.500.14721/27081","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/20.500.14721/27081","pdf_url":null,"source":{"id":"https://openalex.org/S4306400648","display_name":"Scholarship@Western (Western University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I125749732","host_organization_name":"Western University","host_organization_lineage":["https://openalex.org/I125749732"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"thesis"},{"id":"mag:73847090","doi":null,"is_oa":false,"landing_page_url":"https://ir.lib.uwo.ca/cgi/viewcontent.cgi?article=1806&context=etd","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:ir.lib.uwo.ca:etd-1806","doi":null,"is_oa":true,"landing_page_url":"https://ir.lib.uwo.ca/etd/600","pdf_url":"https://ir.lib.uwo.ca/etd/600","source":{"id":"https://openalex.org/S4306400648","display_name":"Scholarship@Western (Western University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I125749732","host_organization_name":"Western University","host_organization_lineage":["https://openalex.org/I125749732"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Thesis and Dissertation Repository","raw_type":"text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322551","display_name":"Simon Fraser University","ror":"https://ror.org/0213rcc28"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W73847090.pdf","grobid_xml":"https://content.openalex.org/works/W73847090.grobid-xml"},"referenced_works_count":244,"referenced_works":["https://openalex.org/W30055986","https://openalex.org/W165626112","https://openalex.org/W219014748","https://openalex.org/W846410266","https://openalex.org/W1250252024","https://openalex.org/W1503466990","https://openalex.org/W1514420941","https://openalex.org/W1543329883","https://openalex.org/W1550512525","https://openalex.org/W1590397189","https://openalex.org/W1592881218","https://openalex.org/W1595842211","https://openalex.org/W1596213165","https://openalex.org/W1719075847","https://openalex.org/W1757147296","https://openalex.org/W1763142230","https://openalex.org/W1775749144","https://openalex.org/W1901316837","https://openalex.org/W1919461715","https://openalex.org/W1955382403","https://openalex.org/W1963904531","https://openalex.org/W1965939781","https://openalex.org/W1966232294","https://openalex.org/W1966543767","https://openalex.org/W1966697893","https://openalex.org/W1967699966","https://openalex.org/W1967715486","https://openalex.org/W1969501907","https://openalex.org/W1970853249","https://openalex.org/W1971841127","https://openalex.org/W1971910567","https://openalex.org/W1972132893","https://openalex.org/W1974813506","https://openalex.org/W1974841051","https://openalex.org/W1976090083","https://openalex.org/W1976253033","https://openalex.org/W1977437112","https://openalex.org/W1980168309","https://openalex.org/W1981259895","https://openalex.org/W1983683478","https://openalex.org/W1984315509","https://openalex.org/W1985023202","https://openalex.org/W1985651601","https://openalex.org/W1992638797","https://openalex.org/W1992670818","https://openalex.org/W1993530554","https://openalex.org/W1995035813","https://openalex.org/W1995541004","https://openalex.org/W1997203740","https://openalex.org/W1999466350","https://openalex.org/W1999477136","https://openalex.org/W2000827842","https://openalex.org/W2001591494","https://openalex.org/W2005693181","https://openalex.org/W2005889564","https://openalex.org/W2007085385","https://openalex.org/W2008149538","https://openalex.org/W2010055122","https://openalex.org/W2010614830","https://openalex.org/W2012780693","https://openalex.org/W2014529027","https://openalex.org/W2015946583","https://openalex.org/W2017915640","https://openalex.org/W2018155121","https://openalex.org/W2018702925","https://openalex.org/W2018813278","https://openalex.org/W2019371211","https://openalex.org/W2024225611","https://openalex.org/W2025017585","https://openalex.org/W2025935801","https://openalex.org/W2026269367","https://openalex.org/W2026809645","https://openalex.org/W2026895463","https://openalex.org/W2027958182","https://openalex.org/W2027961934","https://openalex.org/W2029088142","https://openalex.org/W2029329199","https://openalex.org/W2029932852","https://openalex.org/W2030780557","https://openalex.org/W2031126873","https://openalex.org/W2031602635","https://openalex.org/W2031761299","https://openalex.org/W2031792440","https://openalex.org/W2033322678","https://openalex.org/W2035874951","https://openalex.org/W2039022003","https://openalex.org/W2039498507","https://openalex.org/W2043247882","https://openalex.org/W2044676789","https://openalex.org/W2045863873","https://openalex.org/W2046837813","https://openalex.org/W2047584139","https://openalex.org/W2051240337","https://openalex.org/W2051809332","https://openalex.org/W2052970640","https://openalex.org/W2054614363","https://openalex.org/W2057608504","https://openalex.org/W2058465353","https://openalex.org/W2059866766","https://openalex.org/W2060005410","https://openalex.org/W2061230001","https://openalex.org/W2061713599","https://openalex.org/W2066228716","https://openalex.org/W2067414568","https://openalex.org/W2067686807","https://openalex.org/W2069097240","https://openalex.org/W2069671139","https://openalex.org/W2071030483","https://openalex.org/W2072210213","https://openalex.org/W2074284227","https://openalex.org/W2075107354","https://openalex.org/W2075925032","https://openalex.org/W2076947759","https://openalex.org/W2077271398","https://openalex.org/W2079449882","https://openalex.org/W2079503848","https://openalex.org/W2080089217","https://openalex.org/W2080363541","https://openalex.org/W2082679628","https://openalex.org/W2086443637","https://openalex.org/W2086485726","https://openalex.org/W2088759895","https://openalex.org/W2089919205","https://openalex.org/W2091554063","https://openalex.org/W2092500742","https://openalex.org/W2096053032","https://openalex.org/W2096129129","https://openalex.org/W2096365445","https://openalex.org/W2098316195","https://openalex.org/W2098769040","https://openalex.org/W2099436557","https://openalex.org/W2100874251","https://openalex.org/W2103215727","https://openalex.org/W2103525149","https://openalex.org/W2103793524","https://openalex.org/W2103973399","https://openalex.org/W2104196253","https://openalex.org/W2104636880","https://openalex.org/W2105259668","https://openalex.org/W2105868453","https://openalex.org/W2106041972","https://openalex.org/W2106190285","https://openalex.org/W2106852876","https://openalex.org/W2107127679","https://openalex.org/W2107244388","https://openalex.org/W2107366172","https://openalex.org/W2108960783","https://openalex.org/W2108979713","https://openalex.org/W2109230943","https://openalex.org/W2109259920","https://openalex.org/W2109604687","https://openalex.org/W2111386845","https://openalex.org/W2111832298","https://openalex.org/W2113968207","https://openalex.org/W2114182170","https://openalex.org/W2114619977","https://openalex.org/W2115094992","https://openalex.org/W2115420459","https://openalex.org/W2115456678","https://openalex.org/W2115779663","https://openalex.org/W2117760086","https://openalex.org/W2120521842","https://openalex.org/W2121713614","https://openalex.org/W2123775894","https://openalex.org/W2124301540","https://openalex.org/W2124815236","https://openalex.org/W2125989300","https://openalex.org/W2126306449","https://openalex.org/W2126707901","https://openalex.org/W2127632851","https://openalex.org/W2127816774","https://openalex.org/W2128097362","https://openalex.org/W2128635872","https://openalex.org/W2128860085","https://openalex.org/W2129323234","https://openalex.org/W2129462035","https://openalex.org/W2129997424","https://openalex.org/W2130132005","https://openalex.org/W2130148186","https://openalex.org/W2130228991","https://openalex.org/W2130913636","https://openalex.org/W2131462739","https://openalex.org/W2132421343","https://openalex.org/W2132626957","https://openalex.org/W2135310874","https://openalex.org/W2136320374","https://openalex.org/W2137439541","https://openalex.org/W2137709522","https://openalex.org/W2138776980","https://openalex.org/W2140007950","https://openalex.org/W2140905053","https://openalex.org/W2140954431","https://openalex.org/W2141207650","https://openalex.org/W2141898637","https://openalex.org/W2141985540","https://openalex.org/W2142389038","https://openalex.org/W2143292221","https://openalex.org/W2143670987","https://openalex.org/W2144272594","https://openalex.org/W2144466133","https://openalex.org/W2146563540","https://openalex.org/W2147588538","https://openalex.org/W2148712475","https://openalex.org/W2148721211","https://openalex.org/W2150058998","https://openalex.org/W2151001790","https://openalex.org/W2152451823","https://openalex.org/W2152862254","https://openalex.org/W2153665389","https://openalex.org/W2153677263","https://openalex.org/W2154873669","https://openalex.org/W2155227945","https://openalex.org/W2156172229","https://openalex.org/W2158772921","https://openalex.org/W2158891619","https://openalex.org/W2159230907","https://openalex.org/W2161745940","https://openalex.org/W2161764757","https://openalex.org/W2161910309","https://openalex.org/W2162015038","https://openalex.org/W2162077247","https://openalex.org/W2162526240","https://openalex.org/W2163028631","https://openalex.org/W2163975773","https://openalex.org/W2166967975","https://openalex.org/W2167977177","https://openalex.org/W2168312236","https://openalex.org/W2168728371","https://openalex.org/W2168894046","https://openalex.org/W2169336947","https://openalex.org/W2169358467","https://openalex.org/W2169848985","https://openalex.org/W2170208222","https://openalex.org/W2170641683","https://openalex.org/W2171257497","https://openalex.org/W2171339738","https://openalex.org/W2172659117","https://openalex.org/W2176603593","https://openalex.org/W2245156847","https://openalex.org/W2336000185","https://openalex.org/W2336867229","https://openalex.org/W2412521999","https://openalex.org/W4243399007","https://openalex.org/W4250216037"],"related_works":["https://openalex.org/W2078574717","https://openalex.org/W2093947711","https://openalex.org/W4254629656","https://openalex.org/W2792519735","https://openalex.org/W6986671632","https://openalex.org/W2018813278","https://openalex.org/W7100737945","https://openalex.org/W2135310874","https://openalex.org/W2158891619","https://openalex.org/W2029329199"],"abstract_inverted_index":{"During":[0],"seed":[1,19,24,150,175,231],"development,":[2,176],"the":[3,31,38,60,85,127,131,170,201,214,225,244,267,301,307,311],"lytic":[4],"vacuole":[5,13,63,76,270,313],"(LV)":[6],"is":[7,33,94,166,314,330],"replaced":[8,36,156,315],"by":[9,37,157,316],"a":[10,95,103,263,293,305,331,341],"protein":[11,25,162,211],"storage":[12,20],"(PSV)":[14],"which":[15,40,147],"specializes":[16],"in":[17,45,72,78,181,196,233,323,325],"accumulating":[18],"proteins":[21,232],"(SSPs).":[22],"As":[23],"reserves":[26],"are":[27,55,148],"mobilized":[28],"upon":[29],"germination,":[30],"PSV":[32],"once":[34],"again":[35],"LV":[39,154,294,302],"takes":[41],"on":[42,254],"different":[43],"roles":[44],"vegetative":[46,114],"tissues.":[47],"Cellular":[48],"events":[49],"occurring":[50],"during":[51,174],"these":[52],"developmental":[53,119,202],"transitions":[54,77,303],"not":[56],"well":[57],"understood,":[58],"particularly,":[59],"transition":[61,268],"between":[62,269],"types.":[64],"This":[65],"research":[66],"investigates":[67],"whether":[68],"PSVs":[69,322],"can":[70],"exist":[71],"leaves.":[73,182],"To":[74],"study":[75,335],"leaves,":[79],"an":[80,122,290],"Arabidopsis":[81,185],"thaliana":[82],"line":[83],"over-expressing":[84],"LEAFY":[86],"COTYLEDON2":[87],"(LEC2)":[88],"transcription":[89],"factor":[90],"was":[91,155,179,272,286],"used.":[92],"LEC2":[93,112,125,165,238,328],"master":[96],"regulator":[97],"of":[98,111,172,207,230,237,246,266,283,292,321,344],"embryogenesis":[99],"responsible":[100,167],"for":[101,168],"creating":[102],"cellular":[104,226],"environment":[105],"that":[106,160,299],"promotes":[107],"embryogenic":[108],"development.":[109],"Over-expression":[110],"causes":[113],"tissues":[115],"to":[116,121,190,278,288,304,327,334,340],"change":[117],"their":[118,345],"fate":[120],"embryonic":[123,247],"state.":[124],"alters":[126],"leaf":[128,197,284],"phenotype":[129],"at":[130],"subcellular":[132],"level;":[133],"chloroplasts":[134],"de-differentiated":[135],"and":[136,203,228,256,295,337],"contained":[137],"more":[138,258],"starch.":[139],"The":[140,152,183,281,319],"cytoplasm":[141],"becomes":[142],"filled":[143],"with":[144,240],"oil":[145],"bodies,":[146],"typically":[149],"organelles.":[151],"large":[153,312],"small-sized":[158],"vacuoles":[159,195,216,285,336],"accumulated":[161],"deposits.":[163],"Since":[164],"activating":[169],"synthesis":[171],"SSPs":[173],"SSP":[177,186],"accumulation":[178,229],"investigated":[180],"major":[184],"families":[187],"were":[188,217],"shown":[189],"accumulate":[191],"within":[192],"small":[193,215],"sized":[194],"cells.":[198],"By":[199],"exploiting":[200],"tissue":[204],"specific":[205],"localization":[206],"two":[208],"tonoplast":[209,308],"intrinsic":[210],"(TIP)":[212],"isoforms,":[213],"identified":[218],"as":[219,249,251,300],"PSVs.":[220,318],"A":[221],"time":[222],"course":[223],"following":[224],"alterations":[227],"leaves":[234,324],"after":[235],"induction":[236],"activity":[239],"dexamethasone":[241],"(DEX)":[242],"revealed":[243],"appearance":[245],"characteristics":[248],"early":[250],"4":[252],"days":[253],"DEX":[255],"became":[257],"prominent":[259],"over":[260],"time.":[261],"Additionally,":[262],"dynamic":[264],"view":[265],"types":[271],"observed":[273],"using":[274],"TIP":[275],"isoforms":[276],"fused":[277],"fluorescent":[279],"markers.":[280],"morphology":[282],"altered":[287],"resemble":[289],"amalgamation":[291],"PSV.":[296],"Results":[297],"suggest":[298],"PSV,":[306],"remodels":[309],"before":[310],"smaller":[317],"formation":[320],"response":[326],"over-expression":[329],"novel":[332],"approach":[333],"will":[338],"lead":[339],"better":[342],"understanding":[343],"basic":[346],"biology.":[347]},"counts_by_year":[],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}