{"id":"https://openalex.org/W73117190","doi":null,"title":"In vitro maintenance and retroviral transduction of human myeloma cells in long-term marrow cultures.","display_name":"In vitro maintenance and retroviral transduction of human myeloma cells in long-term marrow cultures.","publication_year":1997,"publication_date":"1997-05-01","ids":{"openalex":"https://openalex.org/W73117190","mag":"73117190","pmid":"https://pubmed.ncbi.nlm.nih.gov/9171933"},"language":"en","primary_location":{"id":"pmid:9171933","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9171933","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer gene therapy","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112260716","display_name":"A. Keith Stewart","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A K Stewart","raw_affiliation_strings":["Toronto Hospital Oncology Gene Therapy Program, Ontario, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Toronto Hospital Oncology Gene Therapy Program, Ontario, Canada","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031193094","display_name":"Henry Miles Prince","orcid":"https://orcid.org/0000-0002-0058-2448","observed_orcids":["https://orcid.org/0000-0002-0058-2448"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H M Prince","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015780974","display_name":"D Cappe","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D Cappe","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112092724","display_name":"Peter T.Y. Chu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P Chu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021982985","display_name":"Carolyn Lutzko","orcid":"https://orcid.org/0009-0001-6266-3470","observed_orcids":["https://orcid.org/0009-0001-6266-3470"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C Lutzko","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064109004","display_name":"D. Robert Sutherland","orcid":"https://orcid.org/0000-0003-1483-5546","observed_orcids":["https://orcid.org/0000-0003-1483-5546"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D R Sutherland","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5012330078","display_name":"Ian D. Dub\u00e9","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"I D Dub\u00e9","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.7689,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.69450962,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"4","issue":"3","first_page":"148","last_page":"56"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10649","display_name":"Multiple Myeloma Research and Treatments","score":0.8877000212669373,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10649","display_name":"Multiple Myeloma Research and Treatments","score":0.8877000212669373,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.0908999964594841,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.009999999776482582,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/multiple-myeloma","display_name":"multiple myeloma","score":0.9980000257492065},{"id":"https://openalex.org/keywords/retroviral-transduction","display_name":"retroviral transduction","score":0.8349999785423279},{"id":"https://openalex.org/keywords/long-term-marrow-culture","display_name":"long-term marrow culture","score":0.9380000233650208},{"id":"https://openalex.org/keywords/gene-marking","display_name":"gene marking","score":0.6769999861717224},{"id":"https://openalex.org/keywords/stem-cell-transplantation","display_name":"stem cell transplantation","score":0.7129999995231628},{"id":"https://openalex.org/keywords/plasma-cells","display_name":"plasma cells","score":0.5590000152587891},{"id":"https://openalex.org/keywords/g418-resistance","display_name":"G418 resistance","score":0.640999972820282},{"id":"https://openalex.org/keywords/lacz-reporter","display_name":"lacZ reporter","score":0.843999981880188},{"id":"https://openalex.org/keywords/clonality-analysis","display_name":"clonality analysis","score":0.41600000858306885}],"concepts":[{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.6893825531005859},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.6662936806678772},{"id":"https://openalex.org/C15152581","wikidata":"https://www.wikidata.org/wiki/Q7833966","display_name":"Transduction (biophysics)","level":2,"score":0.6226170659065247},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6025118231773376},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.594622015953064},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.5723971724510193},{"id":"https://openalex.org/C2776364478","wikidata":"https://www.wikidata.org/wiki/Q467635","display_name":"Multiple myeloma","level":2,"score":0.5480714440345764},{"id":"https://openalex.org/C2780007613","wikidata":"https://www.wikidata.org/wiki/Q546523","display_name":"Bone marrow","level":2,"score":0.5324184894561768},{"id":"https://openalex.org/C26291073","wikidata":"https://www.wikidata.org/wiki/Q310484","display_name":"Ex vivo","level":3,"score":0.4751589000225067},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.46818840503692627},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4670355021953583},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4505116641521454},{"id":"https://openalex.org/C131665280","wikidata":"https://www.wikidata.org/wiki/Q14866169","display_name":"Interleukin 3","level":5,"score":0.42353254556655884},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.33220165967941284},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.329531192779541},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.27952340245246887},{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.17042776942253113},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.14619576930999756},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09823623299598694},{"id":"https://openalex.org/C129374314","wikidata":"https://www.wikidata.org/wiki/Q909381","display_name":"Interleukin 21","level":4,"score":0.08952662348747253},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001854","descriptor_name":"Bone Marrow Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005069","descriptor_name":"Evaluation Studies as Topic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005819","descriptor_name":"Genetic Markers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008558","descriptor_name":"Melphalan","qualifier_ui":"Q000627","qualifier_name":"therapeutic use","is_major_topic":false},{"descriptor_ui":"D008979","descriptor_name":"Moloney murine leukemia virus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009101","descriptor_name":"Multiple Myeloma","qualifier_ui":"Q000188","qualifier_name":"drug therapy","is_major_topic":false},{"descriptor_ui":"D009101","descriptor_name":"Multiple Myeloma","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009101","descriptor_name":"Multiple Myeloma","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D010950","descriptor_name":"Plasma Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018906","descriptor_name":"Antineoplastic Agents, Alkylating","qualifier_ui":"Q000627","qualifier_name":"therapeutic use","is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:9171933","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9171933","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer gene therapy","raw_type":"Journal Article"},{"id":"mag:73117190","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9171933","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.98580002784729,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2031403346","https://openalex.org/W2089470421","https://openalex.org/W2149421297","https://openalex.org/W2305289606","https://openalex.org/W2058157131","https://openalex.org/W1980717441","https://openalex.org/W215259619","https://openalex.org/W2152197624","https://openalex.org/W16036224","https://openalex.org/W2412590741"],"abstract_inverted_index":{"One":[0],"objective":[1],"of":[2,27,43,63,96,103,120,138,149,163,165,174,230,240,252,266,275,280,296,342,348,366,397],"clinical":[3,395],"gene":[4,61,77,91,215,243,400],"marking":[5,42,62],"trials":[6,396],"in":[7,35,47,67,105,218,225,245,285,369,376,394],"multiple":[8,110],"myeloma":[9,31,65,271,304,345,367],"(MM)":[10],"is":[11,23,38],"to":[12,16,25,55,89,373],"determine":[13],"the":[14,28,150,155,172,190,228,231,238,264,270,276,303,343,364],"extent":[15],"which":[17,49],"relapse":[18],"after":[19,117],"stem":[20,100],"cell":[21,209,212,353,399],"transplant":[22],"attributable":[24],"contamination":[26,210],"autograft":[29],"with":[30,73,322],"cells.":[32,101],"A":[33],"requirement":[34],"these":[36,382],"studies":[37],"ex":[39],"vivo":[40],"genetic":[41],"malignant":[44,122,208],"cells":[45,66,104,123,224,282,321,368,383],"present":[46],"autografts":[48],"are":[50],"derived":[51],"from":[52,70,250,350],"patients":[53,72,221,255],"exposed":[54],"significant":[56],"prior":[57,204],"chemotherapy.":[58],"We":[59],"evaluated":[60],"cloonogenic":[64],"marrow":[68,83],"aspirates":[69],"14":[71,277],"MM.":[74],"To":[75],"effect":[76],"transfer":[78,92,216,295],"we":[79],"utilized":[80],"a":[81,94,147,338],"long-term":[82],"culture":[84,121,233],"(LTMC)":[85],"system":[86],"previously":[87],"shown":[88],"facilitate":[90],"into":[93,302],"spectrum":[95],"hematopoietic":[97,167,398],"progenitor":[98],"and":[99,116,130,184,195,300,315,325],"Transduction":[102],"LTMC":[106,114,141],"was":[107,153,187,200,283,291,359],"performed":[108],"by":[109,126,171,180],"supernatant":[111],"exposure.":[112],"At":[113],"initiation":[115],"21":[118,140,247,374],"days":[119,375],"were":[124],"assessed":[125],"morphology,":[127],"flow":[128],"cytometry,":[129],"polymerase":[131],"chain":[132,327],"reaction":[133],"(PCR).":[134],"The":[135,161],"mean":[136],"number":[137,173],"day":[139,246],"adherent":[142],"layer-derived":[143],"granulocyte/macrophage":[144],"progenitors":[145,168],"as":[146,169],"percentage":[148],"original":[151],"inoculum":[152],"within":[154,189],"normal":[156,166],"range":[157],"for":[158,177,227,269,294,313,335,371],"this":[159],"technique.":[160],"efficiency":[162,217],"transduction":[164,389],"determined":[170],"colonies":[175,309,333,354],"positive":[176,334],"proviral":[178],"DNA":[179,249,349],"PCR,":[181],"G418":[182],"resistance,":[183],"X-gal":[185],"staining":[186,312],"also":[188],"expected":[191],"range;":[192],"65%,":[193],"44%":[194],"23%,":[196],"respectively.":[197],"Thus,":[198],"there":[199,290],"no":[201],"evidence":[202,293],"that":[203,381],"chemotherapy":[205],"exposure":[206],"or":[207,214],"compromised":[211],"survival":[213],"LTMC.":[219,286,377],"All":[220],"retained":[222],"plasma":[223,320,352],"LTMCs":[226],"duration":[229],"21-day":[232],"period.":[234],"Molecular":[235],"analysis":[236],"confirmed":[237,263],"persistence":[239],"clonal":[241],"IgVH":[242,267],"rearrangements":[244,268],"LTMC-derived":[248],"6":[251],"12":[253],"informative":[254],"(50%).":[256],"PCR":[257,347],"using":[258,355,388],"allele-specific":[259],"primers":[260,358],"when":[261],"available":[262],"specificity":[265],"clone.":[272,346],"In":[273,287,329],"2":[274],"patients,":[278],"expansion":[279],"clonogenic":[281],"demonstrated":[284,310],"both":[288],"cases":[289],"strong":[292],"reporter":[297],"genes":[298],"(neo":[299],"LacZ)":[301],"clone:":[305],"morphologically":[306],"abnormal":[307],"G418-resistant":[308],"intense":[311],"beta-galactosidase,":[314],"cytospin":[316],"preparations":[317],"showed":[318],"100%":[319],"monoclonal":[323],"heavy":[324],"light":[326],"restriction.":[328],"one":[330],"patient,":[331],"individual":[332],"beta-galactosidase":[336],"bore":[337],"cytogenetic":[339],"abnormality":[340],"characteristic":[341],"patient's":[344],"pooled":[351],"tumor-specific":[356],"CDR3":[357],"positive.":[360],"Our":[361],"results":[362],"demonstrate":[363],"maintenance":[365],"vitro":[370],"up":[372],"They":[378],"further":[379],"illustrate":[380],"can":[384],"be":[385],"genetically":[386],"marked":[387],"protocols":[390],"currently":[391],"being":[392],"tested":[393],"transfer.":[401]},"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
