{"id":"https://openalex.org/W72954606","doi":null,"title":"Fidelity of Plasmodium falciparum apicoplast DNA polymerase","display_name":"Fidelity of Plasmodium falciparum apicoplast DNA polymerase","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W72954606","mag":"72954606"},"language":"en","primary_location":{"id":"pmh:oai:lib.dr.iastate.edu:etd-4305","doi":null,"is_oa":true,"landing_page_url":"https://lib.dr.iastate.edu/etd/13298","pdf_url":"https://lib.dr.iastate.edu/etd/13298","source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://lib.dr.iastate.edu/etd/13298","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068343366","display_name":"Eric E. Parrott","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Eric E. Parrott","raw_affiliation_strings":["Iowa State University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Iowa State University","institution_ids":["https://openalex.org/I173911158"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5068343366"],"corresponding_institution_ids":["https://openalex.org/I173911158"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10091","display_name":"Malaria Research and Control","score":0.36059999465942383,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10091","display_name":"Malaria Research and Control","score":0.36059999465942383,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.1282999962568283,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.10620000213384628,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/plasmodium-falciparum","display_name":"Plasmodium falciparum","score":0.9980000257492065},{"id":"https://openalex.org/keywords/apicoplast","display_name":"apicoplast","score":0.9580000042915344},{"id":"https://openalex.org/keywords/dna-polymerase","display_name":"DNA polymerase","score":0.8040000200271606},{"id":"https://openalex.org/keywords/polymerase-fidelity","display_name":"polymerase fidelity","score":0.7710000276565552},{"id":"https://openalex.org/keywords/pre-steady-state-kinetics","display_name":"pre-steady-state kinetics","score":0.8259999752044678},{"id":"https://openalex.org/keywords/processivity","display_name":"processivity","score":0.6570000052452087},{"id":"https://openalex.org/keywords/mismatch-extension","display_name":"mismatch extension","score":0.6880000233650208},{"id":"https://openalex.org/keywords/3-5-exonuclease","display_name":"3'-5' exonuclease","score":0.8600000143051147},{"id":"https://openalex.org/keywords/apicomplexa","display_name":"Apicomplexa","score":0.6710000038146973},{"id":"https://openalex.org/keywords/antimalarial-drug-targets","display_name":"antimalarial drug targets","score":0.7110000252723694},{"id":"https://openalex.org/keywords/dna-replication","display_name":"DNA replication","score":0.5600000023841858}],"concepts":[{"id":"https://openalex.org/C2775855988","wikidata":"https://www.wikidata.org/wiki/Q1825478","display_name":"Apicoplast","level":5,"score":0.7878886461257935},{"id":"https://openalex.org/C2778371730","wikidata":"https://www.wikidata.org/wiki/Q311383","display_name":"Plasmodium falciparum","level":3,"score":0.6148340106010437},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.5623610019683838},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.48696526885032654},{"id":"https://openalex.org/C2756471","wikidata":"https://www.wikidata.org/wiki/Q206286","display_name":"DNA polymerase","level":3,"score":0.4464419186115265},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4352433979511261},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3701353669166565},{"id":"https://openalex.org/C2781023229","wikidata":"https://www.wikidata.org/wiki/Q193030","display_name":"Apicomplexa","level":4,"score":0.35414814949035645},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.34236282110214233},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.3065771460533142},{"id":"https://openalex.org/C2778048844","wikidata":"https://www.wikidata.org/wiki/Q12156","display_name":"Malaria","level":2,"score":0.29931336641311646},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.12116566300392151}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:lib.dr.iastate.edu:etd-4305","doi":null,"is_oa":true,"landing_page_url":"https://lib.dr.iastate.edu/etd/13298","pdf_url":"https://lib.dr.iastate.edu/etd/13298","source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"},{"id":"mag:72954606","doi":null,"is_oa":false,"landing_page_url":"https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=4305&context=etd","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:lib.dr.iastate.edu:etd-4305","doi":null,"is_oa":true,"landing_page_url":"https://lib.dr.iastate.edu/etd/13298","pdf_url":"https://lib.dr.iastate.edu/etd/13298","source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306274","display_name":"Roy J. Carver Charitable Trust","ror":"https://ror.org/01ah2ch91"},{"id":"https://openalex.org/F4320311090","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W72954606.pdf","grobid_xml":"https://content.openalex.org/works/W72954606.grobid-xml"},"referenced_works_count":34,"referenced_works":["https://openalex.org/W114565725","https://openalex.org/W1491213594","https://openalex.org/W1539813750","https://openalex.org/W1597138031","https://openalex.org/W1973488583","https://openalex.org/W1987045272","https://openalex.org/W1990083018","https://openalex.org/W1990617810","https://openalex.org/W1994567145","https://openalex.org/W1998567654","https://openalex.org/W1999832946","https://openalex.org/W2005105487","https://openalex.org/W2008233255","https://openalex.org/W2018582139","https://openalex.org/W2020592685","https://openalex.org/W2020755315","https://openalex.org/W2023511280","https://openalex.org/W2024166392","https://openalex.org/W2046665996","https://openalex.org/W2049679577","https://openalex.org/W2056997115","https://openalex.org/W2065874951","https://openalex.org/W2094046881","https://openalex.org/W2097812130","https://openalex.org/W2100179055","https://openalex.org/W2103585578","https://openalex.org/W2110343132","https://openalex.org/W2132673947","https://openalex.org/W2138476183","https://openalex.org/W2140983138","https://openalex.org/W2152112318","https://openalex.org/W2161311583","https://openalex.org/W2167268115","https://openalex.org/W2171254101"],"related_works":["https://openalex.org/W2005105487","https://openalex.org/W86942935","https://openalex.org/W4306644059","https://openalex.org/W2100179055","https://openalex.org/W4308550081","https://openalex.org/W7171754597","https://openalex.org/W7096209823","https://openalex.org/W4415263215","https://openalex.org/W1126245994","https://openalex.org/W2483403425"],"abstract_inverted_index":{"Members":[0],"of":[1,30,65,77,94,109,128,142,162,180,195,208,223,231],"the":[2,28,41,61,66,78,83,92,95,117,120,140,149,153,171,181,190,193,209,221,224,227,232,244,252],"phylum":[3],"Apicomplexa,":[4],"including":[5],"pathogens":[6],"such":[7],"as":[8,49,56,160],"Plasmodium":[9],"falciparum,":[10],"Toxoplasma":[11],"gondii,":[12],"and":[13,37,63,123,235,264],"Babesia":[14],"bovis":[15],"contain":[16],"a":[17,50,110,145,163,256],"non-photosynthetic":[18],"plastid":[19,45],"called":[20],"an":[21,125],"apicoplast.":[22,84],"It":[23],"has":[24,74,124],"been":[25],"implicated":[26],"in":[27,70,116],"biosynthesis":[29],"fatty":[31],"acids,":[32],"iron-sulfur":[33],"clusters,":[34],"heme":[35],"groups,":[36],"isoprenoid":[38],"synthesis":[39],"for":[40,53,60,157,258],"pathogen.":[42],"This":[43,201],"unique":[44],"was":[46,166,185,241],"immediately":[47],"recognized":[48],"potential":[51],"target":[52],"drug":[54],"discovery":[55],"it":[57,240],"is":[58,152,198],"essential":[59],"survival":[62],"reproduction":[64],"parasite.":[67],"With":[68],"this":[69],"mind,":[71],"our":[72,88],"group":[73],"initiated":[75],"characterization":[76],"DNA":[79,96],"replication":[80],"system":[81],"within":[82],"Here":[85],"we":[86],"report":[87],"efforts":[89],"to":[90,119,169,251],"determine":[91],"fidelity":[93,112],"polymerase":[97,115],"(apPOL)":[98],"using":[99],"pre\u2013steady":[100],"state":[101],"kinetics.":[102],"The":[103,136],"apPOL":[104,234,245],"exhibits":[105],"error":[106],"rates":[107],"typical":[108],"high":[111],"family":[113],"A":[114],"104":[118],"106":[121],"range":[122],"average":[126],"processivity":[127],"approximately":[129],"2":[130],"nucleotides":[131],"incorporated":[132],"per":[133],"binding":[134],"event.":[135],"data":[137],"indicates":[138],"that":[139,188,205,243],"rate":[141,154],"chemistry":[143],"or":[144],"conformational":[146],"change":[147],"preceding":[148],"chemical":[150],"step":[151,156,165],"limiting":[155],"sequential":[158],"polymerization":[159],"inclusion":[161],"translocation":[164],"not":[167],"necessary":[168],"explain":[170],"product":[172],"time":[173],"courses.":[174],"Additionally,":[175],"with":[176,255],"some":[177],"mismatches,":[178],"stalling":[179,202],"primer":[182,253],"extension":[183,197],"reactions":[184],"observed,":[186],"indicating":[187],"like":[189],"misincorporation":[191,213],"reaction,":[192],"degree":[194],"mismatch":[196,216,225],"highly":[199],"variable.":[200],"may":[203],"indicate":[204],"severe":[206],"distortions":[207],"active":[210],"site":[211],"following":[212],"significantly":[214],"slows":[215],"extension,":[217],"which":[218],"could":[219],"increase":[220,236],"excision":[222],"by":[226,261],"3'\u20135'":[228],"exonuclease":[229],"activity":[230],"wild\u2013type":[233],"overall":[237],"fidelity.":[238],"Finally,":[239],"observed":[242],"will":[246],"catalyze":[247],"non\u2013template":[248],"nucleotide":[249],"additions":[250],"strand":[254],"preference":[257],"dGTP,":[259],"followed":[260],"dATP,":[262],"dCTP,":[263],"dTTP.":[265]},"counts_by_year":[],"updated_date":"2026-08-30T07:30:35.949966","created_date":"2016-06-24T00:00:00"}