{"id":"https://openalex.org/W72635546","doi":null,"title":"Cloning and sequencing of beta toxin gene of Clostridium perfringens type C.","display_name":"Cloning and sequencing of beta toxin gene of Clostridium perfringens type C.","publication_year":2002,"publication_date":"2002-01-01","ids":{"openalex":"https://openalex.org/W72635546","mag":"72635546","pmid":"https://pubmed.ncbi.nlm.nih.gov/12561980"},"language":"en","primary_location":{"id":"pmid:12561980","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12561980","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Indian journal of experimental biology","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112362268","display_name":"P. P. Goswami","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185790649","display_name":"Indian Veterinary Research Institute","ror":"https://ror.org/02jcfzc36","country_code":"IN","type":"facility","lineage":["https://openalex.org/I179420787","https://openalex.org/I185790649"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"P P Goswami","raw_affiliation_strings":["National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India","institution_ids":["https://openalex.org/I185790649"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113765424","display_name":"Kesturu Subbaiah Girish","orcid":"https://orcid.org/0000-0002-7284-3707","observed_orcids":["https://orcid.org/0000-0002-7284-3707"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K S Girish","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111802187","display_name":"Pallab Chaudhuri","orcid":"https://orcid.org/0000-0001-7715-4903","observed_orcids":["https://orcid.org/0000-0001-7715-4903"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P Chaudhuri","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111686515","display_name":"Vinita Tiwari","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"V Tiwari","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084740122","display_name":"S J Akare","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S J Akare","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5043217744","display_name":"P. C. Harbola","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P C Harbola","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.00717107,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"40","issue":"1","first_page":"109","last_page":"10"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11288","display_name":"Clostridium difficile and Clostridium perfringens research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11288","display_name":"Clostridium difficile and Clostridium perfringens research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11274","display_name":"Botulinum Toxin and Related Neurological Disorders","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12499","display_name":"Diphtheria, Corynebacterium, and Tetanus","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/clostridium-perfringens-type-c","display_name":"Clostridium perfringens type C","score":0.949999988079071},{"id":"https://openalex.org/keywords/beta-toxin","display_name":"beta toxin","score":0.8740000128746033},{"id":"https://openalex.org/keywords/toxin-gene-cloning","display_name":"toxin gene cloning","score":0.7549999952316284},{"id":"https://openalex.org/keywords/gene-sequencing","display_name":"gene sequencing","score":0.7129999995231628},{"id":"https://openalex.org/keywords/gene-amplification","display_name":"gene amplification","score":0.5839999914169312}],"concepts":[{"id":"https://openalex.org/C2779116991","wikidata":"https://www.wikidata.org/wiki/Q309465","display_name":"Clostridium perfringens","level":3,"score":0.8574013710021973},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.7091021537780762},{"id":"https://openalex.org/C2777367657","wikidata":"https://www.wikidata.org/wiki/Q184651","display_name":"Toxin","level":2,"score":0.6722060441970825},{"id":"https://openalex.org/C84148353","wikidata":"https://www.wikidata.org/wiki/Q863908","display_name":"Nucleic acid sequence","level":3,"score":0.6333342790603638},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.619935154914856},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.596895158290863},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.5781116485595703},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.5054478645324707},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.49713972210884094},{"id":"https://openalex.org/C2776174256","wikidata":"https://www.wikidata.org/wiki/Q830842","display_name":"BETA (programming language)","level":2,"score":0.4807465076446533},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4230687916278839},{"id":"https://openalex.org/C2781092032","wikidata":"https://www.wikidata.org/wiki/Q327663","display_name":"Clostridium","level":3,"score":0.4140760600566864},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.32979631423950195},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.3186354637145996},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.11356467008590698},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001427","descriptor_name":"Bacterial Toxins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003016","descriptor_name":"Clostridium perfringens","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D003016","descriptor_name":"Clostridium perfringens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015139","descriptor_name":"Blotting, Southern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015183","descriptor_name":"Restriction Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:12561980","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12561980","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Indian journal of experimental biology","raw_type":"Journal Article"},{"id":"mag:72635546","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12561980","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2361608796","https://openalex.org/W2899236103","https://openalex.org/W2360366374","https://openalex.org/W2381355018","https://openalex.org/W2367921317","https://openalex.org/W2357766396","https://openalex.org/W2365733669","https://openalex.org/W1951259843","https://openalex.org/W2352780019","https://openalex.org/W2384112064"],"abstract_inverted_index":{"A":[0],"gene":[1,35,44],"encoding":[2],"beta":[3,33,42,54],"toxin":[4,34,43],"was":[5,26],"amplified":[6],"by":[7],"polymerase":[8],"chain":[9],"reaction":[10],"from":[11],"C.":[12,29,56],"perfringens":[13,30],"type":[14,31],"C":[15],"isolate":[16],"and":[17],"cloned":[18],"in":[19,53],"pUC":[20],"19":[21],"vector.":[22],"The":[23,37],"nucleotide":[24],"sequence":[25],"identical":[27],"with":[28],"B":[32],"sequence.":[36],"Southern":[38],"hybridization":[39],"using":[40],"labelled":[41],"probe":[45],"revealed":[46],"the":[47],"presence":[48],"of":[49],"positive":[50],"signals":[51],"only":[52],"producing":[55],"perfingens.":[57]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}