{"id":"https://openalex.org/W7225901929","doi":"https://doi.org/10.1021/acsami.6c22110","title":"GSH/pH Dual-Responsive Zwitterionic mRNA Nanocomplexes for Tumor-Enriched mRNA Delivery in a B16F10 Melanoma Model","display_name":"GSH/pH Dual-Responsive Zwitterionic mRNA Nanocomplexes for Tumor-Enriched mRNA Delivery in a B16F10 Melanoma Model","publication_year":2026,"publication_date":"2026-11-13","ids":{"openalex":"https://openalex.org/W7225901929","doi":"https://doi.org/10.1021/acsami.6c22110","pmid":"https://pubmed.ncbi.nlm.nih.gov/43020114"},"language":"en","primary_location":{"id":"doi:10.1021/acsami.6c22110","doi":"https://doi.org/10.1021/acsami.6c22110","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acsami.6c22110","pdf_url":null,"source":{"id":"https://openalex.org/S164001016","display_name":"ACS Applied Materials & Interfaces","issn_l":"1944-8244","issn":["1944-8244","1944-8252"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Applied Materials & Interfaces","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W7225901929"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081158241","display_name":"Xueying Zhou","orcid":"https://orcid.org/0009-0006-7398-3863","observed_orcids":["https://orcid.org/0009-0006-7398-3863"]},"institutions":[{"id":"https://openalex.org/I4210100868","display_name":"Second Affiliated Hospital of Dalian Medical University","ror":"https://ror.org/012f2cn18","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210100868"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xueying Zhou","raw_affiliation_strings":["The Second Affiliated Hospital of Dalian Medical University , , No. 467 Zhongshan Road, Shahekou District , ,"],"raw_orcid":"https://orcid.org/0009-0006-7398-3863","affiliations":[{"raw_affiliation_string":"The Second Affiliated Hospital of Dalian Medical University , , No. 467 Zhongshan Road, Shahekou District , ,","institution_ids":["https://openalex.org/I4210100868"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101939602","display_name":"Sha Wu","orcid":"https://orcid.org/0000-0003-2802-7806","observed_orcids":["https://orcid.org/0000-0003-2802-7806","https://orcid.org/0000-0002-4185-3275"]},"institutions":[{"id":"https://openalex.org/I4210100868","display_name":"Second Affiliated Hospital of Dalian Medical University","ror":"https://ror.org/012f2cn18","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210100868"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Sha Wu","raw_affiliation_strings":["The Second Affiliated Hospital of Dalian Medical University , , No. 467 Zhongshan Road, Shahekou District , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Second Affiliated Hospital of Dalian Medical University , , No. 467 Zhongshan Road, Shahekou District , ,","institution_ids":["https://openalex.org/I4210100868"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161630574","display_name":"Lijun Chang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lijun Chang","raw_affiliation_strings":["Dalian Medical University , , No. 9 West Section of Lvshun South Road, Lvshunkou District , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Medical University , , No. 9 West Section of Lvshun South Road, Lvshunkou District , ,","institution_ids":["https://openalex.org/I191996457"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161697280","display_name":"Ying Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I191996457","display_name":"Dalian Medical University","ror":"https://ror.org/04c8eg608","country_code":"CN","type":"education","lineage":["https://openalex.org/I191996457"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ying Wang","raw_affiliation_strings":["Dalian Medical University , , No. 9 West Section of Lvshun South Road, Lvshunkou District , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dalian Medical University , , No. 9 West Section of Lvshun South Road, Lvshunkou District , ,","institution_ids":["https://openalex.org/I191996457"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100948494","display_name":"Tangna Hao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210100868","display_name":"Second Affiliated Hospital of Dalian Medical University","ror":"https://ror.org/012f2cn18","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210100868"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Tangna Hao","raw_affiliation_strings":["The Second Affiliated Hospital of Dalian Medical University , , No. 467 Zhongshan Road, Shahekou District , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Second Affiliated Hospital of Dalian Medical University , , No. 467 Zhongshan Road, Shahekou District , ,","institution_ids":["https://openalex.org/I4210100868"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5049713067","display_name":"Jia Yu","orcid":"https://orcid.org/0000-0001-8342-5423","observed_orcids":["https://orcid.org/0000-0001-8342-5423","https://orcid.org/0009-0005-8535-5576"]},"institutions":[{"id":"https://openalex.org/I4210100868","display_name":"Second Affiliated Hospital of Dalian Medical University","ror":"https://ror.org/012f2cn18","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210100868"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Jia Yu","raw_affiliation_strings":["The Second Affiliated Hospital of Dalian Medical University , , No. 467 Zhongshan Road, Shahekou District , ,"],"raw_orcid":"https://orcid.org/0009-0005-8535-5576","affiliations":[{"raw_affiliation_string":"The Second Affiliated Hospital of Dalian Medical University , , No. 467 Zhongshan Road, Shahekou District , ,","institution_ids":["https://openalex.org/I4210100868"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5049713067"],"corresponding_institution_ids":["https://openalex.org/I4210100868"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.9735999703407288,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.9735999703407288,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10063","display_name":"Nanoparticle-Based Drug Delivery","score":0.025100000202655792,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11686","display_name":"Dendrimers and Hyperbranched Polymers","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2507","display_name":"Polymers and Plastics"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ph-responsive-drug-release","display_name":"pH-responsive drug release","score":0.8349999785423279},{"id":"https://openalex.org/keywords/2-methacryloyloxyethyl-phosphorylcholine","display_name":"2-methacryloyloxyethyl phosphorylcholine","score":0.8460000157356262},{"id":"https://openalex.org/keywords/1-vinylimidazole","display_name":"1-vinylimidazole","score":0.9470000267028809},{"id":"https://openalex.org/keywords/in-situ-polymerization","display_name":"in situ polymerization","score":0.7390000224113464},{"id":"https://openalex.org/keywords/lysosome-escape","display_name":"lysosomal escape","score":0.703000009059906},{"id":"https://openalex.org/keywords/circulating-half-life","display_name":"serum half-life","score":0.5419999957084656},{"id":"https://openalex.org/keywords/protein-adsorption","display_name":"protein adsorption","score":0.5299999713897705}],"concepts":[{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.7128999829292297},{"id":"https://openalex.org/C111335760","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferase","level":4,"score":0.5631999969482422},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5430999994277954},{"id":"https://openalex.org/C2777807558","wikidata":"https://www.wikidata.org/wiki/Q4914764","display_name":"Biodistribution","level":3,"score":0.5235999822616577},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.520799994468689},{"id":"https://openalex.org/C11432220","wikidata":"https://www.wikidata.org/wiki/Q1975334","display_name":"Dispersity","level":2,"score":0.5117999911308289},{"id":"https://openalex.org/C538909803","wikidata":"https://www.wikidata.org/wiki/Q116907","display_name":"Glutathione","level":3,"score":0.4970000088214874},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4936000108718872},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.4339999854564667},{"id":"https://openalex.org/C2777271071","wikidata":"https://www.wikidata.org/wiki/Q422529","display_name":"Nuclease","level":3,"score":0.42320001125335693},{"id":"https://openalex.org/C2780801859","wikidata":"https://www.wikidata.org/wiki/Q2013175","display_name":"Glycidyl methacrylate","level":4,"score":0.4122999906539917},{"id":"https://openalex.org/C2776833585","wikidata":"https://www.wikidata.org/wiki/Q7187582","display_name":"Phosphorylcholine","level":2,"score":0.3919000029563904},{"id":"https://openalex.org/C102747710","wikidata":"https://www.wikidata.org/wiki/Q824237","display_name":"Endosome","level":3,"score":0.3668999969959259},{"id":"https://openalex.org/C2779902561","wikidata":"https://www.wikidata.org/wiki/Q471706","display_name":"Hemolysis","level":2,"score":0.3560999929904938},{"id":"https://openalex.org/C88001094","wikidata":"https://www.wikidata.org/wiki/Q7251453","display_name":"Protein adsorption","level":3,"score":0.3555000126361847},{"id":"https://openalex.org/C44228677","wikidata":"https://www.wikidata.org/wiki/Q181898","display_name":"Polymerization","level":3,"score":0.35249999165534973},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.3472999930381775},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.33219999074935913},{"id":"https://openalex.org/C18150654","wikidata":"https://www.wikidata.org/wiki/Q17153796","display_name":"Nanocarriers","level":3,"score":0.32600000500679016},{"id":"https://openalex.org/C104628117","wikidata":"https://www.wikidata.org/wiki/Q3964828","display_name":"Sonication","level":2,"score":0.3257000148296356},{"id":"https://openalex.org/C132050475","wikidata":"https://www.wikidata.org/wiki/Q736232","display_name":"Methacrylate","level":4,"score":0.3160000145435333},{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.31279999017715454},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.3095000088214874},{"id":"https://openalex.org/C109316439","wikidata":"https://www.wikidata.org/wiki/Q246181","display_name":"Cytotoxicity","level":3,"score":0.30469998717308044},{"id":"https://openalex.org/C155672457","wikidata":"https://www.wikidata.org/wiki/Q61231","display_name":"Nanoparticle","level":2,"score":0.29899999499320984},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.2987000048160553},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.2913999855518341},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.29100000858306885},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.289900004863739},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.27649998664855957},{"id":"https://openalex.org/C2777658100","wikidata":"https://www.wikidata.org/wiki/Q180614","display_name":"Melanoma","level":2,"score":0.27160000801086426},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.25999999046325684},{"id":"https://openalex.org/C13965031","wikidata":"https://www.wikidata.org/wiki/Q243545","display_name":"Nuclear chemistry","level":1,"score":0.2574000060558319},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.2572999894618988},{"id":"https://openalex.org/C142575336","wikidata":"https://www.wikidata.org/wiki/Q412148","display_name":"Polyadenylation","level":4,"score":0.2556000053882599},{"id":"https://openalex.org/C7623868","wikidata":"https://www.wikidata.org/wiki/Q1967135","display_name":"Centrifugation","level":2,"score":0.25440001487731934}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1021/acsami.6c22110","doi":"https://doi.org/10.1021/acsami.6c22110","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acsami.6c22110","pdf_url":null,"source":{"id":"https://openalex.org/S164001016","display_name":"ACS Applied Materials & Interfaces","issn_l":"1944-8244","issn":["1944-8244","1944-8252"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Applied Materials & Interfaces","raw_type":"journal-article"},{"id":"pmid:43020114","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/43020114","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS applied materials & interfaces","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9391000270843506}],"study_designs":[],"awards":[{"id":"https://openalex.org/G9001350870","display_name":null,"funder_award_id":"2025-MSLH-177","funder_id":"https://openalex.org/F4320323086","funder_display_name":"Natural Science Foundation of Liaoning Province"}],"funders":[{"id":"https://openalex.org/F4320323086","display_name":"Natural Science Foundation of Liaoning Province","ror":null}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":32,"referenced_works":["https://openalex.org/W1963706689","https://openalex.org/W1966090623","https://openalex.org/W1973831032","https://openalex.org/W1995997091","https://openalex.org/W2008028087","https://openalex.org/W2009841111","https://openalex.org/W2010828967","https://openalex.org/W2029749273","https://openalex.org/W2040071171","https://openalex.org/W2050886209","https://openalex.org/W2074880818","https://openalex.org/W2079445254","https://openalex.org/W2170405846","https://openalex.org/W2573033399","https://openalex.org/W2605233023","https://openalex.org/W2782708334","https://openalex.org/W3004416358","https://openalex.org/W3109746568","https://openalex.org/W3122312485","https://openalex.org/W3130729977","https://openalex.org/W3148255637","https://openalex.org/W3164967630","https://openalex.org/W3167513896","https://openalex.org/W3184644021","https://openalex.org/W3188940990","https://openalex.org/W4205824800","https://openalex.org/W4210992847","https://openalex.org/W4229452462","https://openalex.org/W4292328694","https://openalex.org/W4402191607","https://openalex.org/W4415219316","https://openalex.org/W7171446677"],"related_works":[],"abstract_inverted_index":{"Messenger":[0],"RNA":[1,304],"(mRNA)":[2],"therapy":[3],"offers":[4],"a":[5,27,72,163,195,209,225,265,281,323,353],"programmable":[6],"route":[7],"to":[8,100,124],"melanoma":[9],"treatment,":[10],"but":[11],"its":[12],"translation":[13],"is":[14],"limited":[15,96],"by":[16,35,136],"nuclease":[17],"degradation,":[18],"off-target":[19],"biodistribution":[20],"and":[21,47,71,116,197,204,252,295,332,347],"endolysosomal":[22],"trapping.":[23],"Here":[24],"we":[25],"report":[26],"glutathione":[28],"(GSH)/pH":[29],"dual-responsive":[30],"mRNA":[31,53,123,300,338],"nanocomplex":[32,190,326],"(mRNA-NC)":[33],"fabricated":[34],"one-pot":[36],"in":[37,162,194,208],"situ":[38],"polymerization":[39],"of":[40,59,67,75,84,121,142,231,285],"N-(3-aminopropyl)methacrylamide":[41],"(APMA),":[42],"1-vinylimidazole":[43],"(VI),":[44],"N,N'-bis(acryloyl)cystamine":[45],"(BACA),":[46],"2-methacryloyloxyethyl":[48],"phosphorylcholine":[49],"(MPC)":[50],"on":[51,344],"the":[52,61,118,122,143,189,201],"template.":[54],"At":[55],"an":[56,64],"N/P":[57],"ratio":[58],"40,":[60],"carrier":[62],"reached":[63],"encapsulation":[65],"efficiency":[66],"92.7":[68],"\u00b1":[69,77,86,102,114,140,175,179,233,248,254,287,291],"2.1%":[70],"loading":[73],"capacity":[74],"87.4":[76],"3.2":[78],"\u03bcg":[79,104],"mg-1,":[80],"forming":[81],"monodisperse":[82],"particles":[83],"126.4":[85],"7.2":[87],"nm":[88],"(polydispersity":[89],"index,":[90],"PDI,":[91],"0.14).":[92],"The":[93],"zwitterionic":[94,325],"corona":[95],"plasma":[97],"protein":[98,237],"adsorption":[99],"12.6":[101],"1.5":[103],"mg-1":[105,236],"(4.6-fold":[106],"lower":[107],"than":[108,130,244],"jetPEI/mRNA),":[109],"kept":[110],"hemolysis":[111],"at":[112,181,238,278,305,314],"0.8":[113],"0.2%,":[115],"extended":[117],"serum":[119],"half-life":[120],"\u224827.4":[125],"h,":[126,240,307],"roughly":[127],"9-fold":[128],"longer":[129],"naked":[131],"mRNA.":[132,362],"Release":[133],"was":[134,145,191,220],"gated":[135],"both":[137],"triggers:":[138],"86.5":[139],"3.7%":[141],"cargo":[144],"released":[146,173],"within":[147],"8":[148],"h":[149,165],"under":[150,156],"10":[151,170],"mM":[152,171],"GSH,":[153],"whereas":[154],"leakage":[155],"physiological":[157],"conditions":[158],"remained":[159,313],"\u2264":[160],"12%;":[161],"4":[164],"matrix,":[166],"pH":[167,182,219],"5.5":[168],"plus":[169],"GSH":[172],"85":[174],"6.8%":[176],"versus":[177],"12":[178,306],"1.5%":[180],"7.4":[183],"without":[184],"GSH.":[185],"In":[186,260],"B16F10":[187],"cells,":[188],"taken":[192],"up":[193],"dose-":[196],"energy-dependent":[198],"manner,":[199],"entered":[200],"lysosomal":[202],"compartment,":[203],"escaped":[205],"over":[206],"time":[207],"manner":[210],"consistent":[211],"with":[212,246,360],"imidazole-mediated":[213],"proton-sponge":[214],"buffering":[215],"(a":[216],"hypothesis;":[217],"intralysosomal":[218],"not":[221],"measured":[222],"directly),":[223],"producing":[224],"peak":[226],"firefly":[227],"luciferase":[228],"(FLuc)":[229],"expression":[230],"56,140":[232],"3280":[234],"RLU":[235],"24":[239],"about":[241],"1.5-fold":[242],"higher":[243],"jetPEI/mRNA,":[245],"93.6":[247],"3.9%":[249],"cell":[250],"viability":[251],"6.7":[253],"1.2%":[255],"lactate":[256],"dehydrogenase":[257],"(LDH)":[258],"release.":[259],"B16F10-bearing":[261],"albino":[262],"C57BL/6":[263],"mice,":[264],"single":[266,324],"intravenous":[267],"dose":[268],"produced":[269],"tumor-dominant":[270],"bioluminescence":[271],"(peak":[272],"5.7":[273],"\u00d7":[274,297],"106":[275],"photons":[276],"s-1":[277],"6":[279],"h),":[280],"tumor":[282,303],"enrichment":[283],"index":[284],"1.22":[286],"0.18":[288],"(vs":[289],"0.31":[290],"0.03":[292],"for":[293,335,356],"jetPEI/mRNA)":[294],"2.42":[296],"107":[298],"FLuc":[299],"copies":[301],"\u03bcg-1":[302],"while":[308],"all":[309],"7-day":[310],"safety":[311],"indicators":[312],"phosphate-buffered":[315],"saline":[316],"(PBS)":[317],"levels.":[318],"These":[319],"results":[320],"demonstrate":[321],"that":[322],"can":[327],"integrate":[328],"stealth,":[329],"dual-triggered":[330],"release,":[331],"endosomal":[333],"escape":[334],"safe,":[336],"tumor-enriched":[337],"delivery.":[339],"While":[340],"this":[341],"study":[342],"focuses":[343],"delivery":[345],"performance":[346],"reporter":[348],"gene":[349],"expression,":[350],"it":[351],"lays":[352],"solid":[354],"foundation":[355],"future":[357],"therapeutic":[358],"applications":[359],"functional":[361]},"counts_by_year":[],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2026-11-13T00:00:00"}