{"id":"https://openalex.org/W7225830757","doi":"https://doi.org/10.64898/2026.11.09.764842","title":"Effect of Lactobacillus postbiotic supplementation on intestinal permeability, nutrient digestibility, and gut microbial and metabolic profiles in aging cats","display_name":"Effect of Lactobacillus postbiotic supplementation on intestinal permeability, nutrient digestibility, and gut microbial and metabolic profiles in aging cats","publication_year":2026,"publication_date":"2026-11-10","ids":{"openalex":"https://openalex.org/W7225830757","doi":"https://doi.org/10.64898/2026.11.09.764842"},"language":"en","primary_location":{"id":"doi:10.64898/2026.11.09.764842","doi":"https://doi.org/10.64898/2026.11.09.764842","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.09.764842","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.11.09.764842","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040953293","display_name":"Sergio Andreu\u2010S\u00e1nchez","orcid":"https://orcid.org/0000-0002-3503-9971","observed_orcids":["https://orcid.org/0000-0002-3503-9971"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sergio Andreu-Sanchez","raw_affiliation_strings":["dsm-firmenich, Biotech Campus, Delft, The Netherlands;"],"raw_orcid":"https://orcid.org/0000-0002-3503-9971","affiliations":[{"raw_affiliation_string":"dsm-firmenich, Biotech Campus, Delft, The Netherlands;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021831453","display_name":"B\u00e1lint Tam\u00e1si","orcid":"https://orcid.org/0000-0002-2629-7362","observed_orcids":["https://orcid.org/0000-0002-2629-7362"]},"institutions":[{"id":"https://openalex.org/I4405267139","display_name":"DSM-Firmenich AG (Switzerland)","ror":"https://ror.org/01c00kj47","country_code":"CH","type":"company","lineage":["https://openalex.org/I4405267139"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Balint Tamasi","raw_affiliation_strings":["dsm-firmenich AG, Kaiseraugst, Switzerland;"],"raw_orcid":"https://orcid.org/0000-0002-2629-7362","affiliations":[{"raw_affiliation_string":"dsm-firmenich AG, Kaiseraugst, Switzerland;","institution_ids":["https://openalex.org/I4405267139"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009671839","display_name":"Anne-Sophie Boucard","orcid":"https://orcid.org/0000-0002-8109-0954","observed_orcids":["https://orcid.org/0000-0002-8109-0954"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Anne-Sophie Boucard","raw_affiliation_strings":["dsm-firmenich Houdan SAS, Houdan, France;"],"raw_orcid":"https://orcid.org/0000-0002-8109-0954","affiliations":[{"raw_affiliation_string":"dsm-firmenich Houdan SAS, Houdan, France;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022625741","display_name":"Elena Pagani","orcid":"https://orcid.org/0000-0002-3359-8921","observed_orcids":["https://orcid.org/0000-0002-3359-8921"]},"institutions":[{"id":"https://openalex.org/I4405267139","display_name":"DSM-Firmenich AG (Switzerland)","ror":"https://ror.org/01c00kj47","country_code":"CH","type":"company","lineage":["https://openalex.org/I4405267139"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Elena Pagani","raw_affiliation_strings":["dsm-firmenich AG, Kaiseraugst, Switzerland;"],"raw_orcid":"https://orcid.org/0000-0002-3359-8921","affiliations":[{"raw_affiliation_string":"dsm-firmenich AG, Kaiseraugst, Switzerland;","institution_ids":["https://openalex.org/I4405267139"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091438247","display_name":"Marco A. van den Berg","orcid":"https://orcid.org/0000-0003-2770-9944","observed_orcids":["https://orcid.org/0000-0003-2770-9944"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Marco A. van den Berg","raw_affiliation_strings":["dsm-firmenich, Biotech Campus, Delft, The Netherlands;"],"raw_orcid":"https://orcid.org/0000-0003-2770-9944","affiliations":[{"raw_affiliation_string":"dsm-firmenich, Biotech Campus, Delft, The Netherlands;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001415769","display_name":"Brian A. Klein","orcid":"https://orcid.org/0000-0002-7233-2114","observed_orcids":["https://orcid.org/0000-0002-7233-2114"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Brian Klein","raw_affiliation_strings":["dsm-firmenich Biotechnology Dept., Lexington, MA, USA;"],"raw_orcid":"https://orcid.org/0000-0002-7233-2114","affiliations":[{"raw_affiliation_string":"dsm-firmenich Biotechnology Dept., Lexington, MA, USA;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053663841","display_name":"Daniel R. Bernstein","orcid":"https://orcid.org/0000-0003-4262-3348","observed_orcids":["https://orcid.org/0000-0003-4262-3348"]},"institutions":[{"id":"https://openalex.org/I41125697","display_name":"AgResearch","ror":"https://ror.org/0124gwh94","country_code":"NZ","type":"government","lineage":["https://openalex.org/I41125697","https://openalex.org/I4405260297"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Daniel R. Bernstein","raw_affiliation_strings":["Smart Foods & Bioproducts Group, AgResearch, New Zealand Institute for Bioeconomy Science Limited, Palmerston North, New Zealand;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Smart Foods & Bioproducts Group, AgResearch, New Zealand Institute for Bioeconomy Science Limited, Palmerston North, New Zealand;","institution_ids":["https://openalex.org/I41125697"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049693734","display_name":"Margreet Hekman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I51158804","display_name":"Massey University","ror":"https://ror.org/052czxv31","country_code":"NZ","type":"education","lineage":["https://openalex.org/I51158804"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Margreet Hekman","raw_affiliation_strings":["School of Agriculture and Environment, Massey University, Palmerston North, New Zealand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Agriculture and Environment, Massey University, Palmerston North, New Zealand","institution_ids":["https://openalex.org/I51158804"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079461207","display_name":"David G. Thomas","orcid":"https://orcid.org/0000-0001-7460-9351","observed_orcids":["https://orcid.org/0000-0001-7460-9351"]},"institutions":[{"id":"https://openalex.org/I51158804","display_name":"Massey University","ror":"https://ror.org/052czxv31","country_code":"NZ","type":"education","lineage":["https://openalex.org/I51158804"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"David G. Thomas","raw_affiliation_strings":["School of Agriculture and Environment, Massey University, Palmerston North, New Zealand"],"raw_orcid":"https://orcid.org/0000-0001-7460-9351","affiliations":[{"raw_affiliation_string":"School of Agriculture and Environment, Massey University, Palmerston North, New Zealand","institution_ids":["https://openalex.org/I51158804"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081578292","display_name":"Karl Fraser","orcid":"https://orcid.org/0000-0002-1136-4024","observed_orcids":["https://orcid.org/0000-0002-1136-4024"]},"institutions":[{"id":"https://openalex.org/I41125697","display_name":"AgResearch","ror":"https://ror.org/0124gwh94","country_code":"NZ","type":"government","lineage":["https://openalex.org/I41125697","https://openalex.org/I4405260297"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Karl Fraser","raw_affiliation_strings":["Smart Foods & Bioproducts Group, AgResearch, New Zealand Institute for Bioeconomy Science Limited, Palmerston North, New Zealand;"],"raw_orcid":"https://orcid.org/0000-0002-1136-4024","affiliations":[{"raw_affiliation_string":"Smart Foods & Bioproducts Group, AgResearch, New Zealand Institute for Bioeconomy Science Limited, Palmerston North, New Zealand;","institution_ids":["https://openalex.org/I41125697"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091289187","display_name":"Erik R. M. Eckhardt","orcid":"https://orcid.org/0000-0002-3015-5180","observed_orcids":["https://orcid.org/0000-0002-3015-5180"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Erik Eckhardt","raw_affiliation_strings":["dsm-firmenich Houdan SAS, Houdan, France;"],"raw_orcid":"https://orcid.org/0000-0002-3015-5180","affiliations":[{"raw_affiliation_string":"dsm-firmenich Houdan SAS, Houdan, France;","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5091289187"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10152","display_name":"Animal Nutrition and Physiology","score":0.5253999829292297,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10152","display_name":"Animal Nutrition and Physiology","score":0.5253999829292297,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10066","display_name":"Gut microbiota and health","score":0.24050000309944153,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.2102999985218048,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C135870905","wikidata":"https://www.wikidata.org/wiki/Q903667","display_name":"Metabolome","level":3,"score":0.8379999995231628},{"id":"https://openalex.org/C2779904517","wikidata":"https://www.wikidata.org/wiki/Q5008900","display_name":"CATS","level":2,"score":0.730400025844574},{"id":"https://openalex.org/C61716771","wikidata":"https://www.wikidata.org/wiki/Q496","display_name":"Feces","level":2,"score":0.7272999882698059},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6521999835968018},{"id":"https://openalex.org/C2781071285","wikidata":"https://www.wikidata.org/wiki/Q17141320","display_name":"Intestinal permeability","level":2,"score":0.6218000054359436},{"id":"https://openalex.org/C142796444","wikidata":"https://www.wikidata.org/wiki/Q181394","display_name":"Nutrient","level":2,"score":0.583899974822998},{"id":"https://openalex.org/C143121216","wikidata":"https://www.wikidata.org/wiki/Q1330402","display_name":"Microbiome","level":2,"score":0.5648000240325928},{"id":"https://openalex.org/C2780206646","wikidata":"https://www.wikidata.org/wiki/Q1061596","display_name":"Lactobacillus","level":3,"score":0.4683000147342682},{"id":"https://openalex.org/C2777226302","wikidata":"https://www.wikidata.org/wiki/Q233345","display_name":"Ileum","level":2,"score":0.4174000024795532},{"id":"https://openalex.org/C42407357","wikidata":"https://www.wikidata.org/wiki/Q521","display_name":"Physiology","level":1,"score":0.4156000018119812},{"id":"https://openalex.org/C539455810","wikidata":"https://www.wikidata.org/wiki/Q739734","display_name":"Gut flora","level":2,"score":0.40790000557899475},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.38280001282691956},{"id":"https://openalex.org/C2908713948","wikidata":"https://www.wikidata.org/wiki/Q104117645","display_name":"Intestinal Microbiome","level":3,"score":0.3707999885082245},{"id":"https://openalex.org/C87813604","wikidata":"https://www.wikidata.org/wiki/Q1242899","display_name":"Crossover study","level":4,"score":0.3691999912261963},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.36149999499320984},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.3431999981403351},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3174000084400177},{"id":"https://openalex.org/C140793950","wikidata":"https://www.wikidata.org/wiki/Q168091","display_name":"Animal science","level":1,"score":0.314300000667572},{"id":"https://openalex.org/C2780255968","wikidata":"https://www.wikidata.org/wiki/Q1816730","display_name":"Probiotic","level":3,"score":0.30079999566078186},{"id":"https://openalex.org/C2777507495","wikidata":"https://www.wikidata.org/wiki/Q185317","display_name":"Cecum","level":2,"score":0.2978000044822693},{"id":"https://openalex.org/C2779932011","wikidata":"https://www.wikidata.org/wiki/Q581885","display_name":"Ussing chamber","level":3,"score":0.2955999970436096},{"id":"https://openalex.org/C21565614","wikidata":"https://www.wikidata.org/wiki/Q12149006","display_name":"Metabolomics","level":2,"score":0.28049999475479126},{"id":"https://openalex.org/C193230392","wikidata":"https://www.wikidata.org/wiki/Q1663054","display_name":"Ingestion","level":2,"score":0.2732999920845032},{"id":"https://openalex.org/C2780305828","wikidata":"https://www.wikidata.org/wiki/Q132656","display_name":"Bifidobacterium","level":4,"score":0.272599995136261},{"id":"https://openalex.org/C2777165150","wikidata":"https://www.wikidata.org/wiki/Q269334","display_name":"Dysbiosis","level":3,"score":0.26409998536109924},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.2639000117778778},{"id":"https://openalex.org/C27081682","wikidata":"https://www.wikidata.org/wiki/Q269829","display_name":"Placebo","level":3,"score":0.25209999084472656}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.11.09.764842","doi":"https://doi.org/10.64898/2026.11.09.764842","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.09.764842","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.11.09.764842","doi":"https://doi.org/10.64898/2026.11.09.764842","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.09.764842","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Aging":[0],"in":[1,18,52,62,145,151,160,166,261],"cats":[2,20,56,79,167,264],"is":[3],"associated":[4,117,139,205],"with":[5,28,118,128,140,191],"declining":[6],"nutrient":[7,90,121,239],"digestibility":[8,122],"and":[9,46,48,94,148,179,214,237,247,250,272],"increased":[10],"intestinal":[11,37,104,113,231,278],"permeability,":[12,38],"but":[13,233],"evidence":[14,132],"supporting":[15],"microbiome-targeted":[16],"interventions":[17],"older":[19,53,263],"remains":[21],"limited.":[22],"We":[23],"investigated":[24],"whether":[25],"daily":[26],"supplementation":[27],"the":[29,86,129,152,257],"Lactobacillus-derived":[30],"postbiotic":[31],"LB":[32],"fermentate":[33],"HF":[34],"(LBHF)":[35],"affected":[36],"apparent":[39],"total":[40],"tract":[41],"digestibility,":[42,91,240,243],"gut":[43],"microbiome":[44,92,213,249],"composition,":[45,93],"fecal":[47,181,206],"plasma":[49,178],"metabolomic":[50,95],"profiles":[51,96],"cats.":[54],"Twenty":[55],"aged":[57],"7.7-13.8":[58],"years":[59],"were":[60,97],"enrolled":[61],"a":[63,75,141,149,174],"blinded,":[64],"placebo-controlled":[65],"crossover":[66],"trial":[67],"comprising":[68],"two":[69],"29-day":[70],"treatment":[71,189],"periods":[72],"separated":[73],"by":[74,171],"28-day":[76],"washout;":[77],"18":[78],"completed":[80],"both":[81],"periods.":[82],"Intestinal":[83],"permeability":[84,105,114,232],"was":[85,115,123,138,163,234],"primary":[87],"endpoint,":[88],"while":[89,244],"secondary":[98],"endpoints.":[99],"LBHF":[100,126,137,188,201,227,260],"did":[101,228],"not":[102,229],"affect":[103],"or":[106,223],"absorptive":[107],"capacity":[108],"relative":[109,153],"to":[110,269,277],"placebo,":[111],"although":[112],"positively":[116],"age.":[119],"Overall":[120],"higher":[124],"during":[125,187,200],"treatment,":[127,202],"clearest":[130],"endpoint-specific":[131],"observed":[133],"for":[134],"protein":[135,242],"digestibility.":[136],"non-significant":[142],"overall":[143],"increase":[144,159],"Shannon":[146,161],"diversity":[147,162],"reduction":[150],"abundance":[154],"of":[155,259],"Streptococcus":[156],"lutetiensis.":[157],"The":[158],"significant":[164],"only":[165],"receiving":[168],"placebo":[169],"followed":[170],"LBHF,":[172],"indicating":[173],"treatment-sequence-dependent":[175],"response.":[176],"Thirty-three":[177],"29":[180],"metabolites":[182,194,207],"showed":[183,196,208],"significantly":[184],"different":[185],"changes":[186,199],"compared":[190],"placebo.":[192],"Plasma":[193],"generally":[195],"more":[197,209,267],"positive":[198],"whereas":[203],"most":[204],"negative":[210],"changes.":[211],"Sequence-stratified":[212],"metabolome":[215,251],"responses":[216,275],"differed":[217],"substantially,":[218],"suggesting":[219],"possible":[220],"period,":[221],"treatment-order,":[222],"carryover":[224],"effects.":[225],"Overall,":[226],"improve":[230],"well":[235],"tolerated":[236],"improved":[238],"particularly":[241],"producing":[245],"modest":[246],"sequence-dependent":[248],"responses.":[252],"These":[253],"findings":[254],"suggest":[255],"that":[256],"effects":[258],"healthy":[262],"may":[265],"relate":[266],"strongly":[268],"digestive":[270],"efficiency":[271],"host":[273],"metabolic":[274],"than":[276],"permeability.":[279]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2026-11-12T00:00:00"}