{"id":"https://openalex.org/W7225827116","doi":"https://doi.org/10.1021/acs.jafc.6c13632","title":"Advances in Cystathionine \u03b2-Lyase-Catalyzed Synthesis of Thiosulfinates: Enzyme Resource Mining, Catalytic Mechanism, and Structure-Guided Molecular Engineering","display_name":"Advances in Cystathionine \u03b2-Lyase-Catalyzed Synthesis of Thiosulfinates: Enzyme Resource Mining, Catalytic Mechanism, and Structure-Guided Molecular Engineering","publication_year":2026,"publication_date":"2026-11-11","ids":{"openalex":"https://openalex.org/W7225827116","doi":"https://doi.org/10.1021/acs.jafc.6c13632"},"language":"en","primary_location":{"id":"doi:10.1021/acs.jafc.6c13632","doi":"https://doi.org/10.1021/acs.jafc.6c13632","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.jafc.6c13632","pdf_url":null,"source":{"id":"https://openalex.org/S134644764","display_name":"Journal of Agricultural and Food Chemistry","issn_l":"0021-8561","issn":["0021-8561","1520-5118"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Agricultural and Food Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5039613669","display_name":"Yuanxiang Liu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405279728","display_name":"Qilu Medical University","ror":"https://ror.org/01xbh9923","country_code":"CN","type":"education","lineage":["https://openalex.org/I4405279728"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yuanxiang Liu","raw_affiliation_strings":["Qilu Medical University , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Qilu Medical University , , ,","institution_ids":["https://openalex.org/I4405279728"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5136404464","display_name":"Ziyuan Ye","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405279728","display_name":"Qilu Medical University","ror":"https://ror.org/01xbh9923","country_code":"CN","type":"education","lineage":["https://openalex.org/I4405279728"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ziyuan Ye","raw_affiliation_strings":["Qilu Medical University , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Qilu Medical University , , ,","institution_ids":["https://openalex.org/I4405279728"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087543440","display_name":"Weitong Cui","orcid":"https://orcid.org/0000-0001-9083-0494","observed_orcids":["https://orcid.org/0000-0001-9083-0494"]},"institutions":[{"id":"https://openalex.org/I4405279728","display_name":"Qilu Medical University","ror":"https://ror.org/01xbh9923","country_code":"CN","type":"education","lineage":["https://openalex.org/I4405279728"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Weitong Cui","raw_affiliation_strings":["Qilu Medical University , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Qilu Medical University , , ,","institution_ids":["https://openalex.org/I4405279728"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161454451","display_name":"Xin Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405279728","display_name":"Qilu Medical University","ror":"https://ror.org/01xbh9923","country_code":"CN","type":"education","lineage":["https://openalex.org/I4405279728"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xin Li","raw_affiliation_strings":["Qilu Medical University , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Qilu Medical University , , ,","institution_ids":["https://openalex.org/I4405279728"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031368188","display_name":"Yishu Peng","orcid":"https://orcid.org/0000-0003-3942-978X","observed_orcids":["https://orcid.org/0000-0003-3942-978X"]},"institutions":[{"id":"https://openalex.org/I154099455","display_name":"Shandong University","ror":"https://ror.org/0207yh398","country_code":"CN","type":"education","lineage":["https://openalex.org/I154099455"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yishu Peng","raw_affiliation_strings":["Shandong University , , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shandong University , , , ,","institution_ids":["https://openalex.org/I154099455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161445640","display_name":"Wei Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I154099455","display_name":"Shandong University","ror":"https://ror.org/0207yh398","country_code":"CN","type":"education","lineage":["https://openalex.org/I154099455"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wei Wang","raw_affiliation_strings":["Shandong University , , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shandong University , , , ,","institution_ids":["https://openalex.org/I154099455"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035608376","display_name":"Zhang Ye","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74485261","display_name":"Shandong Agricultural University","ror":"https://ror.org/02ke8fw32","country_code":"CN","type":"education","lineage":["https://openalex.org/I74485261"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ye Zhang","raw_affiliation_strings":["Shandong Agricultural University , , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shandong Agricultural University , , , ,","institution_ids":["https://openalex.org/I74485261"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055428913","display_name":"Chunyu Yang","orcid":"https://orcid.org/0000-0002-4202-9325","observed_orcids":["https://orcid.org/0000-0002-4202-9325","https://orcid.org/0000-0001-9838-0512"]},"institutions":[{"id":"https://openalex.org/I4405279728","display_name":"Qilu Medical University","ror":"https://ror.org/01xbh9923","country_code":"CN","type":"education","lineage":["https://openalex.org/I4405279728"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Lining Wang","raw_affiliation_strings":["Qilu Medical University , , ,"],"raw_orcid":"https://orcid.org/0000-0001-9838-0512","affiliations":[{"raw_affiliation_string":"Qilu Medical University , , ,","institution_ids":["https://openalex.org/I4405279728"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5055428913"],"corresponding_institution_ids":["https://openalex.org/I4405279728"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11979","display_name":"Garlic and Onion Studies","score":0.7836999893188477,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11979","display_name":"Garlic and Onion Studies","score":0.7836999893188477,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.1639000028371811,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.016300000250339508,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6978999972343445},{"id":"https://openalex.org/C2777289219","wikidata":"https://www.wikidata.org/wiki/Q7632154","display_name":"Substrate (aquarium)","level":2,"score":0.6172000169754028},{"id":"https://openalex.org/C2781005338","wikidata":"https://www.wikidata.org/wiki/Q409641","display_name":"Allicin","level":2,"score":0.6013000011444092},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.586899995803833},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5152000188827515},{"id":"https://openalex.org/C69118441","wikidata":"https://www.wikidata.org/wiki/Q2941663","display_name":"Enzyme catalysis","level":3,"score":0.5105000138282776},{"id":"https://openalex.org/C114373084","wikidata":"https://www.wikidata.org/wiki/Q273499","display_name":"Chemical synthesis","level":3,"score":0.5027999877929688},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.4880000054836273},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.40610000491142273},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3849000036716461},{"id":"https://openalex.org/C16525657","wikidata":"https://www.wikidata.org/wiki/Q5201183","display_name":"Cystathionine beta synthase","level":4,"score":0.37880000472068787},{"id":"https://openalex.org/C25366896","wikidata":"https://www.wikidata.org/wiki/Q422785","display_name":"Organosulfur compounds","level":3,"score":0.3709000051021576},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.3653999865055084},{"id":"https://openalex.org/C198503264","wikidata":"https://www.wikidata.org/wiki/Q471635","display_name":"Amination","level":3,"score":0.3637999892234802},{"id":"https://openalex.org/C2780859938","wikidata":"https://www.wikidata.org/wiki/Q3065731","display_name":"Pyridoxal","level":3,"score":0.3594000041484833},{"id":"https://openalex.org/C2778448320","wikidata":"https://www.wikidata.org/wiki/Q408395","display_name":"Sulfoxide","level":2,"score":0.35910001397132874},{"id":"https://openalex.org/C171852809","wikidata":"https://www.wikidata.org/wiki/Q731154","display_name":"Chemical biology","level":2,"score":0.34369999170303345},{"id":"https://openalex.org/C2776834122","wikidata":"https://www.wikidata.org/wiki/Q418957","display_name":"Pyridoxal phosphate","level":4,"score":0.3409999907016754},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.3384999930858612},{"id":"https://openalex.org/C181647583","wikidata":"https://www.wikidata.org/wiki/Q903429","display_name":"Stereoselectivity","level":3,"score":0.3370000123977661},{"id":"https://openalex.org/C2778452553","wikidata":"https://www.wikidata.org/wiki/Q407927","display_name":"Dimethyl sulfoxide","level":2,"score":0.3151000142097473},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.3125999867916107},{"id":"https://openalex.org/C65604355","wikidata":"https://www.wikidata.org/wiki/Q3523861","display_name":"Molecular engineering","level":2,"score":0.3075000047683716},{"id":"https://openalex.org/C2777269803","wikidata":"https://www.wikidata.org/wiki/Q407727","display_name":"Lyase","level":3,"score":0.3019999861717224},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.2987000048160553},{"id":"https://openalex.org/C191503008","wikidata":"https://www.wikidata.org/wiki/Q16007026","display_name":"Biocatalysis","level":4,"score":0.2939000129699707},{"id":"https://openalex.org/C129694887","wikidata":"https://www.wikidata.org/wiki/Q47455153","display_name":"Stereoisomerism","level":3,"score":0.27790001034736633}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1021/acs.jafc.6c13632","doi":"https://doi.org/10.1021/acs.jafc.6c13632","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.jafc.6c13632","pdf_url":null,"source":{"id":"https://openalex.org/S134644764","display_name":"Journal of Agricultural and Food Chemistry","issn_l":"0021-8561","issn":["0021-8561","1520-5118"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Agricultural and Food Chemistry","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":160,"referenced_works":["https://openalex.org/W87506139","https://openalex.org/W1508934482","https://openalex.org/W1599933116","https://openalex.org/W1810581179","https://openalex.org/W1829598765","https://openalex.org/W1922271780","https://openalex.org/W1928032311","https://openalex.org/W1964099296","https://openalex.org/W1964366874","https://openalex.org/W1970264454","https://openalex.org/W1970425655","https://openalex.org/W1972780101","https://openalex.org/W1974544280","https://openalex.org/W1976477725","https://openalex.org/W1976791458","https://openalex.org/W1978318979","https://openalex.org/W1978769714","https://openalex.org/W1982406060","https://openalex.org/W1983729946","https://openalex.org/W1983804112","https://openalex.org/W1984521544","https://openalex.org/W1995589108","https://openalex.org/W1996196679","https://openalex.org/W2003262216","https://openalex.org/W2011513747","https://openalex.org/W2011763359","https://openalex.org/W2014445031","https://openalex.org/W2016648101","https://openalex.org/W2018978350","https://openalex.org/W2020453834","https://openalex.org/W2022415435","https://openalex.org/W2025665147","https://openalex.org/W2031516753","https://openalex.org/W2031654513","https://openalex.org/W2035943254","https://openalex.org/W2036813692","https://openalex.org/W2037525379","https://openalex.org/W2039702265","https://openalex.org/W2041882724","https://openalex.org/W2045808714","https://openalex.org/W2048204704","https://openalex.org/W2058405767","https://openalex.org/W2058438709","https://openalex.org/W2064551081","https://openalex.org/W2066040924","https://openalex.org/W2066370200","https://openalex.org/W2071568711","https://openalex.org/W2076111793","https://openalex.org/W2077103701","https://openalex.org/W2078757278","https://openalex.org/W2085472819","https://openalex.org/W2085893695","https://openalex.org/W2091970515","https://openalex.org/W2101092776","https://openalex.org/W2101947110","https://openalex.org/W2110094991","https://openalex.org/W2115475928","https://openalex.org/W2117834127","https://openalex.org/W2123132123","https://openalex.org/W2129993012","https://openalex.org/W2138012438","https://openalex.org/W2139680667","https://openalex.org/W2143060525","https://openalex.org/W2152065168","https://openalex.org/W2157947948","https://openalex.org/W2165296968","https://openalex.org/W2229134324","https://openalex.org/W2229299714","https://openalex.org/W2234497665","https://openalex.org/W2345114863","https://openalex.org/W2407712884","https://openalex.org/W2438537421","https://openalex.org/W2466120442","https://openalex.org/W2514614044","https://openalex.org/W2625579297","https://openalex.org/W2726801258","https://openalex.org/W2748328505","https://openalex.org/W2753593237","https://openalex.org/W2766237759","https://openalex.org/W2767604394","https://openalex.org/W2772841410","https://openalex.org/W2799343390","https://openalex.org/W2804040157","https://openalex.org/W2804801563","https://openalex.org/W2902148700","https://openalex.org/W2907581332","https://openalex.org/W2911872747","https://openalex.org/W2920573059","https://openalex.org/W2925296528","https://openalex.org/W2938119160","https://openalex.org/W2944120872","https://openalex.org/W2945897269","https://openalex.org/W2947105626","https://openalex.org/W2963773656","https://openalex.org/W2964771029","https://openalex.org/W2974891919","https://openalex.org/W2981642487","https://openalex.org/W2996986155","https://openalex.org/W2997711059","https://openalex.org/W3037532843","https://openalex.org/W3048866389","https://openalex.org/W3084057161","https://openalex.org/W3089762215","https://openalex.org/W3093837539","https://openalex.org/W3107994315","https://openalex.org/W3156493549","https://openalex.org/W3183591370","https://openalex.org/W3195087509","https://openalex.org/W3202426485","https://openalex.org/W4214916360","https://openalex.org/W4224949122","https://openalex.org/W4250950851","https://openalex.org/W4282576500","https://openalex.org/W4292559953","https://openalex.org/W4306250210","https://openalex.org/W4307706834","https://openalex.org/W4311380049","https://openalex.org/W4313339790","https://openalex.org/W4313416499","https://openalex.org/W4313472133","https://openalex.org/W4319842310","https://openalex.org/W4360823659","https://openalex.org/W4376275786","https://openalex.org/W4376601927","https://openalex.org/W4377223733","https://openalex.org/W4382060579","https://openalex.org/W4382137832","https://openalex.org/W4384207237","https://openalex.org/W4388526549","https://openalex.org/W4390718970","https://openalex.org/W4391544240","https://openalex.org/W4391755104","https://openalex.org/W4393342758","https://openalex.org/W4396721167","https://openalex.org/W4399116899","https://openalex.org/W4399141273","https://openalex.org/W4399459012","https://openalex.org/W4400535551","https://openalex.org/W4403581021","https://openalex.org/W4404437410","https://openalex.org/W4405010693","https://openalex.org/W4405263776","https://openalex.org/W4405609184","https://openalex.org/W4405869563","https://openalex.org/W4406124643","https://openalex.org/W4407141900","https://openalex.org/W4407394592","https://openalex.org/W4408994891","https://openalex.org/W4409190486","https://openalex.org/W4413806304","https://openalex.org/W4414156552","https://openalex.org/W4414169548","https://openalex.org/W4414657020","https://openalex.org/W4416903785","https://openalex.org/W4416937620","https://openalex.org/W7119209832","https://openalex.org/W7126398753","https://openalex.org/W7128531363","https://openalex.org/W7134198815","https://openalex.org/W7154692147"],"related_works":[],"abstract_inverted_index":{"Abstract":[0],"Thiosulfinates,":[1],"represented":[2],"by":[3,68],"diallyl":[4],"thiosulfinate":[5,95,100],"(allicin),":[6],"are":[7,66],"the":[8,12,104,114],"material":[9],"basis":[10],"of":[11,17,111,116],"characteristic":[13],"flavor":[14],"and":[15,26,38,71,102,106,113,125,136,143],"bioactivity":[16],"Allium":[18],"plants.":[19],"They":[20],"show":[21],"broad-spectrum":[22],"antibacterial,":[23],"antibiofilm,":[24],"antiviral,":[25],"antitumor":[27],"activities.":[28],"However,":[29],"their":[30],"extreme":[31],"chemical":[32,39],"instability":[33],"challenges":[34],"conventional":[35],"plant":[36],"extraction":[37,70],"synthesis.":[40],"In":[41],"situ":[42],"enzymatic":[43],"synthesis,":[44],"in":[45,133],"which":[46,81],"alliinase":[47],"(l-cysteine":[48],"sulfoxide":[49,58],"lyase,":[50],"CSL)":[51],"generates":[52],"thiosulfinates":[53],"on":[54],"demand":[55],"from":[56],"S-alk(en)yl-l-cysteine":[57],"substrates,":[59],"offers":[60],"a":[61,91],"new":[62],"solution.":[63],"Plant-derived":[64],"CSLs":[65],"limited":[67],"difficult":[69],"low":[72,86],"heterologous":[73],"expression":[74],"efficiency,":[75],"whereas":[76],"bacterial":[77],"cystathionine":[78],"\u03b2-lyase":[79],"(CBL),":[80],"is":[82],"easily":[83],"expressed":[84],"at":[85,121],"cost,":[87],"has":[88],"emerged":[89],"as":[90],"novel":[92],"catalyst":[93],"for":[94],"biosynthesis.":[96],"This":[97],"review":[98],"summarizes":[99],"chemistry":[101],"biosynthesis,":[103],"structure":[105],"pyridoxal":[107],"phosphate":[108],"(PLP)-dependent":[109],"catalysis":[110],"CBL,":[112],"discovery":[115],"CBL\u2019s":[117,131],"CSL":[118],"activity.":[119],"Aiming":[120],"future":[122],"food,":[123],"agricultural,":[124],"pharmaceutical":[126],"applications,":[127],"we":[128],"further":[129],"analyze":[130],"bottlenecks":[132],"substrate":[134],"stereoselectivity":[135],"pH":[137],"preference,":[138],"highlighting":[139],"enzyme":[140],"resource":[141],"mining":[142],"molecular":[144],"engineering":[145],"strategies.":[146]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2026-11-12T00:00:00"}
