{"id":"https://openalex.org/W7225821005","doi":"https://doi.org/10.64898/2026.11.08.765200","title":"An architectural RNA builds a reaction crucible through molecular concentration and activated enzyme selection","display_name":"An architectural RNA builds a reaction crucible through molecular concentration and activated enzyme selection","publication_year":2026,"publication_date":"2026-11-11","ids":{"openalex":"https://openalex.org/W7225821005","doi":"https://doi.org/10.64898/2026.11.08.765200"},"language":"en","primary_location":{"id":"doi:10.64898/2026.11.08.765200","doi":"https://doi.org/10.64898/2026.11.08.765200","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.08.765200","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.11.08.765200","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5133649950","display_name":"Tsuyoshi Ueno","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tsuyoshi Ueno","raw_affiliation_strings":["The Universiy of Osaka;"],"raw_orcid":"https://orcid.org/0009-0003-9474-9249","affiliations":[{"raw_affiliation_string":"The Universiy of Osaka;","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082046242","display_name":"Shungo Adachi","orcid":"https://orcid.org/0000-0001-5232-411X","observed_orcids":["https://orcid.org/0000-0001-5232-411X"]},"institutions":[{"id":"https://openalex.org/I4405287541","display_name":"National Cancer Center Research Institute","ror":"https://ror.org/05fd4mq57","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210087348","https://openalex.org/I4405287541"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shungo Adachi","raw_affiliation_strings":["National Cancer Center Research Institute;"],"raw_orcid":"https://orcid.org/0000-0001-5232-411X","affiliations":[{"raw_affiliation_string":"National Cancer Center Research Institute;","institution_ids":["https://openalex.org/I4405287541"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043066371","display_name":"Tomohiro Yamazaki","orcid":"https://orcid.org/0000-0003-0866-5173","observed_orcids":["https://orcid.org/0000-0003-0866-5173"]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tomohiro Yamazaki","raw_affiliation_strings":["The University of Osaka"],"raw_orcid":"https://orcid.org/0000-0003-0866-5173","affiliations":[{"raw_affiliation_string":"The University of Osaka","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113829574","display_name":"Kensuke Ninomiya","orcid":"https://orcid.org/0000-0002-9395-369X","observed_orcids":["https://orcid.org/0000-0002-9395-369X"]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kensuke Ninomiya","raw_affiliation_strings":["The University of Osaka"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Osaka","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5038000673","display_name":"Tetsuro Hirose","orcid":"https://orcid.org/0000-0003-1068-5464","observed_orcids":["https://orcid.org/0000-0003-1068-5464"]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Tetsuro Hirose","raw_affiliation_strings":["The University of Osaka"],"raw_orcid":"https://orcid.org/0000-0003-1068-5464","affiliations":[{"raw_affiliation_string":"The University of Osaka","institution_ids":["https://openalex.org/I98285908"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5038000673"],"corresponding_institution_ids":["https://openalex.org/I98285908"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.5823000073432922},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.5701000094413757},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5659999847412109},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5102999806404114},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.47769999504089355},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4758000075817108},{"id":"https://openalex.org/C81917197","wikidata":"https://www.wikidata.org/wiki/Q628760","display_name":"Selection (genetic algorithm)","level":2,"score":0.42640000581741333},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3937999904155731},{"id":"https://openalex.org/C168240541","wikidata":"https://www.wikidata.org/wiki/Q45948","display_name":"Organelle","level":2,"score":0.3871000111103058},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.35920000076293945},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.35030001401901245},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3264000117778778},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.3000999987125397},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.2962000072002411},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.29159998893737793},{"id":"https://openalex.org/C100631289","wikidata":"https://www.wikidata.org/wiki/Q898362","display_name":"Posttranslational modification","level":3,"score":0.2858000099658966},{"id":"https://openalex.org/C120500152","wikidata":"https://www.wikidata.org/wiki/Q5189793","display_name":"Crucible (geodemography)","level":2,"score":0.28279998898506165},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2806999981403351},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.27869999408721924},{"id":"https://openalex.org/C89429830","wikidata":"https://www.wikidata.org/wiki/Q735710","display_name":"Scaffold","level":2,"score":0.2759999930858612},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.2671000063419342},{"id":"https://openalex.org/C2991944050","wikidata":"https://www.wikidata.org/wiki/Q36534","display_name":"Reaction conditions","level":3,"score":0.26179999113082886},{"id":"https://openalex.org/C97029542","wikidata":"https://www.wikidata.org/wiki/Q281417","display_name":"Protein kinase A","level":3,"score":0.2590999901294708},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.25029999017715454}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.11.08.765200","doi":"https://doi.org/10.64898/2026.11.08.765200","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.08.765200","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.11.08.765200","doi":"https://doi.org/10.64898/2026.11.08.765200","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.08.765200","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Architectural":[0],"RNAs":[1],"(arcRNAs)":[2],"scaffold":[3],"membraneless":[4],"organelles":[5],"(MLOs),":[6],"yet":[7],"how":[8,131],"they":[9],"organize":[10,133],"functional":[11,134],"proteins":[12,135],"remains":[13],"unclear.":[14],"Nuclear":[15],"stress":[16,29],"bodies":[17],"(nSBs),":[18],"MLOs":[19],"scaffolded":[20],"by":[21,84],"HSATIII":[22,116],"arcRNAs,":[23],"incorporate":[24],"SRSFs":[25,66,86],"during":[26],"stress.":[27],"During":[28],"recovery,":[30],"nSBs":[31],"function":[32,125],"as":[33,55,98],"reaction":[34,96],"crucibles":[35,97],"that":[36,59,78,114],"recruit":[37,67],"the":[38,52],"kinase":[39],"CLK1":[40,73,88],"to":[41,122,142],"accelerate":[42],"SRSF":[43,82],"rephosphorylation":[44],"and":[45,87,101,111,139],"normalize":[46],"stress-induced":[47],"splicing.":[48],"Here,":[49],"we":[50],"identify":[51],"ASAP":[53],"complex":[54],"a":[56,70],"recruitment":[57],"factor":[58],"recognizes":[60],"Ser341-phosphorylated,":[61],"catalytically":[62],"activated":[63,92],"CLK1.":[64],"HSATIII-bound":[65],"ASAP,":[68],"assembling":[69],"platform":[71,80],"for":[72],"recruitment.":[74],"Biochemical":[75],"reconstitution":[76],"reveals":[77],"this":[79,119],"enhances":[81],"phosphorylation":[83],"concentrating":[85],"while":[89],"selectively":[90],"recruiting":[91],"CLK1,":[93],"establishing":[94],"nSB":[95],"molecular":[99],"concentrators":[100],"selectors.":[102],"The":[103],"SRSF-ASAP-CLK1":[104],"interaction":[105],"network":[106,121],"is":[107],"conserved":[108],"between":[109],"humans":[110],"mice,":[112],"suggesting":[113],"primate-specific":[115],"arcRNAs":[117,132],"co-opted":[118],"pre-existing":[120],"establish":[123],"reaction-crucible":[124],"in":[126],"nSBs.":[127],"Our":[128],"findings":[129],"reveal":[130],"through":[136],"quantitative":[137],"enrichment":[138],"qualitative":[140],"selection":[141],"build":[143],"biochemically":[144],"active":[145],"MLOs.":[146]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2026-11-12T00:00:00"}