{"id":"https://openalex.org/W7225820578","doi":"https://doi.org/10.21203/rs.3.rs-11258627/v1","title":"Lactic Acid Bacterial Consortia Modulate Fermentation and Microbial Communities in a Dynamic in Vitro Ruminant Gastrointestinal Model","display_name":"Lactic Acid Bacterial Consortia Modulate Fermentation and Microbial Communities in a Dynamic in Vitro Ruminant Gastrointestinal Model","publication_year":2026,"publication_date":"2026-11-11","ids":{"openalex":"https://openalex.org/W7225820578","doi":"https://doi.org/10.21203/rs.3.rs-11258627/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-11258627/v1","doi":"https://doi.org/10.21203/rs.3.rs-11258627/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11258627/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.21203/rs.3.rs-11258627/v1","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059095515","display_name":"Barbara P\u0142acheta-Kwiatkowska","orcid":"https://orcid.org/0000-0002-3256-456X","observed_orcids":["https://orcid.org/0000-0002-3256-456X"]},"institutions":[{"id":"https://openalex.org/I188884621","display_name":"Lodz University of Technology","ror":"https://ror.org/00s8fpf52","country_code":"PL","type":"education","lineage":["https://openalex.org/I188884621"]}],"countries":["PL"],"is_corresponding":true,"raw_author_name":"Barbara P\u0142acheta-Kwiatkowska","raw_affiliation_strings":["Lodz University of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lodz University of Technology","institution_ids":["https://openalex.org/I188884621"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083715319","display_name":"Ilona Motyl","orcid":"https://orcid.org/0000-0002-7495-5315","observed_orcids":["https://orcid.org/0000-0002-7495-5315"]},"institutions":[{"id":"https://openalex.org/I188884621","display_name":"Lodz University of Technology","ror":"https://ror.org/00s8fpf52","country_code":"PL","type":"education","lineage":["https://openalex.org/I188884621"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Ilona Motyl","raw_affiliation_strings":["Lodz University of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lodz University of Technology","institution_ids":["https://openalex.org/I188884621"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5130606900","display_name":"Ma\u0142gorzata Wla\u017alak","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I188884621","display_name":"Lodz University of Technology","ror":"https://ror.org/00s8fpf52","country_code":"PL","type":"education","lineage":["https://openalex.org/I188884621"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Ma\u0142gorzata Wla\u017alak","raw_affiliation_strings":["Lodz University of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lodz University of Technology","institution_ids":["https://openalex.org/I188884621"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004197712","display_name":"Agnieszka Wilkowska","orcid":"https://orcid.org/0000-0003-1675-4387","observed_orcids":["https://orcid.org/0000-0003-1675-4387"]},"institutions":[{"id":"https://openalex.org/I188884621","display_name":"Lodz University of Technology","ror":"https://ror.org/00s8fpf52","country_code":"PL","type":"education","lineage":["https://openalex.org/I188884621"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Agnieszka Wilkowska","raw_affiliation_strings":["Lodz University of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lodz University of Technology","institution_ids":["https://openalex.org/I188884621"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081403998","display_name":"Tomasz Ruman","orcid":"https://orcid.org/0000-0002-9899-8627","observed_orcids":["https://orcid.org/0000-0002-9899-8627"]},"institutions":[{"id":"https://openalex.org/I111664937","display_name":"Rzesz\u00f3w University of Technology","ror":"https://ror.org/056xse072","country_code":"PL","type":"education","lineage":["https://openalex.org/I111664937"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Tomasz Ruman","raw_affiliation_strings":["Rzesz\u00f3w University of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Rzesz\u00f3w University of Technology","institution_ids":["https://openalex.org/I111664937"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026900150","display_name":"Joanna Nizio\u0142","orcid":"https://orcid.org/0000-0002-4783-8615","observed_orcids":["https://orcid.org/0000-0002-4783-8615"]},"institutions":[{"id":"https://openalex.org/I111664937","display_name":"Rzesz\u00f3w University of Technology","ror":"https://ror.org/056xse072","country_code":"PL","type":"education","lineage":["https://openalex.org/I111664937"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Joanna Nizio\u0142","raw_affiliation_strings":["Rzesz\u00f3w University of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Rzesz\u00f3w University of Technology","institution_ids":["https://openalex.org/I111664937"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038782523","display_name":"Grzegorz Be\u0142\u017cecki","orcid":"https://orcid.org/0000-0003-2237-1454","observed_orcids":["https://orcid.org/0000-0003-2237-1454"]},"institutions":[{"id":"https://openalex.org/I4210100131","display_name":"Kielanowski Institute of Animal Physiology and Nutrition","ror":"https://ror.org/01270hm15","country_code":"PL","type":"facility","lineage":["https://openalex.org/I4210100131","https://openalex.org/I99542240"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Grzegorz Be\u0142\u017cecki","raw_affiliation_strings":["Kielanowski Institute of Animal Physiology and Nutrition"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kielanowski Institute of Animal Physiology and Nutrition","institution_ids":["https://openalex.org/I4210100131"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5017127396","display_name":"Joanna Ber\u0142owska","orcid":"https://orcid.org/0000-0002-9465-4819","observed_orcids":["https://orcid.org/0000-0002-9465-4819"]},"institutions":[{"id":"https://openalex.org/I188884621","display_name":"Lodz University of Technology","ror":"https://ror.org/00s8fpf52","country_code":"PL","type":"education","lineage":["https://openalex.org/I188884621"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Joanna Ber\u0142owska","raw_affiliation_strings":["Lodz University of Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lodz University of Technology","institution_ids":["https://openalex.org/I188884621"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5059095515"],"corresponding_institution_ids":["https://openalex.org/I188884621"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10098","display_name":"Ruminant Nutrition and Digestive Physiology","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10098","display_name":"Ruminant Nutrition and Digestive Physiology","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10152","display_name":"Animal Nutrition and Physiology","score":0.002199999988079071,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C70262065","wikidata":"https://www.wikidata.org/wiki/Q427652","display_name":"Rumen","level":3,"score":0.839900016784668},{"id":"https://openalex.org/C2781003712","wikidata":"https://www.wikidata.org/wiki/Q842175","display_name":"Ruminant","level":3,"score":0.8029000163078308},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.7778000235557556},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.6668000221252441},{"id":"https://openalex.org/C2775920511","wikidata":"https://www.wikidata.org/wiki/Q161249","display_name":"Lactic acid","level":3,"score":0.6575999855995178},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6439999938011169},{"id":"https://openalex.org/C2776544680","wikidata":"https://www.wikidata.org/wiki/Q660071","display_name":"Silage","level":2,"score":0.5302000045776367},{"id":"https://openalex.org/C15151743","wikidata":"https://www.wikidata.org/wiki/Q903778","display_name":"Metagenomics","level":3,"score":0.5174999833106995},{"id":"https://openalex.org/C2781357212","wikidata":"https://www.wikidata.org/wiki/Q5275608","display_name":"Digestion (alchemy)","level":2,"score":0.5091000199317932},{"id":"https://openalex.org/C543025807","wikidata":"https://www.wikidata.org/wiki/Q61476","display_name":"Fatty acid","level":2,"score":0.4749999940395355},{"id":"https://openalex.org/C81407943","wikidata":"https://www.wikidata.org/wiki/Q17149178","display_name":"Microbial population biology","level":3,"score":0.4426000118255615},{"id":"https://openalex.org/C2780512811","wikidata":"https://www.wikidata.org/wiki/Q11829360","display_name":"Gastrointestinal tract","level":2,"score":0.41659998893737793},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4068000018596649},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3968000113964081},{"id":"https://openalex.org/C2780437316","wikidata":"https://www.wikidata.org/wiki/Q578677","display_name":"Lactobacillaceae","level":4,"score":0.3837999999523163},{"id":"https://openalex.org/C2775980262","wikidata":"https://www.wikidata.org/wiki/Q218870","display_name":"Prebiotic","level":2,"score":0.37689998745918274},{"id":"https://openalex.org/C101014631","wikidata":"https://www.wikidata.org/wiki/Q4889568","display_name":"Microbial metabolism","level":3,"score":0.3481000065803528},{"id":"https://openalex.org/C176221332","wikidata":"https://www.wikidata.org/wiki/Q43017","display_name":"Lactic acid fermentation","level":4,"score":0.34709998965263367},{"id":"https://openalex.org/C2781092032","wikidata":"https://www.wikidata.org/wiki/Q327663","display_name":"Clostridium","level":3,"score":0.34380000829696655},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3424000144004822},{"id":"https://openalex.org/C135870905","wikidata":"https://www.wikidata.org/wiki/Q903667","display_name":"Metabolome","level":3,"score":0.3366999924182892},{"id":"https://openalex.org/C175605896","wikidata":"https://www.wikidata.org/wiki/Q39833","display_name":"Microorganism","level":3,"score":0.33230000734329224},{"id":"https://openalex.org/C147490202","wikidata":"https://www.wikidata.org/wiki/Q5465528","display_name":"Food spoilage","level":3,"score":0.3310999870300293},{"id":"https://openalex.org/C2778312467","wikidata":"https://www.wikidata.org/wiki/Q132809","display_name":"Clostridia","level":3,"score":0.32089999318122864},{"id":"https://openalex.org/C2777306163","wikidata":"https://www.wikidata.org/wiki/Q2986869","display_name":"Microbial consortium","level":4,"score":0.3140000104904175},{"id":"https://openalex.org/C509243982","wikidata":"https://www.wikidata.org/wiki/Q2836947","display_name":"Animal feed","level":2,"score":0.3061999976634979},{"id":"https://openalex.org/C2780027724","wikidata":"https://www.wikidata.org/wiki/Q288892","display_name":"Ruminococcus","level":3,"score":0.3003000020980835},{"id":"https://openalex.org/C143121216","wikidata":"https://www.wikidata.org/wiki/Q1330402","display_name":"Microbiome","level":2,"score":0.2955000102519989},{"id":"https://openalex.org/C121677567","wikidata":"https://www.wikidata.org/wiki/Q73425","display_name":"Microbial inoculant","level":3,"score":0.2930999994277954},{"id":"https://openalex.org/C21565614","wikidata":"https://www.wikidata.org/wiki/Q12149006","display_name":"Metabolomics","level":2,"score":0.28769999742507935},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2865000069141388},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.28290000557899475},{"id":"https://openalex.org/C2775944082","wikidata":"https://www.wikidata.org/wiki/Q27004","display_name":"Abomasum","level":4,"score":0.26820001006126404},{"id":"https://openalex.org/C2780206646","wikidata":"https://www.wikidata.org/wiki/Q1061596","display_name":"Lactobacillus","level":3,"score":0.2581999897956848},{"id":"https://openalex.org/C69562835","wikidata":"https://www.wikidata.org/wiki/Q3054637","display_name":"Microbial ecology","level":3,"score":0.2558000087738037},{"id":"https://openalex.org/C2780255968","wikidata":"https://www.wikidata.org/wiki/Q1816730","display_name":"Probiotic","level":3,"score":0.25099998712539673}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-11258627/v1","doi":"https://doi.org/10.21203/rs.3.rs-11258627/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11258627/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-11258627/v1","doi":"https://doi.org/10.21203/rs.3.rs-11258627/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11258627/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":40,"referenced_works":["https://openalex.org/W1842963151","https://openalex.org/W1876041487","https://openalex.org/W1878521557","https://openalex.org/W1892731140","https://openalex.org/W1947953472","https://openalex.org/W1964173392","https://openalex.org/W1987100560","https://openalex.org/W1997319125","https://openalex.org/W2036146859","https://openalex.org/W2036897871","https://openalex.org/W2059899429","https://openalex.org/W2061799513","https://openalex.org/W2103901746","https://openalex.org/W2107772251","https://openalex.org/W2112364185","https://openalex.org/W2408328900","https://openalex.org/W2432815617","https://openalex.org/W2600083889","https://openalex.org/W2613065664","https://openalex.org/W2789386374","https://openalex.org/W2914485192","https://openalex.org/W2938868597","https://openalex.org/W2950464960","https://openalex.org/W3036044218","https://openalex.org/W3036135788","https://openalex.org/W4286504262","https://openalex.org/W4294876529","https://openalex.org/W4295929874","https://openalex.org/W4304481015","https://openalex.org/W4380992181","https://openalex.org/W4400136234","https://openalex.org/W4403835683","https://openalex.org/W4406211556","https://openalex.org/W4409229284","https://openalex.org/W4414651914","https://openalex.org/W4415109553","https://openalex.org/W4416419487","https://openalex.org/W7140281540","https://openalex.org/W7163996244","https://openalex.org/W7212040392"],"related_works":[],"abstract_inverted_index":{"Abstract":[0],"Optimization":[1],"of":[2,21,29,59,76,98,189,198],"microbial":[3,46,78,228],"fermentation":[4,50,232],"throughout":[5],"the":[6,27,60,74,77,94,99,152,210,220,238],"ruminant":[7,61],"gastrointestinal":[8,62,241],"tract":[9],"is":[10,247],"important":[11],"for":[12],"efficient":[13],"nutrient":[14],"utilization":[15],"and":[16,42,49,83,118,144,163,173,186,204,213,231],"animal":[17],"health.":[18],"The":[19,64,156],"aim":[20],"this":[22],"study":[23],"was":[24,66,129,180],"to":[25,36,249],"evaluate":[26],"effect":[28],"lactic":[30,164],"acid":[31,165],"bacteria":[32],"(LAB)":[33],"consortia":[34,223],"applied":[35],"a":[37,53],"total":[38],"mixed":[39],"ration":[40],"(TMR)":[41],"maize":[43],"silage":[44],"on":[45,88,103],"community":[47,229],"structure":[48],"profiles":[51,207],"in":[52,55,151,239,244],"dynamic":[54],"vitro":[56,240],"digestion":[57],"system":[58],"tract.":[63],"model":[65],"established":[67],"using":[68],"fresh":[69],"cow":[70],"rumen":[71,105],"fluid":[72],"as":[73],"source":[75],"inoculum.":[79],"Importantly,":[80],"subsequent":[81],"metabolite":[82],"microbiota":[84],"analyses":[85,175],"were":[86,121,149,224],"performed":[87],"samples":[89],"collected":[90,153],"from":[91],"reactors":[92],"representing":[93],"respective":[95],"intestinal":[96],"compartments":[97],"model,":[100],"rather":[101],"than":[102],"donor":[104],"fluid.":[106],"Bacterial":[107],"inoculants":[108],"containing":[109],"Lentilactobacillus":[110,114,116],"buchneri,":[111],"Pediococcus":[112],"acidilactici,":[113],"parakefiri,":[115],"diolivorans":[117],"Furfurilactobacillus":[119],"kisonensis":[120],"tested.":[122],"A":[123],"control":[124],"variant":[125,134,158],"receiving":[126],"non-inoculated":[127],"feed":[128],"compared":[130],"with":[131,136,168,182,201,209,226],"an":[132],"experimental":[133,157,211],"supplemented":[135],"LAB.":[137],"Lactic":[138],"acid,":[139],"short-chain":[140],"fatty":[141,146],"acids":[142,147],"(SCFAs),":[143],"branched-chain":[145],"(BCFAs)":[148],"quantified":[150],"reactor":[154],"samples.":[155],"showed":[159],"higher":[160],"propionate,":[161],"valerate,":[162],"concentrations":[166],"together":[167],"lower":[169],"BCFA":[170],"concentrations.":[171],"Metagenomic":[172],"metabolomic":[174],"indicated":[176],"that":[177,219],"LAB":[178,222],"supplementation":[179],"associated":[181,208,225],"distinct":[183,205],"temporal":[184],"dynamics":[185,233],"greater":[187],"preservation":[188],"selected":[190,199,221],"bacterial":[191],"groups,":[192],"including":[193],"Bacteroidota":[194],",":[195],"reduced":[196],"abundance":[197],"taxa":[200],"opportunistic":[202],"potential,":[203],"metabolic":[206],"treatment":[212],"feeding":[214],"phase.":[215],"These":[216],"findings":[217],"suggest":[218],"altered":[227],"succession":[230],"during":[234],"dietary":[235],"transitions":[236],"within":[237],"ecosystem.":[242],"Further":[243],"vivo":[245],"validation":[246],"required":[248],"determine":[250],"whether":[251],"these":[252],"effects":[253],"are":[254],"reproduced":[255],"under":[256],"physiological":[257],"conditions.":[258]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2026-11-12T00:00:00"}