{"id":"https://openalex.org/W7225808063","doi":"https://doi.org/10.64898/2026.11.10.765695","title":"Denaturation-sensitive BRET probes enable intracellular thermal shift measurements across diverse protein targets and biomolecular complexes","display_name":"Denaturation-sensitive BRET probes enable intracellular thermal shift measurements across diverse protein targets and biomolecular complexes","publication_year":2026,"publication_date":"2026-11-11","ids":{"openalex":"https://openalex.org/W7225808063","doi":"https://doi.org/10.64898/2026.11.10.765695"},"language":"en","primary_location":{"id":"doi:10.64898/2026.11.10.765695","doi":"https://doi.org/10.64898/2026.11.10.765695","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.10.765695","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.11.10.765695","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5019845986","display_name":"James D. Vasta","orcid":"https://orcid.org/0000-0001-8511-2113","observed_orcids":["https://orcid.org/0000-0001-8511-2113"]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James D. Vasta","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":"https://orcid.org/0000-0001-8511-2113","affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015887140","display_name":"Ani Michaud","orcid":"https://orcid.org/0000-0001-9039-3212","observed_orcids":["https://orcid.org/0000-0001-9039-3212"]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ani Michaud","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091779220","display_name":"Michael Beck","orcid":"https://orcid.org/0000-0003-2523-6266","observed_orcids":["https://orcid.org/0000-0003-2523-6266"]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael T. Beck","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5132842289","display_name":"Inhong Hwang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Inhong Hwang","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161449738","display_name":"Ashley Schwarzenstein","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ashley Schwarzenstein","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050845134","display_name":"Cesear R. Corona","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cesear R. Corona","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5147827607","display_name":"Paula Sequeira","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paula Sequeira","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161429112","display_name":"Cara Happe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cara Happe","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051476723","display_name":"Martin P. Schwalm","orcid":"https://orcid.org/0000-0002-1252-1829","observed_orcids":["https://orcid.org/0000-0002-1252-1829"]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Martin P. Schwalm","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161481568","display_name":"Bryan Gehrke","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bryan Gehrke","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161454434","display_name":"Kendra Nealey","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kendra Nealey","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161486612","display_name":"Steven Russell","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Steven Russell","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161463031","display_name":"Bryn Mikulsky","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bryn Mikulsky","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101232599","display_name":"Kaitlin K. Dunn Hoffman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kaitlin K. Dunn-Hoffman","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5129672889","display_name":"Cameron Damgaard","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cameron Damgaard","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161493612","display_name":"Min Zhou","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Min Zhou","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071768059","display_name":"Sophia Berry","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sophia Berry","raw_affiliation_strings":["AstraZeneca;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AstraZeneca;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076127269","display_name":"Laura E J Usselmann","orcid":"https://orcid.org/0000-0003-1429-4065","observed_orcids":["https://orcid.org/0000-0003-1429-4065"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Laura Usselmann","raw_affiliation_strings":["AstraZeneca;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AstraZeneca;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161439476","display_name":"Calum Forbes","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Calum Forbes","raw_affiliation_strings":["AstraZeneca;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AstraZeneca;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001264532","display_name":"Mercedes V\u00e1zquez\u2013Chantada","orcid":"https://orcid.org/0000-0002-5665-2548","observed_orcids":["https://orcid.org/0000-0002-5665-2548"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mercedes Vazquez-Chantada","raw_affiliation_strings":["AstraZeneca;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AstraZeneca;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062888017","display_name":"Thomas B. Lentz","orcid":"https://orcid.org/0000-0001-7710-7744","observed_orcids":["https://orcid.org/0000-0001-7710-7744"]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas B. Lentz","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5134245981","display_name":"Rebecca L Johnson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rebecca L. Johnson","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017408177","display_name":"Michael R. Slater","orcid":"https://orcid.org/0000-0002-2803-0338","observed_orcids":["https://orcid.org/0000-0002-2803-0338"]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael R. Slater","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5114566365","display_name":"Cassidy Ames","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cassidy Ames","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032609690","display_name":"Magdalena M. Szewczyk","orcid":"https://orcid.org/0000-0001-9243-1630","observed_orcids":["https://orcid.org/0000-0001-9243-1630"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Magdalena M. Szewczyk","raw_affiliation_strings":["University of Toronto"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Toronto","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078852954","display_name":"Dalia Barsyte-Lovejoy","orcid":"https://orcid.org/0000-0002-6560-9621","observed_orcids":["https://orcid.org/0000-0002-6560-9621"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Dalia Barsyte-Lovejoy","raw_affiliation_strings":["University of Toronto"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Toronto","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046821772","display_name":"Wenhui Zhou","orcid":"https://orcid.org/0000-0003-2178-3959","observed_orcids":["https://orcid.org/0000-0003-2178-3959"]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wenhui Zhou","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109685876","display_name":"Mei Cong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mei Cong","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002485823","display_name":"Poncho L. Meisenheimer","orcid":"https://orcid.org/0000-0003-1918-3153","observed_orcids":["https://orcid.org/0000-0003-1918-3153"]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Poncho L. Meisenheimer","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5158816681","display_name":"Joseph Shaw","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joseph Shaw","raw_affiliation_strings":["AstraZeneca;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AstraZeneca;","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5032157464","display_name":"Matthew B. Robers","orcid":"https://orcid.org/0000-0001-8505-5227","observed_orcids":["https://orcid.org/0000-0001-8505-5227"]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Matthew B. Robers","raw_affiliation_strings":["Promega Corporation;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation;","institution_ids":["https://openalex.org/I199117387"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.5458999872207642,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.5458999872207642,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13539","display_name":"thermodynamics and calorimetric analyses","score":0.25609999895095825,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12534","display_name":"Protein Degradation and Inhibitors","score":0.0689999982714653,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.61080002784729},{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.5572999715805054},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5547999739646912},{"id":"https://openalex.org/C74187038","wikidata":"https://www.wikidata.org/wiki/Q1418791","display_name":"Drug discovery","level":2,"score":0.45489999651908875},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.43299999833106995},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3734999895095825},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.35740000009536743},{"id":"https://openalex.org/C2989108626","wikidata":"https://www.wikidata.org/wiki/Q904407","display_name":"Drug target","level":2,"score":0.3400000035762787},{"id":"https://openalex.org/C2776923230","wikidata":"https://www.wikidata.org/wiki/Q16955798","display_name":"Denaturation (fissile materials)","level":2,"score":0.3244999945163727},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.3190999925136566},{"id":"https://openalex.org/C2780557392","wikidata":"https://www.wikidata.org/wiki/Q523796","display_name":"Linker","level":2,"score":0.31839999556541443},{"id":"https://openalex.org/C21200559","wikidata":"https://www.wikidata.org/wiki/Q7451068","display_name":"Sensitivity (control systems)","level":2,"score":0.30979999899864197},{"id":"https://openalex.org/C105168689","wikidata":"https://www.wikidata.org/wiki/Q901969","display_name":"Globular protein","level":2,"score":0.295199990272522},{"id":"https://openalex.org/C158711907","wikidata":"https://www.wikidata.org/wiki/Q1768467","display_name":"Fluorescence spectroscopy","level":3,"score":0.29100000858306885},{"id":"https://openalex.org/C183978625","wikidata":"https://www.wikidata.org/wiki/Q903596","display_name":"Electrophoretic mobility shift assay","level":4,"score":0.29100000858306885},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.29010000824928284},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.28360000252723694},{"id":"https://openalex.org/C2780035454","wikidata":"https://www.wikidata.org/wiki/Q8386","display_name":"Drug","level":2,"score":0.2808000147342682},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.2802000045776367},{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.2775999903678894},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.26969999074935913},{"id":"https://openalex.org/C106847996","wikidata":"https://www.wikidata.org/wiki/Q898756","display_name":"Surface plasmon resonance","level":3,"score":0.267300009727478},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.26589998602867126},{"id":"https://openalex.org/C59593255","wikidata":"https://www.wikidata.org/wiki/Q901663","display_name":"Molecular dynamics","level":2,"score":0.2538999915122986}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.11.10.765695","doi":"https://doi.org/10.64898/2026.11.10.765695","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.10.765695","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.11.10.765695","doi":"https://doi.org/10.64898/2026.11.10.765695","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.10.765695","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Thermal":[0],"shift":[1,27,40,61,126],"assays":[2],"have":[3],"revolutionized":[4],"drug":[5,95,151],"discovery,":[6],"but":[7],"can":[8],"be":[9],"limited":[10],"in":[11,49,62,79,112],"sensitivity":[12,119],"and":[13,24,57,109,120,141,148],"scalability.":[14],"Combining":[15],"the":[16,31,35,103,130],"unique":[17],"attributes":[18],"of":[19,102,132],"differential":[20],"scanning":[21],"fluorimetry":[22],"(DSF)":[23],"cellular":[25,81],"thermal":[26,60,125],"(CETSA),":[28],"we":[29],"report":[30],"methodological":[32],"framework":[33],"for":[34,70,89,145],"bioluminescence":[36],"resonance":[37],"energy":[38],"transfer":[39],"assay":[41],"(BRETSA).":[42],"BRETSA":[43,133],"utilizes":[44],"denaturation":[45],"sensitive":[46],"BRET":[47],"probes,":[48],"combination":[50],"with":[51],"luciferase-tagged":[52],"target":[53,77,85,136],"proteins,":[54,74],"to":[55,123,134],"track":[56],"longitudinally":[58],"record":[59],"living":[63],"cells.":[64],"Denaturation":[65],"profiles":[66],"were":[67],"successfully":[68],"measured":[69],"over":[71,90],"500":[72],"globular":[73],"spanning":[75],"24":[76],"families":[78],"diverse":[80],"compartments.":[82],"Among":[83],"these,":[84],"engagement":[86,137],"was":[87],"confirmed":[88],"200":[91],"proteins;":[92],"including":[93],"validated":[94],"targets":[96],"as":[97,99],"well":[98],"understudied":[100],"members":[101],"kinase":[104],"subfamily.":[105],"Leveraging":[106],"orthosteric,":[107],"allosteric,":[108],"covalent":[110],"ligands":[111],"potency":[113],"studies,":[114],"this":[115],"method":[116],"offers":[117],"improved":[118],"scalability":[121],"compared":[122],"other":[124],"methods.":[127],"We":[128],"extend":[129],"utility":[131],"interrogate":[135],"at":[138],"biomolecular":[139],"complexes":[140],"query":[142],"cooperative":[143],"binding":[144],"molecular":[146],"glues":[147],"emerging":[149],"tricomplex":[150],"modalities.":[152]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2026-11-12T00:00:00"}