{"id":"https://openalex.org/W7225804886","doi":"https://doi.org/10.21203/rs.3.rs-11303881/v1","title":"Cofilin1 palmitoylation restrains metastasis by enhancing Ser3 phosphorylation and reducing protein turnover","display_name":"Cofilin1 palmitoylation restrains metastasis by enhancing Ser3 phosphorylation and reducing protein turnover","publication_year":2026,"publication_date":"2026-11-11","ids":{"openalex":"https://openalex.org/W7225804886","doi":"https://doi.org/10.21203/rs.3.rs-11303881/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-11303881/v1","doi":"https://doi.org/10.21203/rs.3.rs-11303881/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11303881/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.21203/rs.3.rs-11303881/v1","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088964081","display_name":"Eryan Kong","orcid":"https://orcid.org/0000-0002-4086-1441","observed_orcids":["https://orcid.org/0000-0002-4086-1441","https://orcid.org/0000-0003-4630-0597"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Eryan Kong","raw_affiliation_strings":["Institute of Psychiatry and Neuroscience"],"raw_orcid":"https://orcid.org/0000-0002-4086-1441","affiliations":[{"raw_affiliation_string":"Institute of Psychiatry and Neuroscience","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019437651","display_name":"Hesheng Kong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139322472","display_name":"Universiti Sains Malaysia","ror":"https://ror.org/02rgb2k63","country_code":"MY","type":"education","lineage":["https://openalex.org/I139322472"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Hesheng Kong","raw_affiliation_strings":["Universiti Sains Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universiti Sains Malaysia","institution_ids":["https://openalex.org/I139322472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088399878","display_name":"Jiangli Zhu","orcid":"https://orcid.org/0000-0002-7467-4484","observed_orcids":["https://orcid.org/0000-0002-7467-4484"]},"institutions":[{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jiangli Zhu","raw_affiliation_strings":["State Key Laboratory of Biotherapy and Cancer Center"],"raw_orcid":"https://orcid.org/0000-0002-7467-4484","affiliations":[{"raw_affiliation_string":"State Key Laboratory of Biotherapy and Cancer Center","institution_ids":["https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161425331","display_name":"Shuang Liu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I80626142","display_name":"Henan Medical University","ror":"https://ror.org/038hzq450","country_code":"CN","type":"education","lineage":["https://openalex.org/I80626142"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shuang Liu","raw_affiliation_strings":["The First Affiliated Hospital of Henan Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The First Affiliated Hospital of Henan Medical University","institution_ids":["https://openalex.org/I80626142"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108335019","display_name":"Birou Lai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139322472","display_name":"Universiti Sains Malaysia","ror":"https://ror.org/02rgb2k63","country_code":"MY","type":"education","lineage":["https://openalex.org/I139322472"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Birou Lai","raw_affiliation_strings":["Universiti Sains Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universiti Sains Malaysia","institution_ids":["https://openalex.org/I139322472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5158478221","display_name":"Shiyi Song","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I80626142","display_name":"Henan Medical University","ror":"https://ror.org/038hzq450","country_code":"CN","type":"education","lineage":["https://openalex.org/I80626142"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shiyi Song","raw_affiliation_strings":["The First Affiliated Hospital of Henan Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The First Affiliated Hospital of Henan Medical University","institution_ids":["https://openalex.org/I80626142"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101996277","display_name":"Jiateng Zhong","orcid":"https://orcid.org/0000-0003-0822-2815","observed_orcids":["https://orcid.org/0000-0003-0822-2815"]},"institutions":[{"id":"https://openalex.org/I80626142","display_name":"Henan Medical University","ror":"https://ror.org/038hzq450","country_code":"CN","type":"education","lineage":["https://openalex.org/I80626142"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jiateng Zhong","raw_affiliation_strings":["Xinxiang Medical University"],"raw_orcid":"https://orcid.org/0000-0003-0822-2815","affiliations":[{"raw_affiliation_string":"Xinxiang Medical University","institution_ids":["https://openalex.org/I80626142"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051546182","display_name":"Tingmin Chang","orcid":"https://orcid.org/0009-0000-8022-4319","observed_orcids":["https://orcid.org/0009-0000-8022-4319"]},"institutions":[{"id":"https://openalex.org/I80626142","display_name":"Henan Medical University","ror":"https://ror.org/038hzq450","country_code":"CN","type":"education","lineage":["https://openalex.org/I80626142"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Tingmin Chang","raw_affiliation_strings":["Xinxiang Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Xinxiang Medical University","institution_ids":["https://openalex.org/I80626142"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161433248","display_name":"Dong Guo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I80626142","display_name":"Henan Medical University","ror":"https://ror.org/038hzq450","country_code":"CN","type":"education","lineage":["https://openalex.org/I80626142"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Dong Guo","raw_affiliation_strings":["The First Affiliated Hospital of Henan Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The First Affiliated Hospital of Henan Medical University","institution_ids":["https://openalex.org/I80626142"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063582426","display_name":"Jian Gao","orcid":"https://orcid.org/0000-0001-6723-0490","observed_orcids":["https://orcid.org/0000-0001-6723-0490"]},"institutions":[{"id":"https://openalex.org/I184681353","display_name":"Anhui University of Science and Technology","ror":"https://ror.org/00q9atg80","country_code":"CN","type":"education","lineage":["https://openalex.org/I184681353"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jian Gao","raw_affiliation_strings":["Anhui University of Science and Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Anhui University of Science and Technology","institution_ids":["https://openalex.org/I184681353"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019202801","display_name":"Badrul Hisham Yahaya","orcid":"https://orcid.org/0000-0002-3295-9676","observed_orcids":["https://orcid.org/0000-0002-3295-9676"]},"institutions":[{"id":"https://openalex.org/I139322472","display_name":"Universiti Sains Malaysia","ror":"https://ror.org/02rgb2k63","country_code":"MY","type":"education","lineage":["https://openalex.org/I139322472"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Badrul Yahaya","raw_affiliation_strings":["Universiti Sains Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universiti Sains Malaysia","institution_ids":["https://openalex.org/I139322472"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5161423083","display_name":"xiao hong kang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I80626142","display_name":"Henan Medical University","ror":"https://ror.org/038hzq450","country_code":"CN","type":"education","lineage":["https://openalex.org/I80626142"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"xiao hong kang","raw_affiliation_strings":["Xinxiang Medical University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Xinxiang Medical University","institution_ids":["https://openalex.org/I80626142"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5088964081"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.4602000117301941,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.4602000117301941,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.3977000117301941,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13437","display_name":"Mechanisms of cancer metastasis","score":0.05820000171661377,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C146727910","wikidata":"https://www.wikidata.org/wiki/Q501620","display_name":"Palmitoylation","level":4,"score":0.7569000124931335},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.717199981212616},{"id":"https://openalex.org/C169822122","wikidata":"https://www.wikidata.org/wiki/Q230187","display_name":"Crosstalk","level":2,"score":0.7113000154495239},{"id":"https://openalex.org/C6929976","wikidata":"https://www.wikidata.org/wiki/Q3771881","display_name":"Regulator","level":3,"score":0.6520000100135803},{"id":"https://openalex.org/C142669718","wikidata":"https://www.wikidata.org/wiki/Q154626","display_name":"Cytoskeleton","level":3,"score":0.5814999938011169},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.569100022315979},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.554099977016449},{"id":"https://openalex.org/C137738243","wikidata":"https://www.wikidata.org/wiki/Q189092","display_name":"Cell migration","level":3,"score":0.5273000001907349},{"id":"https://openalex.org/C2993400109","wikidata":"https://www.wikidata.org/wiki/Q329638","display_name":"Actin cytoskeleton","level":4,"score":0.5067999958992004},{"id":"https://openalex.org/C125705527","wikidata":"https://www.wikidata.org/wiki/Q185269","display_name":"Actin","level":2,"score":0.49869999289512634},{"id":"https://openalex.org/C2779013556","wikidata":"https://www.wikidata.org/wiki/Q181876","display_name":"Metastasis","level":3,"score":0.48410001397132874},{"id":"https://openalex.org/C3620293","wikidata":"https://www.wikidata.org/wiki/Q14908027","display_name":"Actin remodeling","level":5,"score":0.39640000462532043},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.3935999870300293},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.3747999966144562},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.32679998874664307},{"id":"https://openalex.org/C58207958","wikidata":"https://www.wikidata.org/wiki/Q33596","display_name":"Motility","level":2,"score":0.3163999915122986},{"id":"https://openalex.org/C59999672","wikidata":"https://www.wikidata.org/wiki/Q21497084","display_name":"MDia1","level":5,"score":0.3028999865055084},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.29670000076293945},{"id":"https://openalex.org/C2776107976","wikidata":"https://www.wikidata.org/wiki/Q1786433","display_name":"Tumor microenvironment","level":3,"score":0.289900004863739},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.2727000117301941},{"id":"https://openalex.org/C85789140","wikidata":"https://www.wikidata.org/wiki/Q187640","display_name":"Cell adhesion","level":3,"score":0.2718999981880188},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.2718999981880188},{"id":"https://openalex.org/C195580219","wikidata":"https://www.wikidata.org/wiki/Q7251511","display_name":"Protein turnover","level":3,"score":0.26489999890327454},{"id":"https://openalex.org/C16613235","wikidata":"https://www.wikidata.org/wiki/Q285982","display_name":"Endogeny","level":2,"score":0.2572000026702881}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-11303881/v1","doi":"https://doi.org/10.21203/rs.3.rs-11303881/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11303881/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-11303881/v1","doi":"https://doi.org/10.21203/rs.3.rs-11303881/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11303881/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Abstract":[0],"Cofilin1":[1,37,51,122],"is":[2,17],"a":[3,86,117,143],"regulator":[4],"of":[5,24,49,69],"actin":[6,131],"dynamics,":[7],"governing":[8],"cellular":[9],"protrusion,":[10],"migration,":[11],"and":[12,44,57,63,73,98,103,108,124,134],"invasion.":[13],"Although":[14],"its":[15,42,77],"activity":[16],"modulated":[18],"by":[19],"phosphorylation,":[20],"the":[21,66,138],"functional":[22],"relevance":[23],"other":[25],"post-translational":[26,128],"modifications,":[27],"particularly":[28],"palmitoylation,":[29,39],"has":[30],"remained":[31],"unexplored.":[32],"Here,":[33],"we":[34,84],"show":[35],"that":[36],"undergoes":[38],"which":[40],"governs":[41],"stability":[43,123],"Ser3":[45],"phosphorylation.":[46],"Elevated":[47],"levels":[48,97],"palmitoylated":[50],"(palm-Cofilin1)":[52],"correlate":[53],"with":[54,71,92],"reduced":[55],"migratory":[56],"invasive":[58],"capacity.":[59],"We":[60],"identify":[61],"PPT1":[62],"PPT2":[64],"as":[65,76,116],"depalmitoylating":[67],"enzymes":[68],"Cofilin1,":[70],"DHHC1":[72],"DHHC2":[74],"serving":[75],"palmitoylating":[78],"counterparts.":[79],"To":[80],"exploit":[81],"this":[82,93],"regulation,":[83],"developed":[85],"small-molecule":[87],"inhibitor":[88,94],"targeting":[89],"PPT1/2.":[90],"Treatment":[91],"increased":[95],"palm-Cofilin1":[96],"suppressed":[99],"tumor":[100,135,148],"cell":[101],"migration":[102],"invasion":[104],"both":[105],"in":[106,109],"vitro":[107],"vivo.":[110],"Collectively,":[111],"these":[112],"findings":[113],"establish":[114],"palmitoylation":[115],"pivotal":[118],"regulatory":[119],"axis":[120,140],"controlling":[121],"activity,":[125],"thereby":[126],"linking":[127],"crosstalk":[129],"to":[130],"cytoskeleton":[132],"remodeling":[133],"progression.":[136],"Targeting":[137],"PPT1/2\u2013Cofilin1":[139],"therefore":[141],"represents":[142],"compelling":[144],"therapeutic":[145],"strategy":[146],"against":[147],"metastasis.":[149]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2026-11-12T00:00:00"}