{"id":"https://openalex.org/W7225801338","doi":"https://doi.org/10.1021/acsinfecdis.6c00944","title":"A LytA-Derived Biosensor for Identifying Choline-Containing Streptococcal Pathogens","display_name":"A LytA-Derived Biosensor for Identifying Choline-Containing Streptococcal Pathogens","publication_year":2026,"publication_date":"2026-11-11","ids":{"openalex":"https://openalex.org/W7225801338","doi":"https://doi.org/10.1021/acsinfecdis.6c00944"},"language":"en","primary_location":{"id":"doi:10.1021/acsinfecdis.6c00944","doi":"https://doi.org/10.1021/acsinfecdis.6c00944","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acsinfecdis.6c00944","pdf_url":null,"source":{"id":"https://openalex.org/S2764646616","display_name":"ACS Infectious Diseases","issn_l":"2373-8227","issn":["2373-8227"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Infectious Diseases","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5161419120","display_name":"Yijue Wang","orcid":"https://orcid.org/0009-0000-2326-0401","observed_orcids":["https://orcid.org/0009-0000-2326-0401"]},"institutions":[{"id":"https://openalex.org/I37574244","display_name":"Sichuan Agricultural University","ror":"https://ror.org/0388c3403","country_code":"CN","type":"education","lineage":["https://openalex.org/I37574244"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yijue Wang","raw_affiliation_strings":["Sichuan Agricultural University , , ,"],"raw_orcid":"https://orcid.org/0009-0000-2326-0401","affiliations":[{"raw_affiliation_string":"Sichuan Agricultural University , , ,","institution_ids":["https://openalex.org/I37574244"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039231344","display_name":"Jinhuan Liu","orcid":"https://orcid.org/0000-0003-4978-2229","observed_orcids":["https://orcid.org/0000-0003-4978-2229"]},"institutions":[{"id":"https://openalex.org/I37574244","display_name":"Sichuan Agricultural University","ror":"https://ror.org/0388c3403","country_code":"CN","type":"education","lineage":["https://openalex.org/I37574244"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jinhuan Liu","raw_affiliation_strings":["Sichuan Agricultural University , , ,"],"raw_orcid":"https://orcid.org/0000-0003-4978-2229","affiliations":[{"raw_affiliation_string":"Sichuan Agricultural University , , ,","institution_ids":["https://openalex.org/I37574244"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037336737","display_name":"Xu Song","orcid":"https://orcid.org/0000-0003-4102-0836","observed_orcids":["https://orcid.org/0000-0003-4102-0836"]},"institutions":[{"id":"https://openalex.org/I37574244","display_name":"Sichuan Agricultural University","ror":"https://ror.org/0388c3403","country_code":"CN","type":"education","lineage":["https://openalex.org/I37574244"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Xu Song","raw_affiliation_strings":["Sichuan Agricultural University , , ,"],"raw_orcid":"https://orcid.org/0000-0003-4102-0836","affiliations":[{"raw_affiliation_string":"Sichuan Agricultural University , , ,","institution_ids":["https://openalex.org/I37574244"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086588044","display_name":"Xinghong Zhao","orcid":"https://orcid.org/0000-0003-2366-3360","observed_orcids":["https://orcid.org/0000-0003-2366-3360"]},"institutions":[{"id":"https://openalex.org/I37574244","display_name":"Sichuan Agricultural University","ror":"https://ror.org/0388c3403","country_code":"CN","type":"education","lineage":["https://openalex.org/I37574244"]},{"id":"https://openalex.org/I52158045","display_name":"China Agricultural University","ror":"https://ror.org/04v3ywz14","country_code":"CN","type":"education","lineage":["https://openalex.org/I52158045"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Xinghong Zhao","raw_affiliation_strings":["China Agricultural University , , ,","Sichuan Agricultural University , , ,"],"raw_orcid":"https://orcid.org/0000-0003-2366-3360","affiliations":[{"raw_affiliation_string":"China Agricultural University , , ,","institution_ids":["https://openalex.org/I52158045"]},{"raw_affiliation_string":"Sichuan Agricultural University , , ,","institution_ids":["https://openalex.org/I37574244"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5037336737","https://openalex.org/A5086588044"],"corresponding_institution_ids":["https://openalex.org/I37574244","https://openalex.org/I52158045"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.8051999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.8051999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.15379999577999115,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11153","display_name":"Streptococcal Infections and Treatments","score":0.00570000009611249,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.8179000020027161},{"id":"https://openalex.org/C2781253189","wikidata":"https://www.wikidata.org/wiki/Q221179","display_name":"Streptococcus pneumoniae","level":3,"score":0.7551000118255615},{"id":"https://openalex.org/C2776809874","wikidata":"https://www.wikidata.org/wiki/Q4826378","display_name":"Autolysin","level":4,"score":0.7401999831199646},{"id":"https://openalex.org/C2778253522","wikidata":"https://www.wikidata.org/wiki/Q899834","display_name":"Teichoic acid","level":4,"score":0.5806999802589417},{"id":"https://openalex.org/C2779343474","wikidata":"https://www.wikidata.org/wiki/Q3109175","display_name":"Context (archaeology)","level":2,"score":0.5076000094413757},{"id":"https://openalex.org/C2778276568","wikidata":"https://www.wikidata.org/wiki/Q190161","display_name":"Streptococcus","level":3,"score":0.4797999858856201},{"id":"https://openalex.org/C2779489039","wikidata":"https://www.wikidata.org/wiki/Q188121","display_name":"Staphylococcus aureus","level":3,"score":0.4632999897003174},{"id":"https://openalex.org/C4937899","wikidata":"https://www.wikidata.org/wiki/Q68541106","display_name":"Antimicrobial","level":2,"score":0.4544000029563904},{"id":"https://openalex.org/C2777914695","wikidata":"https://www.wikidata.org/wiki/Q12192","display_name":"Pneumonia","level":2,"score":0.4456999897956848},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4422999918460846},{"id":"https://openalex.org/C2775976552","wikidata":"https://www.wikidata.org/wiki/Q287694","display_name":"Streptococcus suis","level":4,"score":0.4359000027179718},{"id":"https://openalex.org/C2779306812","wikidata":"https://www.wikidata.org/wiki/Q131271","display_name":"Streptococcus pyogenes","level":4,"score":0.37310001254081726},{"id":"https://openalex.org/C2776925733","wikidata":"https://www.wikidata.org/wiki/Q1141979","display_name":"Haemophilus influenzae","level":3,"score":0.3652999997138977},{"id":"https://openalex.org/C2776152631","wikidata":"https://www.wikidata.org/wiki/Q4366062","display_name":"Pneumococcal infections","level":4,"score":0.3474999964237213},{"id":"https://openalex.org/C2781381541","wikidata":"https://www.wikidata.org/wiki/Q1856358","display_name":"Streptococcus oralis","level":4,"score":0.3386000096797943},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.33469998836517334},{"id":"https://openalex.org/C2776402342","wikidata":"https://www.wikidata.org/wiki/Q311275","display_name":"Haemophilus","level":3,"score":0.30250000953674316},{"id":"https://openalex.org/C2779288629","wikidata":"https://www.wikidata.org/wiki/Q199695","display_name":"Staphylococcus","level":4,"score":0.29829999804496765},{"id":"https://openalex.org/C2778663903","wikidata":"https://www.wikidata.org/wiki/Q140018","display_name":"Streptococcaceae","level":3,"score":0.28189998865127563},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.26829999685287476},{"id":"https://openalex.org/C2775897432","wikidata":"https://www.wikidata.org/wiki/Q751405","display_name":"Viridans streptococci","level":4,"score":0.2605000138282776},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.25940001010894775},{"id":"https://openalex.org/C2776993315","wikidata":"https://www.wikidata.org/wiki/Q418187","display_name":"Streptolysin","level":4,"score":0.2583000063896179}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1021/acsinfecdis.6c00944","doi":"https://doi.org/10.1021/acsinfecdis.6c00944","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acsinfecdis.6c00944","pdf_url":null,"source":{"id":"https://openalex.org/S2764646616","display_name":"ACS Infectious Diseases","issn_l":"2373-8227","issn":["2373-8227"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Infectious Diseases","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W1535577393","https://openalex.org/W1967813923","https://openalex.org/W1986270104","https://openalex.org/W1990872364","https://openalex.org/W2020779465","https://openalex.org/W2049940196","https://openalex.org/W2074029700","https://openalex.org/W2086043448","https://openalex.org/W2148328424","https://openalex.org/W2168613507","https://openalex.org/W2482873633","https://openalex.org/W2808668519","https://openalex.org/W2888904263","https://openalex.org/W2954793482","https://openalex.org/W2971827182","https://openalex.org/W3044237584","https://openalex.org/W3089124506","https://openalex.org/W3173791365","https://openalex.org/W3197946839","https://openalex.org/W3204487916","https://openalex.org/W4246863982","https://openalex.org/W4289688316","https://openalex.org/W4296585361","https://openalex.org/W4399848190","https://openalex.org/W4402557213","https://openalex.org/W4404726993","https://openalex.org/W4406463091","https://openalex.org/W4413275496","https://openalex.org/W4414817248","https://openalex.org/W4416829147"],"related_works":[],"abstract_inverted_index":{"Abstract":[0],"Rapid":[1],"detection":[2,141],"of":[3,70,93],"choline-containing":[4,76,118],"streptococcal":[5,71,119],"pathogens":[6,55,120],"is":[7,112],"important":[8],"for":[9,117,129],"timely":[10],"diagnosis":[11],"and":[12,42,121,139],"antimicrobial":[13],"treatment.":[14],"Here,":[15],"we":[16],"developed":[17],"gCBMLytA,":[18],"a":[19,30,113,125],"GFP-fused":[20],"choline-binding":[21],"module":[22,128],"derived":[23],"from":[24],"the":[25,67,91],"pneumococcal":[26],"autolysin":[27],"LytA,":[28],"as":[29,124],"fluorescence-based":[31,115],"biosensor.":[32],"gCBMLytA":[33,63,87,111],"specifically":[34],"labeled":[35],"all":[36],"14":[37],"tested":[38],"Streptococcus":[39,45],"pneumoniae":[40],"strains":[41],"also":[43],"recognized":[44],"oralis,":[46],"while":[47],"showing":[48],"no":[49],"detectable":[50],"binding":[51],"to":[52],"nontarget":[53],"ESKAPE":[54],"or":[56,102],"Haemophilus":[57],"influenzae.":[58],"These":[59],"results":[60],"indicate":[61],"that":[62,110],"recognizes":[64],"choline":[65],"within":[66],"structural":[68],"context":[69],"teichoic":[72],"acids":[73],"rather":[74],"than":[75],"structures":[77],"in":[78,90,99],"unrelated":[79],"bacteria.":[80],"In":[81],"mouse":[82],"pneumonia":[83],"models,":[84],"intravenously":[85],"administered":[86],"selectively":[88],"accumulated":[89],"lungs":[92],"S.":[94],"pneumoniae-infected":[95],"mice,":[96],"but":[97],"not":[98],"healthy":[100],"controls":[101],"Staphylococcus":[103],"aureus-infected":[104],"mice.":[105],"Together,":[106],"these":[107],"findings":[108],"demonstrate":[109],"selective":[114],"probe":[116],"may":[122],"serve":[123],"versatile":[126],"recognition":[127],"diagnostic":[130],"platforms,":[131],"including":[132],"fluorescence":[133],"imaging,":[134],"lateral":[135],"flow":[136],"assays,":[137],"ELISA,":[138],"flow-cytometry-based":[140],"systems.":[142]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2026-11-12T00:00:00"}