{"id":"https://openalex.org/W7225793345","doi":"https://doi.org/10.64898/2026.11.10.765656","title":"Hepadnavirus tropism determined by diversified NTCP functionality in Feline species","display_name":"Hepadnavirus tropism determined by diversified NTCP functionality in Feline species","publication_year":2026,"publication_date":"2026-11-11","ids":{"openalex":"https://openalex.org/W7225793345","doi":"https://doi.org/10.64898/2026.11.10.765656"},"language":"en","primary_location":{"id":"doi:10.64898/2026.11.10.765656","doi":"https://doi.org/10.64898/2026.11.10.765656","is_oa":false,"landing_page_url":"https://doi.org/10.64898/2026.11.10.765656","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016984882","display_name":"Junki Mifune","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405286871","display_name":"Japan Institute for Health Security","ror":"https://ror.org/03c8bq125","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1333985979","https://openalex.org/I4405286871"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Junki Mifune","raw_affiliation_strings":["Japan Institute for Health Security;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Japan Institute for Health Security;","institution_ids":["https://openalex.org/I4405286871"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067499258","display_name":"Toru Ekimoto","orcid":"https://orcid.org/0000-0001-7186-0292","observed_orcids":["https://orcid.org/0000-0001-7186-0292"]},"institutions":[{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Toru Ekimoto","raw_affiliation_strings":["Yokohama City University;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yokohama City University;","institution_ids":["https://openalex.org/I89630735"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006409532","display_name":"Junko S. Takeuchi","orcid":"https://orcid.org/0000-0002-4844-9013","observed_orcids":["https://orcid.org/0000-0002-4844-9013"]},"institutions":[{"id":"https://openalex.org/I4405286871","display_name":"Japan Institute for Health Security","ror":"https://ror.org/03c8bq125","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1333985979","https://openalex.org/I4405286871"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Junko S Takeuchi","raw_affiliation_strings":["Japan Institute for Health Security;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Japan Institute for Health Security;","institution_ids":["https://openalex.org/I4405286871"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062453594","display_name":"Chisa Kobayashi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405286871","display_name":"Japan Institute for Health Security","ror":"https://ror.org/03c8bq125","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1333985979","https://openalex.org/I4405286871"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Chisa Kobayashi","raw_affiliation_strings":["Japan Institute for Health Security;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Japan Institute for Health Security;","institution_ids":["https://openalex.org/I4405286871"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5105473215","display_name":"Misaki Kurata","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405286871","display_name":"Japan Institute for Health Security","ror":"https://ror.org/03c8bq125","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1333985979","https://openalex.org/I4405286871"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Misaki Kurata","raw_affiliation_strings":["Japan Institute for Health Security;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Japan Institute for Health Security;","institution_ids":["https://openalex.org/I4405286871"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061684783","display_name":"Kaho Shionoya","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405286871","display_name":"Japan Institute for Health Security","ror":"https://ror.org/03c8bq125","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1333985979","https://openalex.org/I4405286871"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kaho Shionoya","raw_affiliation_strings":["Japan Institute for Health Security;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Japan Institute for Health Security;","institution_ids":["https://openalex.org/I4405286871"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5135422203","display_name":"Miki X Kawano","orcid":"https://orcid.org/0009-0001-3495-8669","observed_orcids":["https://orcid.org/0009-0001-3495-8669"]},"institutions":[{"id":"https://openalex.org/I118574687","display_name":"University of Miyazaki","ror":"https://ror.org/0447kww10","country_code":"JP","type":"education","lineage":["https://openalex.org/I118574687"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Miki X Kawano","raw_affiliation_strings":["University of Miyazaki;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Miyazaki;","institution_ids":["https://openalex.org/I118574687"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027827712","display_name":"Akatsuki Saito","orcid":"https://orcid.org/0000-0002-2792-4399","observed_orcids":["https://orcid.org/0000-0002-2792-4399"]},"institutions":[{"id":"https://openalex.org/I118574687","display_name":"University of Miyazaki","ror":"https://ror.org/0447kww10","country_code":"JP","type":"education","lineage":["https://openalex.org/I118574687"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Akatsuki Saito","raw_affiliation_strings":["University of Miyazaki;"],"raw_orcid":"https://orcid.org/0000-0002-2792-4399","affiliations":[{"raw_affiliation_string":"University of Miyazaki;","institution_ids":["https://openalex.org/I118574687"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056297092","display_name":"Camille Sureau","orcid":"https://orcid.org/0000-0002-0517-4646","observed_orcids":["https://orcid.org/0000-0002-0517-4646"]},"institutions":[{"id":"https://openalex.org/I110017253","display_name":"Universit\u00e9 de Tours","ror":"https://ror.org/02wwzvj46","country_code":"FR","type":"education","lineage":["https://openalex.org/I110017253"]},{"id":"https://openalex.org/I154526488","display_name":"Inserm","ror":"https://ror.org/02vjkv261","country_code":"FR","type":"government","lineage":["https://openalex.org/I154526488"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Camille Sureau","raw_affiliation_strings":["INSERM U1259, Universite de Tours"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"INSERM U1259, Universite de Tours","institution_ids":["https://openalex.org/I110017253","https://openalex.org/I154526488"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069786759","display_name":"Mitsunori Ikeguchi","orcid":"https://orcid.org/0000-0003-3199-6931","observed_orcids":["https://orcid.org/0000-0003-3199-6931"]},"institutions":[{"id":"https://openalex.org/I89630735","display_name":"Yokohama City University","ror":"https://ror.org/0135d1r83","country_code":"JP","type":"education","lineage":["https://openalex.org/I89630735"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Mitsunori Ikeguchi","raw_affiliation_strings":["Yokohama City University;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yokohama City University;","institution_ids":["https://openalex.org/I89630735"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5131840977","display_name":"Koichi Watashi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405286871","display_name":"Japan Institute for Health Security","ror":"https://ror.org/03c8bq125","country_code":"JP","type":"facility","lineage":["https://openalex.org/I1333985979","https://openalex.org/I4405286871"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Koichi Watashi","raw_affiliation_strings":["Japan Institute for Health Security;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Japan Institute for Health Security;","institution_ids":["https://openalex.org/I4405286871"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10340","display_name":"Hepatitis B Virus Studies","score":0.9814000129699707,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10340","display_name":"Hepatitis B Virus Studies","score":0.9814000129699707,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.015399999916553497,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.6575999855995178},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6470999717712402},{"id":"https://openalex.org/C149011108","wikidata":"https://www.wikidata.org/wiki/Q652985","display_name":"Transporter","level":3,"score":0.5837000012397766},{"id":"https://openalex.org/C35693153","wikidata":"https://www.wikidata.org/wiki/Q239762","display_name":"Tropism","level":3,"score":0.5627999901771545},{"id":"https://openalex.org/C118892022","wikidata":"https://www.wikidata.org/wiki/Q7834587","display_name":"Transmembrane domain","level":3,"score":0.5543000102043152},{"id":"https://openalex.org/C24530287","wikidata":"https://www.wikidata.org/wiki/Q424204","display_name":"Transmembrane protein","level":3,"score":0.47510001063346863},{"id":"https://openalex.org/C33602714","wikidata":"https://www.wikidata.org/wiki/Q7809666","display_name":"Tissue tropism","level":4,"score":0.46299999952316284},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.4424000084400177},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.4422999918460846},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.4034999907016754},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.38449999690055847},{"id":"https://openalex.org/C140704245","wikidata":"https://www.wikidata.org/wiki/Q3933202","display_name":"Viral replication","level":3,"score":0.3774000108242035},{"id":"https://openalex.org/C58475186","wikidata":"https://www.wikidata.org/wiki/Q4118894","display_name":"Viral entry","level":4,"score":0.36469998955726624},{"id":"https://openalex.org/C142462285","wikidata":"https://www.wikidata.org/wiki/Q2528140","display_name":"Viral load","level":3,"score":0.35409998893737793},{"id":"https://openalex.org/C2780593183","wikidata":"https://www.wikidata.org/wiki/Q6844","display_name":"Hepatitis B virus","level":3,"score":0.35260000824928284},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.34060001373291016},{"id":"https://openalex.org/C2779904517","wikidata":"https://www.wikidata.org/wiki/Q5008900","display_name":"CATS","level":2,"score":0.3296999931335449},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.32330000400543213},{"id":"https://openalex.org/C126831891","wikidata":"https://www.wikidata.org/wiki/Q221673","display_name":"Host (biology)","level":2,"score":0.3199999928474426},{"id":"https://openalex.org/C8769409","wikidata":"https://www.wikidata.org/wiki/Q2066554","display_name":"Viral evolution","level":4,"score":0.3167000114917755},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3151000142097473},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.30649998784065247},{"id":"https://openalex.org/C2779399885","wikidata":"https://www.wikidata.org/wiki/Q413288","display_name":"Bile acid","level":2,"score":0.29760000109672546},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.29589998722076416},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.29190000891685486},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.29120001196861267},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.27970001101493835},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.2745000123977661},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.2621999979019165},{"id":"https://openalex.org/C2993683473","wikidata":"https://www.wikidata.org/wiki/Q30693176","display_name":"Amino acid substitution","level":4,"score":0.25870001316070557}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.11.10.765656","doi":"https://doi.org/10.64898/2026.11.10.765656","is_oa":false,"landing_page_url":"https://doi.org/10.64898/2026.11.10.765656","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Hepatitis":[0],"B":[1],"virus":[2],"(HBV)":[3],"exhibits":[4],"a":[5,17,154],"narrow":[6],"host":[7,172],"range,":[8],"largely":[9],"determined":[10,171],"by":[11,105],"sodium":[12],"taurocholate":[13],"cotransporting":[14],"polypeptides":[15],"(NTCP),":[16],"hepatic":[18],"bile":[19,93,161],"acid":[20,94,162],"transporter":[21,46,200],"acting":[22],"as":[23,122],"the":[24,52,159,196],"HBV":[25,79],"receptor.":[26],"NTCP":[27,53,184],"among":[28],"animals":[29],"appears":[30],"to":[31,37,100,174,192],"have":[32,101],"evolved":[33],"following":[34],"trade-off":[35],"mutations":[36],"lose":[38],"its":[39,45],"viral":[40,75,102],"receptor":[41,103,142],"activity":[42],"while":[43],"preserving":[44],"function.":[47,143,201],"Here,":[48],"through":[49],"profiling":[50],"of":[51,64,198],"homologs":[54],"from":[55,69],"23":[56],"mammalian":[57],"species,":[58],"we":[59],"uncover":[60],"unexpected":[61],"functional":[62],"heterogeneity":[63],"feline":[65],"NTCPs.":[66],"NTCPs":[67],"derived":[68],"cat,":[70],"tiger,":[71],"and":[72,78,120,131,164],"leopard":[73],"supported":[74],"preS1":[76,86,134,166],"binding":[77,87],"infection,":[80],"whereas":[81,136],"lion-NTCP":[82,140,149],"was":[83,97],"deficient":[84],"for":[85],"and,":[88],"notably,":[89],"exhibited":[90],"markedly":[91],"reduced":[92],"transport.":[95],"Cat-NTCP":[96],"further":[98],"demonstrated":[99],"function":[104],"productive":[106],"infection":[107,194],"in":[108,139,152],"primary":[109],"cat":[110,178],"hepatocytes.":[111],"Experimental":[112],"mutagenesis":[113],"identified":[114],"double":[115],"substitution":[116],"at":[117,195],"positions":[118],"191":[119],"268":[121],"key":[123],"determinants:":[124],"introducing":[125],"lion-type":[126],"substitutions":[127,138],"into":[128],"cat-NTCP":[129],"(G191R":[130],"T268A)":[132],"abolished":[133],"binding,":[135],"reciprocal":[137],"restored":[141],"Molecular":[144],"dynamics":[145],"simulations":[146],"suggested":[147],"that":[148,189],"adopts":[150],"conformation":[151],"which":[153],"transmembrane":[155],"helix":[156],"bending":[157],"enlarges":[158],"whole":[160],"tunnel":[163],"disrupts":[165],"compatibility.":[167],"Such":[168],"differences":[169],"also":[170],"susceptibility":[173],"another":[175],"feline-infected":[176],"domestic":[177],"HBV.":[179],"These":[180],"findings":[181],"reveal":[182],"diversified":[183],"functionality":[185],"with":[186],"evolutionary":[187],"plasticity":[188],"acquires":[190],"resistance":[191],"hepadnavirus":[193],"expense":[197],"physiological":[199]},"counts_by_year":[],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2026-11-12T00:00:00"}