{"id":"https://openalex.org/W7225761534","doi":"https://doi.org/10.64898/2026.11.08.765148","title":"Mechanical Fidelity of Fixed-Cell Traction Force Microscopy","display_name":"Mechanical Fidelity of Fixed-Cell Traction Force Microscopy","publication_year":2026,"publication_date":"2026-11-10","ids":{"openalex":"https://openalex.org/W7225761534","doi":"https://doi.org/10.64898/2026.11.08.765148"},"language":"en","primary_location":{"id":"doi:10.64898/2026.11.08.765148","doi":"https://doi.org/10.64898/2026.11.08.765148","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.08.765148","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.11.08.765148","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5095691199","display_name":"Armina Mortazavi","orcid":"https://orcid.org/0009-0002-2237-6159","observed_orcids":["https://orcid.org/0009-0002-2237-6159"]},"institutions":[{"id":"https://openalex.org/I200332995","display_name":"TU Dortmund University","ror":"https://ror.org/01k97gp34","country_code":"DE","type":"education","lineage":["https://openalex.org/I200332995"]},{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Armina Mortazavi","raw_affiliation_strings":["TU Dortmund and LMU Munich"],"raw_orcid":"https://orcid.org/0009-0002-2237-6159","affiliations":[{"raw_affiliation_string":"TU Dortmund and LMU Munich","institution_ids":["https://openalex.org/I200332995","https://openalex.org/I8204097"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037231935","display_name":"Jianfei Jiang","orcid":"https://orcid.org/0000-0001-9582-8705","observed_orcids":["https://orcid.org/0000-0001-9582-8705"]},"institutions":[{"id":"https://openalex.org/I200332995","display_name":"TU Dortmund University","ror":"https://ror.org/01k97gp34","country_code":"DE","type":"education","lineage":["https://openalex.org/I200332995"]},{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Jianfei Jiang","raw_affiliation_strings":["TU Dortmund and LMU Munich"],"raw_orcid":"https://orcid.org/0000-0001-9582-8705","affiliations":[{"raw_affiliation_string":"TU Dortmund and LMU Munich","institution_ids":["https://openalex.org/I200332995","https://openalex.org/I8204097"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015521956","display_name":"Benedikt Sabass","orcid":"https://orcid.org/0000-0002-7769-8043","observed_orcids":["https://orcid.org/0000-0002-7769-8043"]},"institutions":[{"id":"https://openalex.org/I200332995","display_name":"TU Dortmund University","ror":"https://ror.org/01k97gp34","country_code":"DE","type":"education","lineage":["https://openalex.org/I200332995"]},{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Benedikt Sabass","raw_affiliation_strings":["TU Dortmund and LMU Munich"],"raw_orcid":"https://orcid.org/0000-0002-7769-8043","affiliations":[{"raw_affiliation_string":"TU Dortmund and LMU Munich","institution_ids":["https://openalex.org/I200332995","https://openalex.org/I8204097"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10923","display_name":"Force Microscopy Techniques and Applications","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C38834483","wikidata":"https://www.wikidata.org/wiki/Q17000223","display_name":"Traction (geology)","level":2,"score":0.7699000239372253},{"id":"https://openalex.org/C146249460","wikidata":"https://www.wikidata.org/wiki/Q2914991","display_name":"Fixation (population genetics)","level":3,"score":0.6132000088691711},{"id":"https://openalex.org/C32520587","wikidata":"https://www.wikidata.org/wiki/Q2142522","display_name":"Tractive force","level":2,"score":0.5419999957084656},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.5271000266075134},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.4880000054836273},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.4162999987602234},{"id":"https://openalex.org/C170700871","wikidata":"https://www.wikidata.org/wiki/Q193378","display_name":"Biomechanics","level":2,"score":0.4131999909877777},{"id":"https://openalex.org/C102951782","wikidata":"https://www.wikidata.org/wiki/Q49295","display_name":"Atomic force microscopy","level":2,"score":0.3303999900817871},{"id":"https://openalex.org/C107551265","wikidata":"https://www.wikidata.org/wiki/Q1458245","display_name":"Displacement (psychology)","level":2,"score":0.30880001187324524},{"id":"https://openalex.org/C2909055331","wikidata":"https://www.wikidata.org/wiki/Q49295","display_name":"Scanning Force Microscopy","level":3,"score":0.3010999858379364},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.28139999508857727},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.274399995803833},{"id":"https://openalex.org/C67649825","wikidata":"https://www.wikidata.org/wiki/Q196538","display_name":"Microscope","level":2,"score":0.25450000166893005}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.11.08.765148","doi":"https://doi.org/10.64898/2026.11.08.765148","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.08.765148","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.11.08.765148","doi":"https://doi.org/10.64898/2026.11.08.765148","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.08.765148","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Cellular":[0],"traction":[1,77,108],"forces":[2,23],"contribute":[3],"to":[4,20,103,146],"biological":[5],"processes":[6],"including":[7],"cell":[8],"migration":[9],"and":[10,54,61,70,75,82,107,110,150],"tissue":[11],"morphogenesis.":[12],"Traction":[13],"force":[14],"microscopy":[15],"(TFM)":[16],"is":[17],"widely":[18],"used":[19],"quantify":[21],"these":[22,119],"in":[24,118,162],"living":[25],"cells,":[26],"but":[27],"it":[28],"remains":[29],"unclear":[30],"how":[31],"well":[32],"measurements":[33,144],"after":[34,55],"fixation":[35,56,59,136],"reproduce":[36],"the":[37,50,67,89,95,138,147],"live-cell":[38,99,148],"state.":[39],"Here,":[40],"we":[41],"systematically":[42],"evaluated":[43],"fixation-induced":[44],"changes":[45],"by":[46],"paired":[47],"comparison":[48],"of":[49,72,105],"same":[51],"cells":[52],"before":[53],"using":[57],"different":[58],"protocols":[60,90,114],"substrate":[62,73],"stiffnesses.":[63],"Fixation":[64],"largely":[65,122],"preserved":[66],"spatial":[68,125],"organization":[69],"direction":[71],"displacements":[74],"reconstructed":[76],"fields,":[78],"whereas":[79],"displacement,":[80],"traction,":[81],"strain-energy":[83],"magnitudes":[84,109],"showed":[85],"fixation-dependent":[86],"changes.":[87],"Among":[88],"tested,":[91],"glutaraldehyde":[92],"(GA)":[93],"provided":[94],"closest":[96],"agreement":[97],"with":[98,134,158],"measurements,":[100],"retaining":[101],"up":[102],"99%":[104],"displacement":[106],"strain":[111],"energy.":[112],"Paraformaldehyde-based":[113],"caused":[115],"larger":[116],"reductions":[117],"quantities":[120],"while":[121],"preserving":[123],"their":[124],"organization.":[126],"These":[127],"results":[128],"show":[129],"that":[130],"fixed-cell":[131],"TFM,":[132],"particularly":[133],"GA":[135],"under":[137],"conditions":[139],"tested":[140],"here,":[141],"can":[142],"provide":[143],"close":[145],"state":[149],"offers":[151],"a":[152,163],"practical":[153],"approach":[154],"for":[155],"combining":[156],"TFM":[157],"post-fixation":[159],"fluorescence":[160],"imaging":[161],"multi-well":[164],"workflow.":[165]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-11T00:00:00"}