{"id":"https://openalex.org/W7225756463","doi":"https://doi.org/10.1038/s41598-026-79130-9","title":"Transglutaminase 1 is conserved in amphibians and required for membrane-associated transglutaminase activity in the epidermis of clawed frogs","display_name":"Transglutaminase 1 is conserved in amphibians and required for membrane-associated transglutaminase activity in the epidermis of clawed frogs","publication_year":2026,"publication_date":"2026-11-10","ids":{"openalex":"https://openalex.org/W7225756463","doi":"https://doi.org/10.1038/s41598-026-79130-9"},"language":"en","primary_location":{"id":"doi:10.1038/s41598-026-79130-9","doi":"https://doi.org/10.1038/s41598-026-79130-9","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-026-79130-9","pdf_url":null,"source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1038/s41598-026-79130-9","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5018182272","display_name":"Attila Placido Sachslehner","orcid":"https://orcid.org/0000-0003-1447-9254","observed_orcids":["https://orcid.org/0000-0003-1447-9254"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Attila Placido Sachslehner","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-1447-9254","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5130933919","display_name":"Arne Christiaens","orcid":"https://orcid.org/0000-0002-3116-9920","observed_orcids":["https://orcid.org/0000-0002-3116-9920"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Arne Christiaens","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-3116-9920","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035756045","display_name":"Marjolein Carron","orcid":"https://orcid.org/0000-0002-5121-2711","observed_orcids":["https://orcid.org/0000-0002-5121-2711"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Marjolein Carron","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-5121-2711","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5093688535","display_name":"Julia Steinbinder","orcid":"https://orcid.org/0009-0006-4208-9455","observed_orcids":["https://orcid.org/0009-0006-4208-9455"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Julia Steinbinder","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0009-0006-4208-9455","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015545713","display_name":"Bahar Golabi","orcid":"https://orcid.org/0000-0002-0974-8280","observed_orcids":["https://orcid.org/0000-0002-0974-8280"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bahar Golabi","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-0974-8280","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066624145","display_name":"Kris Vleminckx","orcid":"https://orcid.org/0000-0002-9666-4105","observed_orcids":["https://orcid.org/0000-0002-9666-4105"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kris Vleminckx","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-9666-4105","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5000405419","display_name":"Leopold Eckhart","orcid":"https://orcid.org/0000-0002-5645-2036","observed_orcids":["https://orcid.org/0000-0002-5645-2036"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Leopold Eckhart","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-5645-2036","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":2690,"currency":"USD","value_usd":2690},"apc_paid":{"value":2690,"currency":"USD","value_usd":2690},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11650","display_name":"Skin and Cellular Biology Research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11650","display_name":"Skin and Cellular Biology Research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12355","display_name":"Genetic and rare skin diseases.","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2708","display_name":"Dermatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7548999786376953},{"id":"https://openalex.org/C178498320","wikidata":"https://www.wikidata.org/wiki/Q14903608","display_name":"Tissue transglutaminase","level":3,"score":0.6782000064849854},{"id":"https://openalex.org/C2779535977","wikidata":"https://www.wikidata.org/wiki/Q1342298","display_name":"Xenopus","level":3,"score":0.6316999793052673},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6198999881744385},{"id":"https://openalex.org/C2776458125","wikidata":"https://www.wikidata.org/wiki/Q1827343","display_name":"Epidermis (zoology)","level":2,"score":0.5449000000953674},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.49459999799728394},{"id":"https://openalex.org/C2781465898","wikidata":"https://www.wikidata.org/wiki/Q10908","display_name":"Amphibian","level":2,"score":0.4381999969482422},{"id":"https://openalex.org/C199216141","wikidata":"https://www.wikidata.org/wiki/Q4995178","display_name":"Conserved sequence","level":4,"score":0.4318000078201294},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4287000000476837},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.3693000078201294},{"id":"https://openalex.org/C2778935311","wikidata":"https://www.wikidata.org/wiki/Q1746027","display_name":"Salientia","level":4,"score":0.35089999437332153},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.33980000019073486},{"id":"https://openalex.org/C2776879804","wikidata":"https://www.wikidata.org/wiki/Q7630919","display_name":"Subcellular localization","level":3,"score":0.3244999945163727},{"id":"https://openalex.org/C12125453","wikidata":"https://www.wikidata.org/wiki/Q5156837","display_name":"Compound heterozygosity","level":4,"score":0.2971999943256378},{"id":"https://openalex.org/C2777948982","wikidata":"https://www.wikidata.org/wiki/Q584203","display_name":"Pleurodeles","level":3,"score":0.2879999876022339},{"id":"https://openalex.org/C2777103240","wikidata":"https://www.wikidata.org/wiki/Q18356482","display_name":"Frog Skin","level":3,"score":0.2854999899864197},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.27799999713897705},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.26759999990463257},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.262800008058548},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.25859999656677246}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1038/s41598-026-79130-9","doi":"https://doi.org/10.1038/s41598-026-79130-9","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-026-79130-9","pdf_url":null,"source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1038/s41598-026-79130-9","doi":"https://doi.org/10.1038/s41598-026-79130-9","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-026-79130-9","pdf_url":null,"source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Abstract":[0],"Transglutaminase":[1],"1":[2],"(TGM1)":[3],"plays":[4],"a":[5,61,69,196],"central":[6],"role":[7,47,181],"in":[8,19,39,52,74,91,100,112,129,184,195],"the":[9,14,17,33,40,46,53,75,113,124,130,189],"cross-linking":[10],"of":[11,16,22,35,48,79,110,115,121,132,144,155,173,179,182,199],"proteins":[12],"during":[13,170],"cornification":[15,172,193],"epidermis":[18,131],"mammals.":[20],"Mutations":[21],"human":[23],"TGM1":[24,49],"lead":[25],"to":[26,138],"TGM1-nonsyndromic":[27],"epidermal":[28,171],"differentiation":[29],"disorder":[30],"(TGM1-nEDD),":[31],"and":[32,84,94,141,186,201],"deletion":[34],"Tgm1":[36,102,162,183],"is":[37,50,72,105,163],"lethal":[38],"mouse.":[41],"Here,":[42],"we":[43],"investigated":[44],"whether":[45],"conserved":[51,73,99],"western":[54],"clawed":[55],"frog":[56],"(":[57],"Xenopus":[58],"tropicalis":[59,117,134,175],"),":[60],"model":[62],"species":[63],"for":[64],"amphibians.":[65],"We":[66],"found":[67],"that":[68,191],"tgm1":[70,104,122,145,156],"gene":[71],"three":[76],"main":[77],"clades":[78],"amphibians,":[80],"namely":[81],"anurans,":[82],"caudates":[83],"caecilians.":[85],"The":[86,177],"amino":[87],"acid":[88],"residues":[89],"implicated":[90],"catalytic":[92],"activity":[93,128],"palmitoylation-dependent":[95],"membrane":[96],"anchorage":[97],"are":[98],"amphibian":[101],"proteins.":[103],"expressed":[106],"besides":[107],"multiple":[108],"homologs":[109],"tgm3":[111],"skin":[114],"X.":[116,133,174],".":[118,135,176],"CRISPR-Cas9-mediated":[119],"disruption":[120],"abolished":[123],"cell":[125,168],"membrane-associated":[126],"TGM":[127],"In":[136,160],"contrast":[137],"mosaic":[139],"mutants":[140,143],"heterozygous":[142,148],",":[146],"compound":[147],"frogs":[149,185],"carrying":[150],"two":[151],"predicted":[152],"loss-of-function":[153],"alleles":[154],"died":[157],"around":[158],"metamorphosis.":[159],"conclusion,":[161],"catalytically":[164],"active":[165],"at":[166],"keratinocyte":[167],"membranes":[169],"conservation":[178],"this":[180],"mammals":[187],"supports":[188],"hypothesis":[190],"Tgm1-dependent":[192],"evolved":[194],"common":[197],"ancestor":[198],"amphibians":[200],"amniotes.":[202]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-11T00:00:00"}