{"id":"https://openalex.org/W7225743392","doi":"https://doi.org/10.1186/s12885-026-17341-z","title":"Phosphatidylserine modulation influences syncytia formation in human breast cancer cell lines","display_name":"Phosphatidylserine modulation influences syncytia formation in human breast cancer cell lines","publication_year":2026,"publication_date":"2026-11-10","ids":{"openalex":"https://openalex.org/W7225743392","doi":"https://doi.org/10.1186/s12885-026-17341-z"},"language":"en","primary_location":{"id":"doi:10.1186/s12885-026-17341-z","doi":"https://doi.org/10.1186/s12885-026-17341-z","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12885-026-17341-z","pdf_url":null,"source":{"id":"https://openalex.org/S192926780","display_name":"BMC Cancer","issn_l":"1471-2407","issn":["1471-2407"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Cancer","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1186/s12885-026-17341-z","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5006214692","display_name":"Mareike Sieler","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155976169","display_name":"Witten/Herdecke University","ror":"https://ror.org/00yq55g44","country_code":"DE","type":"education","lineage":["https://openalex.org/I155976169"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Mareike Sieler","raw_affiliation_strings":["Immunology and Tumor Biology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Str. 10, 58453, Witten, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immunology and Tumor Biology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Str. 10, 58453, Witten, Germany","institution_ids":["https://openalex.org/I155976169"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161390048","display_name":"Leonie Huwa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155976169","display_name":"Witten/Herdecke University","ror":"https://ror.org/00yq55g44","country_code":"DE","type":"education","lineage":["https://openalex.org/I155976169"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Leonie Huwa","raw_affiliation_strings":["Immunology and Tumor Biology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Str. 10, 58453, Witten, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immunology and Tumor Biology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Str. 10, 58453, Witten, Germany","institution_ids":["https://openalex.org/I155976169"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048623416","display_name":"Silvia Keil","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155976169","display_name":"Witten/Herdecke University","ror":"https://ror.org/00yq55g44","country_code":"DE","type":"education","lineage":["https://openalex.org/I155976169"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Silvia Keil","raw_affiliation_strings":["Immunology and Tumor Biology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Str. 10, 58453, Witten, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immunology and Tumor Biology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Str. 10, 58453, Witten, Germany","institution_ids":["https://openalex.org/I155976169"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036720243","display_name":"Maja Kneissig","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I155976169","display_name":"Witten/Herdecke University","ror":"https://ror.org/00yq55g44","country_code":"DE","type":"education","lineage":["https://openalex.org/I155976169"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Maja Kneissig","raw_affiliation_strings":["Immunology and Tumor Biology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Str. 10, 58453, Witten, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Immunology and Tumor Biology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Str. 10, 58453, Witten, Germany","institution_ids":["https://openalex.org/I155976169"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091824396","display_name":"Thomas Dittmar","orcid":"https://orcid.org/0000-0001-8505-3424","observed_orcids":["https://orcid.org/0000-0001-8505-3424"]},"institutions":[{"id":"https://openalex.org/I155976169","display_name":"Witten/Herdecke University","ror":"https://ror.org/00yq55g44","country_code":"DE","type":"education","lineage":["https://openalex.org/I155976169"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Thomas Dittmar","raw_affiliation_strings":["Immunology and Tumor Biology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Str. 10, 58453, Witten, Germany"],"raw_orcid":"https://orcid.org/0000-0001-8505-3424","affiliations":[{"raw_affiliation_string":"Immunology and Tumor Biology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Str. 10, 58453, Witten, Germany","institution_ids":["https://openalex.org/I155976169"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5091824396"],"corresponding_institution_ids":["https://openalex.org/I155976169"],"apc_list":{"value":3390,"currency":"USD","value_usd":3390},"apc_paid":{"value":3390,"currency":"USD","value_usd":3390},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.6176999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.6176999807357788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12465","display_name":"Cancer, Lipids, and Metabolism","score":0.09380000084638596,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.04129999876022339,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C2779637612","wikidata":"https://www.wikidata.org/wiki/Q2354337","display_name":"Phosphatidylserine","level":4,"score":0.7588000297546387},{"id":"https://openalex.org/C92488253","wikidata":"https://www.wikidata.org/wiki/Q56592403","display_name":"Cell fusion","level":3,"score":0.6589999794960022},{"id":"https://openalex.org/C96232424","wikidata":"https://www.wikidata.org/wiki/Q4118072","display_name":"Cancer cell","level":3,"score":0.6500999927520752},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.5411999821662903},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.5271999835968018},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5157999992370605},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.5073000192642212},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.47929999232292175},{"id":"https://openalex.org/C88634738","wikidata":"https://www.wikidata.org/wiki/Q24769543","display_name":"Annexin","level":3,"score":0.4643999934196472},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4512999951839447},{"id":"https://openalex.org/C40479148","wikidata":"https://www.wikidata.org/wiki/Q844460","display_name":"Syncytium","level":3,"score":0.3977999985218048},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37619999051094055},{"id":"https://openalex.org/C2780670749","wikidata":"https://www.wikidata.org/wiki/Q14908239","display_name":"Annexin A5","level":4,"score":0.36239999532699585},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.3553999960422516},{"id":"https://openalex.org/C109541995","wikidata":"https://www.wikidata.org/wiki/Q1881253","display_name":"MCF-7","level":5,"score":0.35269999504089355},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.34130001068115234},{"id":"https://openalex.org/C179464577","wikidata":"https://www.wikidata.org/wiki/Q1632589","display_name":"Jurkat cells","level":4,"score":0.3402999937534332},{"id":"https://openalex.org/C188810444","wikidata":"https://www.wikidata.org/wiki/Q3476415","display_name":"Phospholipid scramblase","level":5,"score":0.3100000023841858},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.3041999936103821},{"id":"https://openalex.org/C530470458","wikidata":"https://www.wikidata.org/wiki/Q128581","display_name":"Breast cancer","level":3,"score":0.299699991941452},{"id":"https://openalex.org/C2778106830","wikidata":"https://www.wikidata.org/wiki/Q29548","display_name":"Cell membrane","level":3,"score":0.28380000591278076},{"id":"https://openalex.org/C152000582","wikidata":"https://www.wikidata.org/wiki/Q567522","display_name":"Anoikis","level":4,"score":0.27959999442100525},{"id":"https://openalex.org/C79266657","wikidata":"https://www.wikidata.org/wiki/Q424178","display_name":"Lipid raft","level":3,"score":0.2775000035762787},{"id":"https://openalex.org/C30345302","wikidata":"https://www.wikidata.org/wiki/Q14908233","display_name":"Annexin A2","level":4,"score":0.2630999982357025},{"id":"https://openalex.org/C2777877089","wikidata":"https://www.wikidata.org/wiki/Q411909","display_name":"Salinomycin","level":3,"score":0.2572999894618988},{"id":"https://openalex.org/C173414695","wikidata":"https://www.wikidata.org/wiki/Q5510276","display_name":"Fusion mechanism","level":4,"score":0.25029999017715454}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1186/s12885-026-17341-z","doi":"https://doi.org/10.1186/s12885-026-17341-z","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12885-026-17341-z","pdf_url":null,"source":{"id":"https://openalex.org/S192926780","display_name":"BMC Cancer","issn_l":"1471-2407","issn":["1471-2407"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Cancer","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1186/s12885-026-17341-z","doi":"https://doi.org/10.1186/s12885-026-17341-z","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12885-026-17341-z","pdf_url":null,"source":{"id":"https://openalex.org/S192926780","display_name":"BMC Cancer","issn_l":"1471-2407","issn":["1471-2407"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Cancer","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Abstract":[0],"Background":[1],"Cell-cell":[2],"fusion":[3,19,34,81,89,98,208,288,351,375],"is":[4,20,35,38,71,310],"a":[5],"biological":[6],"process,":[7],"crucial":[8],"for":[9],"the":[10,52,93,103,110,113,123,132,145,151,163,177,200,206,211,231,242,247,264,272,277,305,312,319,324,329,343,346,361,366,379],"development":[11],"and":[12,43,100,118,170,197,237,355],"homeostasis":[13],"of":[14,72,102,112,210,244,307,321,326,348,363],"tissue.":[15],"In":[16,180],"contrast,":[17],"cell":[18,29,33,80,88,97,126,135,207,229,273,287,315,332,339,374],"also":[21],"pathologically":[22],"associated":[23],"with":[24,48,131,193,255],"viral":[25],"infections":[26],"or":[27,65,138,174,214,250,323],"cancer":[28,45,54,79,87,134,331],"fusion.":[30,274,316,340],"While":[31,220],"physiological":[32],"strictly":[36],"regulated,":[37],"it":[39,70,282,370],"not":[40,240],"clear":[41],"how":[42,78],"why":[44],"cells":[46,56,216,294,309],"fuse":[47,254],"other":[49,256],"cells.":[50,257],"Since":[51],"resulting":[53],"hybrid":[55],"can":[57],"have":[58],"new":[59],"malignant":[60],"properties,":[61],"like":[62],"chemotherapeutic":[63],"resistance":[64],"an":[66],"enhanced":[67],"metastatic":[68],"potential,":[69],"interest":[73],"to":[74,76,108,172,225,253,336,372],"get":[75],"know":[77],"takes":[82],"place.":[83],"Methods":[84],"A":[85],"breast":[86,124,133,330],"model,":[90],"relying":[91],"on":[92,246,271,365,378],"dual":[94],"split":[95],"protein":[96,147],"assay":[99],"overexpression":[101,266,325],"fusogen":[104],"Syncytin-1,":[105],"was":[106,129,160,183,190,223,283,334],"used":[107,165],"study":[109],"impact":[111],"membrane":[114,306],"lipid":[115,119],"phosphatidylserine":[116,245],"(PS)":[117],"distributing":[120],"proteins.":[121],"Therefore,":[122],"epithelial":[125],"line":[127],"M13SV1":[128,293],"cocultured":[130],"lines":[136,333],"MDA-MB-231":[137],"HS578T.":[139],"To":[140],"mask":[141],"PS,":[142],"we":[143],"added":[144],"PS-binding":[146],"Annexin":[148,221],"V":[149,222],"in":[150,304,314,328],"coculturing":[152],"medium.":[153],"The":[154,187,258],"most":[155],"prominent":[156],"expressed":[157],"scramblase":[158],"Ano6/TMEM16F":[159,259],"investigated":[161],"using":[162,185],"clinically":[164],"substances":[166,233],"Niclosamide,":[167,234],"Clofazimine,":[168,235],"Salinomycin":[169,236],"Hexachlorophene":[171,238],"indirectly":[173],"directly":[175],"inhibit":[176],"protein\u2019s":[178],"activity.":[179],"addition,":[181],"Ano6":[182,322],"knocked-out":[184],"CRISPR/Cas9.":[186],"flippase":[188],"Atp8a1":[189],"simultaneously":[191],"overexpressed":[192],"its":[194],"cofactor":[195],"CDC50a":[196],"assays":[198],"assessing":[199],"tumorigenic":[201],"properties":[202],"as":[203,205,261,263],"well":[204,262],"capacity":[209,252],"differently":[212],"treated":[213],"altered":[215],"were":[217,279,297],"done.":[218],"Results":[219],"able":[224,335],"significantly":[226],"decrease":[227,313],"Syncytin-1-mediated":[228,286,338],"fusion,":[230],"four":[232],"did":[239],"affect":[241],"amount":[243],"cells\u2019":[248],"surface":[249],"their":[251],"KO":[260],"Atp8a1-CDC50a":[265,327],"showed":[267],"rather":[268],"non-significant":[269],"effects":[270,278],"Conclusion":[275],"Although":[276],"mainly":[280],"non-significant,":[281],"shown,":[284],"that":[285,301,345,356],"could":[289],"be":[290,353],"decreased":[291],"when":[292,376],"overexpressing":[295,298],"Syncytin-1":[296,364],"Atp8a1-CDC50a,":[299],"suggesting":[300],"less":[302],"PS":[303,349,358],"these":[308],"causing":[311],"Vice":[317],"versa,":[318],"loss":[320],"increase":[337],"This":[341],"suggests":[342],"hypothesis":[344],"localization":[347],"during":[350],"may":[352,359],"dynamic":[354],"externalized":[357],"enhance":[360],"function":[362],"expressing":[367],"cell,":[368],"while":[369],"appears":[371],"disrupt":[373],"exposed":[377],"neighboring":[380],"cell.":[381]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-11T00:00:00"}