{"id":"https://openalex.org/W7225740692","doi":"https://doi.org/10.21203/rs.3.rs-11433369/v1","title":"Isolation, genomic and comparative proteomic characterization of three lytic Pseudomonas aeruginosa bacteriophages from wastewater in Bobo-Dioulasso, Burkina Faso","display_name":"Isolation, genomic and comparative proteomic characterization of three lytic Pseudomonas aeruginosa bacteriophages from wastewater in Bobo-Dioulasso, Burkina Faso","publication_year":2026,"publication_date":"2026-11-10","ids":{"openalex":"https://openalex.org/W7225740692","doi":"https://doi.org/10.21203/rs.3.rs-11433369/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-11433369/v1","doi":"https://doi.org/10.21203/rs.3.rs-11433369/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11433369/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.21203/rs.3.rs-11433369/v1","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005824014","display_name":"Kobo Gnada","orcid":"https://orcid.org/0009-0000-1687-7905","observed_orcids":["https://orcid.org/0009-0000-1687-7905"]},"institutions":[{"id":"https://openalex.org/I3131918342","display_name":"Nazi Boni University","ror":"https://ror.org/04cq90n15","country_code":"BF","type":"education","lineage":["https://openalex.org/I3131918342"]}],"countries":["BF"],"is_corresponding":false,"raw_author_name":"Kobo Gnada","raw_affiliation_strings":["Nazi Boni University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Nazi Boni University","institution_ids":["https://openalex.org/I3131918342"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161395101","display_name":"Edmond Wend-Guudi Silga","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I3131918342","display_name":"Nazi Boni University","ror":"https://ror.org/04cq90n15","country_code":"BF","type":"education","lineage":["https://openalex.org/I3131918342"]}],"countries":["BF"],"is_corresponding":false,"raw_author_name":"Edmond Wend-Guudi Silga","raw_affiliation_strings":["Nazi Boni University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Nazi Boni University","institution_ids":["https://openalex.org/I3131918342"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003704656","display_name":"Christiane Essoh","orcid":"https://orcid.org/0009-0001-7848-117X","observed_orcids":["https://orcid.org/0009-0001-7848-117X"]},"institutions":[{"id":"https://openalex.org/I4210126051","display_name":"Universit\u00e9 Pelefero Gon Coulibaly","ror":"https://ror.org/0358nsq19","country_code":"CI","type":"education","lineage":["https://openalex.org/I4210126051"]}],"countries":["CI"],"is_corresponding":false,"raw_author_name":"Christiane You Essoh","raw_affiliation_strings":["P\u00e9l\u00e9foro Gon Coulibaly University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"P\u00e9l\u00e9foro Gon Coulibaly University","institution_ids":["https://openalex.org/I4210126051"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038883743","display_name":"Raymond Karlhis Yao","orcid":"https://orcid.org/0009-0003-1941-676X","observed_orcids":["https://orcid.org/0009-0003-1941-676X"]},"institutions":[{"id":"https://openalex.org/I3131918342","display_name":"Nazi Boni University","ror":"https://ror.org/04cq90n15","country_code":"BF","type":"education","lineage":["https://openalex.org/I3131918342"]}],"countries":["BF"],"is_corresponding":false,"raw_author_name":"Raymond Karlhis Yao","raw_affiliation_strings":["Nazi Boni University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Nazi Boni University","institution_ids":["https://openalex.org/I3131918342"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161395037","display_name":"Arnaud Quetin Sanou","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I3131918342","display_name":"Nazi Boni University","ror":"https://ror.org/04cq90n15","country_code":"BF","type":"education","lineage":["https://openalex.org/I3131918342"]}],"countries":["BF"],"is_corresponding":false,"raw_author_name":"Arnaud Quetin Sanou","raw_affiliation_strings":["Nazi Boni University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Nazi Boni University","institution_ids":["https://openalex.org/I3131918342"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004463624","display_name":"A\u00efda Ouangraoua","orcid":"https://orcid.org/0000-0002-2040-4948","observed_orcids":["https://orcid.org/0000-0002-2040-4948"]},"institutions":[{"id":"https://openalex.org/I135117807","display_name":"Universit\u00e9 de Sherbrooke","ror":"https://ror.org/00kybxq39","country_code":"CA","type":"education","lineage":["https://openalex.org/I135117807"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"A\u00efda Ouangraoua","raw_affiliation_strings":["University of Sherbrooke"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Sherbrooke","institution_ids":["https://openalex.org/I135117807"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5161368315","display_name":"Michel Kir\u00e9opori Gomgnimbou","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I3131918342","display_name":"Nazi Boni University","ror":"https://ror.org/04cq90n15","country_code":"BF","type":"education","lineage":["https://openalex.org/I3131918342"]}],"countries":["BF"],"is_corresponding":false,"raw_author_name":"Michel Kir\u00e9opori Gomgnimbou","raw_affiliation_strings":["Nazi BONI University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Nazi BONI University","institution_ids":["https://openalex.org/I3131918342"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13220","display_name":"Plant and Fungal Interactions Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C182615771","wikidata":"https://www.wikidata.org/wiki/Q1369386","display_name":"Lytic cycle","level":3,"score":0.95660001039505},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.794700026512146},{"id":"https://openalex.org/C2777637488","wikidata":"https://www.wikidata.org/wiki/Q31856","display_name":"Pseudomonas aeruginosa","level":3,"score":0.727400004863739},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.7174000144004822},{"id":"https://openalex.org/C50929876","wikidata":"https://www.wikidata.org/wiki/Q164280","display_name":"Subfamily","level":3,"score":0.7131999731063843},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5830000042915344},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.5630000233650208},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.4733000099658966},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4708000123500824},{"id":"https://openalex.org/C126831891","wikidata":"https://www.wikidata.org/wiki/Q221673","display_name":"Host (biology)","level":2,"score":0.45399999618530273},{"id":"https://openalex.org/C57326107","wikidata":"https://www.wikidata.org/wiki/Q4244196","display_name":"Phage typing","level":3,"score":0.42910000681877136},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.41290000081062317},{"id":"https://openalex.org/C2780984408","wikidata":"https://www.wikidata.org/wiki/Q6770","display_name":"Myoviridae","level":5,"score":0.37959998846054077},{"id":"https://openalex.org/C2779256223","wikidata":"https://www.wikidata.org/wiki/Q611870","display_name":"Phage therapy","level":5,"score":0.3450999855995178},{"id":"https://openalex.org/C24432333","wikidata":"https://www.wikidata.org/wiki/Q2068526","display_name":"Whole genome sequencing","level":4,"score":0.3019999861717224},{"id":"https://openalex.org/C2776738503","wikidata":"https://www.wikidata.org/wiki/Q138058","display_name":"Pseudomonadaceae","level":4,"score":0.28839999437332153},{"id":"https://openalex.org/C60987743","wikidata":"https://www.wikidata.org/wiki/Q1460232","display_name":"Virulence","level":3,"score":0.27570000290870667},{"id":"https://openalex.org/C2781463711","wikidata":"https://www.wikidata.org/wiki/Q131143","display_name":"Pseudomonas","level":3,"score":0.26249998807907104}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-11433369/v1","doi":"https://doi.org/10.21203/rs.3.rs-11433369/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11433369/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-11433369/v1","doi":"https://doi.org/10.21203/rs.3.rs-11433369/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11433369/v1","pdf_url":null,"source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":65,"referenced_works":["https://openalex.org/W21889920","https://openalex.org/W1555607392","https://openalex.org/W1919681724","https://openalex.org/W1974394752","https://openalex.org/W1982939204","https://openalex.org/W1984693521","https://openalex.org/W1995510650","https://openalex.org/W2008137668","https://openalex.org/W2031611770","https://openalex.org/W2040416009","https://openalex.org/W2041257508","https://openalex.org/W2056377972","https://openalex.org/W2057891171","https://openalex.org/W2109532955","https://openalex.org/W2114853012","https://openalex.org/W2116788484","https://openalex.org/W2119497560","https://openalex.org/W2119859604","https://openalex.org/W2120902911","https://openalex.org/W2147351570","https://openalex.org/W2160378127","https://openalex.org/W2161973008","https://openalex.org/W2163970869","https://openalex.org/W2169899471","https://openalex.org/W2170551349","https://openalex.org/W2174377965","https://openalex.org/W2418055506","https://openalex.org/W2443992611","https://openalex.org/W2463894809","https://openalex.org/W2605216580","https://openalex.org/W2803245377","https://openalex.org/W2807161463","https://openalex.org/W2898120697","https://openalex.org/W2941342985","https://openalex.org/W2941437619","https://openalex.org/W2945698955","https://openalex.org/W2950044547","https://openalex.org/W2982531601","https://openalex.org/W2988396470","https://openalex.org/W2988892352","https://openalex.org/W2997259491","https://openalex.org/W3009737049","https://openalex.org/W3030304545","https://openalex.org/W3041773305","https://openalex.org/W3097407122","https://openalex.org/W3118048495","https://openalex.org/W3189053267","https://openalex.org/W3206166497","https://openalex.org/W4200286045","https://openalex.org/W4206677002","https://openalex.org/W4211094053","https://openalex.org/W4212901338","https://openalex.org/W4224122247","https://openalex.org/W4281725105","https://openalex.org/W4313560729","https://openalex.org/W4367312522","https://openalex.org/W4367599433","https://openalex.org/W4368358104","https://openalex.org/W4385749849","https://openalex.org/W4394125659","https://openalex.org/W4394785938","https://openalex.org/W4407178793","https://openalex.org/W4412708795","https://openalex.org/W4413301878","https://openalex.org/W7118953414"],"related_works":[],"abstract_inverted_index":{"Abstract":[0],"Background.":[1],"Pseudomonas":[2],"aeruginosa":[3,111,137,327],"is":[4,14,65,83,207,252,295],"a":[5,143,208,253,302,359],"leading":[6],"cause":[7],"of":[8,37,77,145,161,210,256,335,350],"opportunistic":[9],"and":[10,13,46,53,62,80,119,140,148,165,186,224,263,278,281,288,300,348,363],"healthcare-associated":[11],"infections":[12],"listed":[15],"by":[16],"the":[17,22,38,84,162,196,211,237,308,324],"World":[18],"Health":[19],"Organization":[20],"among":[21],"pathogens":[23],"for":[24,88,183,358,364],"which":[25,69],"new":[26,254,267,338],"therapeutic":[27,366],"options":[28],"are":[29,35,92,282,323],"most":[30,85],"urgently":[31],"needed.":[32],"Lytic":[33],"bacteriophages":[34],"one":[36],"few":[39],"alternatives":[40],"that":[41,91,250],"can":[42],"be":[43],"deployed":[44],"rapidly":[45],"at":[47,158],"low":[48],"cost,":[49],"but":[50],"phage":[51,54,328,361],"collections":[52],"genome":[55],"sequences":[56],"remain":[57],"very":[58],"unevenly":[59],"distributed":[60],"geographically,":[61],"West":[63],"Africa":[64],"particularly":[66],"under-represented.":[67],"Wastewater,":[68],"combines":[70],"high":[71],"bacterial":[72],"densities":[73],"with":[74,232,285,289,317],"continuous":[75],"inputs":[76],"faecal,":[78],"hospital":[79],"industrial":[81],"effluent,":[82],"productive":[86],"environment":[87],"recovering":[89],"phages":[90,106,127,180],"locally":[93],"adapted":[94],"to":[95,103,120,176,195,236,297,301],"circulating":[96],"clinical":[97,109,147],"strains.":[98,150,178],"We":[99],"therefore":[100],"set":[101],"out":[102],"isolate":[104,255],"lytic":[105,126,345],"active":[107],"against":[108,142],"P.":[110,136,326],"isolates":[112,156,173],"from":[113,130,331],"wastewater":[114,132],"in":[115],"Bobo-Dioulasso,":[116],"Burkina":[117,332],"Faso,":[118],"characterize":[121],"their":[122,290,293],"genomes.":[123],"Results.":[124],"Twenty-eight":[125],"were":[128,153,174,181],"isolated":[129],"five":[131],"sites":[133],"using":[134],"six":[135],"host":[138,342],"strains,":[139],"screened":[141],"panel":[144],"eleven":[146,155,163],"reference":[149],"Host":[151],"ranges":[152],"broad:":[154],"lysed":[157,167],"least":[159],"ten":[160],"strains":[164],"three":[166,187,193,321],"all":[168],"eleven,":[169],"while":[170],"only":[171],"two":[172,266,271],"restricted":[175],"four":[177],"Five":[179],"selected":[182],"whole-genome":[184],"sequencing":[185],"yielded":[188],"complete,":[189],"high-quality":[190],"assemblies.":[191],"All":[192],"belong":[194,235],"class":[197],"Caudoviricetes":[198],".":[199,220,246],"vB_PaeM_CM0001":[200,251,312],"(57,614":[201],"bp;":[202],"63.5%":[203],"GC;":[204],"88":[205],"CDSs)":[206],"member":[209],"family":[212,238],"Mesyanzhinovviridae":[213],",":[214,217,240,243],"subfamily":[215,241],"Bradleyvirinae":[216],"genus":[218,244],"Abidjanvirus":[219,259],"vB_PaeP_CM0002":[221,262],"(43,491":[222],"bp)":[223],"vB_PaeP_CM0003":[225,264],"(43,177":[226],"bp),":[227],"each":[228,286],"encoding":[229],"65":[230],"CDSs":[231],"62.3\u201362.6%":[233],"GC,":[234],"Autographiviridae":[239],"Krylovirinae":[242],"Phikmvvirus":[245,268,272],"Intergenomic":[247],"similarities":[248],"indicate":[249],"an":[257],"existing":[258],"species,":[260],"whereas":[261],"represent":[265,337],"species.":[269,339],"The":[270],"genomes":[273,322,329],"share":[274],"91.9%":[275],"nucleotide":[276,298],"identity":[277],"62":[279],"orthogroups":[280,316],"fully":[283],"collinear":[284],"other":[287],"closest":[291],"relatives;":[292],"divergence":[294],"confined":[296],"substitution":[299],"single":[303],"2.0":[304],"kb":[305],"region":[306],"spanning":[307],"tail":[309],"fibre":[310],"locus.":[311],"shares":[313],"no":[314],"detectable":[315],"them.":[318],"Conclusions.":[319],"These":[320],"first":[325],"reported":[330],"Faso.":[333],"Two":[334],"them":[336,354],"Their":[340],"broad":[341],"ranges,":[343],"strictly":[344],"gene":[346],"repertoires":[347],"absence":[349],"resistance":[351],"determinants":[352],"make":[353],"credible":[355],"starting":[356],"points":[357],"national":[360],"bank":[362],"future":[365],"evaluation.":[367]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-11T00:00:00"}