{"id":"https://openalex.org/W7225739299","doi":"https://doi.org/10.64898/2026.11.04.764183","title":"EngA adopts multiple distinct conformational states during ribosome assembly","display_name":"EngA adopts multiple distinct conformational states during ribosome assembly","publication_year":2026,"publication_date":"2026-11-10","ids":{"openalex":"https://openalex.org/W7225739299","doi":"https://doi.org/10.64898/2026.11.04.764183"},"language":"en","primary_location":{"id":"doi:10.64898/2026.11.04.764183","doi":"https://doi.org/10.64898/2026.11.04.764183","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.04.764183","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.11.04.764183","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5064002969","display_name":"Ankita Arora","orcid":"https://orcid.org/0000-0002-0279-8349","observed_orcids":["https://orcid.org/0000-0002-0279-8349"]},"institutions":[{"id":"https://openalex.org/I4210094671","display_name":"Regional Centre for Biotechnology","ror":"https://ror.org/00nc5f834","country_code":"IN","type":"government","lineage":["https://openalex.org/I4210094671"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Ankita Arora","raw_affiliation_strings":["UNECSO-DBT, Regional Centre for Biotechnology, Faridabad;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UNECSO-DBT, Regional Centre for Biotechnology, Faridabad;","institution_ids":["https://openalex.org/I4210094671"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5136553453","display_name":"Jhalak Aggarwal","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801880299","display_name":"Translational Health Science and Technology Institute","ror":"https://ror.org/01qjqvr92","country_code":"IN","type":"government","lineage":["https://openalex.org/I2799351866","https://openalex.org/I2801880299","https://openalex.org/I4210134808"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Jhalak Aggarwal","raw_affiliation_strings":["BRIC-Translational Health Science and Technology Institute, Faridabad;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BRIC-Translational Health Science and Technology Institute, Faridabad;","institution_ids":["https://openalex.org/I2801880299"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025854918","display_name":"Nisheeth Agarwal","orcid":"https://orcid.org/0000-0001-9203-3026","observed_orcids":["https://orcid.org/0000-0001-9203-3026"]},"institutions":[{"id":"https://openalex.org/I2801880299","display_name":"Translational Health Science and Technology Institute","ror":"https://ror.org/01qjqvr92","country_code":"IN","type":"government","lineage":["https://openalex.org/I2799351866","https://openalex.org/I2801880299","https://openalex.org/I4210134808"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Nisheeth Agarwal","raw_affiliation_strings":["Translational Health Science and Technology Institute;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Translational Health Science and Technology Institute;","institution_ids":["https://openalex.org/I2801880299"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003317190","display_name":"Prem Singh Kaushal","orcid":"https://orcid.org/0000-0001-6433-1368","observed_orcids":["https://orcid.org/0000-0001-6433-1368"]},"institutions":[{"id":"https://openalex.org/I4210094671","display_name":"Regional Centre for Biotechnology","ror":"https://ror.org/00nc5f834","country_code":"IN","type":"government","lineage":["https://openalex.org/I4210094671"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Prem S. Kaushal","raw_affiliation_strings":["UNECSO-DBT,  Regional Centre for Biotechnology, Faridabad"],"raw_orcid":"https://orcid.org/0000-0001-6433-1368","affiliations":[{"raw_affiliation_string":"UNECSO-DBT,  Regional Centre for Biotechnology, Faridabad","institution_ids":["https://openalex.org/I4210094671"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5003317190"],"corresponding_institution_ids":["https://openalex.org/I4210094671"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C69080955","wikidata":"https://www.wikidata.org/wiki/Q1088533","display_name":"50S","level":5,"score":0.9000999927520752},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.7962999939918518},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6416000127792358},{"id":"https://openalex.org/C52721335","wikidata":"https://www.wikidata.org/wiki/Q3305843","display_name":"30S","level":5,"score":0.5},{"id":"https://openalex.org/C2777204207","wikidata":"https://www.wikidata.org/wiki/Q606230","display_name":"Mycobacterium smegmatis","level":4,"score":0.44530001282691956},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4343000054359436},{"id":"https://openalex.org/C67905577","wikidata":"https://www.wikidata.org/wiki/Q215980","display_name":"Ribosomal RNA","level":3,"score":0.42419999837875366},{"id":"https://openalex.org/C129194359","wikidata":"https://www.wikidata.org/wiki/Q2636953","display_name":"Eukaryotic Ribosome","level":5,"score":0.4196999967098236},{"id":"https://openalex.org/C38062823","wikidata":"https://www.wikidata.org/wiki/Q3408228","display_name":"Ribosomal protein","level":5,"score":0.390500009059906},{"id":"https://openalex.org/C104292427","wikidata":"https://www.wikidata.org/wiki/Q899781","display_name":"Protein subunit","level":3,"score":0.387800008058548},{"id":"https://openalex.org/C191120209","wikidata":"https://www.wikidata.org/wiki/Q908902","display_name":"Structural biology","level":2,"score":0.3521000146865845},{"id":"https://openalex.org/C118716","wikidata":"https://www.wikidata.org/wiki/Q5514725","display_name":"GTP'","level":3,"score":0.3240000009536743},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.3163999915122986},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3057999908924103},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.2962999939918518},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.2630000114440918},{"id":"https://openalex.org/C96267730","wikidata":"https://www.wikidata.org/wiki/Q4647010","display_name":"A-site","level":3,"score":0.25760000944137573}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.11.04.764183","doi":"https://doi.org/10.64898/2026.11.04.764183","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.04.764183","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.11.04.764183","doi":"https://doi.org/10.64898/2026.11.04.764183","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.04.764183","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Ribosome":[0],"assembly,":[1],"a":[2,116,143,165],"highly":[3],"coordinated":[4,117],"and":[5,42,66,112,120,140,157],"crucial":[6],"cellular":[7],"process,":[8],"is":[9,22,124],"assisted":[10],"by":[11],"various":[12],"assembly":[13,26,122],"factors,":[14],"including":[15],"GTPases.":[16],"EngA,":[17],"an":[18,121],"essential":[19],"P-loop":[20],"GTPase,":[21],"involved":[23],"in":[24,37,72,94,126,154],"late-stage":[25],"of":[27,34,57,68,88,130,138],"the":[28,54,69,89,128,136],"50S":[29,35,47,61,70,92,113],"ribosomal":[30],"subunit.":[31],"The":[32],"structures":[33,67,87],"ribosome":[36,71,93,171],"complex":[38,73,95],"with":[39,74,96,135],"EngA-bound":[40,43,75,97,148],"GMPPNP,":[41],"GDP":[44,76,100,149],"alone,":[45],"without":[46],"subunit,":[48],"have":[49,77,83],"been":[50],"reported":[51,84],"previously.":[52],"However,":[53],"molecular":[55],"mechanism":[56],"EngA":[58,111],"GTP":[59],"hydrolysis-assisted":[60],"maturation":[62],"remains":[63],"poorly":[64],"understood,":[65],"yet":[78],"to":[79],"be":[80],"deciphered.":[81],"We":[82],"cryo-":[85],"EM":[86],"Mycobacterium":[90],"smegmatis":[91],"GTP\u03b3S":[98],"or":[99],"at":[101],"2.8":[102],"-":[103],"3.5":[104],"\u00c5":[105],"resolution.":[106],"Structural":[107],"analysis":[108],"revealed":[109],"that":[110],"subunit":[114],"undergo":[115],"structural":[118],"rearrangement,":[119],"intermediate":[123],"formed":[125],"which":[127],"stem-loop":[129],"H80":[131],"makes":[132],"base-pair":[133],"interactions":[134],"junction":[137],"H89":[139],"H90,":[141],"forming":[142],"unique":[144],"stem-loop-junction":[145],"(SLJ)":[146],"motif.":[147],"adopts":[150],"distinctly":[151],"different":[152],"conformations":[153],"its":[155],"ribosome-bound":[156],"unbound":[158],"states.":[159],"In":[160],"summary,":[161],"this":[162],"study":[163],"proposed":[164],"multi-state":[166],"conformational":[167],"model":[168],"for":[169],"EngA-assisted":[170],"assembly.":[172]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-11T00:00:00"}
