{"id":"https://openalex.org/W7225731485","doi":"https://doi.org/10.64898/2026.11.07.765024","title":"Distinct Roles of ADP and Magnesium in the Structural Remodeling and Higher-Order Assembly of Peroxiredoxin 2","display_name":"Distinct Roles of ADP and Magnesium in the Structural Remodeling and Higher-Order Assembly of Peroxiredoxin 2","publication_year":2026,"publication_date":"2026-11-10","ids":{"openalex":"https://openalex.org/W7225731485","doi":"https://doi.org/10.64898/2026.11.07.765024"},"language":"en","primary_location":{"id":"doi:10.64898/2026.11.07.765024","doi":"https://doi.org/10.64898/2026.11.07.765024","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.07.765024","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.64898/2026.11.07.765024","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014883969","display_name":"Tran Ngoc Trang","orcid":"https://orcid.org/0000-0002-7083-5770","observed_orcids":["https://orcid.org/0000-0002-7083-5770"]},"institutions":[{"id":"https://openalex.org/I10091056","display_name":"Kanazawa University","ror":"https://ror.org/02hwp6a56","country_code":"JP","type":"education","lineage":["https://openalex.org/I10091056"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tran Ngoc Trang","raw_affiliation_strings":["Kanazawa University;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kanazawa University;","institution_ids":["https://openalex.org/I10091056"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053263870","display_name":"Ryusei Yamada","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I10091056","display_name":"Kanazawa University","ror":"https://ror.org/02hwp6a56","country_code":"JP","type":"education","lineage":["https://openalex.org/I10091056"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Ryusei Yamada","raw_affiliation_strings":["Kanazawa University;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kanazawa University;","institution_ids":["https://openalex.org/I10091056"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113722080","display_name":"Kouki Morita","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I10091056","display_name":"Kanazawa University","ror":"https://ror.org/02hwp6a56","country_code":"JP","type":"education","lineage":["https://openalex.org/I10091056"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kouki Morita","raw_affiliation_strings":["Kanazawa University;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kanazawa University;","institution_ids":["https://openalex.org/I10091056"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5085945190","display_name":"Hiroki Konno","orcid":"https://orcid.org/0000-0002-1712-171X","observed_orcids":["https://orcid.org/0000-0002-1712-171X"]},"institutions":[{"id":"https://openalex.org/I10091056","display_name":"Kanazawa University","ror":"https://ror.org/02hwp6a56","country_code":"JP","type":"education","lineage":["https://openalex.org/I10091056"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroki Konno","raw_affiliation_strings":["Kanazawa university"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Kanazawa university","institution_ids":["https://openalex.org/I10091056"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I10091056"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.00839999970048666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.796999990940094},{"id":"https://openalex.org/C2780416994","wikidata":"https://www.wikidata.org/wiki/Q419462","display_name":"Peroxiredoxin","level":4,"score":0.6226000189781189},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5936999917030334},{"id":"https://openalex.org/C2775962898","wikidata":"https://www.wikidata.org/wiki/Q5073152","display_name":"Chaperone (clinical)","level":2,"score":0.4966000020503998},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.49160000681877136},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.46720001101493835},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4406000077724457},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4226999878883362},{"id":"https://openalex.org/C543218039","wikidata":"https://www.wikidata.org/wiki/Q660","display_name":"Magnesium","level":2,"score":0.4108000099658966},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.40950000286102295},{"id":"https://openalex.org/C2780642029","wikidata":"https://www.wikidata.org/wiki/Q726004","display_name":"Oligomer","level":2,"score":0.39959999918937683},{"id":"https://openalex.org/C2779637612","wikidata":"https://www.wikidata.org/wiki/Q2354337","display_name":"Phosphatidylserine","level":4,"score":0.38830000162124634},{"id":"https://openalex.org/C162008176","wikidata":"https://www.wikidata.org/wiki/Q419305","display_name":"Peroxidase","level":3,"score":0.37720000743865967},{"id":"https://openalex.org/C204328495","wikidata":"https://www.wikidata.org/wiki/Q847556","display_name":"Protein folding","level":2,"score":0.3474000096321106},{"id":"https://openalex.org/C102951782","wikidata":"https://www.wikidata.org/wiki/Q49295","display_name":"Atomic force microscopy","level":2,"score":0.3409999907016754},{"id":"https://openalex.org/C20702342","wikidata":"https://www.wikidata.org/wiki/Q5190506","display_name":"Cryo-electron microscopy","level":2,"score":0.29249998927116394},{"id":"https://openalex.org/C205260736","wikidata":"https://www.wikidata.org/wiki/Q418939","display_name":"Heat shock protein","level":3,"score":0.29159998893737793},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.27549999952316284},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.2648000121116638},{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.26460000872612},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2630000114440918}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.11.07.765024","doi":"https://doi.org/10.64898/2026.11.07.765024","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.07.765024","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.11.07.765024","doi":"https://doi.org/10.64898/2026.11.07.765024","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.07.765024","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"2-Cys":[0],"peroxiredoxins":[1],"(Prxs)":[2],"undergo":[3],"oligomeric":[4],"transitions":[5],"linked":[6],"to":[7,19,133],"peroxidase":[8],"and":[9,17,33,42,55,84,97,122,125],"chaperone":[10],"activities,":[11],"but":[12],"the":[13],"contributions":[14],"of":[15],"nucleotides":[16],"cations":[18],"lipid-dependent":[20],"assembly":[21],"remain":[22],"unclear.":[23],"We":[24],"examined":[25],"phosphatidylserine":[26],"(PS)-induced":[27],"Prx2":[28,46],"oligomers":[29],"using":[30],"biochemical":[31],"assays":[32],"high-speed":[34],"atomic":[35],"force":[36],"microscopy":[37],"(HS-AFM).":[38],"Both":[39],"wild-type":[40],"(WT)":[41],"P44A/S76A":[43],"mutant":[44],"(MT)":[45],"formed":[47],"chaperone-active":[48],"oligomers,":[49,65],"although":[50],"MT":[51],"assembled":[52],"more":[53,58],"slowly":[54],"exhibited":[56,94],"a":[57,130],"compact":[59],"lateral":[60],"geometry.":[61],"Mg":[62],"disassembled":[63,70],"both":[64,82],"whereas":[66],"ADP":[67],"alone":[68],"predominantly":[69],"WT.":[71],"Under":[72],"combined":[73],"Mg-ADP":[74],"conditions,":[75],"HS-AFM":[76],"directly":[77,128],"captured":[78],"oligomer":[79],"disassembly":[80],"in":[81],"proteins,":[83],"separate":[85],"experiments":[86],"demonstrated":[87],"higher-molecular-weight":[88],"(HMW)":[89],"assembly.":[90],"WT":[91],"HMW":[92,134],"assemblies":[93],"greater":[95],"heights":[96],"stronger":[98],"Mant-ADP":[99],"co-elution":[100],"than":[101],"MT.":[102],"Structural":[103],"predictions":[104],"suggested":[105],"altered":[106],"local":[107],"Ser76-mediated":[108],"contacts":[109],"without":[110,127],"major":[111],"backbone":[112],"changes.":[113],"These":[114],"results":[115],"distinguish":[116],"PS-induced":[117],"functional":[118],"switching":[119],"from":[120],"Mg-":[121],"ADP-dependent":[123],"remodeling":[124],"support,":[126],"proving,":[129],"disassembly-reassembly":[131],"route":[132],"formation.":[135]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-11T00:00:00"}