{"id":"https://openalex.org/W7225729564","doi":"https://doi.org/10.5281/zenodo.24109035","title":"Cell-Free DNA Circuits as the Safer Injectable: A Design Study of a Marker-Activated Molecular System Without Living Cells","display_name":"Cell-Free DNA Circuits as the Safer Injectable: A Design Study of a Marker-Activated Molecular System Without Living Cells","publication_year":2026,"publication_date":"2026-11-10","ids":{"openalex":"https://openalex.org/W7225729564","doi":"https://doi.org/10.5281/zenodo.24109035"},"language":"en","primary_location":{"id":"doi:10.5281/zenodo.24109035","doi":"https://doi.org/10.5281/zenodo.24109035","is_oa":true,"landing_page_url":"https://doi.org/10.5281/zenodo.24109035","pdf_url":null,"source":{"id":"https://openalex.org/S4306400562","display_name":"Zenodo (CERN European Organization for Nuclear Research)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I67311998","host_organization_name":"European Organization for Nuclear Research","host_organization_lineage":["https://openalex.org/I67311998"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Preprint"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.5281/zenodo.24109035","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5161362375","display_name":"William Alexander Aldred","orcid":"https://orcid.org/0009-0000-1215-577X","observed_orcids":["https://orcid.org/0009-0000-1215-577X"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Aldred, William Alexander","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0009-0000-1215-577X","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5161362375"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9146000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9146000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12029","display_name":"DNA and Biological Computing","score":0.04639999940991402,"subfield":{"id":"https://openalex.org/subfields/2614","display_name":"Theoretical Computer Science"},"field":{"id":"https://openalex.org/fields/26","display_name":"Mathematics"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.006300000008195639,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C134066672","wikidata":"https://www.wikidata.org/wiki/Q1424639","display_name":"Payload (computing)","level":3,"score":0.6107000112533569},{"id":"https://openalex.org/C2776654903","wikidata":"https://www.wikidata.org/wiki/Q2601463","display_name":"SAFER","level":2,"score":0.5752999782562256},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5597000122070312},{"id":"https://openalex.org/C2779696439","wikidata":"https://www.wikidata.org/wiki/Q7512811","display_name":"Signature (topology)","level":2,"score":0.4494999945163727},{"id":"https://openalex.org/C2776184637","wikidata":"https://www.wikidata.org/wiki/Q6506039","display_name":"Living systems","level":2,"score":0.438400000333786},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4088999927043915},{"id":"https://openalex.org/C2984801823","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Living cell","level":2,"score":0.40230000019073486},{"id":"https://openalex.org/C2780428219","wikidata":"https://www.wikidata.org/wiki/Q16952335","display_name":"Cover (algebra)","level":2,"score":0.3481000065803528},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.34470000863075256},{"id":"https://openalex.org/C134146338","wikidata":"https://www.wikidata.org/wiki/Q1815901","display_name":"Electronic circuit","level":2,"score":0.34439998865127563},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3433000147342682},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.34290000796318054},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3400999903678894},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.2985999882221222},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2939999997615814},{"id":"https://openalex.org/C31352089","wikidata":"https://www.wikidata.org/wiki/Q3750474","display_name":"Systems design","level":2,"score":0.2921999990940094},{"id":"https://openalex.org/C143425029","wikidata":"https://www.wikidata.org/wiki/Q5205747","display_name":"DNA damage","level":3,"score":0.29089999198913574},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.2874999940395355},{"id":"https://openalex.org/C33276779","wikidata":"https://www.wikidata.org/wiki/Q1943363","display_name":"Design elements and principles","level":2,"score":0.27160000801086426},{"id":"https://openalex.org/C26517878","wikidata":"https://www.wikidata.org/wiki/Q228039","display_name":"Key (lock)","level":2,"score":0.26930001378059387},{"id":"https://openalex.org/C154256306","wikidata":"https://www.wikidata.org/wiki/Q41795528","display_name":"Inert","level":2,"score":0.2648000121116638},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.26170000433921814},{"id":"https://openalex.org/C191961535","wikidata":"https://www.wikidata.org/wiki/Q2360205","display_name":"A-DNA","level":3,"score":0.2614000141620636},{"id":"https://openalex.org/C190560348","wikidata":"https://www.wikidata.org/wiki/Q3245116","display_name":"Circuit design","level":2,"score":0.25780001282691956},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.2540999948978424},{"id":"https://openalex.org/C3018877446","wikidata":"https://www.wikidata.org/wiki/Q1421432","display_name":"Disease monitoring","level":3,"score":0.25290000438690186}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.5281/zenodo.24109035","doi":"https://doi.org/10.5281/zenodo.24109035","is_oa":true,"landing_page_url":"https://doi.org/10.5281/zenodo.24109035","pdf_url":null,"source":{"id":"https://openalex.org/S4306400562","display_name":"Zenodo (CERN European Organization for Nuclear Research)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I67311998","host_organization_name":"European Organization for Nuclear Research","host_organization_lineage":["https://openalex.org/I67311998"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Preprint"},{"id":"doi:10.5281/zenodo.24109036","doi":"https://doi.org/10.5281/zenodo.24109036","is_oa":true,"landing_page_url":"https://doi.org/10.5281/zenodo.24109036","pdf_url":null,"source":{"id":"https://openalex.org/S4306400562","display_name":"Zenodo (CERN European Organization for Nuclear Research)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I67311998","host_organization_name":"European Organization for Nuclear Research","host_organization_lineage":["https://openalex.org/I67311998"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Preprint"}],"best_oa_location":{"id":"doi:10.5281/zenodo.24109035","doi":"https://doi.org/10.5281/zenodo.24109035","is_oa":true,"landing_page_url":"https://doi.org/10.5281/zenodo.24109035","pdf_url":null,"source":{"id":"https://openalex.org/S4306400562","display_name":"Zenodo (CERN European Organization for Nuclear Research)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I67311998","host_organization_name":"European Organization for Nuclear Research","host_organization_lineage":["https://openalex.org/I67311998"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Preprint"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":[],"abstract_inverted_index":{"Design":[0],"study":[1],"of":[2,11,84],"an":[3],"injectable":[4],"diagnostic":[5],"and":[6,22,60,81,89],"therapeutic-logic":[7],"system":[8],"made":[9],"only":[10,24],"DNA":[12],"strands,":[13],"with":[14,72],"no":[15],"living":[16,78],"cells.":[17],"The":[18],"circuit":[19],"circulates":[20],"inert":[21],"activates":[23],"inside":[25],"cells":[26],"presenting":[27],"a":[28,41,49,68,73],"two-marker":[29],"disease":[30],"signature":[31],"via":[32],"toehold":[33],"exchange.":[34],"Computed":[35],"budgets":[36],"cover":[37],"activation":[38,47],"(0.510":[39],"in":[40,48],"diseased":[42],"cell,":[43],"3.66%":[44],"total":[45],"false":[46],"healthy":[50],"cell),":[51],"clearance":[52],"(4.36":[53],"h":[54,57],"half-life,":[55],"14.5":[56],"working":[58],"window),":[59],"payload":[61],"(35,075":[62],"copies":[63],"per":[64],"target":[65],"cell":[66],"against":[67,77],"10,000-copy":[69],"requirement),":[70],"together":[71],"quantitative":[74],"safety":[75],"comparison":[76],"engineered-cell":[79],"products":[80],"the":[82],"costs":[83],"giving":[85],"up":[86],"replication,":[87],"migration,":[88],"memory.":[90],"SIMULATED,":[91],"NOT":[92],"MEASURED.":[93],"Nothing":[94],"described":[95],"has":[96],"been":[97],"built,":[98],"synthesized,":[99],"or":[100],"tested.":[101]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-11T00:00:00"}