{"id":"https://openalex.org/W7225652248","doi":"https://doi.org/10.21203/rs.3.rs-11476429/v1","title":"Role of nitrate-dependent anaerobic methanotrophs in reducing methane emissions under delayed flooding in straw-amended paddy soil","display_name":"Role of nitrate-dependent anaerobic methanotrophs in reducing methane emissions under delayed flooding in straw-amended paddy soil","publication_year":2026,"publication_date":"2026-11-09","ids":{"openalex":"https://openalex.org/W7225652248","doi":"https://doi.org/10.21203/rs.3.rs-11476429/v1"},"language":"en","primary_location":{"id":"doi:10.21203/rs.3.rs-11476429/v1","doi":"https://doi.org/10.21203/rs.3.rs-11476429/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11476429/v1","pdf_url":"https://www.researchsquare.com/article/rs-11476429/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.researchsquare.com/article/rs-11476429/latest.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5161309245","display_name":"Duyang Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177739611","display_name":"Yangtze University","ror":"https://ror.org/05bhmhz54","country_code":"CN","type":"education","lineage":["https://openalex.org/I177739611"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Duyang Wang","raw_affiliation_strings":["Yangtze University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yangtze University","institution_ids":["https://openalex.org/I177739611"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040690273","display_name":"Xinyu Yan","orcid":"https://orcid.org/0009-0004-5746-5180","observed_orcids":["https://orcid.org/0009-0004-5746-5180"]},"institutions":[{"id":"https://openalex.org/I177739611","display_name":"Yangtze University","ror":"https://ror.org/05bhmhz54","country_code":"CN","type":"education","lineage":["https://openalex.org/I177739611"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xinyu Yan","raw_affiliation_strings":["Yangtze University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yangtze University","institution_ids":["https://openalex.org/I177739611"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100396274","display_name":"Peng Xu","orcid":"https://orcid.org/0009-0003-8801-2347","observed_orcids":["https://orcid.org/0009-0003-8801-2347"]},"institutions":[{"id":"https://openalex.org/I177739611","display_name":"Yangtze University","ror":"https://ror.org/05bhmhz54","country_code":"CN","type":"education","lineage":["https://openalex.org/I177739611"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Peng Xu","raw_affiliation_strings":["Yangtze University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yangtze University","institution_ids":["https://openalex.org/I177739611"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161346432","display_name":"Wei Liu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177739611","display_name":"Yangtze University","ror":"https://ror.org/05bhmhz54","country_code":"CN","type":"education","lineage":["https://openalex.org/I177739611"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wei Liu","raw_affiliation_strings":["Yangtze University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yangtze University","institution_ids":["https://openalex.org/I177739611"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100396520","display_name":"Lu Liu","orcid":"https://orcid.org/0000-0002-8949-9105","observed_orcids":["https://orcid.org/0000-0002-8949-9105"]},"institutions":[{"id":"https://openalex.org/I177739611","display_name":"Yangtze University","ror":"https://ror.org/05bhmhz54","country_code":"CN","type":"education","lineage":["https://openalex.org/I177739611"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lu Liu","raw_affiliation_strings":["Yangtze University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yangtze University","institution_ids":["https://openalex.org/I177739611"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161307158","display_name":"Mengdie D. Jiang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I177739611","display_name":"Yangtze University","ror":"https://ror.org/05bhmhz54","country_code":"CN","type":"education","lineage":["https://openalex.org/I177739611"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Mengdie D. Jiang","raw_affiliation_strings":["Yangtze University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yangtze University","institution_ids":["https://openalex.org/I177739611"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5159068401","display_name":"Tingyan Zhang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Tingyan Zhang","raw_affiliation_strings":["Huazhong Agricultural University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Huazhong Agricultural University","institution_ids":["https://openalex.org/I204823248"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5161316909","display_name":"Yong Zhou","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210154338","display_name":"Hubei Engineering University","ror":"https://ror.org/05amnwk22","country_code":"CN","type":"education","lineage":["https://openalex.org/I4210154338"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yong Zhou","raw_affiliation_strings":["Hubei Engineering University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hubei Engineering University","institution_ids":["https://openalex.org/I4210154338"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10004","display_name":"Soil Carbon and Nitrogen Dynamics","score":0.6298999786376953,"subfield":{"id":"https://openalex.org/subfields/1111","display_name":"Soil Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10004","display_name":"Soil Carbon and Nitrogen Dynamics","score":0.6298999786376953,"subfield":{"id":"https://openalex.org/subfields/1111","display_name":"Soil Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12091","display_name":"Peatlands and Wetlands Ecology","score":0.22920000553131104,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10995","display_name":"Methane Hydrates and Related Phenomena","score":0.037300001829862595,"subfield":{"id":"https://openalex.org/subfields/2304","display_name":"Environmental Chemistry"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/methane-emissions","display_name":"methane emissions","score":0.9190000295639038},{"id":"https://openalex.org/keywords/straw-incorporation","display_name":"straw incorporation","score":0.9120000004768372},{"id":"https://openalex.org/keywords/paddy-soil","display_name":"paddy soil","score":0.8849999904632568},{"id":"https://openalex.org/keywords/methanogenic-archaea","display_name":"methanogenic archaea","score":0.7350000143051147},{"id":"https://openalex.org/keywords/maize-straw","display_name":"maize straw","score":0.5339999794960022},{"id":"https://openalex.org/keywords/nitrogen-availability","display_name":"nitrogen availability","score":0.4410000145435333},{"id":"https://openalex.org/keywords/paddy-fields","display_name":"paddy fields","score":0.7889999747276306},{"id":"https://openalex.org/keywords/methane-mitigation","display_name":"methane mitigation","score":0.6589999794960022}],"concepts":[{"id":"https://openalex.org/C2779587293","wikidata":"https://www.wikidata.org/wiki/Q7622360","display_name":"Straw","level":2,"score":0.5676000118255615},{"id":"https://openalex.org/C180553826","wikidata":"https://www.wikidata.org/wiki/Q3333382","display_name":"Mesocosm","level":3,"score":0.5145999789237976},{"id":"https://openalex.org/C2777438225","wikidata":"https://www.wikidata.org/wiki/Q1169785","display_name":"Methanogen","level":3,"score":0.5073000192642212},{"id":"https://openalex.org/C6557445","wikidata":"https://www.wikidata.org/wiki/Q173113","display_name":"Agronomy","level":1,"score":0.504800021648407},{"id":"https://openalex.org/C39432304","wikidata":"https://www.wikidata.org/wiki/Q188847","display_name":"Environmental science","level":0,"score":0.4839000105857849},{"id":"https://openalex.org/C2777671786","wikidata":"https://www.wikidata.org/wiki/Q2633954","display_name":"Methanotroph","level":4,"score":0.4507000148296356},{"id":"https://openalex.org/C77077793","wikidata":"https://www.wikidata.org/wiki/Q336019","display_name":"Abundance (ecology)","level":2,"score":0.44670000672340393},{"id":"https://openalex.org/C186594467","wikidata":"https://www.wikidata.org/wiki/Q1429176","display_name":"Flooding (psychology)","level":2,"score":0.43160000443458557},{"id":"https://openalex.org/C516920438","wikidata":"https://www.wikidata.org/wiki/Q37129","display_name":"Methane","level":2,"score":0.38519999384880066},{"id":"https://openalex.org/C2992001702","wikidata":"https://www.wikidata.org/wiki/Q160066","display_name":"Rice straw","level":3,"score":0.359499990940094},{"id":"https://openalex.org/C115540264","wikidata":"https://www.wikidata.org/wiki/Q2945560","display_name":"Biomass (ecology)","level":2,"score":0.35190001130104065},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3393999934196472},{"id":"https://openalex.org/C122325731","wikidata":"https://www.wikidata.org/wiki/Q3738459","display_name":"Relative species abundance","level":3,"score":0.3314000070095062},{"id":"https://openalex.org/C107872376","wikidata":"https://www.wikidata.org/wiki/Q321355","display_name":"Environmental chemistry","level":1,"score":0.32019999623298645},{"id":"https://openalex.org/C140793950","wikidata":"https://www.wikidata.org/wiki/Q168091","display_name":"Animal science","level":1,"score":0.30959999561309814},{"id":"https://openalex.org/C53991642","wikidata":"https://www.wikidata.org/wiki/Q128991","display_name":"Biofuel","level":2,"score":0.2994000017642975},{"id":"https://openalex.org/C159750122","wikidata":"https://www.wikidata.org/wiki/Q96621023","display_name":"Soil water","level":2,"score":0.2939000129699707},{"id":"https://openalex.org/C156380964","wikidata":"https://www.wikidata.org/wiki/Q18556","display_name":"Bioenergy","level":3,"score":0.2921999990940094},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.28940001130104065},{"id":"https://openalex.org/C38774213","wikidata":"https://www.wikidata.org/wiki/Q202080","display_name":"Soil fertility","level":3,"score":0.28760001063346863},{"id":"https://openalex.org/C104577883","wikidata":"https://www.wikidata.org/wiki/Q4065016","display_name":"Anaerobic oxidation of methane","level":3,"score":0.2840000092983246},{"id":"https://openalex.org/C2776208129","wikidata":"https://www.wikidata.org/wiki/Q30672844","display_name":"Methane emissions","level":3,"score":0.2784999907016754},{"id":"https://openalex.org/C2778589620","wikidata":"https://www.wikidata.org/wiki/Q22275999","display_name":"Methanogenesis","level":3,"score":0.27000001072883606},{"id":"https://openalex.org/C172365310","wikidata":"https://www.wikidata.org/wiki/Q7554937","display_name":"Soil microbiology","level":3,"score":0.26910001039505005},{"id":"https://openalex.org/C5140985","wikidata":"https://www.wikidata.org/wiki/Q483866","display_name":"Anaerobic exercise","level":2,"score":0.2685000002384186},{"id":"https://openalex.org/C175605896","wikidata":"https://www.wikidata.org/wiki/Q39833","display_name":"Microorganism","level":3,"score":0.2605000138282776},{"id":"https://openalex.org/C81407943","wikidata":"https://www.wikidata.org/wiki/Q17149178","display_name":"Microbial population biology","level":3,"score":0.2581999897956848},{"id":"https://openalex.org/C85582077","wikidata":"https://www.wikidata.org/wiki/Q842623","display_name":"Paddy field","level":2,"score":0.2565000057220459}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21203/rs.3.rs-11476429/v1","doi":"https://doi.org/10.21203/rs.3.rs-11476429/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11476429/v1","pdf_url":"https://www.researchsquare.com/article/rs-11476429/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.21203/rs.3.rs-11476429/v1","doi":"https://doi.org/10.21203/rs.3.rs-11476429/v1","is_oa":true,"landing_page_url":"https://doi.org/10.21203/rs.3.rs-11476429/v1","pdf_url":"https://www.researchsquare.com/article/rs-11476429/latest.pdf","source":{"id":"https://openalex.org/S4306525896","display_name":"Research Square","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Research Square","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/13","score":0.9937000274658203,"display_name":"Climate action"},{"id":"https://metadata.un.org/sdg/2","score":0.5666999816894531,"display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320322186","display_name":"Natural Science Foundation of Hubei Province","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225652248.pdf","grobid_xml":"https://content.openalex.org/works/W7225652248.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W1496450599","https://openalex.org/W1836101600","https://openalex.org/W1964048070","https://openalex.org/W1982200102","https://openalex.org/W2011379546","https://openalex.org/W2017367256","https://openalex.org/W2025408874","https://openalex.org/W2028364867","https://openalex.org/W2056257661","https://openalex.org/W2066790073","https://openalex.org/W2070868107","https://openalex.org/W2100643889","https://openalex.org/W2115789299","https://openalex.org/W2128773885","https://openalex.org/W2171619585","https://openalex.org/W2520751773","https://openalex.org/W2886034144","https://openalex.org/W2919972249","https://openalex.org/W2964393761","https://openalex.org/W2990918882","https://openalex.org/W3097092098","https://openalex.org/W3171944751","https://openalex.org/W4283068459","https://openalex.org/W4367310556","https://openalex.org/W4387054074","https://openalex.org/W4390635988","https://openalex.org/W4390818168","https://openalex.org/W4403194336","https://openalex.org/W4404500080","https://openalex.org/W4407205507","https://openalex.org/W4408308539","https://openalex.org/W4410190584","https://openalex.org/W4414817818","https://openalex.org/W4415015298","https://openalex.org/W4416206827","https://openalex.org/W7116875513","https://openalex.org/W7162092242","https://openalex.org/W7204435398"],"related_works":["https://openalex.org/W7124433491","https://openalex.org/W2291068506","https://openalex.org/W4402042448","https://openalex.org/W2793534138","https://openalex.org/W3025132070","https://openalex.org/W4317039023","https://openalex.org/W3033896013","https://openalex.org/W2004274341","https://openalex.org/W2989672567","https://openalex.org/W3172378780"],"abstract_inverted_index":{"Abstract":[0],"Straw":[1],"incorporation":[2,51],"is":[3,28],"commonly":[4],"adopted":[5],"to":[6,16,103,195,266],"improve":[7],"soil":[8],"fertility":[9],"in":[10,19,99,116,271],"rice":[11],"paddies,":[12],"yet":[13],"it":[14],"leads":[15],"substantial":[17],"increases":[18],"methane":[20],"(CH":[21],"4":[22,42,93,110,155,186,229,269],")":[23],"emissions.":[24,187,220],"Although":[25],"water":[26,79],"management":[27],"recognized":[29],"as":[30,180,262],"an":[31,263],"effective":[32,264],"mitigation":[33],"strategy,":[34],"the":[35,45,181,226],"impacts":[36],"of":[37,184],"delayed":[38,83,106,233,259],"flooding":[39,107,124,234,260],"on":[40,147],"CH":[41,92,109,154,185,228,268],"emissions":[43,111,156,230,270],"and":[44,74,77,82,114,118,129,164,205],"underlying":[46],"microbial":[47],"mechanisms":[48],"under":[49,235],"straw":[50,68,88,236],"remain":[52],"poorly":[53],"understood.":[54],"To":[55],"fill":[56],"this":[57],"knowledge":[58],"gap,":[59],"a":[60],"laboratory":[61],"mesocosm":[62],"study":[63],"was":[64,238],"conducted":[65],"involving":[66],"three":[67],"treatments":[69],"(no":[70],"straw,":[71],"maize":[72,75],"leaf,":[73],"stem)":[76],"two":[78],"regimes":[80],"(direct":[81],"flooding).":[84],"Results":[85],"showed":[86],"that":[87,225,258],"addition":[89,237],"significantly":[90,125],"increased":[91,136],"emissions,":[94,198],"with":[95,143,160,167,217],"greater":[96,206],"stimulation":[97],"observed":[98],"leaf-amended":[100],"soil.":[101],"Relative":[102],"direct":[104],"flooding,":[105],"reduced":[108,227],"by":[112,232,241],"83.3%":[113],"85.6%":[115],"leaf-":[117],"stem-":[119],"amended":[120],"soils,":[121],"respectively.":[122],"Delayed":[123],"decreased":[126],"methanogenic":[127,161,177,192],"mcrA":[128,141,162,172,178,193,211],"aerobic":[130],"methanotrophic":[131],"pmoA":[132,152],"gene":[133,212],"abundances":[134],"but":[135],"M.":[137,148,168,207,250],"nitroreducens":[138,169,208,251],"-like":[139,150,170,209,252],"archaeal":[140,171,210],"abundance,":[142],"no":[144],"significant":[145],"effect":[146],"oxyfera":[149],"bacterial":[151],".":[153],"were":[157,214],"positively":[158],"associated":[159,166,216],"abundance":[163,179,194,213],"negatively":[165],"abundance.":[173],"Random":[174],"forest":[175],"identified":[176],"strongest":[182],"predictor":[183],"SHAP":[188],"analysis":[189,222],"linked":[190],"higher":[191,196,200],"predicted":[197,219],"whereas":[199],"NO":[201,243],"3":[202,244],"\u207b-N":[203,245],"content":[204],"generally":[215],"lower":[218],"PLS-PM":[221],"further":[223],"revealed":[224],"caused":[231],"primarily":[239],"mediated":[240],"increasing":[242],"content,":[246],"which":[247],"subsequently":[248],"stimulated":[249],"archaea.":[253],"Collectively,":[254],"our":[255],"findings":[256],"demonstrate":[257],"serves":[261],"strategy":[265],"mitigate":[267],"straw-incorporated":[272],"paddy":[273],"ecosystems.":[274]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-10T00:00:00"}