{"id":"https://openalex.org/W7225645957","doi":"https://doi.org/10.3389/fgene.2026.1958697","title":"A forward genetics workflow for EMS mutagenesis, agar-based screening, and genomic analysis of Macrocystis pyrifera gametophytes","display_name":"A forward genetics workflow for EMS mutagenesis, agar-based screening, and genomic analysis of Macrocystis pyrifera gametophytes","publication_year":2026,"publication_date":"2026-11-09","ids":{"openalex":"https://openalex.org/W7225645957","doi":"https://doi.org/10.3389/fgene.2026.1958697"},"language":"en","primary_location":{"id":"doi:10.3389/fgene.2026.1958697","doi":"https://doi.org/10.3389/fgene.2026.1958697","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fgene.2026.1958697","pdf_url":"https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2026.1958697/pdf","source":{"id":"https://openalex.org/S2597340651","display_name":"Frontiers in Genetics","issn_l":"1664-8021","issn":["1664-8021"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Genetics","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2026.1958697/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5072383749","display_name":"Brandon Vong","orcid":"https://orcid.org/0000-0001-8278-1469","observed_orcids":["https://orcid.org/0000-0001-8278-1469"]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brandon Vong","raw_affiliation_strings":["Department of Molecular and Cellular Biosciences, University of Southern California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cellular Biosciences, University of Southern California","institution_ids":["https://openalex.org/I1174212"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101202906","display_name":"Zhihan Hua","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zhihan Hua","raw_affiliation_strings":["Department of Molecular and Cellular Biosciences, University of Southern California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cellular Biosciences, University of Southern California","institution_ids":["https://openalex.org/I1174212"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161316220","display_name":"Isabella Langa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Isabella Langa","raw_affiliation_strings":["Department of Molecular and Cellular Biosciences, University of Southern California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cellular Biosciences, University of Southern California","institution_ids":["https://openalex.org/I1174212"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161333934","display_name":"Jack Monney","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jack Monney","raw_affiliation_strings":["Department of Molecular and Cellular Biosciences, University of Southern California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cellular Biosciences, University of Southern California","institution_ids":["https://openalex.org/I1174212"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5026445054","display_name":"Sergey V. Nuzhdin","orcid":"https://orcid.org/0000-0002-9963-151X","observed_orcids":["https://orcid.org/0000-0002-9963-151X"]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Sergey Nuzhdin","raw_affiliation_strings":["Department of Molecular and Cellular Biosciences, University of Southern California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular and Cellular Biosciences, University of Southern California","institution_ids":["https://openalex.org/I1174212"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5026445054"],"corresponding_institution_ids":["https://openalex.org/I1174212"],"apc_list":{"value":3150,"currency":"CHF","value_usd":3801},"apc_paid":{"value":3150,"currency":"CHF","value_usd":3801},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"17","issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12828","display_name":"Plant Genetic and Mutation Studies","score":0.9639000296592712,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12828","display_name":"Plant Genetic and Mutation Studies","score":0.9639000296592712,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10643","display_name":"Marine and coastal plant biology","score":0.025200000032782555,"subfield":{"id":"https://openalex.org/subfields/1910","display_name":"Oceanography"},"field":{"id":"https://openalex.org/fields/19","display_name":"Earth and Planetary Sciences"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11390","display_name":"Seaweed-derived Bioactive Compounds","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1104","display_name":"Aquatic Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/macrocystis-pyrifera","display_name":"Macrocystis pyrifera","score":1.0},{"id":"https://openalex.org/keywords/gametophyte","display_name":"gametophyte","score":0.9649999737739563},{"id":"https://openalex.org/keywords/forward-genetics","display_name":"forward genetics","score":0.847000002861023},{"id":"https://openalex.org/keywords/ems-mutagenesis","display_name":"EMS mutagenesis","score":0.9160000085830688},{"id":"https://openalex.org/keywords/chemical-mutagenesis","display_name":"chemical mutagenesis","score":0.7960000038146973},{"id":"https://openalex.org/keywords/strain-isolation","display_name":"strain isolation","score":0.8410000205039978},{"id":"https://openalex.org/keywords/whole-genome-sequencing","display_name":"whole genome sequencing","score":0.7350000143051147}],"concepts":[{"id":"https://openalex.org/C2779110813","wikidata":"https://www.wikidata.org/wiki/Q278392","display_name":"Macrocystis pyrifera","level":3,"score":0.9330999851226807},{"id":"https://openalex.org/C148885314","wikidata":"https://www.wikidata.org/wiki/Q189951","display_name":"Gametophyte","level":3,"score":0.9136000275611877},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7013999819755554},{"id":"https://openalex.org/C2779115727","wikidata":"https://www.wikidata.org/wiki/Q416643","display_name":"Ethyl methanesulfonate","level":4,"score":0.6283000111579895},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5511999726295471},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5397999882698059},{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.4440000057220459},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.436599999666214},{"id":"https://openalex.org/C2776639595","wikidata":"https://www.wikidata.org/wiki/Q221696","display_name":"Mutagen","level":3,"score":0.42410001158714294},{"id":"https://openalex.org/C36723025","wikidata":"https://www.wikidata.org/wiki/Q3330905","display_name":"Mutation breeding","level":4,"score":0.3465999960899353},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.2994000017642975},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.29409998655319214},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.2928999960422516},{"id":"https://openalex.org/C24432333","wikidata":"https://www.wikidata.org/wiki/Q2068526","display_name":"Whole genome sequencing","level":4,"score":0.2874999940395355},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.275299996137619},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.26910001039505005},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.2671999931335449},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2614000141620636},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.25110000371932983}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3389/fgene.2026.1958697","doi":"https://doi.org/10.3389/fgene.2026.1958697","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fgene.2026.1958697","pdf_url":"https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2026.1958697/pdf","source":{"id":"https://openalex.org/S2597340651","display_name":"Frontiers in Genetics","issn_l":"1664-8021","issn":["1664-8021"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Genetics","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.3389/fgene.2026.1958697","doi":"https://doi.org/10.3389/fgene.2026.1958697","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fgene.2026.1958697","pdf_url":"https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2026.1958697/pdf","source":{"id":"https://openalex.org/S2597340651","display_name":"Frontiers in Genetics","issn_l":"1664-8021","issn":["1664-8021"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Genetics","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320308668","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60"},{"id":"https://openalex.org/F4320309030","display_name":"Small Business Innovation Research","ror":"https://ror.org/015t55b95"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225645957.pdf","grobid_xml":"https://content.openalex.org/works/W7225645957.grobid-xml"},"referenced_works_count":18,"referenced_works":["https://openalex.org/W2093662362","https://openalex.org/W2106578986","https://openalex.org/W2161950199","https://openalex.org/W2169123200","https://openalex.org/W2790260691","https://openalex.org/W2951912016","https://openalex.org/W3047561308","https://openalex.org/W3131055609","https://openalex.org/W3165356543","https://openalex.org/W4206318133","https://openalex.org/W4366264269","https://openalex.org/W4386712380","https://openalex.org/W4394666350","https://openalex.org/W4396579048","https://openalex.org/W4406850209","https://openalex.org/W4410379560","https://openalex.org/W7117578233","https://openalex.org/W7155218392"],"related_works":["https://openalex.org/W3206827014","https://openalex.org/W3000749762","https://openalex.org/W2741347150","https://openalex.org/W2758305038","https://openalex.org/W1570228804","https://openalex.org/W4411035710","https://openalex.org/W4414480444","https://openalex.org/W2081934519","https://openalex.org/W7154963087","https://openalex.org/W341912221"],"abstract_inverted_index":{"In":[0,77],"this":[1,20,78],"study":[2],"we":[3],"establish":[4],"a":[5,10,85],"proof-of-concept":[6],"workflow":[7],"for":[8,56,88,97],"conducting":[9],"mutagenesis":[11],"screen":[12],"in":[13],"Macrocystis":[14],"pyrifera":[15,95],"g":[16],"ametophytes.":[17],"We":[18,44],"accomplish":[19],"by":[21],"treating":[22],"the":[23,47],"gametophytes":[24,49,96],"with":[25,74],"chemical":[26],"mutagen":[27],"ethyl":[28],"methanesulfonate":[29],"(EMS),":[30],"which":[31],"is":[32,82],"known":[33],"to":[34,53],"induce":[35],"G":[36,65],">":[37,41,66,70],"A":[38,67],"and":[39,59,68,91],"C":[40,69],"T":[42,71],"transitions.":[43],"then":[45],"culture":[46,81],"EMS-treated":[48],"on":[50],"solid":[51],"media":[52],"immobilize":[54],"them":[55],"streamlined":[57],"screening":[58],"strain":[60],"isolation.":[61],"Whole-genome":[62],"sequencing":[63],"identified":[64],"substitutions":[72],"consistent":[73],"EMS":[75],"mutagenesis.":[76],"workflow,":[79],"agar-based":[80],"used":[83],"as":[84],"practical":[86],"tool":[87],"immobilizing,":[89],"screening,":[90],"recovering":[92],"mutagenized":[93],"M.":[94],"downstream":[98],"analysis.":[99]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-10T00:00:00"}