{"id":"https://openalex.org/W7225639339","doi":"https://doi.org/10.1002/bmc.70658","title":"Sensitive LC\u2013MS/MS Method for Simultaneous Quantification of Alectinib and Its Major Metabolite M4 in Limited\u2010Volume Human Plasma","display_name":"Sensitive LC\u2013MS/MS Method for Simultaneous Quantification of Alectinib and Its Major Metabolite M4 in Limited\u2010Volume Human Plasma","publication_year":2026,"publication_date":"2026-11-08","ids":{"openalex":"https://openalex.org/W7225639339","doi":"https://doi.org/10.1002/bmc.70658","pmid":"https://pubmed.ncbi.nlm.nih.gov/43000705"},"language":"en","primary_location":{"id":"doi:10.1002/bmc.70658","doi":"https://doi.org/10.1002/bmc.70658","is_oa":true,"landing_page_url":"https://doi.org/10.1002/bmc.70658","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bmc.70658","source":{"id":"https://openalex.org/S123979729","display_name":"Biomedical Chromatography","issn_l":"0269-3879","issn":["0269-3879","1099-0801"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical Chromatography","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bmc.70658","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087090928","display_name":"Reiko Makihara Ando","orcid":"https://orcid.org/0000-0001-5074-5690","observed_orcids":["https://orcid.org/0000-0001-5074-5690"]},"institutions":[{"id":"https://openalex.org/I4405285273","display_name":"National Cancer Center Hospital","ror":"https://ror.org/053hhpd64","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210087348","https://openalex.org/I4405285273"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Reiko Ando Makihara","raw_affiliation_strings":["Department of Pharmacy National Cancer Center Hospital  Tokyo Japan"],"raw_orcid":"https://orcid.org/0000-0001-5074-5690","affiliations":[{"raw_affiliation_string":"Department of Pharmacy National Cancer Center Hospital  Tokyo Japan","institution_ids":["https://openalex.org/I4405285273"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161324676","display_name":"Makoto Maeda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405285273","display_name":"National Cancer Center Hospital","ror":"https://ror.org/053hhpd64","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210087348","https://openalex.org/I4405285273"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Makoto Maeda","raw_affiliation_strings":["Department of Pharmacy National Cancer Center Hospital  Tokyo Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacy National Cancer Center Hospital  Tokyo Japan","institution_ids":["https://openalex.org/I4405285273"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067177354","display_name":"Ayumu Arakawa","orcid":"https://orcid.org/0009-0007-6501-7814","observed_orcids":["https://orcid.org/0009-0007-6501-7814"]},"institutions":[{"id":"https://openalex.org/I4405285273","display_name":"National Cancer Center Hospital","ror":"https://ror.org/053hhpd64","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210087348","https://openalex.org/I4405285273"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Ayumu Arakawa","raw_affiliation_strings":["Department of Pediatric Oncology National Cancer Center Hospital  Tokyo Japan"],"raw_orcid":"https://orcid.org/0009-0007-6501-7814","affiliations":[{"raw_affiliation_string":"Department of Pediatric Oncology National Cancer Center Hospital  Tokyo Japan","institution_ids":["https://openalex.org/I4405285273"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011357939","display_name":"Hitomi Sumiyoshi Okuma","orcid":"https://orcid.org/0000-0001-9639-7361","observed_orcids":["https://orcid.org/0000-0001-9639-7361"]},"institutions":[{"id":"https://openalex.org/I4405285273","display_name":"National Cancer Center Hospital","ror":"https://ror.org/053hhpd64","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210087348","https://openalex.org/I4405285273"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hitomi S. Okuma","raw_affiliation_strings":["Clinical Research Support Office National Cancer Center Hospital  Tokyo Japan","Department of Medical Oncology National Cancer Center Hospital  Tokyo Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinical Research Support Office National Cancer Center Hospital  Tokyo Japan","institution_ids":["https://openalex.org/I4405285273"]},{"raw_affiliation_string":"Department of Medical Oncology National Cancer Center Hospital  Tokyo Japan","institution_ids":["https://openalex.org/I4405285273"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033956905","display_name":"Yasushi Goto","orcid":"https://orcid.org/0000-0002-7437-5054","observed_orcids":["https://orcid.org/0000-0002-7437-5054"]},"institutions":[{"id":"https://openalex.org/I4405285273","display_name":"National Cancer Center Hospital","ror":"https://ror.org/053hhpd64","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210087348","https://openalex.org/I4405285273"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yasushi Goto","raw_affiliation_strings":["Department of Thoracic Oncology National Cancer Center Hospital  Tokyo Japan"],"raw_orcid":"https://orcid.org/0000-0002-7437-5054","affiliations":[{"raw_affiliation_string":"Department of Thoracic Oncology National Cancer Center Hospital  Tokyo Japan","institution_ids":["https://openalex.org/I4405285273"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089805529","display_name":"Yoshimasa Saito","orcid":"https://orcid.org/0000-0003-2476-2862","observed_orcids":["https://orcid.org/0000-0003-2476-2862"]},"institutions":[{"id":"https://openalex.org/I4405285273","display_name":"National Cancer Center Hospital","ror":"https://ror.org/053hhpd64","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210087348","https://openalex.org/I4405285273"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yoshimasa Saito","raw_affiliation_strings":["Department of Pharmacy National Cancer Center Hospital  Tokyo Japan"],"raw_orcid":"https://orcid.org/0000-0003-2476-2862","affiliations":[{"raw_affiliation_string":"Department of Pharmacy National Cancer Center Hospital  Tokyo Japan","institution_ids":["https://openalex.org/I4405285273"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100982399","display_name":"Hironobu Hashimoto","orcid":"https://orcid.org/0000-0002-5907-5547","observed_orcids":["https://orcid.org/0000-0002-5907-5547"]},"institutions":[{"id":"https://openalex.org/I4405285273","display_name":"National Cancer Center Hospital","ror":"https://ror.org/053hhpd64","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I4210087348","https://openalex.org/I4405285273"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hironobu Hashimoto","raw_affiliation_strings":["Department of Pharmacy National Cancer Center Hospital  Tokyo Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacy National Cancer Center Hospital  Tokyo Japan","institution_ids":["https://openalex.org/I4405285273"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5087090928"],"corresponding_institution_ids":["https://openalex.org/I4405285273"],"apc_list":{"value":3710,"currency":"USD","value_usd":3710},"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"40","issue":"12","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":0.9229000210762024,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":0.9229000210762024,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12255","display_name":"Biosimilars and Bioanalytical Methods","score":0.0502999983727932,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.0032999999821186066,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alectinib","display_name":"alectinib","score":1.0},{"id":"https://openalex.org/keywords/alk-inhibitors","display_name":"ALK inhibitors","score":0.8420000076293945},{"id":"https://openalex.org/keywords/simultaneous-determination","display_name":"simultaneous determination","score":0.8360000252723694},{"id":"https://openalex.org/keywords/pediatric-pharmacokinetics","display_name":"pediatric pharmacokinetics","score":0.7699999809265137},{"id":"https://openalex.org/keywords/plasma-drug-concentration","display_name":"plasma drug concentration","score":0.42399999499320984},{"id":"https://openalex.org/keywords/lc-ms-ms-method-validation","display_name":"LC-MS/MS method validation","score":0.7200000286102295},{"id":"https://openalex.org/keywords/isotope-labeled-internal-standards","display_name":"isotope-labeled internal standards","score":0.6819999814033508}],"concepts":[{"id":"https://openalex.org/C2779220645","wikidata":"https://www.wikidata.org/wiki/Q21099132","display_name":"Alectinib","level":5,"score":0.9848999977111816},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8169999718666077},{"id":"https://openalex.org/C3020134792","wikidata":"https://www.wikidata.org/wiki/Q79749","display_name":"Human plasma","level":2,"score":0.7346000075340271},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6427000164985657},{"id":"https://openalex.org/C74774607","wikidata":"https://www.wikidata.org/wiki/Q7251495","display_name":"Protein precipitation","level":3,"score":0.548799991607666},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.4569999873638153},{"id":"https://openalex.org/C23531484","wikidata":"https://www.wikidata.org/wiki/Q3196863","display_name":"Calibration curve","level":3,"score":0.45159998536109924},{"id":"https://openalex.org/C117643217","wikidata":"https://www.wikidata.org/wiki/Q17824987","display_name":"Anaplastic lymphoma kinase","level":4,"score":0.42820000648498535},{"id":"https://openalex.org/C149151106","wikidata":"https://www.wikidata.org/wiki/Q4677569","display_name":"Active metabolite","level":3,"score":0.41609999537467957},{"id":"https://openalex.org/C165838908","wikidata":"https://www.wikidata.org/wiki/Q736777","display_name":"Calibration","level":2,"score":0.33009999990463257},{"id":"https://openalex.org/C3020079912","wikidata":"https://www.wikidata.org/wiki/Q3918988","display_name":"Plasma concentration","level":2,"score":0.32670000195503235},{"id":"https://openalex.org/C95986675","wikidata":"https://www.wikidata.org/wiki/Q185168","display_name":"Quantitative analysis (chemistry)","level":2,"score":0.321399986743927},{"id":"https://openalex.org/C112705442","wikidata":"https://www.wikidata.org/wiki/Q323936","display_name":"Pharmacokinetics","level":2,"score":0.30399999022483826},{"id":"https://openalex.org/C3020728648","wikidata":"https://www.wikidata.org/wiki/Q1058795","display_name":"Biological fluids","level":2,"score":0.29600000381469727},{"id":"https://openalex.org/C96618451","wikidata":"https://www.wikidata.org/wiki/Q3028008","display_name":"Dilution","level":2,"score":0.2842999994754791},{"id":"https://openalex.org/C202799725","wikidata":"https://www.wikidata.org/wiki/Q272035","display_name":"Accuracy and precision","level":2,"score":0.266400009393692}],"mesh":[{"descriptor_ui":"D000097922","descriptor_name":"Liquid Chromatography-Mass Spectrometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D002227","descriptor_name":"Carbazoles","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D002227","descriptor_name":"Carbazoles","qualifier_ui":"Q000493","qualifier_name":"pharmacokinetics","is_major_topic":true},{"descriptor_ui":"D002227","descriptor_name":"Carbazoles","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010880","descriptor_name":"Piperidines","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D010880","descriptor_name":"Piperidines","qualifier_ui":"Q000493","qualifier_name":"pharmacokinetics","is_major_topic":true},{"descriptor_ui":"D010880","descriptor_name":"Piperidines","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016014","descriptor_name":"Linear Models","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053719","descriptor_name":"Tandem Mass Spectrometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D057230","descriptor_name":"Limit of Detection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/bmc.70658","doi":"https://doi.org/10.1002/bmc.70658","is_oa":true,"landing_page_url":"https://doi.org/10.1002/bmc.70658","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bmc.70658","source":{"id":"https://openalex.org/S123979729","display_name":"Biomedical Chromatography","issn_l":"0269-3879","issn":["0269-3879","1099-0801"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical Chromatography","raw_type":"journal-article"},{"id":"pmid:43000705","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/43000705","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical chromatography : BMC","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1002/bmc.70658","doi":"https://doi.org/10.1002/bmc.70658","is_oa":true,"landing_page_url":"https://doi.org/10.1002/bmc.70658","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bmc.70658","source":{"id":"https://openalex.org/S123979729","display_name":"Biomedical Chromatography","issn_l":"0269-3879","issn":["0269-3879","1099-0801"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical Chromatography","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9763000011444092,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320311405","display_name":"Japan Agency for Medical Research and Development","ror":"https://ror.org/004rtk039"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225639339.pdf","grobid_xml":"https://content.openalex.org/works/W7225639339.grobid-xml"},"referenced_works_count":19,"referenced_works":["https://openalex.org/W2007894588","https://openalex.org/W2125735484","https://openalex.org/W2345442866","https://openalex.org/W2466940349","https://openalex.org/W2508392847","https://openalex.org/W2515048758","https://openalex.org/W2587216000","https://openalex.org/W2613260339","https://openalex.org/W2624310346","https://openalex.org/W2802668957","https://openalex.org/W2839057355","https://openalex.org/W2885339716","https://openalex.org/W2904760494","https://openalex.org/W2921458278","https://openalex.org/W3042967359","https://openalex.org/W3090473304","https://openalex.org/W3189358425","https://openalex.org/W4389950923","https://openalex.org/W4401549656"],"related_works":["https://openalex.org/W2587216000","https://openalex.org/W4406898957","https://openalex.org/W4214762315","https://openalex.org/W2978387451","https://openalex.org/W2823915827","https://openalex.org/W2537718287","https://openalex.org/W4319864271","https://openalex.org/W7208815394","https://openalex.org/W2902509882","https://openalex.org/W3004869495"],"abstract_inverted_index":{"Alectinib":[0,61],"is":[1,10,25],"an":[2,160],"oral":[3,180],"anaplastic":[4],"lymphoma":[5],"kinase":[6],"inhibitor,":[7],"and":[8,20,46,53,62,74,88,100,115,125,169,192],"M4":[9,21,63,170,193],"its":[11,54],"major":[12,55],"pharmacologically":[13],"active":[14],"metabolite.":[15],"Simultaneous":[16],"quantification":[17,50,97,189],"of":[18,51,69,96,98,164,190],"alectinib":[19,52,87,168,191],"in":[22,58,159,194],"limited-volume":[23,195],"plasma":[24,70,152],"important":[26],"for":[27,48,86,91,132],"pediatric":[28,196],"clinical":[29,162,197],"studies,":[30],"particularly":[31],"when":[32],"concentrations":[33],"are":[34],"low":[35],"or":[36],"exceed":[37],"the":[38,187],"calibration":[39],"range.":[40],"An":[41],"LC-MS/MS":[42,184],"method":[43,147,185],"was":[44,129,148],"developed":[45],"validated":[47],"simultaneous":[49,188],"metabolite,":[56],"M4,":[57,92],"human":[59],"plasma.":[60],"were":[64,83],"extracted":[65],"from":[66,121,155],"20":[67],"\u03bcL":[68],"by":[71],"protein":[72],"precipitation":[73],"quantified":[75],"using":[76],"stable":[77],"isotope-labeled":[78],"internal":[79],"standards.":[80],"Calibration":[81],"ranges":[82],"0.100-20.0":[84],"ng/mL":[85,90],"1.00-200":[89],"with":[93,166],"lower":[94],"limits":[95],"0.100":[99],"1.00":[101],"ng/mL,":[102],"respectively.":[103],"Full":[104],"validation":[105,136],"demonstrated":[106],"acceptable":[107],"selectivity,":[108],"linearity,":[109],"matrix":[110],"effects,":[111],"dilution":[112],"reproducibility,":[113],"stability,":[114],"carryover.":[116],"Inter-day":[117],"accuracy":[118],"(RE)":[119],"ranged":[120],"-9.9%":[122],"to":[123,151,174],"5.1%,":[124],"inter-day":[126],"precision":[127],"(CV)":[128],"\u2264":[130],"8.3%":[131],"both":[133,167],"analytes.":[134],"Partial":[135],"after":[137,177],"analytical":[138],"column":[139],"replacement":[140],"also":[141],"met":[142],"predefined":[143],"acceptance":[144],"criteria.":[145],"The":[146],"successfully":[149],"applied":[150],"samples":[153],"obtained":[154],"one":[156],"patient":[157],"enrolled":[158],"investigator-initiated":[161],"trial":[163],"alectinib,":[165],"remaining":[171],"quantifiable":[172],"up":[173],"72":[175],"h":[176],"a":[178],"single":[179],"dose.":[181],"This":[182],"sensitive":[183],"supports":[186],"study":[198],"samples.":[199]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-10T00:00:00"}
