{"id":"https://openalex.org/W7225628263","doi":"https://doi.org/10.6084/m9.figshare.34952287","title":"Quantification of D55, an apomorphine derivative, in mouse plasma by LC\u2013MS/MS: method development, validation, and improved pharmacokinetics compared with apomorphine","display_name":"Quantification of D55, an apomorphine derivative, in mouse plasma by LC\u2013MS/MS: method development, validation, and improved pharmacokinetics compared with apomorphine","publication_year":2026,"publication_date":"2026-11-09","ids":{"openalex":"https://openalex.org/W7225628263","doi":"https://doi.org/10.6084/m9.figshare.34952287"},"language":"en","primary_location":{"id":"doi:10.6084/m9.figshare.34952287","doi":"https://doi.org/10.6084/m9.figshare.34952287","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.34952287","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.6084/m9.figshare.34952287","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070483268","display_name":"\u5b8f\u5e73 \u6c38\u4e95","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kohei Nagai","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052651256","display_name":"Natsuka Kimura","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Natsuka Kimura","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027739931","display_name":"Tamaki Ishima","orcid":"https://orcid.org/0009-0002-2309-265X","observed_orcids":["https://orcid.org/0009-0002-2309-265X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Tamaki Ishima","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034163966","display_name":"Hitoshi Osaka","orcid":"https://orcid.org/0000-0002-1320-1165","observed_orcids":["https://orcid.org/0000-0002-1320-1165"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hitoshi Osaka","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5122210555","display_name":"Kenichi Aizawa","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kenichi Aizawa","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":0.9613999724388123,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":0.9613999724388123,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10800","display_name":"Forensic Toxicology and Drug Analysis","score":0.02199999988079071,"subfield":{"id":"https://openalex.org/subfields/3005","display_name":"Toxicology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.00430000014603138,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/apomorphine","display_name":"apomorphine","score":0.9860000014305115},{"id":"https://openalex.org/keywords/pharmacokinetics","display_name":"pharmacokinetics","score":0.7900000214576721},{"id":"https://openalex.org/keywords/mitochondrial-disease","display_name":"mitochondrial disease","score":0.7009999752044678},{"id":"https://openalex.org/keywords/plasma-drug-quantitation","display_name":"plasma drug quantification","score":0.6890000104904175},{"id":"https://openalex.org/keywords/lc-ms-ms-method-validation","display_name":"LC-MS/MS method validation","score":0.7910000085830688},{"id":"https://openalex.org/keywords/elimination-half-life","display_name":"elimination half-life","score":0.9049999713897705},{"id":"https://openalex.org/keywords/systemic-exposure","display_name":"systemic exposure","score":0.6830000281333923},{"id":"https://openalex.org/keywords/mouse-plasma","display_name":"mouse plasma","score":0.48500001430511475},{"id":"https://openalex.org/keywords/c57bl-6j-mice","display_name":"C57BL/6J mice","score":0.796999990940094},{"id":"https://openalex.org/keywords/dopamine-receptor-antagonism","display_name":"dopamine receptor antagonism","score":0.382999986410141},{"id":"https://openalex.org/keywords/parkinson-s-disease","display_name":"Parkinson's disease","score":0.48100000619888306},{"id":"https://openalex.org/keywords/non-compartmental-analysis","display_name":"non-compartmental analysis","score":0.6349999904632568}],"concepts":[{"id":"https://openalex.org/C2777525119","wikidata":"https://www.wikidata.org/wiki/Q269111","display_name":"Apomorphine","level":4,"score":0.9208999872207642},{"id":"https://openalex.org/C112705442","wikidata":"https://www.wikidata.org/wiki/Q323936","display_name":"Pharmacokinetics","level":2,"score":0.6521999835968018},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.608299970626831},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.5819000005722046},{"id":"https://openalex.org/C137183658","wikidata":"https://www.wikidata.org/wiki/Q461076","display_name":"Dopaminergic","level":3,"score":0.5353000164031982},{"id":"https://openalex.org/C138944611","wikidata":"https://www.wikidata.org/wiki/Q1163287","display_name":"Clearance","level":2,"score":0.4016000032424927},{"id":"https://openalex.org/C119128265","wikidata":"https://www.wikidata.org/wiki/Q900165","display_name":"Detection limit","level":2,"score":0.3450999855995178},{"id":"https://openalex.org/C2778258207","wikidata":"https://www.wikidata.org/wiki/Q600203","display_name":"Dopamine agonist","level":4,"score":0.33340001106262207},{"id":"https://openalex.org/C2776390293","wikidata":"https://www.wikidata.org/wiki/Q2035485","display_name":"Subcutaneous injection","level":2,"score":0.32249999046325684},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.32109999656677246},{"id":"https://openalex.org/C104488531","wikidata":"https://www.wikidata.org/wiki/Q864212","display_name":"Bioassay","level":2,"score":0.2962000072002411},{"id":"https://openalex.org/C2780035454","wikidata":"https://www.wikidata.org/wiki/Q8386","display_name":"Drug","level":2,"score":0.28139999508857727},{"id":"https://openalex.org/C181389837","wikidata":"https://www.wikidata.org/wiki/Q461809","display_name":"Bioavailability","level":2,"score":0.2705000042915344}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.6084/m9.figshare.34952287","doi":"https://doi.org/10.6084/m9.figshare.34952287","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.34952287","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"},{"id":"doi:10.6084/m9.figshare.34952287.v1","doi":"https://doi.org/10.6084/m9.figshare.34952287.v1","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.34952287.v1","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"},{"id":"doi:10.6084/m9.figshare.34952287.v2","doi":"https://doi.org/10.6084/m9.figshare.34952287.v2","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.34952287.v2","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"}],"best_oa_location":{"id":"doi:10.6084/m9.figshare.34952287","doi":"https://doi.org/10.6084/m9.figshare.34952287","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.34952287","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9763000011444092}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2167242391","https://openalex.org/W3044351026","https://openalex.org/W2385378904","https://openalex.org/W2024982270","https://openalex.org/W2095847332","https://openalex.org/W1975683271","https://openalex.org/W2003660960","https://openalex.org/W1985201145","https://openalex.org/W2028378009","https://openalex.org/W4200161245"],"abstract_inverted_index":{"Apomorphine":[0],"exhibits":[1],"anti-cell":[2,29],"death":[3,30],"activity":[4,16,31],"in":[5,53,214,265],"mitochondrial":[6,188,222,352],"disease":[7],"models,":[8],"but":[9],"its":[10,18,231,238,358,366],"short":[11],"elimination":[12],"half-life":[13,142],"and":[14,32,38,51,56,66,76,96,115,120,132,153,169,230,255,263,288,338,368],"dopaminergic":[15,34],"limit":[17,237],"therapeutic":[19,185],"application.":[20],"D55":[21,50,139,182,240,262,307,326,337,350],"is":[22,241,274],"a":[23,40,87,141,165,172,184,203,242,250,257,298,333],"modified":[24,243],"apomorphine":[25,52,148,246,264,314],"derivative":[26],"with":[27,158,221],"enhanced":[28],"no":[33],"effects.":[35],"We":[36,253,292],"established":[37],"validated":[39],"liquid":[41,276],"chromatography\u2013tandem":[42],"mass":[43],"spectrometry":[44],"method":[45,259,281],"for":[46,187],"simultaneous":[47],"quantification":[48],"of":[49,91,110,147,162,181,217,245,278,286,301,319],"mouse":[54],"plasma":[55,97,269,287],"characterized":[57],"their":[58],"pharmacokinetic":[59],"profiles.":[60],"Validation":[61],"assessed":[62],"selectivity,":[63],"linearity,":[64],"precision":[65,117,129],"accuracy,":[67],"injection":[68,300],"carryover,":[69],"extraction":[70],"recovery,":[71],"matrix":[72],"effects,":[73],"dilution":[74],"integrity,":[75],"stability.":[77],"Male":[78],"C57BL/6J":[79],"mice":[80,297],"(n":[81],"=":[82],"5":[83],"per":[84],"group)":[85],"received":[86],"single":[88,299],"subcutaneous":[89],"dose":[90],"either":[92,302],"compound":[93,303],"(0.5":[94],"mg/kg),":[95],"concentration\u2013time":[98],"data":[99],"were":[100,118],"analyzed":[101],"by":[102],"noncompartmental":[103],"analysis.":[104],"The":[105,280],"assay":[106],"required":[107,282],"10":[108,284],"\u03bcL":[109],"plasma.":[111,322],"For":[112,127],"D55,":[113],"intra-":[114],"inter-day":[116],"\u226410.3%,":[119],"accuracy":[121,133],"ranged":[122,134],"from":[123,135,219,271,321],"86.9%":[124],"to":[125,137,206,260,325,335,340,364],"108.6%.":[126],"apomorphine,":[128],"was":[130,327],"\u22646.8%,":[131],"99.6%":[136],"104.0%.":[138],"exhibited":[140],"3.13-fold":[143],"longer":[144,312],"than":[145,313],"that":[146,195],"(78.4":[149],"vs.":[150],"24.9":[151],"min)":[152],"higher":[154],"systemic":[155],"exposure":[156,324],"(AUC0\u2013\u221e),":[157],"geometric":[159],"mean":[160],"ratios":[161],"2.60":[163],"on":[164,171,233],"mass-concentration":[166],"basis":[167,174],"(ng\u00b7min/mL)":[168],"2.36":[170],"molar-concentration":[173],"(nM\u00b7min).":[175],"These":[176,330],"findings":[177,331],"support":[178],"further":[179,342],"development":[180],"as":[183,249],"candidate":[186],"diseases.":[189],"Mitochondrial":[190],"diseases":[191],"affect":[192],"cell":[193],"structures":[194],"produce":[196],"energy,":[197],"causing":[198],"serious":[199],"health":[200],"problems.":[201],"Apomorphine,":[202],"medicine":[204],"used":[205],"treat":[207],"Parkinson\u2019s":[208],"disease,":[209],"has":[210],"shown":[211],"protective":[212],"effects":[213,232],"laboratory":[215,258],"studies":[216,361],"cells":[218],"patients":[220],"disease.":[223],"However,":[224],"the":[225,275,305,316],"body":[226],"removes":[227],"it":[228,356],"quickly,":[229],"dopamine":[234],"receptors":[235],"may":[236],"use.":[239],"form":[244],"being":[247],"studied":[248],"possible":[251],"treatment.":[252],"developed":[254],"tested":[256],"measure":[261,336],"very":[266],"small":[267],"blood":[268],"samples":[270],"mice.":[272],"Plasma":[273],"part":[277],"blood.":[279],"only":[283],"microliters":[285],"provided":[289],"reliable":[290],"measurements.":[291],"then":[293],"gave":[294],"healthy":[295],"male":[296],"under":[304],"skin.":[306],"persisted":[308],"about":[309],"three":[310],"times":[311],"during":[315],"final":[317],"stage":[318],"clearance":[320],"Overall":[323],"also":[328],"higher.":[329],"provide":[332],"way":[334],"help":[339],"guide":[341],"research.":[343],"This":[344],"study":[345],"did":[346],"not":[347],"test":[348],"whether":[349],"improves":[351],"diseases,":[353],"nor":[354],"does":[355],"determine":[357],"safety.":[359],"Further":[360],"are":[362],"needed":[363],"assess":[365],"benefits":[367],"risks.":[369]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-10T00:00:00"}