{"id":"https://openalex.org/W7225614588","doi":"https://doi.org/10.37275/oaijmr.v6i6.1003","title":"NOS3 (eNOS) mRNA in Whole Blood of Untreated Grade 1 Hypertension: A Case\u2013Control Study with Reference-Gene Sensitivity Analysis","display_name":"NOS3 (eNOS) mRNA in Whole Blood of Untreated Grade 1 Hypertension: A Case\u2013Control Study with Reference-Gene Sensitivity Analysis","publication_year":2026,"publication_date":"2026-11-09","ids":{"openalex":"https://openalex.org/W7225614588","doi":"https://doi.org/10.37275/oaijmr.v6i6.1003"},"language":"en","primary_location":{"id":"doi:10.37275/oaijmr.v6i6.1003","doi":"https://doi.org/10.37275/oaijmr.v6i6.1003","is_oa":true,"landing_page_url":"https://doi.org/10.37275/oaijmr.v6i6.1003","pdf_url":"https://hmpublisher.com/index.php/OAIJMR/article/download/1003/1418","source":{"id":"https://openalex.org/S4210182354","display_name":"Open Access Indonesian Journal of Medical Reviews","issn_l":"2807-6257","issn":["2807-6257"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Open Access Indonesian Journal of Medical Reviews","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://hmpublisher.com/index.php/OAIJMR/article/download/1003/1418","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068245627","display_name":"Diah Mustika","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55892964","display_name":"Andalas University","ror":"https://ror.org/04ded0672","country_code":"ID","type":"education","lineage":["https://openalex.org/I55892964"]}],"countries":["ID"],"is_corresponding":true,"raw_author_name":"Diah Mustika","raw_affiliation_strings":["Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia","institution_ids":["https://openalex.org/I55892964"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5154736011","display_name":"Nur Indrawati Lipoeto","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55892964","display_name":"Andalas University","ror":"https://ror.org/04ded0672","country_code":"ID","type":"education","lineage":["https://openalex.org/I55892964"]}],"countries":["ID"],"is_corresponding":false,"raw_author_name":"Nur Indrawati Lipoeto","raw_affiliation_strings":["Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia","institution_ids":["https://openalex.org/I55892964"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161323749","display_name":"Mohamad Reza","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55892964","display_name":"Andalas University","ror":"https://ror.org/04ded0672","country_code":"ID","type":"education","lineage":["https://openalex.org/I55892964"]}],"countries":["ID"],"is_corresponding":false,"raw_author_name":"Mohamad Reza","raw_affiliation_strings":["Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia","institution_ids":["https://openalex.org/I55892964"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021551555","display_name":"Hirowati Ali","orcid":"https://orcid.org/0000-0002-1720-5147","observed_orcids":["https://orcid.org/0000-0002-1720-5147"]},"institutions":[{"id":"https://openalex.org/I55892964","display_name":"Andalas University","ror":"https://ror.org/04ded0672","country_code":"ID","type":"education","lineage":["https://openalex.org/I55892964"]}],"countries":["ID"],"is_corresponding":false,"raw_author_name":"Hirowati Ali","raw_affiliation_strings":["Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia","institution_ids":["https://openalex.org/I55892964"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003326698","display_name":"Dewi Rusnita","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55892964","display_name":"Andalas University","ror":"https://ror.org/04ded0672","country_code":"ID","type":"education","lineage":["https://openalex.org/I55892964"]}],"countries":["ID"],"is_corresponding":false,"raw_author_name":"Dewi Rusnita","raw_affiliation_strings":["Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia","institution_ids":["https://openalex.org/I55892964"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5161355067","display_name":"Fika Tri Anggraini","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I55892964","display_name":"Andalas University","ror":"https://ror.org/04ded0672","country_code":"ID","type":"education","lineage":["https://openalex.org/I55892964"]}],"countries":["ID"],"is_corresponding":false,"raw_author_name":"Fika Tri Anggraini","raw_affiliation_strings":["Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Master's Program in Biomedical Sciences, Faculty of Medicine, Universitas Andalas, Padang, Indonesia","institution_ids":["https://openalex.org/I55892964"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5068245627"],"corresponding_institution_ids":["https://openalex.org/I55892964"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"6","issue":"6","first_page":"703","last_page":"719"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10144","display_name":"Blood Pressure and Hypertension Studies","score":0.47360000014305115,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10144","display_name":"Blood Pressure and Hypertension Studies","score":0.47360000014305115,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10155","display_name":"Nitric Oxide and Endothelin Effects","score":0.42579999566078186,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.04569999873638153,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[],"concepts":[{"id":"https://openalex.org/C134792803","wikidata":"https://www.wikidata.org/wiki/Q424886","display_name":"Glyceraldehyde 3-phosphate dehydrogenase","level":4,"score":0.8859999775886536},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.6730999946594238},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.6445000171661377},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.5335999727249146},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.4442000091075897},{"id":"https://openalex.org/C2992676626","wikidata":"https://www.wikidata.org/wiki/Q408853","display_name":"Endothelial nitric oxide synthase","level":5,"score":0.4325000047683716},{"id":"https://openalex.org/C84393581","wikidata":"https://www.wikidata.org/wiki/Q82642","display_name":"Blood pressure","level":2,"score":0.3937999904155731},{"id":"https://openalex.org/C2777622882","wikidata":"https://www.wikidata.org/wiki/Q417619","display_name":"Nitric oxide synthase","level":3,"score":0.3937000036239624},{"id":"https://openalex.org/C1621761","wikidata":"https://www.wikidata.org/wiki/Q7997549","display_name":"Whole blood","level":2,"score":0.37279999256134033},{"id":"https://openalex.org/C519581460","wikidata":"https://www.wikidata.org/wiki/Q207843","display_name":"Nitric oxide","level":2,"score":0.36410000920295715},{"id":"https://openalex.org/C67761136","wikidata":"https://www.wikidata.org/wiki/Q2105849","display_name":"Post-hoc analysis","level":2,"score":0.31470000743865967},{"id":"https://openalex.org/C2781197716","wikidata":"https://www.wikidata.org/wiki/Q864574","display_name":"Biomarker","level":2,"score":0.3050000071525574},{"id":"https://openalex.org/C65409693","wikidata":"https://www.wikidata.org/wiki/Q425265","display_name":"Statistical significance","level":2,"score":0.29580000042915344},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.28040000796318054},{"id":"https://openalex.org/C2778096610","wikidata":"https://www.wikidata.org/wiki/Q1476525","display_name":"Dyslipidemia","level":3,"score":0.2793999910354614},{"id":"https://openalex.org/C2985213535","wikidata":"https://www.wikidata.org/wiki/Q1626599","display_name":"Reference values","level":2,"score":0.27549999952316284},{"id":"https://openalex.org/C117220453","wikidata":"https://www.wikidata.org/wiki/Q5172842","display_name":"Correlation","level":2,"score":0.2734000086784363},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.271699994802475},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.25949999690055847},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.25780001282691956}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.37275/oaijmr.v6i6.1003","doi":"https://doi.org/10.37275/oaijmr.v6i6.1003","is_oa":true,"landing_page_url":"https://doi.org/10.37275/oaijmr.v6i6.1003","pdf_url":"https://hmpublisher.com/index.php/OAIJMR/article/download/1003/1418","source":{"id":"https://openalex.org/S4210182354","display_name":"Open Access Indonesian Journal of Medical Reviews","issn_l":"2807-6257","issn":["2807-6257"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Open Access Indonesian Journal of Medical Reviews","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.37275/oaijmr.v6i6.1003","doi":"https://doi.org/10.37275/oaijmr.v6i6.1003","is_oa":true,"landing_page_url":"https://doi.org/10.37275/oaijmr.v6i6.1003","pdf_url":"https://hmpublisher.com/index.php/OAIJMR/article/download/1003/1418","source":{"id":"https://openalex.org/S4210182354","display_name":"Open Access Indonesian Journal of Medical Reviews","issn_l":"2807-6257","issn":["2807-6257"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Open Access Indonesian Journal of Medical Reviews","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225614588.pdf","grobid_xml":"https://content.openalex.org/works/W7225614588.grobid-xml"},"referenced_works_count":79,"referenced_works":["https://openalex.org/W2110748980","https://openalex.org/W3091882950","https://openalex.org/W3109760434","https://openalex.org/W3120368868","https://openalex.org/W3138898349","https://openalex.org/W3168323593","https://openalex.org/W3174986241","https://openalex.org/W3190761114","https://openalex.org/W3193823764","https://openalex.org/W3195069846","https://openalex.org/W3213488383","https://openalex.org/W3216873835","https://openalex.org/W4200051195","https://openalex.org/W4225272803","https://openalex.org/W4229000544","https://openalex.org/W4280516295","https://openalex.org/W4285733775","https://openalex.org/W4286220319","https://openalex.org/W4304080329","https://openalex.org/W4308452875","https://openalex.org/W4310568595","https://openalex.org/W4312113300","https://openalex.org/W4319655952","https://openalex.org/W4323041781","https://openalex.org/W4323843297","https://openalex.org/W4327683219","https://openalex.org/W4360839181","https://openalex.org/W4362634072","https://openalex.org/W4366159532","https://openalex.org/W4368241346","https://openalex.org/W4381598483","https://openalex.org/W4383710599","https://openalex.org/W4386501776","https://openalex.org/W4386827555","https://openalex.org/W4387580075","https://openalex.org/W4387661439","https://openalex.org/W4390203054","https://openalex.org/W4390446464","https://openalex.org/W4390885974","https://openalex.org/W4394980302","https://openalex.org/W4396671342","https://openalex.org/W4396746949","https://openalex.org/W4396946025","https://openalex.org/W4399223136","https://openalex.org/W4399783623","https://openalex.org/W4402664024","https://openalex.org/W4403096839","https://openalex.org/W4404402786","https://openalex.org/W4406668101","https://openalex.org/W4407040317","https://openalex.org/W4408771296","https://openalex.org/W4409732982","https://openalex.org/W4409736934","https://openalex.org/W4410887932","https://openalex.org/W4412451653","https://openalex.org/W4412720533","https://openalex.org/W4413482817","https://openalex.org/W4413800434","https://openalex.org/W4416407440","https://openalex.org/W4416437035","https://openalex.org/W4416542611","https://openalex.org/W7117749907","https://openalex.org/W7127374956","https://openalex.org/W7127969883","https://openalex.org/W7135071053","https://openalex.org/W7154702222","https://openalex.org/W7154731795","https://openalex.org/W7159578141","https://openalex.org/W7161642100","https://openalex.org/W7165053272","https://openalex.org/W7167469301","https://openalex.org/W7167606828","https://openalex.org/W7167941056","https://openalex.org/W7171498715","https://openalex.org/W7171804534","https://openalex.org/W7203840683","https://openalex.org/W7204149834","https://openalex.org/W7214113625","https://openalex.org/W7214207124"],"related_works":[],"abstract_inverted_index":{"Background:":[0],"Reduced":[1],"endothelial":[2],"nitric":[3],"oxide":[4],"synthase":[5],"(eNOS;":[6],"gene":[7],"NOS3)":[8],"activity":[9],"contributes":[10],"to":[11,43,204],"hypertension,":[12],"but":[13],"whether":[14],"NOS3":[15,30,76,149,202,224],"transcripts":[16],"in":[17,20,72,122,164,206,223],"blood":[18],"differ":[19,153],"early,":[21],"untreated":[22,35,57,207],"hypertension":[23,38,62,123,210],"is":[24],"unknown.":[25],"Objective:":[26],"To":[27],"compare":[28],"whole-blood":[29,201],"mRNA":[31],"between":[32],"adults":[33,41,58,66],"with":[34,59,115],"grade":[36,60,208],"1":[37,61,209],"and":[39,42,63,77,100,103,107,193,214],"normotensive":[40,65],"examine":[44],"its":[45],"dependence":[46,93],"on":[47],"the":[48],"reference":[49,233],"gene.":[50],"Methods:":[51],"In":[52],"this":[53],"case\u2013control":[54],"study,":[55],"30":[56,64],"aged":[67],"40\u201355":[68],"years":[69],"were":[70,79,196],"recruited":[71],"West":[73],"Sumatra,":[74],"Indonesia.":[75],"GAPDH":[78,158,205,212,218],"quantified":[80],"by":[81,88,96],"SYBR":[82],"Green":[83],"qRT-PCR;":[84],"\u0394Cq":[85,119],"was":[86,94,110,120,160,169,182,215,227],"compared":[87],"Mann\u2013Whitney":[89],"U":[90],"test.":[91],"Reference-gene":[92],"assessed":[95],"covariance":[97],"adjustment,":[98,219],"slope":[99,168],"efficiency":[101],"analyses":[102],"equivalence":[104],"testing;":[105],"age":[106,192],"sex":[108,194],"adjustment":[109],"bounded":[111],"over":[112],"datasets":[113],"consistent":[114],"group-level":[116],"data.":[117],"Results:":[118],"higher":[121],"(median":[124],"4.05":[125],"vs":[126],"2.18;":[127],"p":[128,142],"=":[129,143,155],"0.046;":[130],"Hodges\u2013Lehmann":[131],"fold":[132,180],"change":[133,181,222],"0.47,":[134],"95%":[135],"CI":[136],"0.18\u20130.99;":[137],"Livak":[138],"0.46,":[139],"0.19\u20131.10;":[140],"Welch":[141],"0.081;":[144],"post":[145],"hoc":[146],"power":[147],"41%).":[148],"Cq":[150,159,213],"did":[151],"not":[152,197,228],"(p":[154,188],"0.383),":[156],"whereas":[157],"1.30":[161],"cycles":[162],"lower":[163],"hypertension.":[165],"The":[166,178],"NOS3\u2013GAPDH":[167],"0.19":[170],"(errors-in-variables":[171],"upper":[172],"bound":[173],"0.58;":[174],"significance":[175],"required":[176],"\u22650.81).":[177],"GAPDH-adjusted":[179],"0.95":[183],"(0.71\u20131.27),":[184],"equivalent":[185],"within":[186],"two-fold":[187],"<":[189],"0.001).":[190],"Adjusted":[191],"effects":[195],"identifiable.":[198],"Conclusion:":[199],"Lower":[200],"relative":[203],"tracked":[211],"absent":[216],"after":[217],"so":[220],"a":[221,231],"transcript":[225],"abundance":[226],"demonstrated.":[229],"With":[230],"single":[232],"gene,":[234],"such":[235],"measurements":[236],"cannot":[237],"be":[238],"interpreted":[239],"without":[240],"multi-reference":[241],"normalization.":[242]},"counts_by_year":[],"updated_date":"2026-11-10T13:44:29.811591","created_date":"2026-11-10T00:00:00"}