{"id":"https://openalex.org/W7225613329","doi":"https://doi.org/10.1038/s41598-026-79345-w","title":"Multicenter evaluation of the EasySeq full-length 16S sequencing kit for bacterial identification in clinical samples","display_name":"Multicenter evaluation of the EasySeq full-length 16S sequencing kit for bacterial identification in clinical samples","publication_year":2026,"publication_date":"2026-11-09","ids":{"openalex":"https://openalex.org/W7225613329","doi":"https://doi.org/10.1038/s41598-026-79345-w"},"language":"en","primary_location":{"id":"doi:10.1038/s41598-026-79345-w","doi":"https://doi.org/10.1038/s41598-026-79345-w","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-026-79345-w","pdf_url":"https://www.nature.com/articles/s41598-026-79345-w_reference.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/s41598-026-79345-w_reference.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059272076","display_name":"Tim T. Severs","orcid":"https://orcid.org/0009-0008-0015-6802","observed_orcids":["https://orcid.org/0009-0008-0015-6802"]},"institutions":[{"id":"https://openalex.org/I1334415907","display_name":"University Medical Center Groningen","ror":"https://ror.org/03cv38k47","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I1334415907"]},{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]},{"id":"https://openalex.org/I4210146510","display_name":"Isala","ror":"https://ror.org/046a2wj10","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210146510"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"T. T. Severs","raw_affiliation_strings":["AGE-Research Group, Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","Laboratory of Medical Microbiology and Infectious Diseases, Isala Academy, Isala Hospital, Zwolle, The Netherlands"],"raw_orcid":"https://orcid.org/0009-0008-0015-6802","affiliations":[{"raw_affiliation_string":"AGE-Research Group, Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I1334415907","https://openalex.org/I169381384"]},{"raw_affiliation_string":"Laboratory of Medical Microbiology and Infectious Diseases, Isala Academy, Isala Hospital, Zwolle, The Netherlands","institution_ids":["https://openalex.org/I4210146510"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161344748","display_name":"A. J. Gebbink","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1334415907","display_name":"University Medical Center Groningen","ror":"https://ror.org/03cv38k47","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I1334415907"]},{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]},{"id":"https://openalex.org/I4210146510","display_name":"Isala","ror":"https://ror.org/046a2wj10","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210146510"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"A. J. Gebbink","raw_affiliation_strings":["AGE-Research Group, Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","Laboratory of Medical Microbiology and Infectious Diseases, Isala Academy, Isala Hospital, Zwolle, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AGE-Research Group, Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I1334415907","https://openalex.org/I169381384"]},{"raw_affiliation_string":"Laboratory of Medical Microbiology and Infectious Diseases, Isala Academy, Isala Hospital, Zwolle, The Netherlands","institution_ids":["https://openalex.org/I4210146510"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161322119","display_name":"L. Gard","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1334415907","display_name":"University Medical Center Groningen","ror":"https://ror.org/03cv38k47","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I1334415907"]},{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"L. Gard","raw_affiliation_strings":["AGE-Research Group, Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AGE-Research Group, Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I1334415907","https://openalex.org/I169381384"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075796846","display_name":"John W. A. Rossen","orcid":"https://orcid.org/0000-0002-7167-8623","observed_orcids":["https://orcid.org/0000-0002-7167-8623"]},"institutions":[{"id":"https://openalex.org/I1334415907","display_name":"University Medical Center Groningen","ror":"https://ror.org/03cv38k47","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I1334415907"]},{"id":"https://openalex.org/I169381384","display_name":"University of Groningen","ror":"https://ror.org/012p63287","country_code":"NL","type":"education","lineage":["https://openalex.org/I169381384"]},{"id":"https://openalex.org/I223532165","display_name":"University of Utah","ror":"https://ror.org/03r0ha626","country_code":"US","type":"education","lineage":["https://openalex.org/I223532165"]},{"id":"https://openalex.org/I4210146510","display_name":"Isala","ror":"https://ror.org/046a2wj10","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210146510"]}],"countries":["NL","US"],"is_corresponding":false,"raw_author_name":"J. W. A. Rossen","raw_affiliation_strings":["AGE-Research Group, Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","Department of Pathology, University of Utah School of Medicine, Salt Lake City, USA","Laboratory of Medical Microbiology and Infectious Diseases, Isala Academy, Isala Hospital, Zwolle, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"AGE-Research Group, Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands","institution_ids":["https://openalex.org/I1334415907","https://openalex.org/I169381384"]},{"raw_affiliation_string":"Department of Pathology, University of Utah School of Medicine, Salt Lake City, USA","institution_ids":["https://openalex.org/I223532165"]},{"raw_affiliation_string":"Laboratory of Medical Microbiology and Infectious Diseases, Isala Academy, Isala Hospital, Zwolle, The Netherlands","institution_ids":["https://openalex.org/I4210146510"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091466004","display_name":"Lieke B. van Alphen","orcid":"https://orcid.org/0000-0001-9541-7172","observed_orcids":["https://orcid.org/0000-0001-9541-7172"]},"institutions":[{"id":"https://openalex.org/I2800191616","display_name":"Maastricht University Medical Centre","ror":"https://ror.org/02d9ce178","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I2800191616"]},{"id":"https://openalex.org/I34352273","display_name":"Maastricht University","ror":"https://ror.org/02jz4aj89","country_code":"NL","type":"education","lineage":["https://openalex.org/I34352273"]},{"id":"https://openalex.org/I4405268545","display_name":"Care and Public Health Research Institute","ror":"https://ror.org/01930wn69","country_code":"NL","type":"facility","lineage":["https://openalex.org/I34352273","https://openalex.org/I4405268545"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"L. B. van Alphen","raw_affiliation_strings":["Department of Medical Microbiology, Infectious Diseases and Infection Prevention, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Center+ (MUMC+), Maastricht, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Microbiology, Infectious Diseases and Infection Prevention, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Center+ (MUMC+), Maastricht, The Netherlands","institution_ids":["https://openalex.org/I2800191616","https://openalex.org/I34352273","https://openalex.org/I4405268545"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161349253","display_name":"R. M. Koeck","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2800191616","display_name":"Maastricht University Medical Centre","ror":"https://ror.org/02d9ce178","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I2800191616"]},{"id":"https://openalex.org/I34352273","display_name":"Maastricht University","ror":"https://ror.org/02jz4aj89","country_code":"NL","type":"education","lineage":["https://openalex.org/I34352273"]},{"id":"https://openalex.org/I4405268545","display_name":"Care and Public Health Research Institute","ror":"https://ror.org/01930wn69","country_code":"NL","type":"facility","lineage":["https://openalex.org/I34352273","https://openalex.org/I4405268545"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"R. M. Koeck","raw_affiliation_strings":["Department of Medical Microbiology, Infectious Diseases and Infection Prevention, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Center+ (MUMC+), Maastricht, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Microbiology, Infectious Diseases and Infection Prevention, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Center+ (MUMC+), Maastricht, The Netherlands","institution_ids":["https://openalex.org/I2800191616","https://openalex.org/I34352273","https://openalex.org/I4405268545"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073720319","display_name":"Pieter Willem Smit","orcid":"https://orcid.org/0000-0003-1053-3591","observed_orcids":["https://orcid.org/0000-0003-1053-3591"]},"institutions":[{"id":"https://openalex.org/I4210108903","display_name":"Maasstad Ziekenhuis","ror":"https://ror.org/01n0rnc91","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210108903"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"P. W. Smit","raw_affiliation_strings":["Department of Medical Microbiology, Laboratory, Molecular Diagnostics Unit, Maasstad Hospital, Rotterdam, The Netherlands"],"raw_orcid":"https://orcid.org/0000-0003-1053-3591","affiliations":[{"raw_affiliation_string":"Department of Medical Microbiology, Laboratory, Molecular Diagnostics Unit, Maasstad Hospital, Rotterdam, The Netherlands","institution_ids":["https://openalex.org/I4210108903"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008946533","display_name":"Rachid Y Yahiaoui","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210108903","display_name":"Maasstad Ziekenhuis","ror":"https://ror.org/01n0rnc91","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I4210108903"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"R. Y. Yahiaoui","raw_affiliation_strings":["Department of Medical Microbiology, Laboratory, Molecular Diagnostics Unit, Maasstad Hospital, Rotterdam, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Microbiology, Laboratory, Molecular Diagnostics Unit, Maasstad Hospital, Rotterdam, The Netherlands","institution_ids":["https://openalex.org/I4210108903"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083944912","display_name":"S\u00e9bastien Matamoros","orcid":"https://orcid.org/0000-0001-6132-2298","observed_orcids":["https://orcid.org/0000-0001-6132-2298"]},"institutions":[{"id":"https://openalex.org/I2802928900","display_name":"Amsterdam UMC Location University of Amsterdam","ror":"https://ror.org/03t4gr691","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I2802928900","https://openalex.org/I4210151833"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"S. P. F. Matamoros","raw_affiliation_strings":["Medical Microbiology and Infection Control, Amsterdam UMC Location University of Amsterdam, Amsterdam, The Netherlands"],"raw_orcid":"https://orcid.org/0000-0001-6132-2298","affiliations":[{"raw_affiliation_string":"Medical Microbiology and Infection Control, Amsterdam UMC Location University of Amsterdam, Amsterdam, The Netherlands","institution_ids":["https://openalex.org/I2802928900"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161333857","display_name":"B. van den Bosch","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2802934949","display_name":"Radboud University Medical Center","ror":"https://ror.org/05wg1m734","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I2802934949"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"B. van den Bosch","raw_affiliation_strings":["Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands","institution_ids":["https://openalex.org/I2802934949"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161354851","display_name":"P. Koopman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2802934949","display_name":"Radboud University Medical Center","ror":"https://ror.org/05wg1m734","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I2802934949"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"P. Koopman","raw_affiliation_strings":["Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands","institution_ids":["https://openalex.org/I2802934949"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013727923","display_name":"R. Cartuyvels","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210095612","display_name":"Jessa Hospital","ror":"https://ror.org/00qkhxq50","country_code":"BE","type":"healthcare","lineage":["https://openalex.org/I4210095612"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"R. Cartuyvels","raw_affiliation_strings":["Laboratory for Molecular Diagnostics, Department of Clinical Biology, Jessa Ziekenhuis, Hasselt, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory for Molecular Diagnostics, Department of Clinical Biology, Jessa Ziekenhuis, Hasselt, Belgium","institution_ids":["https://openalex.org/I4210095612"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037338794","display_name":"Jozef Dingemans","orcid":"https://orcid.org/0000-0001-8079-3087","observed_orcids":["https://orcid.org/0000-0001-8079-3087"]},"institutions":[{"id":"https://openalex.org/I4210095612","display_name":"Jessa Hospital","ror":"https://ror.org/00qkhxq50","country_code":"BE","type":"healthcare","lineage":["https://openalex.org/I4210095612"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"J. Dingemans","raw_affiliation_strings":["Laboratory for Molecular Diagnostics, Department of Clinical Biology, Jessa Ziekenhuis, Hasselt, Belgium"],"raw_orcid":"https://orcid.org/0000-0001-8079-3087","affiliations":[{"raw_affiliation_string":"Laboratory for Molecular Diagnostics, Department of Clinical Biology, Jessa Ziekenhuis, Hasselt, Belgium","institution_ids":["https://openalex.org/I4210095612"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161345554","display_name":"M. J. R. Ivens","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I2800006345"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"M. J. R. Ivens","raw_affiliation_strings":["Center for Infectious Diseases, Medical Microbiology and Infection Control, Leiden University Medical Center, Leiden, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Infectious Diseases, Medical Microbiology and Infection Control, Leiden University Medical Center, Leiden, The Netherlands","institution_ids":["https://openalex.org/I2800006345"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024615976","display_name":"Suzan D. Pas","orcid":"https://orcid.org/0000-0003-1943-1217","observed_orcids":["https://orcid.org/0000-0003-1943-1217"]},"institutions":[{"id":"https://openalex.org/I2802934949","display_name":"Radboud University Medical Center","ror":"https://ror.org/05wg1m734","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I2802934949"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"S. D. Pas","raw_affiliation_strings":["Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands"],"raw_orcid":"https://orcid.org/0000-0003-1943-1217","affiliations":[{"raw_affiliation_string":"Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands","institution_ids":["https://openalex.org/I2802934949"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025436708","display_name":"Stefan A. Boers","orcid":"https://orcid.org/0000-0002-1560-6799","observed_orcids":["https://orcid.org/0000-0002-1560-6799"]},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"healthcare","lineage":["https://openalex.org/I2800006345"]}],"countries":["NL"],"is_corresponding":true,"raw_author_name":"S. A. Boers","raw_affiliation_strings":["Center for Infectious Diseases, Medical Microbiology and Infection Control, Leiden University Medical Center, Leiden, The Netherlands"],"raw_orcid":"https://orcid.org/0000-0002-1560-6799","affiliations":[{"raw_affiliation_string":"Center for Infectious Diseases, Medical Microbiology and Infection Control, Leiden University Medical Center, Leiden, The Netherlands","institution_ids":["https://openalex.org/I2800006345"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":12,"corresponding_author_ids":["https://openalex.org/A5025436708"],"corresponding_institution_ids":["https://openalex.org/I2800006345"],"apc_list":{"value":2690,"currency":"USD","value_usd":2690},"apc_paid":{"value":2690,"currency":"USD","value_usd":2690},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.0032999999821186066,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/16s-rrna-gene-sequencing","display_name":"16S rRNA gene sequencing","score":0.8619999885559082},{"id":"https://openalex.org/keywords/bacterial-identification","display_name":"bacterial identification","score":0.8539999723434448},{"id":"https://openalex.org/keywords/clinical-microbiology","display_name":"clinical microbiology","score":0.7649999856948853},{"id":"https://openalex.org/keywords/oxford-nanopore-sequencing","display_name":"Oxford Nanopore sequencing","score":0.7990000247955322},{"id":"https://openalex.org/keywords/interlaboratory-reproducibility","display_name":"interlaboratory reproducibility","score":0.8790000081062317},{"id":"https://openalex.org/keywords/mock-communities","display_name":"mock communities","score":0.7480000257492065}],"concepts":[{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.5074999928474426},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.48069998621940613},{"id":"https://openalex.org/C149453125","wikidata":"https://www.wikidata.org/wiki/Q5437094","display_name":"Fastidious organism","level":3,"score":0.46219998598098755},{"id":"https://openalex.org/C67905577","wikidata":"https://www.wikidata.org/wiki/Q215980","display_name":"Ribosomal RNA","level":3,"score":0.4140999913215637},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4034999907016754},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.33480000495910645},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3190000057220459},{"id":"https://openalex.org/C126513998","wikidata":"https://www.wikidata.org/wiki/Q8973627","display_name":"Nanopore sequencing","level":4,"score":0.3125},{"id":"https://openalex.org/C2992435398","wikidata":"https://www.wikidata.org/wiki/Q6934595","display_name":"Multicenter study","level":3,"score":0.30000001192092896},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.28630000352859497},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.28619998693466187},{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.2858999967575073},{"id":"https://openalex.org/C2993206177","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon sequencing","level":4,"score":0.2815999984741211},{"id":"https://openalex.org/C22860579","wikidata":"https://www.wikidata.org/wiki/Q7322402","display_name":"Ribosomal Intergenic Spacer analysis","level":5,"score":0.28029999136924744},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.27799999713897705},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.26579999923706055},{"id":"https://openalex.org/C195139083","wikidata":"https://www.wikidata.org/wiki/Q3463539","display_name":"Intergenic region","level":4,"score":0.2554999887943268},{"id":"https://openalex.org/C189539697","wikidata":"https://www.wikidata.org/wiki/Q1125312","display_name":"Microbiological culture","level":3,"score":0.2513999938964844}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1038/s41598-026-79345-w","doi":"https://doi.org/10.1038/s41598-026-79345-w","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-026-79345-w","pdf_url":"https://www.nature.com/articles/s41598-026-79345-w_reference.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1038/s41598-026-79345-w","doi":"https://doi.org/10.1038/s41598-026-79345-w","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-026-79345-w","pdf_url":"https://www.nature.com/articles/s41598-026-79345-w_reference.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8371999859809875}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225613329.pdf","grobid_xml":"https://content.openalex.org/works/W7225613329.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4417157339","https://openalex.org/W2624010287","https://openalex.org/W7171803120","https://openalex.org/W4409669136","https://openalex.org/W2519453043","https://openalex.org/W3123878558","https://openalex.org/W4414082665","https://openalex.org/W3212770737","https://openalex.org/W2996962598","https://openalex.org/W4412991533"],"abstract_inverted_index":{"Bacterial":[0],"culture":[1],"remains":[2],"essential":[3],"for":[4,54],"pathogen":[5],"detection":[6],"and":[7,35,72,85,94,137,168],"identification,":[8],"but":[9],"its":[10],"sensitivity":[11],"is":[12,36],"limited":[13],"in":[14,40,82,92,162,166,185],"cases":[15],"involving":[16],"fastidious":[17],"microorganisms,":[18],"polymicrobial":[19],"infections,":[20],"or":[21],"samples":[22,97,187,191],"obtained":[23],"after":[24],"antibiotic":[25],"treatment.":[26],"Targeted":[27],"next-generation":[28],"sequencing":[29],"(tNGS)":[30],"offers":[31],"a":[32,88],"culture-independent":[33],"alternative":[34],"increasingly":[37],"being":[38],"implemented":[39],"clinical":[41,96,119],"microbiology.":[42],"This":[43],"multicenter":[44],"study":[45],"evaluated":[46],"the":[47,62,83,132],"EasySeq":[48],"Full-Length":[49],"16S":[50,69],"Library":[51],"Prep":[52],"Kit":[53],"Oxford":[55],"Nanopore":[56],"Technologies":[57],"(EasySeq;":[58],"NimaGen)":[59],"combined":[60],"with":[61,113,182],"GenomeDetective":[63],"pipeline":[64],"(EasySeq-GD)":[65],"against":[66],"eight":[67,167],"established":[68],"rRNA":[70,74],"gene-":[71],"16\u201323\u200bS":[73],"intergenic":[75],"spacer":[76],"(IS)-based":[77],"workflows.":[78],"Eight":[79],"medical":[80],"centers":[81,112],"Netherlands":[84],"Belgium":[86],"analyzed":[87],"mock":[89],"community":[90,105],"sample":[91],"triplicate":[93],"20":[95],"per":[98],"center":[99],"(":[100],"n":[101],"=":[102],"160).":[103],"Mock":[104],"analysis":[106],"demonstrated":[107,174],"consistent":[108],"taxonomic":[109],"profiles":[110],"across":[111],"minimal":[114],"intralaboratory":[115],"variation.":[116],"Of":[117],"160":[118],"samples,":[120,135,170],"19":[121],"(11.9%)":[122],"initially":[123],"failed,":[124],"of":[125],"which":[126],"nine":[127],"(5.6%)":[128],"remained":[129],"unresolved.":[130],"Among":[131],"remaining":[133],"151":[134],"EasySeq-GD":[136,146,173],"comparator":[138,153,183],"workflows":[139,154,184],"shared":[140],"141":[141],"detected":[142],"taxa.":[143,158],"In":[144],"addition,":[145],"identified":[147,155],"92":[148],"unique":[149,157],"bacterial":[150],"taxa,":[151],"whereas":[152],"32":[156],"These":[159],"findings":[160],"resulted":[161],"clinically":[163,195],"relevant":[164,196],"discrepancies":[165],"three":[169],"respectively.":[171],"Overall,":[172],"good":[175],"interlaboratory":[176],"reproducibility.":[177],"Taxon-level":[178],"results":[179],"were":[180],"concordant":[181],"107/151":[186],"(70.9%),":[188],"while":[189],"140/151":[190],"(92.7%)":[192],"showed":[193],"no":[194],"discrepancies.":[197]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-10T00:00:00"}