{"id":"https://openalex.org/W7225612905","doi":"https://doi.org/10.3389/fpls.2026.1997129","title":"Functional characterization of multiple terpene synthases from Idesia polycarpa reveals diverse catalytic activities in vitro and in planta","display_name":"Functional characterization of multiple terpene synthases from Idesia polycarpa reveals diverse catalytic activities in vitro and in planta","publication_year":2026,"publication_date":"2026-11-09","ids":{"openalex":"https://openalex.org/W7225612905","doi":"https://doi.org/10.3389/fpls.2026.1997129"},"language":"en","primary_location":{"id":"doi:10.3389/fpls.2026.1997129","doi":"https://doi.org/10.3389/fpls.2026.1997129","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2026.1997129","pdf_url":"https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2026.1997129/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2026.1997129/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5128262986","display_name":"Stina Strassburg","orcid":"https://orcid.org/0009-0009-9965-6678","observed_orcids":["https://orcid.org/0009-0009-9965-6678"]},"institutions":[{"id":"https://openalex.org/I25974101","display_name":"University of W\u00fcrzburg","ror":"https://ror.org/00fbnyb24","country_code":"DE","type":"education","lineage":["https://openalex.org/I25974101"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Stina Strassburg","raw_affiliation_strings":["Julius-Maximilians-University W\u00fcrzburg, Julius-von-Sachs-Institute for Biosciences, Chair of Pharmaceutical Biology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Julius-Maximilians-University W\u00fcrzburg, Julius-von-Sachs-Institute for Biosciences, Chair of Pharmaceutical Biology","institution_ids":["https://openalex.org/I25974101"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5128257070","display_name":"Melina Panagoulias","orcid":"https://orcid.org/0009-0006-8339-4018","observed_orcids":["https://orcid.org/0009-0006-8339-4018"]},"institutions":[{"id":"https://openalex.org/I25974101","display_name":"University of W\u00fcrzburg","ror":"https://ror.org/00fbnyb24","country_code":"DE","type":"education","lineage":["https://openalex.org/I25974101"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Melina Panagoulias","raw_affiliation_strings":["Julius-Maximilians-University W\u00fcrzburg, Julius-von-Sachs-Institute for Biosciences, Chair of Pharmaceutical Biology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Julius-Maximilians-University W\u00fcrzburg, Julius-von-Sachs-Institute for Biosciences, Chair of Pharmaceutical Biology","institution_ids":["https://openalex.org/I25974101"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5054825705","display_name":"Nathalie D. Lackus","orcid":"https://orcid.org/0000-0002-0419-8937","observed_orcids":["https://orcid.org/0000-0002-0419-8937"]},"institutions":[{"id":"https://openalex.org/I25974101","display_name":"University of W\u00fcrzburg","ror":"https://ror.org/00fbnyb24","country_code":"DE","type":"education","lineage":["https://openalex.org/I25974101"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Nathalie D. Lackus","raw_affiliation_strings":["Julius-Maximilians-University W\u00fcrzburg, Julius-von-Sachs-Institute for Biosciences, Chair of Pharmaceutical Biology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Julius-Maximilians-University W\u00fcrzburg, Julius-von-Sachs-Institute for Biosciences, Chair of Pharmaceutical Biology","institution_ids":["https://openalex.org/I25974101"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5054825705"],"corresponding_institution_ids":["https://openalex.org/I25974101"],"apc_list":{"value":3150,"currency":"CHF","value_usd":3801},"apc_paid":{"value":3150,"currency":"CHF","value_usd":3801},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"17","issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10135","display_name":"Insect-Plant Interactions and Control","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1109","display_name":"Insect Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/terpene-synthase","display_name":"terpene synthase","score":0.9959999918937683},{"id":"https://openalex.org/keywords/idesia-polycarpa","display_name":"Idesia polycarpa","score":0.996999979019165},{"id":"https://openalex.org/keywords/herbivore-induced-plant-volatiles","display_name":"herbivore-induced plant volatiles","score":0.9700000286102295},{"id":"https://openalex.org/keywords/leaf-volatiles","display_name":"leaf volatiles","score":0.7070000171661377},{"id":"https://openalex.org/keywords/monoterpenes","display_name":"monoterpenes","score":0.8389999866485596},{"id":"https://openalex.org/keywords/sesquiterpenes","display_name":"sesquiterpenes","score":1.0},{"id":"https://openalex.org/keywords/e-beta-ocimene","display_name":"(E)-\u03b2-ocimene","score":0.906000018119812},{"id":"https://openalex.org/keywords/1-8-cineole","display_name":"1,8-cineole","score":1.0},{"id":"https://openalex.org/keywords/germacrene-d","display_name":"germacrene D","score":0.996999979019165},{"id":"https://openalex.org/keywords/e-e-alpha-farnesene","display_name":"(E,E)-\u03b1-farnesene","score":0.9670000076293945},{"id":"https://openalex.org/keywords/plant-defense","display_name":"plant defense","score":0.7760000228881836},{"id":"https://openalex.org/keywords/salicaceae","display_name":"Salicaceae","score":0.8080000281333923},{"id":"https://openalex.org/keywords/terpene-biosynthesis","display_name":"terpene biosynthesis","score":0.7570000290870667}],"concepts":[{"id":"https://openalex.org/C44937612","wikidata":"https://www.wikidata.org/wiki/Q212364","display_name":"Terpene","level":2,"score":0.8212000131607056},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6362000107765198},{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.5684000253677368},{"id":"https://openalex.org/C2778182457","wikidata":"https://www.wikidata.org/wiki/Q5550970","display_name":"Germacrene","level":3,"score":0.4936999976634979},{"id":"https://openalex.org/C2777671706","wikidata":"https://www.wikidata.org/wiki/Q32528","display_name":"Monoterpene","level":2,"score":0.47859999537467957},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.47380000352859497},{"id":"https://openalex.org/C2776211264","wikidata":"https://www.wikidata.org/wiki/Q80218","display_name":"Sesquiterpene","level":2,"score":0.47110000252723694},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.46700000762939453},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.45570001006126404},{"id":"https://openalex.org/C2780562374","wikidata":"https://www.wikidata.org/wiki/Q3024956","display_name":"Nicotiana benthamiana","level":3,"score":0.4350999891757965},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4284000098705292},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.40939998626708984},{"id":"https://openalex.org/C183570298","wikidata":"https://www.wikidata.org/wiki/Q2004252","display_name":"Plant defense against herbivory","level":3,"score":0.3707999885082245},{"id":"https://openalex.org/C2776055928","wikidata":"https://www.wikidata.org/wiki/Q700895","display_name":"Green leaf volatiles","level":3,"score":0.3328000009059906},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.3312000036239624},{"id":"https://openalex.org/C206103511","wikidata":"https://www.wikidata.org/wiki/Q426694","display_name":"Terpenoid","level":2,"score":0.32280001044273376},{"id":"https://openalex.org/C2780250286","wikidata":"https://www.wikidata.org/wiki/Q149382","display_name":"Populus trichocarpa","level":4,"score":0.3003000020980835},{"id":"https://openalex.org/C2777942875","wikidata":"https://www.wikidata.org/wiki/Q26840883","display_name":"Methyl jasmonate","level":3,"score":0.29260000586509705},{"id":"https://openalex.org/C5911399","wikidata":"https://www.wikidata.org/wiki/Q2278983","display_name":"Gene family","level":4,"score":0.28870001435279846},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2773999869823456},{"id":"https://openalex.org/C2779906576","wikidata":"https://www.wikidata.org/wiki/Q2095629","display_name":"Pinene","level":2,"score":0.26269999146461487},{"id":"https://openalex.org/C2781205006","wikidata":"https://www.wikidata.org/wiki/Q181095","display_name":"Nicotiana tabacum","level":3,"score":0.2605000138282776},{"id":"https://openalex.org/C59920403","wikidata":"https://www.wikidata.org/wiki/Q698044","display_name":"Aroma of wine","level":3,"score":0.25679999589920044}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3389/fpls.2026.1997129","doi":"https://doi.org/10.3389/fpls.2026.1997129","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2026.1997129","pdf_url":"https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2026.1997129/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.3389/fpls.2026.1997129","doi":"https://doi.org/10.3389/fpls.2026.1997129","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fpls.2026.1997129","pdf_url":"https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2026.1997129/pdf","source":{"id":"https://openalex.org/S2493613807","display_name":"Frontiers in Plant Science","issn_l":"1664-462X","issn":["1664-462X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Plant Science","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320325356","display_name":"Julius-Maximilians-Universit\u00e4t W\u00fcrzburg","ror":"https://ror.org/00fbnyb24"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225612905.pdf","grobid_xml":"https://content.openalex.org/works/W7225612905.grobid-xml"},"referenced_works_count":37,"referenced_works":["https://openalex.org/W1487781539","https://openalex.org/W1771477172","https://openalex.org/W1904809696","https://openalex.org/W1928030931","https://openalex.org/W1980346224","https://openalex.org/W1987927621","https://openalex.org/W2019159946","https://openalex.org/W2025767103","https://openalex.org/W2043165163","https://openalex.org/W2084082320","https://openalex.org/W2103353162","https://openalex.org/W2107229549","https://openalex.org/W2108835954","https://openalex.org/W2109365702","https://openalex.org/W2123912338","https://openalex.org/W2125360702","https://openalex.org/W2132805244","https://openalex.org/W2146494718","https://openalex.org/W2149430024","https://openalex.org/W2152850307","https://openalex.org/W2157843402","https://openalex.org/W2170278083","https://openalex.org/W2606346301","https://openalex.org/W2750432728","https://openalex.org/W2900956676","https://openalex.org/W2904574294","https://openalex.org/W2915548729","https://openalex.org/W2945188084","https://openalex.org/W2953426023","https://openalex.org/W2971082441","https://openalex.org/W2977368063","https://openalex.org/W3121944103","https://openalex.org/W4251751280","https://openalex.org/W4309738995","https://openalex.org/W4399802798","https://openalex.org/W7134065931","https://openalex.org/W7203827215"],"related_works":["https://openalex.org/W7134065931","https://openalex.org/W2107229549","https://openalex.org/W2953426023","https://openalex.org/W2019159946","https://openalex.org/W2765689427","https://openalex.org/W7203827215","https://openalex.org/W7203886551","https://openalex.org/W3205988618","https://openalex.org/W4402883685","https://openalex.org/W2904574294"],"abstract_inverted_index":{"Introduction":[0],"Terpenes":[1],"are":[2,16],"an":[3],"important":[4],"and":[5,14,129,174,183,233],"structurally":[6],"diverse":[7],"class":[8],"of":[9,19,29,48,60,81,87,141,147,196,238],"specialized":[10],"metabolites.":[11],"Volatile":[12],"mono-":[13],"sesquiterpenes":[15,179],"often":[17],"components":[18],"herbivore-induced":[20,62,88,225],"volatile":[21,49,95,207,226],"bouquets":[22],"in":[23,64,84,90,120,155,160,166,201,212,228,242],"perennial":[24],"plants,":[25],"including":[26,123,180],"tree":[27],"species":[28,232],"the":[30,35,52,58,79,85,91,142,148,197,221,235],"Salicaceae":[31],"family":[32],"such":[33,169],"as":[34,136,170,176,178],"Japanese":[36],"orange":[37],"cherry":[38],"(":[39,171,184],"Idesia":[40],"polycarpa":[41,66,93,106],"),":[42],"which":[43],"produces":[44],"a":[45,99,229],"wide":[46],"array":[47],"terpenes.":[50],"However,":[51],"terpene":[53,191],"synthases":[54],"(TPSs)":[55],"contributing":[56],"to":[57,77,157],"formation":[59,86],"these":[61],"volatiles":[63],"I.":[65,92,105],"have":[67],"not":[68],"yet":[69],"been":[70],"characterized.":[71],"In":[72],"this":[73],"study,":[74],"we":[75,109],"aimed":[76],"characterize":[78],"complement":[80],"TPSs":[82],"involved":[83],"terpenes":[89],"leaf":[94,107],"bouquet.":[96],"Methods":[97],"Using":[98],"previously":[100],"generated":[101],"de":[102],"novo":[103],"assembled":[104],"transcriptome,":[108],"identified":[110,149,198],"seven":[111],"candidate":[112],"TPS":[113,137,150,240],"genes":[114,151,200],"through":[115],"comprehensive":[116],"tBLASTn":[117],"analysis.":[118],"Subsequent":[119],"silico":[121],"analyses,":[122],"phylogenetic":[124],"analysis,":[125,208],"subcellular":[126],"localization":[127],"prediction,":[128],"conserved":[130],"motif":[131],"identification,":[132],"supported":[133],"their":[134,164,210],"assignment":[135],"candidates.":[138],"Results":[139],"Reanalysis":[140],"transcriptome":[143],"revealed":[144],"that":[145],"six":[146],"were":[152],"significantly":[153],"upregulated":[154],"response":[156],"herbivory.":[158],"Functional":[159],"vitro":[161],"characterization":[162],"confirmed":[163],"activity":[165,211,237],"producing":[167],"monoterpenes":[168],"E":[172,185,187],")-\u03b2-ocimene":[173],"1,8-cineole,":[175],"well":[177],"germacrene":[181],"D":[182],",":[186,204],")-\u03b1-farnesene,":[188],"among":[189],"other":[190],"compounds.":[192],"Furthermore,":[193],"heterologous":[194],"overexpression":[195],"IpTPS":[199],"Nicotiana":[202],"benthamiana":[203],"followed":[205],"by":[206],"validated":[209],"planta":[213],".":[214],"Discussion":[215],"These":[216],"findings":[217],"provide":[218],"insights":[219],"into":[220],"TPS-mediated":[222],"biosynthesis":[223],"underlying":[224],"emissions":[227],"non-model":[230],"woody":[231],"highlight":[234],"coordinated":[236],"multiple":[239],"enzymes":[241],"plant":[243],"defense.":[244]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-10T00:00:00"}