{"id":"https://openalex.org/W7225450397","doi":"https://doi.org/10.1186/s12934-026-03167-8","title":"Adaptive laboratory evolution of Cupriavidus necator to sodium salts as a whole cell engineering strategy for enhanced trehalose production","display_name":"Adaptive laboratory evolution of Cupriavidus necator to sodium salts as a whole cell engineering strategy for enhanced trehalose production","publication_year":2026,"publication_date":"2026-11-07","ids":{"openalex":"https://openalex.org/W7225450397","doi":"https://doi.org/10.1186/s12934-026-03167-8"},"language":"en","primary_location":{"id":"doi:10.1186/s12934-026-03167-8","doi":"https://doi.org/10.1186/s12934-026-03167-8","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12934-026-03167-8","pdf_url":null,"source":{"id":"https://openalex.org/S171368329","display_name":"Microbial Cell Factories","issn_l":"1475-2859","issn":["1475-2859"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbial Cell Factories","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","datacite","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1186/s12934-026-03167-8","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028633507","display_name":"Lisa van der Sande","orcid":"https://orcid.org/0000-0003-0048-1169","observed_orcids":["https://orcid.org/0000-0003-0048-1169"]},"institutions":[{"id":"https://openalex.org/I102335020","display_name":"Karlsruhe Institute of Technology","ror":"https://ror.org/04t3en479","country_code":"DE","type":"education","lineage":["https://openalex.org/I102335020","https://openalex.org/I1305996414"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Lisa van der Sande","raw_affiliation_strings":["Process Engineering in Life Science, Electrobiotechnology, Karlsruhe Institute of Technology, Karlsruhe, 76131, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Process Engineering in Life Science, Electrobiotechnology, Karlsruhe Institute of Technology, Karlsruhe, 76131, Germany","institution_ids":["https://openalex.org/I102335020"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5151628139","display_name":"Manuel M\u00fcller","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I102335020","display_name":"Karlsruhe Institute of Technology","ror":"https://ror.org/04t3en479","country_code":"DE","type":"education","lineage":["https://openalex.org/I102335020","https://openalex.org/I1305996414"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Manuel M\u00fcller","raw_affiliation_strings":["Process Engineering in Life Science, Electrobiotechnology, Karlsruhe Institute of Technology, Karlsruhe, 76131, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Process Engineering in Life Science, Electrobiotechnology, Karlsruhe Institute of Technology, Karlsruhe, 76131, Germany","institution_ids":["https://openalex.org/I102335020"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037948653","display_name":"Habibu Aliyu","orcid":"https://orcid.org/0000-0002-9870-8167","observed_orcids":["https://orcid.org/0000-0002-9870-8167"]},"institutions":[{"id":"https://openalex.org/I102335020","display_name":"Karlsruhe Institute of Technology","ror":"https://ror.org/04t3en479","country_code":"DE","type":"education","lineage":["https://openalex.org/I102335020","https://openalex.org/I1305996414"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Habibu Aliyu","raw_affiliation_strings":["Institute for Biological Interfaces 5, Microbial Genetics & Biotechnology, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, 76344, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Biological Interfaces 5, Microbial Genetics & Biotechnology, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, 76344, Germany","institution_ids":["https://openalex.org/I102335020"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036048756","display_name":"Anne\u2010Kristin Kaster","orcid":"https://orcid.org/0000-0002-6361-9551","observed_orcids":["https://orcid.org/0000-0002-6361-9551"]},"institutions":[{"id":"https://openalex.org/I102335020","display_name":"Karlsruhe Institute of Technology","ror":"https://ror.org/04t3en479","country_code":"DE","type":"education","lineage":["https://openalex.org/I102335020","https://openalex.org/I1305996414"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Anne-Kristin Kaster","raw_affiliation_strings":["Institute for Biological Interfaces 5, Microbial Genetics & Biotechnology, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, 76344, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Biological Interfaces 5, Microbial Genetics & Biotechnology, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, 76344, Germany","institution_ids":["https://openalex.org/I102335020"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5051575088","display_name":"Dirk Holtmann","orcid":"https://orcid.org/0000-0001-5540-3550","observed_orcids":["https://orcid.org/0000-0001-5540-3550","https://orcid.org/0009-0003-7361-7407","https://orcid.org/0000-0002-8900-5216","https://orcid.org/0000-0002-2364-0225"]},"institutions":[{"id":"https://openalex.org/I102335020","display_name":"Karlsruhe Institute of Technology","ror":"https://ror.org/04t3en479","country_code":"DE","type":"education","lineage":["https://openalex.org/I102335020","https://openalex.org/I1305996414"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Dirk Holtmann","raw_affiliation_strings":["Process Engineering in Life Science, Electrobiotechnology, Karlsruhe Institute of Technology, Karlsruhe, 76131, Germany"],"raw_orcid":"https://orcid.org/0000-0001-5540-3550","affiliations":[{"raw_affiliation_string":"Process Engineering in Life Science, Electrobiotechnology, Karlsruhe Institute of Technology, Karlsruhe, 76131, Germany","institution_ids":["https://openalex.org/I102335020"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5051575088"],"corresponding_institution_ids":["https://openalex.org/I102335020"],"apc_list":{"value":3590,"currency":"USD","value_usd":3590},"apc_paid":{"value":3590,"currency":"USD","value_usd":3590},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cupriavidus-necator","display_name":"Cupriavidus necator","score":0.906000018119812},{"id":"https://openalex.org/keywords/trehalose-production","display_name":"trehalose production","score":0.9649999737739563},{"id":"https://openalex.org/keywords/adaptive-laboratory-evolution","display_name":"adaptive laboratory evolution","score":0.9909999966621399},{"id":"https://openalex.org/keywords/osmotic-adaptation","display_name":"osmoadaptation","score":0.890999972820282},{"id":"https://openalex.org/keywords/sodium-salts","display_name":"sodium salts","score":0.6919999718666077},{"id":"https://openalex.org/keywords/sodium-chloride","display_name":"sodium chloride","score":0.6299999952316284},{"id":"https://openalex.org/keywords/sodium-sulfate","display_name":"sodium sulfate","score":0.9319999814033508},{"id":"https://openalex.org/keywords/osmolytes","display_name":"osmolytes","score":0.7820000052452087},{"id":"https://openalex.org/keywords/osmotic-stress","display_name":"osmotic stress","score":0.718999981880188},{"id":"https://openalex.org/keywords/fructose-fermentation","display_name":"fructose fermentation","score":0.597000002861023}],"concepts":[{"id":"https://openalex.org/C2780637011","wikidata":"https://www.wikidata.org/wiki/Q148753","display_name":"Cupriavidus necator","level":4,"score":0.7789000272750854},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6550999879837036},{"id":"https://openalex.org/C2778452849","wikidata":"https://www.wikidata.org/wiki/Q421773","display_name":"Trehalose","level":2,"score":0.5512999892234802},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4909000098705292},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.45320001244544983},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.4399999976158142},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.40689998865127563},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.3880000114440918},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.38199999928474426},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.3580999970436096},{"id":"https://openalex.org/C125235067","wikidata":"https://www.wikidata.org/wiki/Q128700","display_name":"Cell wall","level":2,"score":0.31439998745918274},{"id":"https://openalex.org/C2910521807","wikidata":"https://www.wikidata.org/wiki/Q424469","display_name":"Poly-3-hydroxybutyrate","level":2,"score":0.30809998512268066},{"id":"https://openalex.org/C2778348673","wikidata":"https://www.wikidata.org/wiki/Q739302","display_name":"Production (economics)","level":2,"score":0.30489999055862427},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.2897000014781952},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2759000062942505},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.2667999863624573},{"id":"https://openalex.org/C175605896","wikidata":"https://www.wikidata.org/wiki/Q39833","display_name":"Microorganism","level":3,"score":0.2615000009536743},{"id":"https://openalex.org/C2779742124","wikidata":"https://www.wikidata.org/wiki/Q201552","display_name":"Inulin","level":2,"score":0.2612999975681305},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.25619998574256897}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1186/s12934-026-03167-8","doi":"https://doi.org/10.1186/s12934-026-03167-8","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12934-026-03167-8","pdf_url":null,"source":{"id":"https://openalex.org/S171368329","display_name":"Microbial Cell Factories","issn_l":"1475-2859","issn":["1475-2859"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbial Cell Factories","raw_type":"journal-article"},{"id":"doi:10.5445/ir/1000198913","doi":"https://doi.org/10.5445/ir/1000198913","is_oa":true,"landing_page_url":"https://doi.org/10.5445/ir/1000198913","pdf_url":null,"source":{"id":"https://openalex.org/S7407052948","display_name":"KITopen","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I102335020","host_organization_name":"Karlsruhe Institute of Technology","host_organization_lineage":["https://openalex.org/I102335020"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"doi:10.1186/s12934-026-03167-8","doi":"https://doi.org/10.1186/s12934-026-03167-8","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12934-026-03167-8","pdf_url":null,"source":{"id":"https://openalex.org/S171368329","display_name":"Microbial Cell Factories","issn_l":"1475-2859","issn":["1475-2859"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbial Cell Factories","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.40450000762939453}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2327343097","https://openalex.org/W3182699983","https://openalex.org/W7220793267","https://openalex.org/W6940479557","https://openalex.org/W3026164859","https://openalex.org/W4412074244","https://openalex.org/W2978792681","https://openalex.org/W2794868711","https://openalex.org/W2331368697","https://openalex.org/W4399827781"],"abstract_inverted_index":{"Trehalose":[0],"is":[1,21],"an":[2,32,274],"important":[3],"compatible":[4],"solute":[5],"with":[6,24,61,207,228,248,302],"diverse":[7],"applications":[8],"in":[9,154,298],"the":[10,90,93,124,128,144,155,175,189,192,255,258],"pharmaceutical,":[11],"food,":[12],"and":[13,64,70,120,152,167,223,235],"cosmetic":[14],"industries.":[15],"In":[16,34,85,254],"Cupriavidus":[17,215],"necator,":[18],"trehalose":[19,105,121,135,183,210,259,280,289],"biosynthesis":[20],"naturally":[22],"associated":[23,301],"osmotic":[25,56,116,276],"stress,":[26],"but":[27],"typically":[28],"retained":[29],"intracellularly":[30],"as":[31,43,113,196,242],"osmo-protectant.":[33],"this":[35],"study,":[36],"adaptive":[37],"laboratory":[38],"evolution":[39],"(ALE)":[40],"was":[41,59,101,218,240,262],"used":[42,241],"a":[44,197,243,265],"whole":[45,198,244],"cell":[46,199,245],"engineering":[47,200,246],"strategy":[48,201,247],"to":[49,54,77,103,141,149,174,220],"adapt":[50],"C.":[51,204],"necator":[52,205,216],"H16":[53,217],"increasing":[55,66,221],"stress.":[57],"ALE":[58,195,227,239],"performed":[60],"independent":[62],"lineages":[63],"gradually":[65],"concentrations":[67,108,225,230,269],"of":[68,89,109,143,194,231,270],"NaCl":[69,80,222,234],"Na\u2082SO\u2084,":[71],"reaching":[72],"stable":[73],"growth":[74,119],"at":[75],"up":[76],"500":[78,232],"mM":[79,83,233,237,272],"or":[81],"400":[82,236],"Na\u2082SO\u2084.":[84],"selected":[86,125,283],"single":[87],"colonies":[88],"adapted":[91,130,219,256],"cultures,":[92],"SetA":[94,261],"sugar":[95],"efflux":[96],"transporter":[97],"from":[98,136,290],"Escherichia":[99],"coli":[100],"expressed":[102,263],"enable":[104],"secretion.":[106],"Moderate":[107,267],"Na\u2082SO\u2084":[110],"were":[111,185],"identified":[112],"most":[114],"effective":[115,275],"trigger,":[117],"balancing":[118],"production.":[122,281],"Under":[123],"production":[126,184,211],"conditions":[127],"best-performing":[129],"isolate":[131,285],"produced":[132,286],"11.5":[133,287],"g/L":[134,138,151,288,292],"20":[137,291],"fructose,":[139],"corresponding":[140],"60.5%":[142],"theoretical":[145],"maximum":[146],"yield,":[147],"compared":[148],"7.4":[150],"42.1%":[153],"parental":[156],"strain.":[157],"Genome":[158,294],"sequencing":[159,295],"revealed":[160,296],"mutations":[161,170,297],"affecting":[162],"transporters,":[163],"membrane":[164],"structure,":[165],"metabolism":[166],"regulators.":[168],"These":[169],"are":[171],"considered":[172],"contributors":[173],"observed":[176],"osmoadaptive":[177],"phenotype,":[178],"while":[179],"individual":[180],"effects":[181],"on":[182],"not":[186],"resolved.":[187],"Overall,":[188],"results":[190],"demonstrate":[191],"potential":[193],"for":[202,251,278],"generating":[203],"isolates":[206],"improved":[208],"extracellular":[209],"under":[212],"defined":[213],"conditions.":[214],"Na2SO4":[224,268],"by":[226],"final":[229],"Na2SO4.":[238],"subsequent":[249],"selection":[250],"osmotic-stress":[252],"resistance.":[253],"isolate,":[257],"exporter":[260],"using":[264],"plasmid.":[266],"150":[271],"provided":[273],"trigger":[277],"enhanced":[279],"The":[282],"NaCl-adapted":[284],"fructose.":[293],"genes":[299],"potentially":[300],"osmoadaptation.":[303]},"counts_by_year":[],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2026-11-08T00:00:00"}