{"id":"https://openalex.org/W7225412799","doi":"https://doi.org/10.1128/spectrum.01523-26","title":"Mycoplasma genitalium extracellular vesicles and the P140 adhesin carboxyl region as high-fidelity antigens for improved serodiagnosis","display_name":"Mycoplasma genitalium extracellular vesicles and the P140 adhesin carboxyl region as high-fidelity antigens for improved serodiagnosis","publication_year":2026,"publication_date":"2026-11-06","ids":{"openalex":"https://openalex.org/W7225412799","doi":"https://doi.org/10.1128/spectrum.01523-26","pmid":"https://pubmed.ncbi.nlm.nih.gov/42992665"},"language":"en","primary_location":{"id":"doi:10.1128/spectrum.01523-26","doi":"https://doi.org/10.1128/spectrum.01523-26","is_oa":true,"landing_page_url":"https://doi.org/10.1128/spectrum.01523-26","pdf_url":null,"source":{"id":"https://openalex.org/S2764766708","display_name":"Microbiology Spectrum","issn_l":"2165-0497","issn":["2165-0497"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology Spectrum","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1128/spectrum.01523-26","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5070728608","display_name":"K. Sreenath","orcid":"https://orcid.org/0000-0002-0507-1970","observed_orcids":["https://orcid.org/0000-0002-0507-1970"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"K. Sreenath","raw_affiliation_strings":["Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio"],"raw_orcid":"https://orcid.org/0000-0002-0507-1970","affiliations":[{"raw_affiliation_string":"Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5120437044","display_name":"Danielle Molotsky","orcid":"https://orcid.org/0000-0002-2610-5711","observed_orcids":["https://orcid.org/0000-0002-2610-5711"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Danielle Molotsky","raw_affiliation_strings":["Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112773667","display_name":"Te-Hung Chang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Te-Hung Chang","raw_affiliation_strings":["Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161113043","display_name":"Murry Stein","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Murry Stein","raw_affiliation_strings":["Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090427990","display_name":"Marianna P. Cagle","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marianna Cagle","raw_affiliation_strings":["Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161001288","display_name":"Emma Jackson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Emma Jackson","raw_affiliation_strings":["Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088229750","display_name":"Joel Barry Baseman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joel B. Baseman","raw_affiliation_strings":["Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5053471489","display_name":"Thirumalai R. Kannan","orcid":"https://orcid.org/0000-0003-4590-3951","observed_orcids":["https://orcid.org/0000-0003-4590-3951"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T. R. Kannan","raw_affiliation_strings":["Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio"],"raw_orcid":"https://orcid.org/0000-0003-4590-3951","affiliations":[{"raw_affiliation_string":"Department of Microbiology, Immunology and Molecular Genetics, The University of Texas Health at San Antonio","institution_ids":["https://openalex.org/I165951966"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I165951966"],"apc_list":{"value":2500,"currency":"USD","value_usd":2500},"apc_paid":{"value":2500,"currency":"USD","value_usd":2500},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"e0152326","last_page":"e0152326"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10428","display_name":"Reproductive tract infections research","score":0.8734999895095825,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10428","display_name":"Reproductive tract infections research","score":0.8734999895095825,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.10329999774694443,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10146","display_name":"Cervical Cancer and HPV Research","score":0.004100000020116568,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mycoplasma-genitalium","display_name":"Mycoplasma genitalium","score":0.9990000128746033},{"id":"https://openalex.org/keywords/extracellular-vesicles","display_name":"extracellular vesicles","score":0.996999979019165},{"id":"https://openalex.org/keywords/serological-diagnosis","display_name":"serological diagnosis","score":0.9340000152587891},{"id":"https://openalex.org/keywords/diagnostic-antigen","display_name":"diagnostic antigen","score":0.7879999876022339},{"id":"https://openalex.org/keywords/host-cell-adhesion","display_name":"host cell adhesion","score":0.734000027179718},{"id":"https://openalex.org/keywords/cross-reactivity","display_name":"cross-reactivity","score":0.6140000224113464},{"id":"https://openalex.org/keywords/epitope-specific-antibodies","display_name":"epitope-specific antibodies","score":0.5950000286102295},{"id":"https://openalex.org/keywords/infection-clearance","display_name":"infection clearance","score":0.6880000233650208},{"id":"https://openalex.org/keywords/sexually-transmitted-infections","display_name":"sexually transmitted infections","score":0.878000020980835},{"id":"https://openalex.org/keywords/cervical-epithelial-cells","display_name":"cervical epithelial cells","score":0.7179999947547913},{"id":"https://openalex.org/keywords/antigenic-variation","display_name":"antigenic variation","score":0.3569999933242798}],"concepts":[{"id":"https://openalex.org/C2780062933","wikidata":"https://www.wikidata.org/wiki/Q1930909","display_name":"Mycoplasma genitalium","level":3,"score":0.8618999719619751},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.7692000269889832},{"id":"https://openalex.org/C179470777","wikidata":"https://www.wikidata.org/wiki/Q356661","display_name":"Bacterial adhesin","level":4,"score":0.7487999796867371},{"id":"https://openalex.org/C45189115","wikidata":"https://www.wikidata.org/wiki/Q502159","display_name":"Serology","level":3,"score":0.6890000104904175},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5432000160217285},{"id":"https://openalex.org/C2781151156","wikidata":"https://www.wikidata.org/wiki/Q150185","display_name":"Mycoplasma gallisepticum","level":3,"score":0.5206000208854675},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5094000101089478},{"id":"https://openalex.org/C32611913","wikidata":"https://www.wikidata.org/wiki/Q2166189","display_name":"Titer","level":3,"score":0.5044000148773193},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.4666999876499176},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.4408000111579895},{"id":"https://openalex.org/C2992929900","wikidata":"https://www.wikidata.org/wiki/Q21097485","display_name":"Extracellular vesicles","level":2,"score":0.40860000252723694},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.40540000796318054},{"id":"https://openalex.org/C2779026604","wikidata":"https://www.wikidata.org/wiki/Q4774742","display_name":"Antigenicity","level":3,"score":0.39329999685287476},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.35359999537467957},{"id":"https://openalex.org/C22889606","wikidata":"https://www.wikidata.org/wiki/Q2166189","display_name":"Antibody titer","level":4,"score":0.33709999918937683},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.3303000032901764},{"id":"https://openalex.org/C2909760446","wikidata":"https://www.wikidata.org/wiki/Q12198","display_name":"Sexually transmitted disease","level":4,"score":0.3296000063419342},{"id":"https://openalex.org/C71829478","wikidata":"https://www.wikidata.org/wiki/Q901305","display_name":"Immunoprecipitation","level":3,"score":0.3149000108242035},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.29809999465942383},{"id":"https://openalex.org/C2778838687","wikidata":"https://www.wikidata.org/wiki/Q210975","display_name":"Mycoplasma","level":2,"score":0.29109999537467957},{"id":"https://openalex.org/C191942190","wikidata":"https://www.wikidata.org/wiki/Q4774739","display_name":"Antigenic variation","level":3,"score":0.29030001163482666},{"id":"https://openalex.org/C22070199","wikidata":"https://www.wikidata.org/wiki/Q192995","display_name":"Vaccination","level":2,"score":0.2865999937057495},{"id":"https://openalex.org/C2780122209","wikidata":"https://www.wikidata.org/wiki/Q153356","display_name":"Chlamydia","level":2,"score":0.28459998965263367},{"id":"https://openalex.org/C72563966","wikidata":"https://www.wikidata.org/wiki/Q1303415","display_name":"Cohort","level":2,"score":0.2815000116825104},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.2809000015258789},{"id":"https://openalex.org/C163764329","wikidata":"https://www.wikidata.org/wiki/Q785022","display_name":"Autoantibody","level":3,"score":0.2703999876976013},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.2676999866962433},{"id":"https://openalex.org/C13426554","wikidata":"https://www.wikidata.org/wiki/Q416019","display_name":"Glycoconjugate","level":2,"score":0.2671000063419342},{"id":"https://openalex.org/C131934819","wikidata":"https://www.wikidata.org/wiki/Q135390","display_name":"Biogenesis","level":3,"score":0.2644999921321869},{"id":"https://openalex.org/C146587185","wikidata":"https://www.wikidata.org/wiki/Q258248","display_name":"Bacterial outer membrane","level":4,"score":0.25690001249313354}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1128/spectrum.01523-26","doi":"https://doi.org/10.1128/spectrum.01523-26","is_oa":true,"landing_page_url":"https://doi.org/10.1128/spectrum.01523-26","pdf_url":null,"source":{"id":"https://openalex.org/S2764766708","display_name":"Microbiology Spectrum","issn_l":"2165-0497","issn":["2165-0497"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology Spectrum","raw_type":"journal-article"},{"id":"pmid:42992665","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42992665","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology spectrum","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1128/spectrum.01523-26","doi":"https://doi.org/10.1128/spectrum.01523-26","is_oa":true,"landing_page_url":"https://doi.org/10.1128/spectrum.01523-26","pdf_url":null,"source":{"id":"https://openalex.org/S2764766708","display_name":"Microbiology Spectrum","issn_l":"2165-0497","issn":["2165-0497"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology Spectrum","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.9537000060081482,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":47,"referenced_works":["https://openalex.org/W158388333","https://openalex.org/W1512781823","https://openalex.org/W1618885046","https://openalex.org/W1947419382","https://openalex.org/W1965421459","https://openalex.org/W2010474381","https://openalex.org/W2011168790","https://openalex.org/W2015196893","https://openalex.org/W2021845849","https://openalex.org/W2036464060","https://openalex.org/W2057394568","https://openalex.org/W2063554696","https://openalex.org/W2072067077","https://openalex.org/W2075240542","https://openalex.org/W2088507637","https://openalex.org/W2099894900","https://openalex.org/W2103493445","https://openalex.org/W2105378747","https://openalex.org/W2107201398","https://openalex.org/W2121665963","https://openalex.org/W2125342917","https://openalex.org/W2134682501","https://openalex.org/W2139296315","https://openalex.org/W2153970881","https://openalex.org/W2165782824","https://openalex.org/W2168441823","https://openalex.org/W2308145610","https://openalex.org/W2341474445","https://openalex.org/W2740092384","https://openalex.org/W2741559868","https://openalex.org/W2896002418","https://openalex.org/W2902839015","https://openalex.org/W3000054067","https://openalex.org/W3012454749","https://openalex.org/W3013446608","https://openalex.org/W3033325642","https://openalex.org/W3092190040","https://openalex.org/W3093643162","https://openalex.org/W3185444663","https://openalex.org/W4220672885","https://openalex.org/W4224124552","https://openalex.org/W4288032866","https://openalex.org/W4313489041","https://openalex.org/W4383186925","https://openalex.org/W4383874461","https://openalex.org/W4409697980","https://openalex.org/W4416792270"],"related_works":["https://openalex.org/W2108542660","https://openalex.org/W4409697980","https://openalex.org/W2107201398","https://openalex.org/W2011168790","https://openalex.org/W2745099155","https://openalex.org/W3021343384","https://openalex.org/W1927620915","https://openalex.org/W2738208995","https://openalex.org/W2787556426","https://openalex.org/W7203628183"],"abstract_inverted_index":{"ABSTRACT":[0],"Mycoplasma":[1,259],"genitalium":[2,260],"(":[3],"Mg":[4,45,60,68,115,133,221,235,279],")":[5],"is":[6],"an":[7,83,200],"important":[8],"etiological":[9],"agent":[10],"of":[11,59,86,110,252,258,269,287,322,332],"sexually":[12],"transmitted":[13],"infections":[14],"that":[15,67,71,139,304,315],"has":[16],"emerged":[17],"as":[18,164,189,237,245,284,319,329],"a":[19,48,94,107,218,238,246,285,300,312,320,330,339],"global":[20],"health":[21],"threat.":[22],"Despite":[23],"its":[24],"clinical":[25,210],"importance,":[26],"accurate":[27],"serodiagnosis":[28],"remains":[29],"challenging":[30],"due":[31],"to":[32,52],"high":[33,212],"cross-reactivity":[34],"caused":[35],"by":[36],"non-specific":[37,161],"proteins.":[38],"To":[39],"address":[40],"this":[41,196],"limitation,":[42],"we":[43,92,298],"optimized":[44],"growth":[46],"in":[47,176,204,266],"serum-free":[49],"minimal":[50],"medium":[51],"characterize":[53],"the":[54,76,140,185,190,208,250,267,294],"biogenesis":[55],"and":[56,80,106,128,135,151,172,242,273,310],"diagnostic":[57,145,334],"applications":[58],"-secreted":[61],"extracellular":[62,281],"vesicles":[63,282],"(EVs).":[64],"We":[65],"demonstrate":[66],"generates":[69],"EVs":[70,236,328],"retain":[72],"immunodominant":[73],"proteins,":[74,162],"specifically":[75],"key":[77],"adhesins":[78],"P140":[79,174,295],"P110.":[81],"From":[82],"initial":[84],"cohort":[85],"1,608":[87],"women":[88,100],"(12.5%":[89],"Mg-":[90],"positive),":[91],"selected":[93],"longitudinal":[95,209],"sub-study":[96],"population,":[97],"including":[98],"24":[99],"who":[101,113],"were":[102],"PCR-positive":[103],"at":[104],"enrollment":[105],"control":[108],"group":[109],"14":[111],"individuals":[112,137],"remained":[114],"-negative":[116],"across":[117],"three":[118],"visits,":[119],"each":[120],"spaced":[121],"approximately":[122],"6":[123],"months":[124],"apart.":[125],"Comparative":[126],"ELISA":[127],"immunoblotting":[129],"using":[130,170],"sera":[131],"from":[132],"-infected":[134],"uninfected":[136],"revealed":[138],"EV-associated":[141,157],"antigens":[142,158,289],"provide":[143],"superior":[144],"discrimination":[146],"over":[147],"conventional":[148],"total-cell":[149],"lysates":[150],"lipid-associated":[152],"membrane":[153],"protein":[154],"extracts.":[155],"Notably,":[156],"lack":[159],"common":[160],"such":[163],"immunoglobulin-binding":[165],"protein,":[166],"MG281.":[167],"Functional":[168],"characterization":[169],"full-length":[171],"truncated":[173],"variants":[175],"cell-based":[177],"ELISAs":[178],"with":[179,217,341],"human":[180],"cervical":[181],"epithelial":[182],"cells":[183],"identified":[184,299],"C-terminal":[186,296],"region":[187,198],"(P140-C)":[188],"primary":[191],"host-cell-interacting":[192],"domain.":[193],"In":[194],"addition,":[195],"specific":[197,302],"induces":[199],"epitope-restricted":[201],"antibody":[202,214],"response":[203],"infected":[205],"individuals.":[206],"Within":[207],"cohort,":[211],"anti-P140-C":[213],"titers":[215],"correlated":[216],"trend":[219],"toward":[220],"clearance,":[222],"suggesting":[223],"potential":[224],"prognostic":[225],"utility":[226],"despite":[227],"lacking":[228],"statistical":[229],"significance.":[230],"Collectively,":[231],"these":[232],"results":[233],"establish":[234],"high-fidelity":[239],"antigenic":[240,306],"source":[241,286,331],"identify":[243],"P140-C":[244],"promising":[247],"candidate":[248,313],"for":[249,290,344],"development":[251],"next-generation":[253],"serological":[254],"assays.":[255],"IMPORTANCE":[256],"Serodiagnosis":[257],"(Mg)":[261],"faces":[262],"significant":[263],"challenges,":[264],"especially":[265],"context":[268],"emerging":[270],"antimicrobial":[271],"resistance":[272],"asymptomatic":[274],"infections.":[275],"This":[276],"study":[277],"introduces":[278],"-derived":[280],"(EVs)":[283],"refined":[288],"serodiagnosis.":[291],"By":[292,325],"isolating":[293],"region,":[297],"highly":[301],"marker":[303,321],"overcomes":[305],"variation,":[307],"reduces":[308],"cross-reactivity,":[309],"offers":[311],"antigen":[314],"may":[316],"warrant":[317],"evaluation":[318],"infection":[323],"clearance.":[324],"leveraging":[326],"bacterial":[327],"purified":[333],"antigen,":[335],"our":[336],"findings":[337],"offer":[338],"framework":[340],"broader":[342],"implications":[343],"other":[345],"antigenically":[346],"complex":[347],"pathogens.":[348]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-11-07T00:00:00"}