{"id":"https://openalex.org/W7225410940","doi":"https://doi.org/10.64898/2026.11.04.764386","title":"From Fill to Freeze: Redesigning homeRNA to Enable Robust Sampling and Scalable Downstream Processing Workflows for Remote Blood Transcriptomics","display_name":"From Fill to Freeze: Redesigning homeRNA to Enable Robust Sampling and Scalable Downstream Processing Workflows for Remote Blood Transcriptomics","publication_year":2026,"publication_date":"2026-11-06","ids":{"openalex":"https://openalex.org/W7225410940","doi":"https://doi.org/10.64898/2026.11.04.764386"},"language":"en","primary_location":{"id":"doi:10.64898/2026.11.04.764386","doi":"https://doi.org/10.64898/2026.11.04.764386","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.04.764386","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/06/2026.11.04.764386.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/06/2026.11.04.764386.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5012481470","display_name":"Madeleine Eakman","orcid":"https://orcid.org/0009-0000-0084-0171","observed_orcids":["https://orcid.org/0009-0000-0084-0171"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Madeleine Eakman","raw_affiliation_strings":["University of Washington;"],"raw_orcid":"https://orcid.org/0009-0000-0084-0171","affiliations":[{"raw_affiliation_string":"University of Washington;","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010043769","display_name":"Ingrid H. Robertson","orcid":"https://orcid.org/0009-0008-4217-4777","observed_orcids":["https://orcid.org/0009-0008-4217-4777","https://orcid.org/0000-0001-6666-3876"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ingrid H. Robertson","raw_affiliation_strings":["University of Washington;"],"raw_orcid":"https://orcid.org/0009-0008-4217-4777","affiliations":[{"raw_affiliation_string":"University of Washington;","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108309520","display_name":"Xiaofu Wei","orcid":"https://orcid.org/0009-0007-8618-4750","observed_orcids":["https://orcid.org/0009-0007-8618-4750","https://orcid.org/0009-0005-2565-913X"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xiaofu Wei","raw_affiliation_strings":["University of Washington;"],"raw_orcid":"https://orcid.org/0009-0007-8618-4750","affiliations":[{"raw_affiliation_string":"University of Washington;","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058481550","display_name":"Filip Stefanovic","orcid":"https://orcid.org/0000-0002-1448-9578","observed_orcids":["https://orcid.org/0000-0002-1448-9578"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Filip Stefanovic","raw_affiliation_strings":["University of Washington;"],"raw_orcid":"https://orcid.org/0000-0002-1448-9578","affiliations":[{"raw_affiliation_string":"University of Washington;","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5161092079","display_name":"TJ Caira","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"TJ Caira","raw_affiliation_strings":["University of Washington;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Washington;","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059000541","display_name":"David J. Guckenberger","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"David J. Guckenberger","raw_affiliation_strings":["Seabright LLC"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Seabright LLC","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005601629","display_name":"Karen N. Adams","orcid":"https://orcid.org/0000-0002-7457-0175","observed_orcids":["https://orcid.org/0000-0002-7457-0175"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Karen N. Adams","raw_affiliation_strings":["University of Washington;"],"raw_orcid":"https://orcid.org/0000-0002-7457-0175","affiliations":[{"raw_affiliation_string":"University of Washington;","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101612696","display_name":"Erwin Berthier","orcid":"https://orcid.org/0000-0002-4421-9034","observed_orcids":["https://orcid.org/0000-0002-4421-9034"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Erwin Berthier","raw_affiliation_strings":["Seabright LLC"],"raw_orcid":"https://orcid.org/0000-0002-4421-9034","affiliations":[{"raw_affiliation_string":"Seabright LLC","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043772176","display_name":"Lauren G. Brown","orcid":"https://orcid.org/0000-0002-5932-2691","observed_orcids":["https://orcid.org/0000-0002-5932-2691"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lauren G. Brown","raw_affiliation_strings":["Seabright LLC"],"raw_orcid":"https://orcid.org/0000-0002-5932-2691","affiliations":[{"raw_affiliation_string":"Seabright LLC","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016523732","display_name":"Sanitta Thongpang","orcid":"https://orcid.org/0000-0002-7920-2080","observed_orcids":["https://orcid.org/0000-0002-7920-2080"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sanitta Thongpang","raw_affiliation_strings":["Seabright LLC"],"raw_orcid":"https://orcid.org/0000-0002-7920-2080","affiliations":[{"raw_affiliation_string":"Seabright LLC","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077206096","display_name":"Ashleigh B. Theberge","orcid":"https://orcid.org/0000-0002-3394-1435","observed_orcids":["https://orcid.org/0000-0002-3394-1435"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ashleigh B Theberge","raw_affiliation_strings":["University of Washington;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Washington;","institution_ids":["https://openalex.org/I201448701"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5077206096"],"corresponding_institution_ids":["https://openalex.org/I201448701"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.7821999788284302,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.7821999788284302,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11314","display_name":"Clinical Laboratory Practices and Quality Control","score":0.0608999989926815,"subfield":{"id":"https://openalex.org/subfields/3607","display_name":"Medical Laboratory Technology"},"field":{"id":"https://openalex.org/fields/36","display_name":"Health Professions"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10582","display_name":"Ethics in Clinical Research","score":0.03739999979734421,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/blood-transcriptomics","display_name":"blood transcriptomics","score":0.7620000243186951},{"id":"https://openalex.org/keywords/rna-stabilization","display_name":"RNA stabilization","score":0.9409999847412109},{"id":"https://openalex.org/keywords/rna-quality","display_name":"RNA quality","score":0.6439999938011169},{"id":"https://openalex.org/keywords/sample-storage-stability","display_name":"sample storage stability","score":0.453000009059906},{"id":"https://openalex.org/keywords/high-throughput-manufacturing","display_name":"high-throughput fabrication","score":0.7160000205039978}],"concepts":[{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.760200023651123},{"id":"https://openalex.org/C48044578","wikidata":"https://www.wikidata.org/wiki/Q727490","display_name":"Scalability","level":2,"score":0.6883000135421753},{"id":"https://openalex.org/C170130773","wikidata":"https://www.wikidata.org/wiki/Q216378","display_name":"Usability","level":2,"score":0.61080002784729},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5680000185966492},{"id":"https://openalex.org/C2776207758","wikidata":"https://www.wikidata.org/wiki/Q5303302","display_name":"Downstream (manufacturing)","level":2,"score":0.5394999980926514},{"id":"https://openalex.org/C79403827","wikidata":"https://www.wikidata.org/wiki/Q3988","display_name":"Real-time computing","level":1,"score":0.49880000948905945},{"id":"https://openalex.org/C140779682","wikidata":"https://www.wikidata.org/wiki/Q210868","display_name":"Sampling (signal processing)","level":3,"score":0.43689998984336853},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.40549999475479126},{"id":"https://openalex.org/C39432304","wikidata":"https://www.wikidata.org/wiki/Q188847","display_name":"Environmental science","level":0,"score":0.34860000014305115},{"id":"https://openalex.org/C77088390","wikidata":"https://www.wikidata.org/wiki/Q8513","display_name":"Database","level":1,"score":0.3334999978542328},{"id":"https://openalex.org/C146849305","wikidata":"https://www.wikidata.org/wiki/Q370766","display_name":"Ground truth","level":2,"score":0.3093999922275543},{"id":"https://openalex.org/C133462117","wikidata":"https://www.wikidata.org/wiki/Q4929239","display_name":"Data collection","level":2,"score":0.2831999957561493},{"id":"https://openalex.org/C19012869","wikidata":"https://www.wikidata.org/wiki/Q578372","display_name":"Response time","level":2,"score":0.27379998564720154},{"id":"https://openalex.org/C21880701","wikidata":"https://www.wikidata.org/wiki/Q2144042","display_name":"Process engineering","level":1,"score":0.2727999985218048},{"id":"https://openalex.org/C18762648","wikidata":"https://www.wikidata.org/wiki/Q42213","display_name":"Work (physics)","level":2,"score":0.27239999175071716},{"id":"https://openalex.org/C44154836","wikidata":"https://www.wikidata.org/wiki/Q45045","display_name":"Simulation","level":1,"score":0.26660001277923584},{"id":"https://openalex.org/C24756922","wikidata":"https://www.wikidata.org/wiki/Q1757694","display_name":"Data quality","level":3,"score":0.2621000111103058},{"id":"https://openalex.org/C2779530757","wikidata":"https://www.wikidata.org/wiki/Q1207505","display_name":"Quality (philosophy)","level":2,"score":0.2597000002861023},{"id":"https://openalex.org/C63479239","wikidata":"https://www.wikidata.org/wiki/Q7353546","display_name":"Robustness (evolution)","level":3,"score":0.25380000472068787},{"id":"https://openalex.org/C206729178","wikidata":"https://www.wikidata.org/wiki/Q2271896","display_name":"Scheduling (production processes)","level":2,"score":0.25290000438690186}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.11.04.764386","doi":"https://doi.org/10.64898/2026.11.04.764386","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.04.764386","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/06/2026.11.04.764386.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.11.04.764386","doi":"https://doi.org/10.64898/2026.11.04.764386","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.04.764386","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/06/2026.11.04.764386.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1790327027","display_name":null,"funder_award_id":"TL1TR002318","funder_id":"https://openalex.org/F4320306085","funder_display_name":"U.S. Department of Health and Human Services"},{"id":"https://openalex.org/G2100657707","display_name":null,"funder_award_id":"UL1TR002319","funder_id":"https://openalex.org/F4320306085","funder_display_name":"U.S. Department of Health and Human Services"},{"id":"https://openalex.org/G5086723997","display_name":null,"funder_award_id":"UL1TR002319","funder_id":"https://openalex.org/F4320314220","funder_display_name":"Institute of Translational Health Sciences"},{"id":"https://openalex.org/G9000858468","display_name":"NRSA Training Core","funder_award_id":"TL1TR002318","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000897255","display_name":"Institute of Translational Health Sciences","funder_award_id":"UL1TR002319","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320306085","display_name":"U.S. Department of Health and Human Services","ror":"https://ror.org/033jnv181"},{"id":"https://openalex.org/F4320314220","display_name":"Institute of Translational Health Sciences","ror":"https://ror.org/01cp21a44"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337472","display_name":"National Center for Advancing Translational Sciences","ror":"https://ror.org/04pw6fb54"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225410940.pdf","grobid_xml":"https://content.openalex.org/works/W7225410940.grobid-xml"},"referenced_works_count":24,"referenced_works":["https://openalex.org/W1696199194","https://openalex.org/W1974767214","https://openalex.org/W2060842569","https://openalex.org/W2074871118","https://openalex.org/W2092799262","https://openalex.org/W2152448137","https://openalex.org/W2165482500","https://openalex.org/W2461654689","https://openalex.org/W2564672923","https://openalex.org/W2624605702","https://openalex.org/W2755142955","https://openalex.org/W2948075606","https://openalex.org/W3114014715","https://openalex.org/W3200742161","https://openalex.org/W3209351892","https://openalex.org/W4292452043","https://openalex.org/W4378070774","https://openalex.org/W4386893947","https://openalex.org/W4389160860","https://openalex.org/W4400968119","https://openalex.org/W4406492492","https://openalex.org/W4406561888","https://openalex.org/W4416711482","https://openalex.org/W7214113763"],"related_works":["https://openalex.org/W4416711482","https://openalex.org/W4292452043","https://openalex.org/W7214113763","https://openalex.org/W4406492492","https://openalex.org/W3200742161","https://openalex.org/W7125233133","https://openalex.org/W4404580803","https://openalex.org/W2092799262","https://openalex.org/W3177332861","https://openalex.org/W3150265263"],"abstract_inverted_index":{"In-person":[0],"research":[1],"can":[2,90,114,194,244],"introduce":[3],"barriers":[4],"to":[5,9,22,52,102,205,223,236],"participation,":[6],"including":[7],"proximity":[8],"a":[10,45,93],"study":[11,95],"site":[12],"and":[13,30,59,68,104,117,161,168,202],"scheduling":[14],"constraints.":[15],"Our":[16],"lab":[17],"developed":[18,49],"the":[19,38,71,75,111,140,230,239],"homeRNA":[20,41,112,141,232],"platform":[21,142,233],"address":[23],"these":[24],"barriers,":[25],"enabling":[26],"remote":[27,94,247],"blood":[28,176,184],"collection":[29],"RNA":[31,144,170],"stabilization":[32],"for":[33,42,56,65,148,209],"transcriptomic":[34,150],"research.":[35],"To":[36],"increase":[37],"applicability":[39],"of":[40,74,128,145,219,238],"large-scale":[43],"studies,":[44],"redesigned":[46],"version":[47],"was":[48],"with":[50,130],"modifications":[51],"1)":[53],"improve":[54],"usability":[55],"both":[57],"participants":[58,180],"downstream":[60,149],"processing":[61],"laboratories,":[62],"2)":[63],"allow":[64],"high-throughput":[66],"manufacturing,":[67],"3)":[69],"extend":[70],"shelf":[72,132],"life":[73,133],"stabilizer":[76],"tube.":[77],"In":[78],"this":[79,84],"work,":[80],"we":[81,189],"systematically":[82],"tested":[83],"design":[85],"against":[86],"anticipated":[87],"stresses":[88,241],"that":[89,110,139,169,192,229,242],"occur":[91],"throughout":[92],"(e.g.,":[96],"high":[97],"temperature":[98,121,163,208],"exposure":[99],"during":[100,246],"shipment)":[101],"identify":[103],"characterize":[105],"its":[106],"limitations.":[107],"We":[108,136],"found":[109,138,191],"device":[113],"be":[115,195],"prepared":[116],"stored":[118,196],"at":[119,122,210,221],"room":[120,207],"least":[123,211],"three":[124],"months":[125],"in":[126,159,183,197],"advance":[127],"sampling":[129],"continued":[131],"analysis":[134,151],"underway.":[135],"also":[137,190],"produces":[143],"sufficient":[146],"quality":[147,171],"under":[152],"standard":[153,206],"shipping":[154],"durations":[155],"(i.e.,":[156,165],"2":[157],"days":[158],"transit)":[160],"ambient-to-high":[162],"conditions":[164],"25-40":[166],"\u00b0C)":[167],"is":[172,234],"unaffected":[173],"by":[174,179],"variable":[175],"volumes":[177,185],"collected":[178],"(mean":[181,214],"RIN=7.8":[182],"0.25-1":[186],"mL).":[187],"Lastly,":[188],"samples":[193,243],"typical":[198],"cold":[199],"storage":[200,220],"temperatures":[201],"even":[203],"up":[204],"4":[212,217],"weeks":[213,218],"RIN=8.1":[215],"after":[216],"-80":[222],"20":[224],"\u00b0C).":[225],"This":[226],"work":[227],"validates":[228],"new":[231],"robust":[235],"many":[237],"expected":[240],"experience":[245],"studies.":[248]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2026-11-07T00:00:00"}