{"id":"https://openalex.org/W7225339972","doi":"https://doi.org/10.1007/s44372-026-00994-2","title":"Effect of TDZ treatment on the regeneration of shoots in recalcitrant grain legumes: Cicer arietinum, Glycine max, Phaseolus vulgaris, and Vigna unguiculata","display_name":"Effect of TDZ treatment on the regeneration of shoots in recalcitrant grain legumes: Cicer arietinum, Glycine max, Phaseolus vulgaris, and Vigna unguiculata","publication_year":2026,"publication_date":"2026-11-05","ids":{"openalex":"https://openalex.org/W7225339972","doi":"https://doi.org/10.1007/s44372-026-00994-2"},"language":"en","primary_location":{"id":"doi:10.1007/s44372-026-00994-2","doi":"https://doi.org/10.1007/s44372-026-00994-2","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s44372-026-00994-2","pdf_url":"https://link.springer.com/content/pdf/10.1007/s44372-026-00994-2.pdf","source":{"id":"https://openalex.org/S4404533546","display_name":"Discover Plants.","issn_l":"3005-1207","issn":["3005-1207"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["doaj"],"host_organization":"https://openalex.org/P4310317082","host_organization_name":"Academy of Medicine, Singapore","host_organization_lineage":["https://openalex.org/P4310317082"],"host_organization_lineage_names":["Academy of Medicine, Singapore"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Discover Plants","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://link.springer.com/content/pdf/10.1007/s44372-026-00994-2.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017105726","display_name":"Renu Soni","orcid":"https://orcid.org/0000-0002-9351-5323","observed_orcids":["https://orcid.org/0000-0002-9351-5323"]},"institutions":[{"id":"https://openalex.org/I110166357","display_name":"University of Delhi","ror":"https://ror.org/04gzb2213","country_code":"IN","type":"education","lineage":["https://openalex.org/I110166357"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Renu Soni","raw_affiliation_strings":["Department of Botany, Gargi College, University of Delhi, Delhi, 110049, India"],"raw_orcid":"https://orcid.org/0000-0002-9351-5323","affiliations":[{"raw_affiliation_string":"Department of Botany, Gargi College, University of Delhi, Delhi, 110049, India","institution_ids":["https://openalex.org/I110166357"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024944162","display_name":"Gayatri Tripathi","orcid":"https://orcid.org/0000-0002-1782-7931","observed_orcids":["https://orcid.org/0000-0002-1782-7931"]},"institutions":[{"id":"https://openalex.org/I110166357","display_name":"University of Delhi","ror":"https://ror.org/04gzb2213","country_code":"IN","type":"education","lineage":["https://openalex.org/I110166357"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Gayatri Tripathi","raw_affiliation_strings":["Department of Plant Molecular Biology, University of Delhi South Campus, Delhi, 110021, India"],"raw_orcid":"https://orcid.org/0000-0002-1782-7931","affiliations":[{"raw_affiliation_string":"Department of Plant Molecular Biology, University of Delhi South Campus, Delhi, 110021, India","institution_ids":["https://openalex.org/I110166357"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5160786017","display_name":"Nishika Ravi","orcid":"https://orcid.org/0009-0002-8078-6826","observed_orcids":["https://orcid.org/0009-0002-8078-6826"]},"institutions":[{"id":"https://openalex.org/I110166357","display_name":"University of Delhi","ror":"https://ror.org/04gzb2213","country_code":"IN","type":"education","lineage":["https://openalex.org/I110166357"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Nishika Ravi","raw_affiliation_strings":["Department of Botany, University of Delhi, Delhi, 110007, India"],"raw_orcid":"https://orcid.org/0009-0002-8078-6826","affiliations":[{"raw_affiliation_string":"Department of Botany, University of Delhi, Delhi, 110007, India","institution_ids":["https://openalex.org/I110166357"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5017105726","https://openalex.org/A5024944162","https://openalex.org/A5160786017"],"corresponding_institution_ids":["https://openalex.org/I110166357"],"apc_list":{"value":0,"currency":"USD","value_usd":0},"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"3","issue":"1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12834","display_name":"Flowering Plant Growth and Cultivation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thidiazuron","display_name":"thidiazuron","score":0.8149999976158142},{"id":"https://openalex.org/keywords/direct-shoot-regeneration","display_name":"direct shoot regeneration","score":0.7850000262260437},{"id":"https://openalex.org/keywords/chickpea","display_name":"chickpea","score":0.9129999876022339},{"id":"https://openalex.org/keywords/soybean","display_name":"soybean","score":0.9279999732971191},{"id":"https://openalex.org/keywords/common-bean","display_name":"Phaseolus vulgaris","score":1.0},{"id":"https://openalex.org/keywords/cowpea","display_name":"cowpea","score":1.0},{"id":"https://openalex.org/keywords/genetic-transformation","display_name":"genetic transformation","score":0.6790000200271606},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.925000011920929}],"concepts":[{"id":"https://openalex.org/C2779862049","wikidata":"https://www.wikidata.org/wiki/Q132634","display_name":"Vigna","level":2,"score":0.8615000247955322},{"id":"https://openalex.org/C21410773","wikidata":"https://www.wikidata.org/wiki/Q220869","display_name":"Shoot","level":2,"score":0.7746000289916992},{"id":"https://openalex.org/C2776286235","wikidata":"https://www.wikidata.org/wiki/Q310438","display_name":"Phaseolus","level":2,"score":0.7123000025749207},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5960999727249146},{"id":"https://openalex.org/C9549007","wikidata":"https://www.wikidata.org/wiki/Q1072909","display_name":"Callus","level":2,"score":0.519599974155426},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.48910000920295715},{"id":"https://openalex.org/C171056886","wikidata":"https://www.wikidata.org/wiki/Q193119","display_name":"Regeneration (biology)","level":2,"score":0.4844000041484833},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.4169999957084656},{"id":"https://openalex.org/C157890192","wikidata":"https://www.wikidata.org/wiki/Q2561978","display_name":"Explant culture","level":3,"score":0.38109999895095825},{"id":"https://openalex.org/C125418430","wikidata":"https://www.wikidata.org/wiki/Q1754218","display_name":"Etiolation","level":3,"score":0.3531999886035919},{"id":"https://openalex.org/C2776959544","wikidata":"https://www.wikidata.org/wiki/Q156811","display_name":"Lupinus","level":2,"score":0.3125},{"id":"https://openalex.org/C200806820","wikidata":"https://www.wikidata.org/wiki/Q1798348","display_name":"Murashige and Skoog medium","level":4,"score":0.3041999936103821},{"id":"https://openalex.org/C161369605","wikidata":"https://www.wikidata.org/wiki/Q41803","display_name":"Gram","level":3,"score":0.30379998683929443},{"id":"https://openalex.org/C2778597767","wikidata":"https://www.wikidata.org/wiki/Q4027534","display_name":"Sucrose","level":2,"score":0.2768000066280365},{"id":"https://openalex.org/C2777756961","wikidata":"https://www.wikidata.org/wiki/Q620730","display_name":"Glycine","level":3,"score":0.2651999890804291},{"id":"https://openalex.org/C201468095","wikidata":"https://www.wikidata.org/wiki/Q7559239","display_name":"Somatic embryogenesis","level":4,"score":0.2639000117778778},{"id":"https://openalex.org/C2777531858","wikidata":"https://www.wikidata.org/wiki/Q3239959","display_name":"Cajanus","level":2,"score":0.26350000500679016},{"id":"https://openalex.org/C6557445","wikidata":"https://www.wikidata.org/wiki/Q173113","display_name":"Agronomy","level":1,"score":0.2621999979019165}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1007/s44372-026-00994-2","doi":"https://doi.org/10.1007/s44372-026-00994-2","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s44372-026-00994-2","pdf_url":"https://link.springer.com/content/pdf/10.1007/s44372-026-00994-2.pdf","source":{"id":"https://openalex.org/S4404533546","display_name":"Discover Plants.","issn_l":"3005-1207","issn":["3005-1207"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["doaj"],"host_organization":"https://openalex.org/P4310317082","host_organization_name":"Academy of Medicine, Singapore","host_organization_lineage":["https://openalex.org/P4310317082"],"host_organization_lineage_names":["Academy of Medicine, Singapore"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Discover Plants","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:d8be01d5a99e4784b0fb08c52c7dbfae","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/d8be01d5a99e4784b0fb08c52c7dbfae","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Discover Plants, Vol 3, Iss 1 (2026)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1007/s44372-026-00994-2","doi":"https://doi.org/10.1007/s44372-026-00994-2","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s44372-026-00994-2","pdf_url":"https://link.springer.com/content/pdf/10.1007/s44372-026-00994-2.pdf","source":{"id":"https://openalex.org/S4404533546","display_name":"Discover Plants.","issn_l":"3005-1207","issn":["3005-1207"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["doaj"],"host_organization":"https://openalex.org/P4310317082","host_organization_name":"Academy of Medicine, Singapore","host_organization_lineage":["https://openalex.org/P4310317082"],"host_organization_lineage_names":["Academy of Medicine, Singapore"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Discover Plants","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.991100013256073,"display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225339972.pdf","grobid_xml":"https://content.openalex.org/works/W7225339972.grobid-xml"},"referenced_works_count":56,"referenced_works":["https://openalex.org/W578868330","https://openalex.org/W1428406991","https://openalex.org/W1965852315","https://openalex.org/W1990406984","https://openalex.org/W2020673690","https://openalex.org/W2023885030","https://openalex.org/W2029646215","https://openalex.org/W2042288518","https://openalex.org/W2106881160","https://openalex.org/W2127585131","https://openalex.org/W2141995813","https://openalex.org/W2150747259","https://openalex.org/W2346418876","https://openalex.org/W2509776767","https://openalex.org/W2523009970","https://openalex.org/W2614129448","https://openalex.org/W2765319192","https://openalex.org/W2786709364","https://openalex.org/W2790545864","https://openalex.org/W2792715046","https://openalex.org/W2884112928","https://openalex.org/W2897326689","https://openalex.org/W2942091510","https://openalex.org/W2963980873","https://openalex.org/W3031220741","https://openalex.org/W3079393396","https://openalex.org/W3082868024","https://openalex.org/W3085146997","https://openalex.org/W3117605672","https://openalex.org/W3177673346","https://openalex.org/W3190016794","https://openalex.org/W3205733187","https://openalex.org/W4207064925","https://openalex.org/W4214673824","https://openalex.org/W4214948745","https://openalex.org/W4214950835","https://openalex.org/W4237375657","https://openalex.org/W4246481443","https://openalex.org/W4281693720","https://openalex.org/W4283795854","https://openalex.org/W4294989742","https://openalex.org/W4313209780","https://openalex.org/W4322732153","https://openalex.org/W4366772278","https://openalex.org/W4385985385","https://openalex.org/W4386715715","https://openalex.org/W4386923854","https://openalex.org/W4393044100","https://openalex.org/W4396670638","https://openalex.org/W4403820889","https://openalex.org/W4406067425","https://openalex.org/W4407261894","https://openalex.org/W4408233779","https://openalex.org/W4411609728","https://openalex.org/W4415816568","https://openalex.org/W4416673706"],"related_works":["https://openalex.org/W4415816568","https://openalex.org/W2029646215","https://openalex.org/W1966137693","https://openalex.org/W1429422322","https://openalex.org/W2133062309","https://openalex.org/W1542991620","https://openalex.org/W1991284709","https://openalex.org/W2064394850","https://openalex.org/W7100336434","https://openalex.org/W2087969314"],"abstract_inverted_index":{"In":[0],"vitro":[1],"regeneration":[2,143,165,192,217,234],"remains":[3],"a":[4,19,232],"major":[5],"bottleneck":[6],"for":[7,23],"the":[8,119,206,221],"genetic":[9,240],"improvement":[10],"of":[11,174,210,216],"recalcitrant":[12,199,226],"grain":[13,200,227],"legumes.":[14,201,228],"The":[15,141,164,229],"present":[16],"study":[17],"evaluated":[18],"TDZ-mediated":[20],"flooding":[21,108,188,222],"approach":[22,223],"rapid":[24],"and":[25,46,56,80,87,99,127,138,160,172,193,208,219,242],"high-frequency":[26],"apparent":[27],"direct":[28],"shoot":[29,91,105,191],"regeneration,":[30],"with":[31,96,109,114,169,178,236],"no":[32,90,179],"visible":[33,180],"callus":[34,181],"formation":[35,173],"based":[36],"on":[37,62],"gross":[38],"morphological":[39],"observations,":[40],"in":[41,73,77,82,130,146,239],"Cicer":[42],"arietinum":[43],"(black":[44],"gram":[45,79,158,162],"white":[47,78,133,161],"gram),":[48],"Glycine":[49],"max":[50],"(soybean),":[51],"Phaseolus":[52],"vulgaris":[53],"(common":[54],"bean),":[55],"Vigna":[57],"unguiculata":[58],"(cowpea).":[59],"Seedlings":[60],"raised":[61],"N":[63,110],"6":[64,111],"medium":[65,112],"containing":[66],"1.0":[67,115],"\u00b5M":[68,116],"TDZ":[69,117,187,211],"produced":[70,118],"4.5":[71],"shoots/seedling":[72,129],"black":[74,131,157],"gram,":[75,132,134],"2.45":[76],"1.9":[81],"common":[83,136,154],"bean,":[84],"while":[85],"soybean":[86,152],"cowpea":[88,147],"showed":[89],"regeneration.":[92],"Flooding":[93],"TDZ-raised":[94],"seedlings":[95],"distilled":[97],"water":[98],"sucrose":[100],"(D.W":[101],"+":[102],"S)":[103],"enhanced":[104],"formation,":[106],"whereas":[107],"supplemented":[113],"strongest":[120],"response,":[121],"yielding":[122],"17.7,":[123],"16.4,":[124],"24,":[125],"23":[126],"41":[128],"soybean,":[135],"bean":[137,155],"cowpea,":[139],"respectively.":[140],"maximum":[142],"was":[144,167],"recorded":[145],"(41":[148],"shoots/seedling),":[149],"followed":[150],"by":[151],"(24),":[153],"(23),":[156],"(17.7)":[159],"(16.4).":[163],"response":[166],"associated":[168],"shoot-apex":[170],"expansion":[171],"additional":[175,198,225],"shoot-forming":[176],"regions,":[177],"observed.":[182],"These":[183,202],"findings":[184,203],"demonstrate":[185,204],"that":[186,205],"substantially":[189],"enhances":[190],"extends":[194],"its":[195],"application":[196],"to":[197,224],"timing":[207],"mode":[209],"delivery":[212],"are":[213],"critical":[214],"determinants":[215],"efficiency":[218],"extend":[220],"protocol":[230],"provides":[231],"simple,":[233],"platform":[235],"potential":[237],"applications":[238],"transformation":[241],"genome":[243],"editing.":[244]},"counts_by_year":[],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2026-11-06T00:00:00"}