{"id":"https://openalex.org/W7225335121","doi":"https://doi.org/10.1136/jitc-2026-sitc2026.0059","title":"59 Charting the murine immune landscape: high-plex spatial profiling across diverse tissues","display_name":"59 Charting the murine immune landscape: high-plex spatial profiling across diverse tissues","publication_year":2026,"publication_date":"2026-11-01","ids":{"openalex":"https://openalex.org/W7225335121","doi":"https://doi.org/10.1136/jitc-2026-sitc2026.0059"},"language":"en","primary_location":{"id":"doi:10.1136/jitc-2026-sitc2026.0059","doi":"https://doi.org/10.1136/jitc-2026-sitc2026.0059","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2026-sitc2026.0059","pdf_url":"https://jitc.bmj.com/content/14/Suppl_2/A65.full.pdf","source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular Abstracts","raw_type":"proceedings-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://jitc.bmj.com/content/14/Suppl_2/A65.full.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081517023","display_name":"Danielle M Fails","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Danielle M Fails","raw_affiliation_strings":["Fortis Life Sciences, Montgomery, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fortis Life Sciences, Montgomery, TX, USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5021207450","display_name":"Alyssa Hernandez","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Alyssa Hernandez","raw_affiliation_strings":["Fortis Life Sciences, Montgomery, TX, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fortis Life Sciences, Montgomery, TX, USA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":2490,"currency":"USD","value_usd":2490},"apc_paid":{"value":2490,"currency":"USD","value_usd":2490},"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"A65","last_page":"A65"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.4426000118255615,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.4426000118255615,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.3571999967098236,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.05040000006556511,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/multiplex-immunofluorescence","display_name":"multiplex immunofluorescence","score":0.7269999980926514},{"id":"https://openalex.org/keywords/spatial-immune-profiling","display_name":"spatial immune profiling","score":0.7689999938011169},{"id":"https://openalex.org/keywords/automated-staining","display_name":"automated staining","score":0.37599998712539673},{"id":"https://openalex.org/keywords/immune-checkpoint-proteins","display_name":"immune checkpoint molecules","score":0.5860000252723694},{"id":"https://openalex.org/keywords/pd-1","display_name":"PD-1","score":0.7760000228881836},{"id":"https://openalex.org/keywords/lag-3","display_name":"LAG-3","score":0.9860000014305115},{"id":"https://openalex.org/keywords/t-cell-subsets","display_name":"T cell subsets","score":0.7319999933242798},{"id":"https://openalex.org/keywords/b-cells","display_name":"B cells","score":0.8349999785423279},{"id":"https://openalex.org/keywords/macrophages","display_name":"macrophages","score":0.9769999980926514},{"id":"https://openalex.org/keywords/dendritic-cells","display_name":"dendritic cells","score":0.996999979019165},{"id":"https://openalex.org/keywords/signal-amplification","display_name":"signal amplification","score":0.578000009059906},{"id":"https://openalex.org/keywords/spleen","display_name":"spleen","score":0.6269999742507935},{"id":"https://openalex.org/keywords/lymph-nodes","display_name":"lymph nodes","score":0.9850000143051147}],"concepts":[{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.7542999982833862},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.6252999901771545},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5475000143051147},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.5443999767303467},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5085999965667725},{"id":"https://openalex.org/C125473707","wikidata":"https://www.wikidata.org/wiki/Q9914","display_name":"H&E stain","level":3,"score":0.4896000027656555},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.4645000100135803},{"id":"https://openalex.org/C2778236600","wikidata":"https://www.wikidata.org/wiki/Q592324","display_name":"Immunofluorescence","level":3,"score":0.46160000562667847},{"id":"https://openalex.org/C21269302","wikidata":"https://www.wikidata.org/wiki/Q7243098","display_name":"Primary and secondary antibodies","level":3,"score":0.4505000114440918},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.4438000023365021},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.40959998965263367},{"id":"https://openalex.org/C2780849966","wikidata":"https://www.wikidata.org/wiki/Q170758","display_name":"Lymph node","level":2,"score":0.38670000433921814},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.3368000090122223},{"id":"https://openalex.org/C2778015335","wikidata":"https://www.wikidata.org/wiki/Q17051163","display_name":"Mass cytometry","level":4,"score":0.31869998574256897},{"id":"https://openalex.org/C2780339063","wikidata":"https://www.wikidata.org/wiki/Q5201367","display_name":"Cytometry","level":3,"score":0.31839999556541443},{"id":"https://openalex.org/C153083717","wikidata":"https://www.wikidata.org/wiki/Q6535263","display_name":"Leverage (statistics)","level":2,"score":0.31779998540878296},{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.30469998717308044},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.29750001430511475},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.29159998893737793},{"id":"https://openalex.org/C2776950831","wikidata":"https://www.wikidata.org/wiki/Q15635432","display_name":"Single-cell analysis","level":3,"score":0.2912999987602234},{"id":"https://openalex.org/C196166836","wikidata":"https://www.wikidata.org/wiki/Q902926","display_name":"Immunophenotyping","level":3,"score":0.27219998836517334},{"id":"https://openalex.org/C63479239","wikidata":"https://www.wikidata.org/wiki/Q7353546","display_name":"Robustness (evolution)","level":3,"score":0.2667999863624573},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.2623000144958496},{"id":"https://openalex.org/C2779751288","wikidata":"https://www.wikidata.org/wiki/Q422637","display_name":"Eosin","level":3,"score":0.257099986076355},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.2549000084400177}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1136/jitc-2026-sitc2026.0059","doi":"https://doi.org/10.1136/jitc-2026-sitc2026.0059","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2026-sitc2026.0059","pdf_url":"https://jitc.bmj.com/content/14/Suppl_2/A65.full.pdf","source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular Abstracts","raw_type":"proceedings-article"}],"best_oa_location":{"id":"doi:10.1136/jitc-2026-sitc2026.0059","doi":"https://doi.org/10.1136/jitc-2026-sitc2026.0059","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2026-sitc2026.0059","pdf_url":"https://jitc.bmj.com/content/14/Suppl_2/A65.full.pdf","source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular Abstracts","raw_type":"proceedings-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225335121.pdf","grobid_xml":"https://content.openalex.org/works/W7225335121.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4416449627","https://openalex.org/W4311326648","https://openalex.org/W7159665025","https://openalex.org/W4362592658","https://openalex.org/W4393087885","https://openalex.org/W2982547352","https://openalex.org/W4388048553","https://openalex.org/W4404064951","https://openalex.org/W4393095681","https://openalex.org/W7171534029"],"abstract_inverted_index":{"Background":[0],"The":[1,343],"immune":[2,51,85,277,341,386,409],"system":[3],"is":[4,420,439],"a":[5,45,80,327,380],"highly":[6,196,331],"dynamic":[7],"network":[8],"whose":[9],"function":[10],"depends":[11],"on":[12,191],"the":[13,48,132,155,172,192,243,247,298,323,339,346,351,364,408,443],"precise":[14],"spatial":[15,39,336],"organization":[16],"and":[17,67,109,116,152,160,180,199,281,306,330,353,393,431,452],"interaction":[18],"of":[19,83,187,300,338,345,350,366,390,436,449],"heterogeneous":[20],"cell":[21,102],"populations":[22],"within":[23],"native":[24],"tissue":[25,65,111,183,253,361,418],"architectures.":[26],"1":[27],"Traditional":[28],"Methods":[29],"like":[30],"flow":[31],"cytometry":[32],"capture":[33],"cellular":[34],"abundance":[35],"but":[36,446],"lose":[37],"critical":[38],"context.":[40],"While":[41],"multiplex":[42],"immunofluorescence":[43,268],"provides":[44,326],"solution,":[46],"characterizing":[47],"complex":[49],"murine":[50,84,340],"landscape":[52],"across":[53,118,204,238],"multiple":[54],"distinct":[55,447],"organs":[56],"has":[57],"historically":[58],"been":[59],"hindered":[60],"by":[61,78],"long":[62],"optimization":[63,262],"timelines,":[64],"degradation,":[66],"rigid":[68],"antibody":[69,218,367],"requirements.":[70],"This":[71,375],"study":[72],"aimed":[73],"to":[74,87,165,265,441],"address":[75],"these":[76],"constraints":[77],"developing":[79],"high-plex":[81,202,348],"panel":[82,98,133,190],"markers":[86,112],"use":[88],"in":[89,131,255,320,388,407],"combination":[90,321],"with":[91,178,233,322,422],"automated":[92,173,201,244],"staining":[93,159,203,240,271,355],"technology.Methods":[94],"A":[95,434],"comprehensive":[96],"16-marker":[97,189,249],"targeting":[99],"key":[100],"T":[101],"subsets,":[103],"B":[104],"cells,":[105,108],"macrophages,":[106],"dendritic":[107],"structural":[110,283,308],"was":[113],"developed,":[114],"optimized,":[115],"run":[117,245],"diverse":[119],"FFPE":[120],"mouse":[121,207],"tissues":[122],"(i.e.,":[123],"spleen,":[124],"lymph":[125],"nodes,":[126],"lung,":[127],"gut).":[128],"Biomarkers":[129],"included":[130],"were":[134,176,273],"as":[135,379],"follows:":[136],"MEF2A,":[137],"F4/80,":[138],"aSMA,":[139],"CD31,":[140],"CD45,":[141],"Tim-3,":[142],"CD4,":[143],"CD3,":[144],"CD20,":[145],"FoxP3,":[146],"EGFR,":[147],"Lag-3,":[148],"COL1A1,":[149],"COL4A1,":[150],"PD-1,":[151],"CD8.":[153],"Utilizing":[154],"Lunaphore":[156],"COMET\u2122":[157,193,324],"integrated":[158],"imaging":[161],"platform":[162,194,325],"allowed":[163],"us":[164],"leverage":[166],"off-the-shelf,":[167],"un-conjugated":[168],"primary":[169,212],"antibodies.":[170],"Following":[171],"run,":[174],"samples":[175,254,301],"counterstained":[177],"hematoxylin":[179],"eosin":[181],"for":[182,225,251,275,334,358,383],"architecture":[184,309],"visualization.Results":[185],"Validation":[186],"this":[188,317],"demonstrated":[195],"robust,":[197,329],"reproducible,":[198],"fully":[200],"all":[205,226,252],"tested":[206],"tissues.":[208],"By":[209],"utilizing":[210],"off-the-shelf":[211],"antibodies,":[213],"we":[214],"bypassed":[215],"traditional":[216],"secondary":[217],"species":[219],"crosstalk,":[220],"enabling":[221],"uniform":[222],"signal":[223,236],"amplification":[224],"markers.":[227],"Signal-to-noise":[228],"ratios":[229],"remained":[230],"consistently":[231],"high,":[232],"no":[234,303],"observable":[235],"dimming":[237],"sequential":[239,289],"cycles.":[241],"Additionally,":[242,363],"completed":[246],"entire":[248],"sequence":[250],"under":[256],"36":[257],"hours,":[258],"significantly":[259,371],"reducing":[260],"hands-on":[261],"time":[263],"compared":[264],"manual":[266],"cyclic":[267],"protocols.":[269],"Concordant":[270],"patterns":[272],"achieved":[274],"low-abundance":[276],"checkpoints":[278],"(PD-1,":[279],"Lag-3)":[280],"high-density":[282],"targets":[284],"(COL4A1,":[285],"aSMA),":[286],"confirming":[287],"that":[288,316],"elution":[290],"cycles":[291],"did":[292],"not":[293],"compromise":[294],"epitope":[295],"availability.":[296],"Finally,":[297],"counterstaining":[299],"revealed":[302],"artifact":[304],"introduction":[305],"preserved":[307],"(":[310],"figure":[311],"1).Conclusions":[312],"Our":[313],"findings":[314],"demonstrate":[315],"16-plex":[318],"assay":[319,373],"rapid,":[328],"flexible":[332],"workflow":[333,376],"broad":[335],"profiling":[337],"system.":[342,410],"automation":[344],"instrument,":[347],"nature":[349],"assay,":[352],"gentle":[354],"procedure":[356],"allow":[357],"additional":[359],"downstream":[360],"use.":[362],"lack":[365],"barcoding":[368],"or":[369],"conjugation":[370],"accelerated":[372],"development.":[374],"could":[377],"serve":[378],"valuable":[381],"pipeline":[382],"evaluating":[384],"tissue-specific":[385],"responses":[387],"models":[389],"infection,":[391],"inflammation,":[392],"cancer":[394],"immunotherapy.Reference":[395],"Navarro":[396],"QR,":[397],"Villarreal":[398],"CJ,":[399],"Zarate":[400],"PE,":[401],"et":[402],"al.":[403],"Multiscale":[404],"information":[405],"processing":[406],"Front":[411],"Immunol.":[412],"2025;16:1563992.Abstract":[413],"59":[414],"Figure":[415],"1Mouse":[416],"spleen":[417],"(A)":[419],"stained":[421],"MEF2A":[423,453],"(red),":[424],"F4/80":[425,451],"(green),":[426],"CD31":[427],"(white),":[428],"aSMA":[429],"(yellow),":[430],"DAPI":[432],"(blue).":[433],"region":[435],"interest":[437],"(B)":[438],"chosen":[440],"display":[442],"high":[444],"density,":[445],"populations,":[448],"positive":[450],"cells":[454]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-11-06T00:00:00"}
