{"id":"https://openalex.org/W7225320219","doi":"https://doi.org/10.1021/acs.jafc.6c06547","title":"Metabolic Engineering of Pichia pastoris for De Novo Biosynthesis of D-Lactic Acid from Methanol","display_name":"Metabolic Engineering of Pichia pastoris for De Novo Biosynthesis of D-Lactic Acid from Methanol","publication_year":2026,"publication_date":"2026-11-05","ids":{"openalex":"https://openalex.org/W7225320219","doi":"https://doi.org/10.1021/acs.jafc.6c06547"},"language":"en","primary_location":{"id":"doi:10.1021/acs.jafc.6c06547","doi":"https://doi.org/10.1021/acs.jafc.6c06547","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.jafc.6c06547","pdf_url":null,"source":{"id":"https://openalex.org/S134644764","display_name":"Journal of Agricultural and Food Chemistry","issn_l":"0021-8561","issn":["0021-8561","1520-5118"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Agricultural and Food Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5160756033","display_name":"Chenmeng Zhang","orcid":"https://orcid.org/0000-0002-0559-1749","observed_orcids":["https://orcid.org/0000-0002-0559-1749"]},"institutions":[{"id":"https://openalex.org/I167027274","display_name":"Nanjing Forestry University","ror":"https://ror.org/03m96p165","country_code":"CN","type":"education","lineage":["https://openalex.org/I167027274"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chenmeng Zhang","raw_affiliation_strings":["Nanjing Forestry University , , ,"],"raw_orcid":"https://orcid.org/0000-0002-0559-1749","affiliations":[{"raw_affiliation_string":"Nanjing Forestry University , , ,","institution_ids":["https://openalex.org/I167027274"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5148930318","display_name":"Yuncong Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I167027274","display_name":"Nanjing Forestry University","ror":"https://ror.org/03m96p165","country_code":"CN","type":"education","lineage":["https://openalex.org/I167027274"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yuncong Wang","raw_affiliation_strings":["Nanjing Forestry University , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Nanjing Forestry University , , ,","institution_ids":["https://openalex.org/I167027274"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160941891","display_name":"Xun Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I167027274","display_name":"Nanjing Forestry University","ror":"https://ror.org/03m96p165","country_code":"CN","type":"education","lineage":["https://openalex.org/I167027274"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xun Wang","raw_affiliation_strings":["Nanjing Forestry University , , ,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Nanjing Forestry University , , ,","institution_ids":["https://openalex.org/I167027274"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063077523","display_name":"Yu Zhang","orcid":"https://orcid.org/0000-0002-2614-5975","observed_orcids":["https://orcid.org/0000-0002-2614-5975"]},"institutions":[{"id":"https://openalex.org/I167027274","display_name":"Nanjing Forestry University","ror":"https://ror.org/03m96p165","country_code":"CN","type":"education","lineage":["https://openalex.org/I167027274"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yu Zhang","raw_affiliation_strings":["Nanjing Forestry University , , ,"],"raw_orcid":"https://orcid.org/0000-0002-2614-5975","affiliations":[{"raw_affiliation_string":"Nanjing Forestry University , , ,","institution_ids":["https://openalex.org/I167027274"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100341846","display_name":"Xun Li","orcid":"https://orcid.org/0000-0002-1267-9263","observed_orcids":["https://orcid.org/0000-0002-1267-9263"]},"institutions":[{"id":"https://openalex.org/I167027274","display_name":"Nanjing Forestry University","ror":"https://ror.org/03m96p165","country_code":"CN","type":"education","lineage":["https://openalex.org/I167027274"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Xun Li","raw_affiliation_strings":["Nanjing Forestry University , , ,"],"raw_orcid":"https://orcid.org/0000-0002-1267-9263","affiliations":[{"raw_affiliation_string":"Nanjing Forestry University , , ,","institution_ids":["https://openalex.org/I167027274"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100455718","display_name":"Fei Wang","orcid":"https://orcid.org/0000-0001-6428-5111","observed_orcids":["https://orcid.org/0000-0001-6428-5111","https://orcid.org/0000-0001-7949-6114"]},"institutions":[{"id":"https://openalex.org/I167027274","display_name":"Nanjing Forestry University","ror":"https://ror.org/03m96p165","country_code":"CN","type":"education","lineage":["https://openalex.org/I167027274"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Fei Wang","raw_affiliation_strings":["Nanjing Forestry University , , ,"],"raw_orcid":"https://orcid.org/0000-0001-6428-5111","affiliations":[{"raw_affiliation_string":"Nanjing Forestry University , , ,","institution_ids":["https://openalex.org/I167027274"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5100341846","https://openalex.org/A5100455718"],"corresponding_institution_ids":["https://openalex.org/I167027274"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.003599999938160181,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10661","display_name":"biodegradable polymer synthesis and properties","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/2502","display_name":"Biomaterials"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/d-lactate","display_name":"D-lactate","score":0.9520000219345093},{"id":"https://openalex.org/keywords/komagataella-phaffii","display_name":"Pichia pastoris","score":0.9769999980926514},{"id":"https://openalex.org/keywords/de-novo-synthesis","display_name":"de novo synthesis","score":0.7870000004768372},{"id":"https://openalex.org/keywords/c1-feedstock","display_name":"C1 feedstock","score":0.6499999761581421},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"metabolic engineering","score":0.7839999794960022},{"id":"https://openalex.org/keywords/acid-tolerance","display_name":"acid resistance","score":0.5830000042915344},{"id":"https://openalex.org/keywords/sodium-citrate","display_name":"sodium citrate","score":0.7630000114440918},{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.9549999833106995},{"id":"https://openalex.org/keywords/biobased-monomers","display_name":"bio-based monomers","score":0.7120000123977661}],"concepts":[{"id":"https://openalex.org/C2781372317","wikidata":"https://www.wikidata.org/wiki/Q148697","display_name":"Pichia pastoris","level":4,"score":0.9351999759674072},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.8073999881744385},{"id":"https://openalex.org/C2780074830","wikidata":"https://www.wikidata.org/wiki/Q864423","display_name":"Bioconversion","level":3,"score":0.7523999810218811},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.6146000027656555},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.605400025844574},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5856999754905701},{"id":"https://openalex.org/C2779607525","wikidata":"https://www.wikidata.org/wiki/Q14982","display_name":"Methanol","level":2,"score":0.5454999804496765},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.39629998803138733},{"id":"https://openalex.org/C4916135","wikidata":"https://www.wikidata.org/wiki/Q4119055","display_name":"Bioprocess","level":2,"score":0.36970001459121704},{"id":"https://openalex.org/C513059894","wikidata":"https://www.wikidata.org/wiki/Q540912","display_name":"Food industry","level":2,"score":0.3612000048160553},{"id":"https://openalex.org/C143517461","wikidata":"https://www.wikidata.org/wiki/Q1138571","display_name":"Renewable resource","level":3,"score":0.3598000109195709},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.352400004863739},{"id":"https://openalex.org/C192989942","wikidata":"https://www.wikidata.org/wiki/Q68685","display_name":"Metabolic pathway","level":3,"score":0.3521000146865845},{"id":"https://openalex.org/C2781162966","wikidata":"https://www.wikidata.org/wiki/Q413769","display_name":"Polylactic acid","level":3,"score":0.349700003862381},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.34540000557899475},{"id":"https://openalex.org/C61664519","wikidata":"https://www.wikidata.org/wiki/Q864734","display_name":"Bioprocess engineering","level":2,"score":0.3368000090122223},{"id":"https://openalex.org/C188573790","wikidata":"https://www.wikidata.org/wiki/Q12705","display_name":"Renewable energy","level":2,"score":0.33250001072883606},{"id":"https://openalex.org/C2779703480","wikidata":"https://www.wikidata.org/wiki/Q125153","display_name":"Pichia","level":5,"score":0.3303999900817871},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.32670000195503235},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.3255000114440918},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.31949999928474426},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.29019999504089355},{"id":"https://openalex.org/C166940927","wikidata":"https://www.wikidata.org/wiki/Q178827","display_name":"Monomer","level":3,"score":0.29019999504089355},{"id":"https://openalex.org/C543025807","wikidata":"https://www.wikidata.org/wiki/Q61476","display_name":"Fatty acid","level":2,"score":0.289900004863739},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.288100004196167},{"id":"https://openalex.org/C98045186","wikidata":"https://www.wikidata.org/wiki/Q205663","display_name":"Process (computing)","level":2,"score":0.2858999967575073},{"id":"https://openalex.org/C2778236407","wikidata":"https://www.wikidata.org/wiki/Q1041116","display_name":"Generally recognized as safe","level":2,"score":0.2786000072956085},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.2700999975204468},{"id":"https://openalex.org/C53105671","wikidata":"https://www.wikidata.org/wiki/Q14777816","display_name":"Rational design","level":2,"score":0.2615000009536743},{"id":"https://openalex.org/C2994087729","wikidata":"https://www.wikidata.org/wiki/Q7649686","display_name":"Sustainable production","level":3,"score":0.26019999384880066},{"id":"https://openalex.org/C91558341","wikidata":"https://www.wikidata.org/wiki/Q725361","display_name":"Green chemistry","level":4,"score":0.25519999861717224}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1021/acs.jafc.6c06547","doi":"https://doi.org/10.1021/acs.jafc.6c06547","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.jafc.6c06547","pdf_url":null,"source":{"id":"https://openalex.org/S134644764","display_name":"Journal of Agricultural and Food Chemistry","issn_l":"0021-8561","issn":["0021-8561","1520-5118"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Agricultural and Food Chemistry","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.807699978351593,"display_name":"Responsible consumption and production","id":"https://metadata.un.org/sdg/12"},{"score":0.75,"display_name":"Affordable and clean energy","id":"https://metadata.un.org/sdg/7"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320321543","display_name":"China Postdoctoral Science Foundation","ror":"https://ror.org/0426zh255"},{"id":"https://openalex.org/F4320322769","display_name":"Natural Science Foundation of Jiangsu Province","ror":"https://ror.org/01h0zpd94"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W1873692790","https://openalex.org/W1968652158","https://openalex.org/W2060597438","https://openalex.org/W2069479491","https://openalex.org/W2095668363","https://openalex.org/W2105248706","https://openalex.org/W2133071016","https://openalex.org/W2143218252","https://openalex.org/W2179997010","https://openalex.org/W2195560313","https://openalex.org/W2282698399","https://openalex.org/W2339999858","https://openalex.org/W2568012904","https://openalex.org/W2591922605","https://openalex.org/W2595491014","https://openalex.org/W2741598729","https://openalex.org/W2890950898","https://openalex.org/W2913026913","https://openalex.org/W2917746550","https://openalex.org/W2978882869","https://openalex.org/W3132973701","https://openalex.org/W3173387900","https://openalex.org/W4251024958","https://openalex.org/W4285008791","https://openalex.org/W4310172150","https://openalex.org/W4316363203","https://openalex.org/W4361278087","https://openalex.org/W4363677993","https://openalex.org/W4382862288","https://openalex.org/W4396688534","https://openalex.org/W4401422564","https://openalex.org/W4403476280","https://openalex.org/W4403936312","https://openalex.org/W4408344855","https://openalex.org/W4415096932","https://openalex.org/W4416717077","https://openalex.org/W4416794254","https://openalex.org/W7140129841"],"related_works":["https://openalex.org/W4413037127","https://openalex.org/W4403936312","https://openalex.org/W2913026913","https://openalex.org/W4411866651","https://openalex.org/W7161126312","https://openalex.org/W7148630882","https://openalex.org/W4213095427","https://openalex.org/W7161054876","https://openalex.org/W2212869455","https://openalex.org/W4391220089"],"abstract_inverted_index":{"Abstract":[0],"D-Lactic":[1],"acid":[2,11,83],"(D-LA)":[3],"is":[4,25],"an":[5,70,105],"essential":[6],"chiral":[7],"monomer":[8],"for":[9,38,51,125,136],"polylactic":[10],"(PLA),":[12],"widely":[13],"used":[14],"in":[15],"biodegradable":[16],"food":[17,30],"packaging":[18],"and":[19,32,44,85,113,128,140],"agricultural":[20],"materials.":[21],"Conventional":[22],"sugar-based":[23],"fermentation":[24],"constrained":[26],"by":[27,64],"competition":[28],"with":[29],"resources":[31],"price":[33],"volatility,":[34],"highlighting":[35],"the":[36,89],"need":[37],"sustainable":[39,123],"alternatives.":[40],"Methanol,":[41],"a":[42,94,122,133],"renewable":[43,144],"cost-effective":[45],"C1":[46,145],"substrate,":[47],"provides":[48],"thermodynamic":[49],"advantages":[50],"D-LA":[52,77,95,126],"biosynthesis":[53],"but":[54],"remains":[55],"underutilized.":[56],"In":[57],"this":[58],"study,":[59],"Pichia":[60],"pastoris":[61,131],"was":[62,102],"modified":[63],"systematic":[65],"metabolic":[66,107],"engineering":[67],"to":[68,109],"create":[69],"efficient":[71],"methanol-D-LA":[72],"conversion":[73],"pathway.":[74],"By":[75],"reducing":[76],"degradation,":[78],"enhancing":[79],"methanol":[80,119],"utilization,":[81],"improving":[82],"tolerance,":[84],"eliminating":[86],"competing":[87],"pathways,":[88],"optimized":[90],"fed-batch":[91],"process":[92,114],"achieved":[93],"titer":[96],"of":[97],"12.05":[98],"g/L.":[99],"Sodium":[100],"citrate":[101],"employed":[103],"as":[104,121,132],"auxiliary":[106],"regulator":[108],"support":[110],"central":[111],"metabolism":[112],"robustness.":[115],"This":[116],"work":[117],"demonstrates":[118],"bioconversion":[120],"route":[124],"production":[127],"establishes":[129],"P.":[130],"promising":[134],"platform":[135],"manufacturing":[137],"biobased":[138],"monomers":[139],"high-value":[141],"chemicals":[142],"from":[143],"feedstocks.":[146]},"counts_by_year":[],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2026-11-06T00:00:00"}