{"id":"https://openalex.org/W7225309934","doi":"https://doi.org/10.6084/m9.figshare.34920324","title":"Tracing the Evolutionary Histories of Human Cholinergic Proteins Across Eukaryotes: A Comparative Phylogenomic Study","display_name":"Tracing the Evolutionary Histories of Human Cholinergic Proteins Across Eukaryotes: A Comparative Phylogenomic Study","publication_year":2026,"publication_date":"2026-11-05","ids":{"openalex":"https://openalex.org/W7225309934","doi":"https://doi.org/10.6084/m9.figshare.34920324"},"language":"en","primary_location":{"id":"doi:10.6084/m9.figshare.34920324","doi":"https://doi.org/10.6084/m9.figshare.34920324","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.34920324","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Preprint"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.6084/m9.figshare.34920324","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5126401440","display_name":"Ankit Thakur","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ankit Thakur","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5084759137","display_name":"Mahesh Kulharia","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mahesh Kulharia","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9345999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9345999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11818","display_name":"Nicotinic Acetylcholine Receptors Study","score":0.007499999832361937,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.007400000002235174,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cholinergic-system","display_name":"cholinergic system","score":0.7279999852180481},{"id":"https://openalex.org/keywords/protein-evolution","display_name":"protein evolution","score":0.7329999804496765},{"id":"https://openalex.org/keywords/eukaryotic-phylogeny","display_name":"eukaryotic phylogeny","score":0.7099999785423279},{"id":"https://openalex.org/keywords/choline-acetyltransferase","display_name":"choline acetyltransferase","score":0.7580000162124634},{"id":"https://openalex.org/keywords/slc5a7","display_name":"SLC5A7","score":0.7760000228881836},{"id":"https://openalex.org/keywords/cholinesterase","display_name":"cholinesterase","score":0.8429999947547913},{"id":"https://openalex.org/keywords/acetylcholinesterase","display_name":"acetylcholinesterase","score":0.9089999794960022},{"id":"https://openalex.org/keywords/butyrylcholinesterase","display_name":"butyrylcholinesterase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/muscarinic-receptors","display_name":"muscarinic receptors","score":0.9679999947547913},{"id":"https://openalex.org/keywords/nicotinic-acetylcholine-receptors","display_name":"nicotinic acetylcholine receptors","score":0.9810000061988831},{"id":"https://openalex.org/keywords/gene-duplication-and-loss","display_name":"gene duplication and loss","score":0.8690000176429749},{"id":"https://openalex.org/keywords/orthology","display_name":"orthology","score":0.6740000247955322},{"id":"https://openalex.org/keywords/gene-tree-species-tree-reconciliation","display_name":"gene tree-species tree reconciliation","score":0.6600000262260437},{"id":"https://openalex.org/keywords/ctenophores","display_name":"ctenophores","score":0.7990000247955322},{"id":"https://openalex.org/keywords/cnidaria","display_name":"Cnidaria","score":0.9350000023841858},{"id":"https://openalex.org/keywords/bilaterians","display_name":"bilaterians","score":0.7749999761581421},{"id":"https://openalex.org/keywords/echinoderms","display_name":"Echinodermata","score":0.9350000023841858}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8095999956130981},{"id":"https://openalex.org/C193252679","wikidata":"https://www.wikidata.org/wiki/Q242125","display_name":"Phylogenetic tree","level":3,"score":0.7461000084877014},{"id":"https://openalex.org/C90132467","wikidata":"https://www.wikidata.org/wiki/Q171184","display_name":"Phylogenetics","level":3,"score":0.6815000176429749},{"id":"https://openalex.org/C2779343474","wikidata":"https://www.wikidata.org/wiki/Q3109175","display_name":"Context (archaeology)","level":2,"score":0.6187999844551086},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.5911999940872192},{"id":"https://openalex.org/C44465124","wikidata":"https://www.wikidata.org/wiki/Q713623","display_name":"Clade","level":4,"score":0.52920001745224},{"id":"https://openalex.org/C70343354","wikidata":"https://www.wikidata.org/wiki/Q3381973","display_name":"Phylogenomics","level":5,"score":0.43779999017715454},{"id":"https://openalex.org/C62142553","wikidata":"https://www.wikidata.org/wiki/Q200312","display_name":"Convergent evolution","level":4,"score":0.4180999994277954},{"id":"https://openalex.org/C7602840","wikidata":"https://www.wikidata.org/wiki/Q746284","display_name":"Gene duplication","level":3,"score":0.3806000053882599},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.36649999022483826},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.35989999771118164},{"id":"https://openalex.org/C144292202","wikidata":"https://www.wikidata.org/wiki/Q898273","display_name":"Protein domain","level":3,"score":0.35359999537467957},{"id":"https://openalex.org/C166393388","wikidata":"https://www.wikidata.org/wiki/Q856529","display_name":"Molecular evolution","level":4,"score":0.3483999967575073},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3312000036239624},{"id":"https://openalex.org/C92938381","wikidata":"https://www.wikidata.org/wiki/Q83185","display_name":"Horizontal gene transfer","level":4,"score":0.31360000371932983},{"id":"https://openalex.org/C10010492","wikidata":"https://www.wikidata.org/wiki/Q3142557","display_name":"Protein sequencing","level":4,"score":0.31349998712539673},{"id":"https://openalex.org/C199216141","wikidata":"https://www.wikidata.org/wiki/Q4995178","display_name":"Conserved sequence","level":4,"score":0.3086000084877014},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.29989999532699585},{"id":"https://openalex.org/C189592816","wikidata":"https://www.wikidata.org/wiki/Q427626","display_name":"Taxonomic rank","level":3,"score":0.2897999882698059},{"id":"https://openalex.org/C2778961482","wikidata":"https://www.wikidata.org/wiki/Q25241","display_name":"Vertebrate","level":3,"score":0.2892000079154968},{"id":"https://openalex.org/C188987157","wikidata":"https://www.wikidata.org/wiki/Q7030581","display_name":"Nicotinic agonist","level":3,"score":0.2761000096797943},{"id":"https://openalex.org/C22885893","wikidata":"https://www.wikidata.org/wiki/Q4862743","display_name":"Cholinergic","level":2,"score":0.2669000029563904},{"id":"https://openalex.org/C45484198","wikidata":"https://www.wikidata.org/wiki/Q827246","display_name":"Sequence alignment","level":4,"score":0.2646999955177307},{"id":"https://openalex.org/C194167682","wikidata":"https://www.wikidata.org/wiki/Q4787088","display_name":"Architecture domain","level":5,"score":0.26100000739097595}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.6084/m9.figshare.34920324","doi":"https://doi.org/10.6084/m9.figshare.34920324","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.34920324","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Preprint"},{"id":"doi:10.6084/m9.figshare.34920324.v1","doi":"https://doi.org/10.6084/m9.figshare.34920324.v1","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.34920324.v1","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Preprint"}],"best_oa_location":{"id":"doi:10.6084/m9.figshare.34920324","doi":"https://doi.org/10.6084/m9.figshare.34920324","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.34920324","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Preprint"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2127597491","https://openalex.org/W2054773429","https://openalex.org/W164896969","https://openalex.org/W2591874061","https://openalex.org/W1993243902","https://openalex.org/W2724190799","https://openalex.org/W2951527967","https://openalex.org/W3041177019","https://openalex.org/W2032859962","https://openalex.org/W3042001574"],"abstract_inverted_index":{"Acetylcholine":[0],"signalling":[1],"depends":[2],"on":[3],"a":[4,34,108,184],"coordinated":[5],"network":[6],"of":[7,205],"enzymes,":[8],"transporters,":[9],"and":[10,46,53,68,82,102,125,129,142,149,156,170,210],"receptors":[11],"whose":[12],"evolutionary":[13,135,161,197],"histories":[14,198],"need":[15],"not":[16],"be":[17,200],"congruent.":[18],"Here,":[19],"we":[20],"examined":[21],"26":[22],"curated":[23],"human":[24,117,178],"cholinergic":[25,179],"reference":[26],"proteins":[27,174],"across":[28,87],"89":[29],"selected":[30,62,147],"eukaryotic":[31],"proteomes":[32],"using":[33,48],"human-reference-anchored":[35],"phylogenomic":[36],"framework.":[37],"Candidate":[38],"sequences":[39,94],"were":[40,85],"identified":[41,113],"through":[42],"sequence":[43,157],"similarity":[44],"searches":[45],"evaluated":[47],"domain":[49],"architecture,":[50],"orthology":[51],"assessment,":[52],"family-level":[54],"phylogenetic":[55,171,211],"analyses.":[56],"Gene-tree/species-tree":[57],"reconciliation":[58],"was":[59,112],"performed":[60],"for":[61,187],"datasets":[63],"to":[64,116,176],"investigate":[65],"putative":[66],"duplication":[67],"loss":[69],"histories.":[70],"The":[71,181],"results":[72],"revealed":[73],"heterogeneous":[74,167],"patterns":[75],"among":[76,173],"the":[77,105,177,203],"investigated":[78],"protein":[79,190],"groups.":[80],"CHAT-":[81],"SLC18A3-associated":[83],"candidates":[84],"recovered":[86,121,146,196],"multiple":[88],"sampled":[89,99],"bilaterian":[90],"lineages,":[91],"whereas":[92],"SLC5-family-related":[93],"showed":[95],"broader":[96,160],"representation,":[97],"including":[98],"ctenophores,":[100],"cnidarians,":[101],"bilaterians.":[103],"Within":[104],"latter":[106],"group,":[107],"distinct":[109,126],"vertebrate-associated":[110],"subgroup":[111],"in":[114,202],"relation":[115],"SLC5A7.":[118],"Cholinesterase":[119],"analyses":[120,145],"an":[122],"echinoderm-associated":[123],"grouping":[124],"vertebrate":[127],"ACHE-":[128],"BCHE-associated":[130],"groups,":[131],"although":[132],"their":[133,195],"deeper":[134],"relationship":[136],"received":[137],"comparatively":[138],"lower":[139],"support.":[140],"Muscarinic":[141],"nicotinic":[143],"receptor":[144],"subtype-":[148],"subunit-associated":[150],"groupings,":[151],"but":[152],"limited":[153],"taxonomic":[154,168,208],"representation":[155],"instability":[158],"constrained":[159],"interpretation.":[162],"Collectively,":[163],"these":[164,189],"findings":[165],"reveal":[166],"distributions":[169],"relationships":[172],"corresponding":[175],"system.":[180],"study":[182],"provides":[183],"comparative":[185],"framework":[186],"examining":[188],"groups":[191],"while":[192],"emphasizing":[193],"that":[194],"must":[199],"interpreted":[201],"context":[204],"reference-sequence":[206],"selection,":[207],"sampling,":[209],"resolution.":[212]},"counts_by_year":[],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2026-11-06T00:00:00"}