{"id":"https://openalex.org/W7225268272","doi":"https://doi.org/10.1080/17576180.2026.2758060","title":"Human seminal fluid as a novel Forensic matrix for flualprazolam and \u03b1-hydroxy flualprazolam: development and validation of a sensitive SPE\u2013UHPLC\u2013MS/MS method and application to real samples","display_name":"Human seminal fluid as a novel Forensic matrix for flualprazolam and \u03b1-hydroxy flualprazolam: development and validation of a sensitive SPE\u2013UHPLC\u2013MS/MS method and application to real samples","publication_year":2026,"publication_date":"2026-11-03","ids":{"openalex":"https://openalex.org/W7225268272","doi":"https://doi.org/10.1080/17576180.2026.2758060","pmid":"https://pubmed.ncbi.nlm.nih.gov/42978305"},"language":"en","primary_location":{"id":"doi:10.1080/17576180.2026.2758060","doi":"https://doi.org/10.1080/17576180.2026.2758060","is_oa":false,"landing_page_url":"https://doi.org/10.1080/17576180.2026.2758060","pdf_url":null,"source":{"id":"https://openalex.org/S159955403","display_name":"Bioanalysis","issn_l":"1757-6180","issn":["1757-6180","1757-6199"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioanalysis","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113393446","display_name":"Nadeem Ul Hassan Khan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128276","display_name":"Sa\u011fl\u0131k Bilimleri \u00dcniversitesi","ror":"https://ror.org/03k7bde87","country_code":"TR","type":"education","lineage":["https://openalex.org/I4210128276"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Nadeem Ul Hassan Khan","raw_affiliation_strings":["University of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Health Sciences","institution_ids":["https://openalex.org/I4210128276"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160605216","display_name":"Ahsan Rathore","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128276","display_name":"Sa\u011fl\u0131k Bilimleri \u00dcniversitesi","ror":"https://ror.org/03k7bde87","country_code":"TR","type":"education","lineage":["https://openalex.org/I4210128276"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Ahsan Rathore","raw_affiliation_strings":["University of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Health Sciences","institution_ids":["https://openalex.org/I4210128276"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160648326","display_name":"Waheed Muhammad Imran","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128276","display_name":"Sa\u011fl\u0131k Bilimleri \u00dcniversitesi","ror":"https://ror.org/03k7bde87","country_code":"TR","type":"education","lineage":["https://openalex.org/I4210128276"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Waheed Muhammad Imran","raw_affiliation_strings":["University of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Health Sciences","institution_ids":["https://openalex.org/I4210128276"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031098700","display_name":"Nauman Mazhar","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nauman Mazhar","raw_affiliation_strings":["Punjab Institute of Mental Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Punjab Institute of Mental Health","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5157983305","display_name":"Shahid Nazir","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128276","display_name":"Sa\u011fl\u0131k Bilimleri \u00dcniversitesi","ror":"https://ror.org/03k7bde87","country_code":"TR","type":"education","lineage":["https://openalex.org/I4210128276"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Shahid Nazir","raw_affiliation_strings":["University of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Health Sciences","institution_ids":["https://openalex.org/I4210128276"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011920470","display_name":"Anam Munawar","orcid":"https://orcid.org/0000-0002-2296-2118","observed_orcids":["https://orcid.org/0000-0002-2296-2118"]},"institutions":[{"id":"https://openalex.org/I4210128276","display_name":"Sa\u011fl\u0131k Bilimleri \u00dcniversitesi","ror":"https://ror.org/03k7bde87","country_code":"TR","type":"education","lineage":["https://openalex.org/I4210128276"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Anam Munawar","raw_affiliation_strings":["University of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Health Sciences","institution_ids":["https://openalex.org/I4210128276"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077666664","display_name":"Rahat Abdul Rehman","orcid":"https://orcid.org/0000-0002-9390-2569","observed_orcids":["https://orcid.org/0000-0002-9390-2569"]},"institutions":[{"id":"https://openalex.org/I4210128276","display_name":"Sa\u011fl\u0131k Bilimleri \u00dcniversitesi","ror":"https://ror.org/03k7bde87","country_code":"TR","type":"education","lineage":["https://openalex.org/I4210128276"]}],"countries":["TR"],"is_corresponding":true,"raw_author_name":"Rahat Abdul Rehman","raw_affiliation_strings":["University of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Health Sciences","institution_ids":["https://openalex.org/I4210128276"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5082551108","display_name":"Allah Rakha","orcid":"https://orcid.org/0000-0001-9752-2785","observed_orcids":["https://orcid.org/0000-0001-9752-2785"]},"institutions":[{"id":"https://openalex.org/I4210128276","display_name":"Sa\u011fl\u0131k Bilimleri \u00dcniversitesi","ror":"https://ror.org/03k7bde87","country_code":"TR","type":"education","lineage":["https://openalex.org/I4210128276"]}],"countries":["TR"],"is_corresponding":true,"raw_author_name":"Allah Rakha","raw_affiliation_strings":["University of Health Sciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Health Sciences","institution_ids":["https://openalex.org/I4210128276"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5077666664","https://openalex.org/A5082551108"],"corresponding_institution_ids":["https://openalex.org/I4210128276"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.84652666,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"1","last_page":"14"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10800","display_name":"Forensic Toxicology and Drug Analysis","score":1.0,"subfield":{"id":"https://openalex.org/subfields/3005","display_name":"Toxicology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10800","display_name":"Forensic Toxicology and Drug Analysis","score":1.0,"subfield":{"id":"https://openalex.org/subfields/3005","display_name":"Toxicology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11460","display_name":"Analytical Methods in Pharmaceuticals","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10180","display_name":"Analytical chemistry methods development","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/drug-facilitated-sexual-assault","display_name":"drug-facilitated sexual assault","score":0.9440000057220459},{"id":"https://openalex.org/keywords/designer-benzodiazepines","display_name":"designer benzodiazepines","score":0.8830000162124634},{"id":"https://openalex.org/keywords/forensic-toxicology","display_name":"forensic toxicology","score":0.9670000076293945},{"id":"https://openalex.org/keywords/solid-phase-extraction","display_name":"solid-phase extraction","score":0.8199999928474426},{"id":"https://openalex.org/keywords/uhplc-ms-ms-method-validation","display_name":"UHPLC-MS/MS method validation","score":0.8270000219345093},{"id":"https://openalex.org/keywords/drug-quantification-in-biological-samples","display_name":"drug quantification in biological samples","score":0.4410000145435333}],"concepts":[{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.640999972820282},{"id":"https://openalex.org/C158342456","wikidata":"https://www.wikidata.org/wiki/Q587862","display_name":"Forensic toxicology","level":2,"score":0.5849000215530396},{"id":"https://openalex.org/C40684141","wikidata":"https://www.wikidata.org/wiki/Q902061","display_name":"Electrospray ionization","level":3,"score":0.5105999708175659},{"id":"https://openalex.org/C2994210853","wikidata":"https://www.wikidata.org/wiki/Q673281","display_name":"Sexual assault","level":4,"score":0.507099986076355},{"id":"https://openalex.org/C106487976","wikidata":"https://www.wikidata.org/wiki/Q685816","display_name":"Matrix (chemical analysis)","level":2,"score":0.48080000281333923},{"id":"https://openalex.org/C119128265","wikidata":"https://www.wikidata.org/wiki/Q900165","display_name":"Detection limit","level":2,"score":0.4632999897003174},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.4083999991416931},{"id":"https://openalex.org/C194451485","wikidata":"https://www.wikidata.org/wiki/Q1327575","display_name":"Electrospray","level":3,"score":0.3824000060558319},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.37119999527931213},{"id":"https://openalex.org/C77170095","wikidata":"https://www.wikidata.org/wiki/Q1753188","display_name":"Linearity","level":2,"score":0.35269999504089355},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3508000075817108},{"id":"https://openalex.org/C175467004","wikidata":"https://www.wikidata.org/wiki/Q17088083","display_name":"Ion suppression in liquid chromatography\u2013mass spectrometry","level":4,"score":0.3334999978542328},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.33169999718666077},{"id":"https://openalex.org/C202799725","wikidata":"https://www.wikidata.org/wiki/Q272035","display_name":"Accuracy and precision","level":2,"score":0.30869999527931213},{"id":"https://openalex.org/C2778093475","wikidata":"https://www.wikidata.org/wiki/Q9715","display_name":"Semen","level":2,"score":0.3043999969959259},{"id":"https://openalex.org/C3020728648","wikidata":"https://www.wikidata.org/wiki/Q1058795","display_name":"Biological fluids","level":2,"score":0.2865000069141388},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.28619998693466187},{"id":"https://openalex.org/C3018395757","wikidata":"https://www.wikidata.org/wiki/Q1379672","display_name":"Evaluation methods","level":2,"score":0.2752000093460083},{"id":"https://openalex.org/C2988629283","wikidata":"https://www.wikidata.org/wiki/Q44337","display_name":"Complex matrix","level":2,"score":0.2662000060081482},{"id":"https://openalex.org/C171906077","wikidata":"https://www.wikidata.org/wiki/Q1360677","display_name":"Crime scene","level":2,"score":0.2619999945163727},{"id":"https://openalex.org/C64489805","wikidata":"https://www.wikidata.org/wiki/Q873864","display_name":"Liquid chromatography\u2013mass spectrometry","level":3,"score":0.25609999895095825},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.25589999556541443},{"id":"https://openalex.org/C170814935","wikidata":"https://www.wikidata.org/wiki/Q4751159","display_name":"Analytical technique","level":2,"score":0.2502000033855438},{"id":"https://openalex.org/C81426234","wikidata":"https://www.wikidata.org/wiki/Q1408763","display_name":"Solid phase extraction","level":3,"score":0.25}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1080/17576180.2026.2758060","doi":"https://doi.org/10.1080/17576180.2026.2758060","is_oa":false,"landing_page_url":"https://doi.org/10.1080/17576180.2026.2758060","pdf_url":null,"source":{"id":"https://openalex.org/S159955403","display_name":"Bioanalysis","issn_l":"1757-6180","issn":["1757-6180","1757-6199"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioanalysis","raw_type":"journal-article"},{"id":"pmid:42978305","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42978305","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioanalysis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":65,"referenced_works":["https://openalex.org/W1973544026","https://openalex.org/W1991430603","https://openalex.org/W2050226999","https://openalex.org/W2054707534","https://openalex.org/W2110997670","https://openalex.org/W2144645142","https://openalex.org/W2163402540","https://openalex.org/W2264719596","https://openalex.org/W2328102813","https://openalex.org/W2522967918","https://openalex.org/W2780352969","https://openalex.org/W2913050467","https://openalex.org/W2917135447","https://openalex.org/W2996710301","https://openalex.org/W3034558274","https://openalex.org/W3039439932","https://openalex.org/W3067096154","https://openalex.org/W3092136049","https://openalex.org/W3116414261","https://openalex.org/W3127588062","https://openalex.org/W3129135760","https://openalex.org/W3132186061","https://openalex.org/W3136567069","https://openalex.org/W3187546042","https://openalex.org/W3195443672","https://openalex.org/W3196572710","https://openalex.org/W4206475197","https://openalex.org/W4220903303","https://openalex.org/W4225666514","https://openalex.org/W4233195414","https://openalex.org/W4254185355","https://openalex.org/W4281689614","https://openalex.org/W4283524070","https://openalex.org/W4292687860","https://openalex.org/W4316804829","https://openalex.org/W4316926657","https://openalex.org/W4318373960","https://openalex.org/W4323263382","https://openalex.org/W4372295434","https://openalex.org/W4388427438","https://openalex.org/W4391684818","https://openalex.org/W4392900785","https://openalex.org/W4396858508","https://openalex.org/W4398201150","https://openalex.org/W4399139363","https://openalex.org/W4401794371","https://openalex.org/W4407779389","https://openalex.org/W4408620577","https://openalex.org/W4409242023","https://openalex.org/W4409678335","https://openalex.org/W4410119486","https://openalex.org/W4410194342","https://openalex.org/W4411710485","https://openalex.org/W4411729249","https://openalex.org/W4412942786","https://openalex.org/W4412957992","https://openalex.org/W4413112037","https://openalex.org/W4413440500","https://openalex.org/W4414520234","https://openalex.org/W4416096549","https://openalex.org/W4416621793","https://openalex.org/W4417158020","https://openalex.org/W7127625457","https://openalex.org/W7131814893","https://openalex.org/W7151408227"],"related_works":["https://openalex.org/W4281988781","https://openalex.org/W2924215433","https://openalex.org/W179182294","https://openalex.org/W1972311592","https://openalex.org/W4413440500","https://openalex.org/W3067096154","https://openalex.org/W7120801428","https://openalex.org/W2082403514","https://openalex.org/W2049143747","https://openalex.org/W2016001358"],"abstract_inverted_index":{"Aims":[0],"Flualprazolam":[1],"is":[2],"a":[3,59],"novel":[4],"designer":[5],"benzodiazepine":[6],"frequently":[7],"reported":[8],"in":[9,19,40,126],"drug-facilitated":[10],"sexual":[11],"assaults,":[12],"yet":[13],"validated":[14,32,116],"methods":[15,45],"for":[16,33,130],"its":[17],"quantification":[18,34,89,120],"human":[20,41,127],"seminal":[21,42,128],"fluid":[22,129],"remain":[23],"limited.":[24],"A":[25],"sensitive":[26,119],"method":[27,53,72,108,117],"was":[28,54,109],"therefore":[29],"developed":[30,55],"and":[31,37,44,66,85,93,98,123],"of":[35,82,88,121],"flualprazolam":[36,39,122,125],"\u03b1-hydroxy":[38,124],"fluid.Materials":[43],"An":[46],"ultra-high-performance":[47],"liquid":[48],"chromatography\u2013tandem":[49],"mass":[50],"spectrometry":[51],"(UHPLC\u2013MS/MS)":[52],"using":[56],"solid-phase":[57],"extraction,":[58],"phenyl-hexyl":[60],"reversed-phase":[61],"column,":[62],"positive":[63],"electrospray":[64],"ionization":[65],"multiple":[67],"reaction":[68],"monitoring.":[69],"Results:":[70],"The":[71,107,115],"showed":[73],"linearity":[74],"over":[75],"0.5\u2013200":[76],"ng/mL":[77],"(R2":[78],"\u2265":[79],"0.99).":[80],"Limits":[81],"detection":[83],"(LOD)":[84],"lower":[86],"limits":[87],"(LLOQ)":[90],"were":[91,100],"0.250":[92],"0.500":[94],"ng/mL,":[95],"respectively.":[96],"Accuracy":[97],"precision":[99],"within":[101],"\u00b120%,":[102],"with":[103],"extraction":[104],"recovery":[105],">81%.":[106],"applied":[110],"to":[111],"100":[112],"seminal-fluid":[113],"samples.Conclusions":[114],"enables":[118],"forensic":[131],"toxicological":[132],"applications.":[133]},"counts_by_year":[],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2026-11-05T00:00:00"}