{"id":"https://openalex.org/W7225167643","doi":"https://doi.org/10.64898/2026.11.03.763607","title":"Influence of antibody-antigen interaction on fluorescence of Rhodamine 6G conjugated to the antibodies measured in human serum","display_name":"Influence of antibody-antigen interaction on fluorescence of Rhodamine 6G conjugated to the antibodies measured in human serum","publication_year":2026,"publication_date":"2026-11-03","ids":{"openalex":"https://openalex.org/W7225167643","doi":"https://doi.org/10.64898/2026.11.03.763607"},"language":"en","primary_location":{"id":"doi:10.64898/2026.11.03.763607","doi":"https://doi.org/10.64898/2026.11.03.763607","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.03.763607","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/03/2026.11.03.763607.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/03/2026.11.03.763607.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5141161538","display_name":"Dinesh Thiyagaraj","orcid":"https://orcid.org/0000-0002-2001-0009","observed_orcids":["https://orcid.org/0000-0002-2001-0009"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Dinesh Thiyagaraj","raw_affiliation_strings":["ICHORTec GmbH;"],"raw_orcid":"https://orcid.org/0000-0002-2001-0009","affiliations":[{"raw_affiliation_string":"ICHORTec GmbH;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110503677","display_name":"Alessandro Del Re","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Alessandro Del Re","raw_affiliation_strings":["ICHORTec GmbH;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICHORTec GmbH;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5141165369","display_name":"Quan Duc Pham","orcid":"https://orcid.org/0000-0002-5000-1138","observed_orcids":["https://orcid.org/0000-0002-5000-1138"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Quan Duc Pham","raw_affiliation_strings":["ICHORTec GmbH;"],"raw_orcid":"https://orcid.org/0000-0002-5000-1138","affiliations":[{"raw_affiliation_string":"ICHORTec GmbH;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5141151724","display_name":"Irene Gomez Garrote","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Irene G\u00f3mez Garrote","raw_affiliation_strings":["ICHORTec GmbH;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICHORTec GmbH;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031402090","display_name":"Ahmad Saudi","orcid":"https://orcid.org/0000-0002-7712-6889","observed_orcids":["https://orcid.org/0000-0002-7712-6889","https://orcid.org/0000-0003-2927-3961"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ahmad Saudi","raw_affiliation_strings":["ICHORTec GmbH;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ICHORTec GmbH;","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5106554814","display_name":"Oleh Fedorych","orcid":"https://orcid.org/0009-0004-8429-7979","observed_orcids":["https://orcid.org/0009-0004-8429-7979"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Oleh Fedorych","raw_affiliation_strings":["ICHORtec GmbH"],"raw_orcid":"https://orcid.org/0009-0004-8429-7979","affiliations":[{"raw_affiliation_string":"ICHORtec GmbH","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5141161538"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.36959999799728394,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.36959999799728394,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11129","display_name":"Photochemistry and Electron Transfer Studies","score":0.2653999924659729,"subfield":{"id":"https://openalex.org/subfields/1606","display_name":"Physical and Theoretical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11908","display_name":"Protein Interaction Studies and Fluorescence Analysis","score":0.1348000019788742,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/antibody-antigen-interaction","display_name":"antigen-antibody reaction","score":0.8510000109672546},{"id":"https://openalex.org/keywords/antibody-antibody-interaction","display_name":"antibody-antibody interaction","score":0.875},{"id":"https://openalex.org/keywords/rhodamine-6g","display_name":"rhodamine 6G","score":0.8859999775886536},{"id":"https://openalex.org/keywords/fluorescent-dye-conjugation","display_name":"fluorescent dye conjugation","score":0.718999981880188},{"id":"https://openalex.org/keywords/trastuzumab","display_name":"trastuzumab","score":0.9670000076293945},{"id":"https://openalex.org/keywords/pertuzumab","display_name":"pertuzumab","score":1.0},{"id":"https://openalex.org/keywords/her2","display_name":"HER2","score":0.8119999766349792},{"id":"https://openalex.org/keywords/human-serum","display_name":"human serum","score":0.8299999833106995},{"id":"https://openalex.org/keywords/fluorescent-biosensor","display_name":"fluorescent biosensor","score":0.3109999895095825}],"concepts":[{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.7595999836921692},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7444999814033508},{"id":"https://openalex.org/C112613896","wikidata":"https://www.wikidata.org/wiki/Q898370","display_name":"Conjugated system","level":3,"score":0.5722000002861023},{"id":"https://openalex.org/C96141758","wikidata":"https://www.wikidata.org/wiki/Q216817","display_name":"Emission spectrum","level":3,"score":0.5615000128746033},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5288000106811523},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.48410001397132874},{"id":"https://openalex.org/C2779916640","wikidata":"https://www.wikidata.org/wiki/Q420410","display_name":"Rhodamine","level":3,"score":0.4754999876022339},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.4334999918937683},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.3621000051498413},{"id":"https://openalex.org/C4839761","wikidata":"https://www.wikidata.org/wiki/Q212111","display_name":"Spectral line","level":2,"score":0.3336000144481659},{"id":"https://openalex.org/C2778589691","wikidata":"https://www.wikidata.org/wiki/Q3429577","display_name":"Rhodamine 123","level":4,"score":0.3330000042915344},{"id":"https://openalex.org/C75473681","wikidata":"https://www.wikidata.org/wiki/Q188651","display_name":"Photochemistry","level":1,"score":0.32710000872612},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.31209999322891235},{"id":"https://openalex.org/C2777829344","wikidata":"https://www.wikidata.org/wiki/Q3429576","display_name":"Rhodamine 6G","level":3,"score":0.3050000071525574},{"id":"https://openalex.org/C158711907","wikidata":"https://www.wikidata.org/wiki/Q1768467","display_name":"Fluorescence spectroscopy","level":3,"score":0.2994999885559082},{"id":"https://openalex.org/C106447425","wikidata":"https://www.wikidata.org/wiki/Q11379","display_name":"Energy transfer","level":2,"score":0.2971000075340271},{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.2888000011444092},{"id":"https://openalex.org/C2985906921","wikidata":"https://www.wikidata.org/wiki/Q190524","display_name":"Spectral properties","level":2,"score":0.2624000012874603},{"id":"https://openalex.org/C2780898057","wikidata":"https://www.wikidata.org/wiki/Q79448","display_name":"Immunoglobulin G","level":3,"score":0.25360000133514404}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.11.03.763607","doi":"https://doi.org/10.64898/2026.11.03.763607","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.03.763607","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/03/2026.11.03.763607.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.11.03.763607","doi":"https://doi.org/10.64898/2026.11.03.763607","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.11.03.763607","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/03/2026.11.03.763607.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.4043000042438507,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225167643.pdf","grobid_xml":"https://content.openalex.org/works/W7225167643.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7100405728","https://openalex.org/W2055718411","https://openalex.org/W2544412564","https://openalex.org/W2010584191","https://openalex.org/W2009868255","https://openalex.org/W2119928873","https://openalex.org/W7118190496","https://openalex.org/W2349220660","https://openalex.org/W2038598445","https://openalex.org/W19634196"],"abstract_inverted_index":{"Fluorescent":[0],"dyes":[1],"conjugated":[2],"to":[3],"monoclonal":[4],"antibodies":[5,178],"specifically":[6],"trastuzumab":[7],"(TRAS)":[8],"and":[9,26,51,71,80,105,108,113,123,179,196],"pertuzumab":[10],"(PERT)":[11],"serve":[12],"as":[13,88,200],"sensitive":[14],"reporters":[15],"of":[16,40,46,54,59,83,91,132,167],"changes":[17,45,152],"in":[18,44,68,103,106,146,208],"the":[19,32,37,41,47,55,77,120,130,133,140,149,168],"local":[20,34],"energetic":[21,38],"environment":[22,35],"arising":[23],"from":[24],"antibody-antibody":[25],"antibody-antigen":[27,114,159],"interactions.":[28],"Upon":[29],"covalent":[30],"conjugation,":[31],"antibody":[33,64,206],"perturbates":[36],"state":[39],"dye,":[42],"resulting":[43],"fluorescence":[48,193],"emission":[49,52,78,81,121,124,194,197],"energy":[50,79,122,195],"width":[53,82,198],"dye.":[56],"The":[57],"dependence":[58],"these":[60],"spectral":[61,151],"parameters":[62],"on":[63,129],"concentration":[65,102,165],"was":[66],"measured":[67],"HBS-8,":[69],"diluted":[70,107],"undiluted":[72,109],"human":[73,93,110],"serum.":[74,111],"In":[75],"addition,":[76],"dye-conjugated":[84,177],"TRAS":[85,145],"were":[86],"investigated":[87],"a":[89,201],"function":[90],"recombinant":[92],"epidermal":[94],"growth":[95],"factor":[96],"receptor":[97],"2":[98],"(HER2)":[99],"extracellular":[100],"domain":[101],"HBS-8":[104],"Antibody-antibody":[112],"interactions":[115,175,207],"produce":[116],"remarkable":[117],"contributions":[118,127],"into":[119],"width.":[125],"These":[126,190],"depend":[128],"composition":[131],"surrounding":[134],"medium.":[135],"Our":[136],"study":[137],"showed":[138],"that":[139,158,174],"samples":[141],"containing":[142],"100":[143],"nM":[144],"serum":[147],"exhibited":[148],"largest":[150],"upon":[153],"interaction":[154,160],"with":[155],"HER2":[156],"suggesting":[157],"are":[161],"not":[162],"linear":[163],"across":[164],"range":[166],"antibodies.":[169],"Moreover,":[170],"our":[171],"results":[172],"demonstrate":[173],"between":[176],"their":[180],"binding":[181],"partners":[182],"can":[183],"be":[184],"detected":[185],"under":[186],"physiologically":[187],"relevant":[188],"conditions.":[189],"findings":[191],"establish":[192],"analysis":[199],"potential":[202],"approach":[203],"for":[204],"probing":[205],"complex":[209],"biological":[210],"matrices.":[211]},"counts_by_year":[],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2026-11-04T00:00:00"}
