{"id":"https://openalex.org/W7225148106","doi":"https://doi.org/10.1136/jitc-2026-sitc2026.0924","title":"924 Harnessing VLPs & banodiscs to unlock antibody discovery for challenging membrane proteins","display_name":"924 Harnessing VLPs & banodiscs to unlock antibody discovery for challenging membrane proteins","publication_year":2026,"publication_date":"2026-11-01","ids":{"openalex":"https://openalex.org/W7225148106","doi":"https://doi.org/10.1136/jitc-2026-sitc2026.0924"},"language":"en","primary_location":{"id":"doi:10.1136/jitc-2026-sitc2026.0924","doi":"https://doi.org/10.1136/jitc-2026-sitc2026.0924","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2026-sitc2026.0924","pdf_url":"https://jitc.bmj.com/content/14/Suppl_2/A1048.full.pdf","source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular Abstracts","raw_type":"proceedings-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://jitc.bmj.com/content/14/Suppl_2/A1048.full.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5148765515","display_name":"Maggie Gao","orcid":"https://orcid.org/0009-0005-0407-9339","observed_orcids":["https://orcid.org/0009-0005-0407-9339"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Maggie Gao","raw_affiliation_strings":["KACTUS, Waltham, MA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"KACTUS, Waltham, MA, USA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5148765515"],"corresponding_institution_ids":[],"apc_list":{"value":2490,"currency":"USD","value_usd":2490},"apc_paid":{"value":2490,"currency":"USD","value_usd":2490},"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"A1048","last_page":"A1048"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.5464000105857849,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.5464000105857849,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.3959999978542328,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0066999997943639755,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/virus-like-particles","display_name":"virus-like particles","score":0.9819999933242798},{"id":"https://openalex.org/keywords/antibody-discovery","display_name":"antibody discovery","score":0.8429999947547913},{"id":"https://openalex.org/keywords/cxcr4","display_name":"CXCR4","score":0.7979999780654907},{"id":"https://openalex.org/keywords/t-cell-receptor-cd3-complex","display_name":"T cell receptor/CD3 complex","score":0.8479999899864197},{"id":"https://openalex.org/keywords/adenosine-a2a-receptor","display_name":"adenosine A2A receptor","score":0.8840000033378601},{"id":"https://openalex.org/keywords/gprc5d","display_name":"GPRC5D","score":0.8659999966621399},{"id":"https://openalex.org/keywords/steap1","display_name":"STEAP1","score":0.9940000176429749},{"id":"https://openalex.org/keywords/anti-cd3-antibody","display_name":"anti-CD3 antibody","score":0.828000009059906},{"id":"https://openalex.org/keywords/g-protein-coupled-receptors","display_name":"G protein-coupled receptors","score":0.800000011920929}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5389000177383423},{"id":"https://openalex.org/C74187038","wikidata":"https://www.wikidata.org/wiki/Q1418791","display_name":"Drug discovery","level":2,"score":0.5051000118255615},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5022000074386597},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.4945000112056732},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.4697999954223633},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.36070001125335693},{"id":"https://openalex.org/C57581600","wikidata":"https://www.wikidata.org/wiki/Q2449730","display_name":"Transport protein","level":2,"score":0.3384999930858612},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.32739999890327454},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.296999990940094},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.2782000005245209},{"id":"https://openalex.org/C191120209","wikidata":"https://www.wikidata.org/wiki/Q908902","display_name":"Structural biology","level":2,"score":0.27149999141693115},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.2685999870300293},{"id":"https://openalex.org/C2908856526","wikidata":"https://www.wikidata.org/wiki/Q16252627","display_name":"Antibody therapy","level":4,"score":0.26840001344680786},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.26260000467300415},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.2612999975681305},{"id":"https://openalex.org/C11804247","wikidata":"https://www.wikidata.org/wiki/Q896177","display_name":"Protein\u2013protein interaction","level":2,"score":0.25780001282691956},{"id":"https://openalex.org/C51639874","wikidata":"https://www.wikidata.org/wiki/Q167149","display_name":"Plasma protein binding","level":2,"score":0.25760000944137573},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.25060001015663147}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1136/jitc-2026-sitc2026.0924","doi":"https://doi.org/10.1136/jitc-2026-sitc2026.0924","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2026-sitc2026.0924","pdf_url":"https://jitc.bmj.com/content/14/Suppl_2/A1048.full.pdf","source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular Abstracts","raw_type":"proceedings-article"}],"best_oa_location":{"id":"doi:10.1136/jitc-2026-sitc2026.0924","doi":"https://doi.org/10.1136/jitc-2026-sitc2026.0924","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2026-sitc2026.0924","pdf_url":"https://jitc.bmj.com/content/14/Suppl_2/A1048.full.pdf","source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular Abstracts","raw_type":"proceedings-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8048999905586243,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225148106.pdf"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7225157549","https://openalex.org/W7148882514","https://openalex.org/W4400496617","https://openalex.org/W7110617840","https://openalex.org/W1646595952","https://openalex.org/W2945836308","https://openalex.org/W2645388367","https://openalex.org/W4318015885","https://openalex.org/W7220750968","https://openalex.org/W2015540010"],"abstract_inverted_index":{"Background":[0],"Multi-pass":[1],"transmembrane":[2],"proteins,":[3],"including":[4,166,218],"G":[5],"protein-coupled":[6],"receptors,":[7],"ion":[8],"channels,":[9],"transporters,":[10],"and":[11,39,53,73,108,122,132,141,147,158,173,180,189,208,234,250,265,290,307],"immune":[12,287],"receptor":[13,151,198,288],"complexes,":[14,175],"represent":[15],"a":[16,27,219,228],"large":[17],"class":[18],"of":[19,252,274,302],"validated":[20],"therapeutic":[21],"targets.":[22],"However,":[23],"their":[24,43,256],"dependence":[25],"on":[26,81],"native":[28,178],"membrane":[29,63,77,163,276,296],"environment":[30],"creates":[31],"significant":[32],"challenges":[33],"for":[34,69,129,143,154,312],"recombinant":[35],"expression,":[36],"structural":[37],"stability,":[38],"functional":[40,216,300],"characterization,":[41,289],"limiting":[42],"utility":[44],"in":[45,65,84,240,258,279],"antibody":[46,70,134,155,191,261,285],"discovery.":[47,135],"We":[48],"developed":[49],"virus-like":[50],"particle":[51],"(VLP)":[52],"styrene":[54],"maleic":[55],"acid":[56],"(SMA)-based":[57],"nanodisc":[58,267],"platforms":[59,268],"to":[60,227],"display":[61],"full-length":[62],"proteins":[64,78],"native-like":[66,280],"lipid":[67],"bilayers":[68],"generation,":[71],"screening,":[72],"bioanalytical":[74],"applications.Methods":[75],"Full-length":[76],"were":[79],"displayed":[80,161],"VLPs":[82,140,157,185,214,233,306],"generated":[83,215],"mammalian":[85],"cells":[86],"or":[87],"incorporated":[88],"into":[89],"SMA-based":[90,266],"nanodiscs":[91,142,159,199,235,308],"using":[92,115],"detergent-free":[93],"extraction":[94],"methods.":[95],"Biophysical":[96],"characterization":[97],"included":[98],"transmission":[99],"electron":[100],"microscopy,":[101],"dynamic":[102],"light":[103],"scattering,":[104],"high-performance":[105],"liquid":[106],"chromatography,":[107],"purity":[109,188],"analysis.":[110],"Antigen":[111],"functionality":[112],"was":[113,127],"evaluated":[114],"enzyme-linked":[116,194],"immunosorbent":[117,195],"assay,":[118],"surface":[119,205],"plasmon":[120,206],"resonance,":[121],"biolayer":[123,209],"interferometry.":[124,210],"VLP-displayed":[125],"CXCR4":[126,213],"used":[128],"mouse":[130],"immunization":[131],"monoclonal":[133],"Additional":[136],"studies":[137],"assessed":[138],"biotinylated":[139],"phage":[144],"panning":[145],"applications":[146],"nanodisc-displayed":[148],"T":[149],"cell":[150],"(TCR)-CD3":[152],"complexes":[153,246],"screening.Results":[156],"successfully":[160],"multiple":[162],"protein":[164,277],"classes,":[165],"STEAP1,":[167],"CXCR4,":[168],"adenosine":[169],"A2A":[170,197],"receptor,":[171],"GPRC5D,":[172],"TCR-CD3":[174,245],"while":[176],"maintaining":[177],"conformations":[179],"robust":[181],"binding":[182,192,238,249],"activity.":[183],"STEAP1":[184],"demonstrated":[186,223],"high":[187],"specific":[190],"by":[193,204],"assay.":[196],"exhibited":[200],"nanomolar":[201],"affinity":[202],"interactions":[203],"resonance":[207],"Immunization":[211],"with":[212],"antibodies,":[217,254],"lead":[220],"clone":[221],"that":[222],"ligand-blocking":[224],"activity":[225],"comparable":[226],"commercial":[229],"anti-CXCR4":[230],"antibody.":[231],"Biotinylated":[232],"produced":[236],"strong":[237],"signals":[239],"phage-panning":[241],"compatible":[242],"assays.":[243],"Nanodisc-displayed":[244],"enabled":[247],"high-affinity":[248],"screening":[251],"anti-CD3":[253],"supporting":[255],"use":[257],"biologically":[259,271],"relevant":[260,295],"discovery":[262],"workflows.Conclusions":[263],"VLP":[264],"provide":[269],"robust,":[270],"active":[272],"presentation":[273],"challenging":[275],"targets":[278],"conformations.":[281],"These":[282],"technologies":[283],"support":[284],"discovery,":[286],"assay":[291],"development":[292],"against":[293],"therapeutically":[294],"proteins.":[297],"By":[298],"enabling":[299],"interrogation":[301],"previously":[303],"difficult-to-access":[304],"targets,":[305],"offer":[309],"versatile":[310],"tools":[311],"advancing":[313],"next-generation":[314],"immunotherapies.":[315]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2026-11-04T00:00:00"}