{"id":"https://openalex.org/W7225110256","doi":"https://doi.org/10.1186/s13287-026-05385-8","title":"ALKBH5-mediated m\u2076A demethylation stabilizes BMP-2 mRNA via YTHDF2 to drive pathological osteogenesis in ankylosing spondylitis-derived mesenchymal stem cells","display_name":"ALKBH5-mediated m\u2076A demethylation stabilizes BMP-2 mRNA via YTHDF2 to drive pathological osteogenesis in ankylosing spondylitis-derived mesenchymal stem cells","publication_year":2026,"publication_date":"2026-11-02","ids":{"openalex":"https://openalex.org/W7225110256","doi":"https://doi.org/10.1186/s13287-026-05385-8"},"language":"en","primary_location":{"id":"doi:10.1186/s13287-026-05385-8","doi":"https://doi.org/10.1186/s13287-026-05385-8","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13287-026-05385-8","pdf_url":null,"source":{"id":"https://openalex.org/S2061155","display_name":"Stem Cell Research & Therapy","issn_l":"1757-6512","issn":["1757-6512"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stem Cell Research & Therapy","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1186/s13287-026-05385-8","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5134108832","display_name":"Jiu Zhao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]},{"id":"https://openalex.org/I4387154481","display_name":"Eighth Affiliated Hospital of Sun Yat-sen University","ror":"https://ror.org/00xjwyj62","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4387154481"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jiu Zhao","raw_affiliation_strings":["Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China","institution_ids":["https://openalex.org/I157773358","https://openalex.org/I4387154481"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014073691","display_name":"Sijia Meng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]},{"id":"https://openalex.org/I4387154481","display_name":"Eighth Affiliated Hospital of Sun Yat-sen University","ror":"https://ror.org/00xjwyj62","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4387154481"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shuhao Meng","raw_affiliation_strings":["Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China","institution_ids":["https://openalex.org/I157773358","https://openalex.org/I4387154481"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160351762","display_name":"Xinxing Ning","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]},{"id":"https://openalex.org/I4387154481","display_name":"Eighth Affiliated Hospital of Sun Yat-sen University","ror":"https://ror.org/00xjwyj62","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4387154481"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xinxing Ning","raw_affiliation_strings":["Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China","institution_ids":["https://openalex.org/I157773358","https://openalex.org/I4387154481"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113170591","display_name":"Yipeng Zeng","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]},{"id":"https://openalex.org/I4387154481","display_name":"Eighth Affiliated Hospital of Sun Yat-sen University","ror":"https://ror.org/00xjwyj62","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4387154481"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yipeng Zeng","raw_affiliation_strings":["Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China","institution_ids":["https://openalex.org/I157773358","https://openalex.org/I4387154481"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160347653","display_name":"Qibo Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]},{"id":"https://openalex.org/I4387154481","display_name":"Eighth Affiliated Hospital of Sun Yat-sen University","ror":"https://ror.org/00xjwyj62","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4387154481"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qibo Li","raw_affiliation_strings":["Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China","institution_ids":["https://openalex.org/I157773358","https://openalex.org/I4387154481"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006348763","display_name":"Weihao Zhang","orcid":"https://orcid.org/0009-0004-7378-4333","observed_orcids":["https://orcid.org/0009-0004-7378-4333"]},"institutions":[{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]},{"id":"https://openalex.org/I4387154481","display_name":"Eighth Affiliated Hospital of Sun Yat-sen University","ror":"https://ror.org/00xjwyj62","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4387154481"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Weihao Zhang","raw_affiliation_strings":["Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China","institution_ids":["https://openalex.org/I157773358","https://openalex.org/I4387154481"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103191608","display_name":"Zhongyu Xie","orcid":"https://orcid.org/0000-0003-2665-6115","observed_orcids":["https://orcid.org/0000-0003-2665-6115"]},"institutions":[{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]},{"id":"https://openalex.org/I4387154481","display_name":"Eighth Affiliated Hospital of Sun Yat-sen University","ror":"https://ror.org/00xjwyj62","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4387154481"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zhongyu Xie","raw_affiliation_strings":["Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China"],"raw_orcid":"https://orcid.org/0000-0003-2665-6115","affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China","institution_ids":["https://openalex.org/I157773358","https://openalex.org/I4387154481"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112479127","display_name":"Wenhui Yu","orcid":"https://orcid.org/0000-0002-0684-5237","observed_orcids":["https://orcid.org/0000-0002-0684-5237"]},"institutions":[{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]},{"id":"https://openalex.org/I4387154481","display_name":"Eighth Affiliated Hospital of Sun Yat-sen University","ror":"https://ror.org/00xjwyj62","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4387154481"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Wenhui Yu","raw_affiliation_strings":["Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China"],"raw_orcid":"https://orcid.org/0000-0002-0684-5237","affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China","institution_ids":["https://openalex.org/I157773358","https://openalex.org/I4387154481"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160349996","display_name":"Shan Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]},{"id":"https://openalex.org/I4387154481","display_name":"Eighth Affiliated Hospital of Sun Yat-sen University","ror":"https://ror.org/00xjwyj62","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4387154481"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Shan Wang","raw_affiliation_strings":["Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China","institution_ids":["https://openalex.org/I157773358","https://openalex.org/I4387154481"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5123403885","display_name":"Guan Zheng","orcid":"https://orcid.org/0000-0001-8634-1030","observed_orcids":["https://orcid.org/0000-0001-8634-1030"]},"institutions":[{"id":"https://openalex.org/I157773358","display_name":"Sun Yat-sen University","ror":"https://ror.org/0064kty71","country_code":"CN","type":"education","lineage":["https://openalex.org/I157773358"]},{"id":"https://openalex.org/I4387154481","display_name":"Eighth Affiliated Hospital of Sun Yat-sen University","ror":"https://ror.org/00xjwyj62","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4387154481"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Guan Zheng","raw_affiliation_strings":["Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China"],"raw_orcid":"https://orcid.org/0000-0001-8634-1030","affiliations":[{"raw_affiliation_string":"Department of Orthopedics, The Eighth Affiliated Hospital, Sun Yat-sen University, 3025# Shennan Road, Shenzhen, 518000, PR China","institution_ids":["https://openalex.org/I157773358","https://openalex.org/I4387154481"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5112479127","https://openalex.org/A5123403885","https://openalex.org/A5160349996"],"corresponding_institution_ids":["https://openalex.org/I157773358","https://openalex.org/I4387154481"],"apc_list":{"value":3190,"currency":"USD","value_usd":3190},"apc_paid":{"value":3190,"currency":"USD","value_usd":3190},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.7533849,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11482","display_name":"RNA modifications and cancer","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ankylosing-spondylitis","display_name":"ankylosing spondylitis","score":0.9229999780654907},{"id":"https://openalex.org/keywords/alkbh5","display_name":"ALKBH5","score":0.8550000190734863},{"id":"https://openalex.org/keywords/m6a-demethylation","display_name":"m6A demethylation","score":0.8550000190734863},{"id":"https://openalex.org/keywords/ythdf2","display_name":"YTHDF2","score":0.9100000262260437},{"id":"https://openalex.org/keywords/bmp-2","display_name":"BMP-2","score":0.9729999899864197},{"id":"https://openalex.org/keywords/mrna-stability","display_name":"mRNA stability","score":0.7250000238418579},{"id":"https://openalex.org/keywords/mesenchymal-stem-cells","display_name":"mesenchymal stem cells","score":0.9959999918937683},{"id":"https://openalex.org/keywords/nebivolol","display_name":"nebivolol","score":0.9959999918937683},{"id":"https://openalex.org/keywords/lifitegrast","display_name":"lifitegrast","score":0.9959999918937683},{"id":"https://openalex.org/keywords/m6a-methylation","display_name":"m6A RNA methylation","score":0.5210000276565552},{"id":"https://openalex.org/keywords/bone-formation","display_name":"bone formation","score":0.34299999475479126}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6510000228881836},{"id":"https://openalex.org/C198826908","wikidata":"https://www.wikidata.org/wiki/Q1922379","display_name":"Mesenchymal stem cell","level":2,"score":0.6456999778747559},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5651000142097473},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.5094000101089478},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.5041000247001648},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.41760000586509705},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4027999937534332},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.38109999895095825},{"id":"https://openalex.org/C85265743","wikidata":"https://www.wikidata.org/wiki/Q885458","display_name":"Blot","level":3,"score":0.3353999853134155},{"id":"https://openalex.org/C111829913","wikidata":"https://www.wikidata.org/wiki/Q211083","display_name":"Gene targeting","level":3,"score":0.325300008058548},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2919999957084656},{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.27570000290870667},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.267300009727478},{"id":"https://openalex.org/C148738053","wikidata":"https://www.wikidata.org/wiki/Q210861","display_name":"Cellular differentiation","level":3,"score":0.2612999975681305},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.2612999975681305},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.2612000107765198}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1186/s13287-026-05385-8","doi":"https://doi.org/10.1186/s13287-026-05385-8","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13287-026-05385-8","pdf_url":null,"source":{"id":"https://openalex.org/S2061155","display_name":"Stem Cell Research & Therapy","issn_l":"1757-6512","issn":["1757-6512"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stem Cell Research & Therapy","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1186/s13287-026-05385-8","doi":"https://doi.org/10.1186/s13287-026-05385-8","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13287-026-05385-8","pdf_url":null,"source":{"id":"https://openalex.org/S2061155","display_name":"Stem Cell Research & Therapy","issn_l":"1757-6512","issn":["1757-6512"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stem Cell Research & Therapy","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9789000153541565}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320317337","display_name":"Science and Technology Foundation of Shenzhen City","ror":null},{"id":"https://openalex.org/F4320321921","display_name":"Natural Science Foundation of Guangdong Province","ror":null}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2274426412","https://openalex.org/W4410892467","https://openalex.org/W2917932286","https://openalex.org/W4313345944","https://openalex.org/W2941985102","https://openalex.org/W4404657637","https://openalex.org/W3165806680","https://openalex.org/W3039104601","https://openalex.org/W2780255079","https://openalex.org/W4412438598"],"abstract_inverted_index":{"Pathological":[0],"osteogenesis":[1],"contributes":[2],"significantly":[3,177],"to":[4,96,200],"ankylosing":[5],"spondylitis":[6],"(AS)":[7],"progression,":[8],"with":[9,220],"mesenchymal":[10],"stem":[11],"cells":[12],"(MSCs)":[13],"exhibiting":[14],"increased":[15],"osteogenic":[16,185],"potential.":[17],"While":[18],"BMP-2":[19,62,136,179,201],"overexpression":[20],"has":[21],"been":[22],"observed":[23],"in":[24,105,108,188,191,212,216,231],"AS-MSCs,":[25],"the":[26,53,154,183,207],"precise":[27],"molecular":[28],"mechanisms":[29],"underlying":[30],"its":[31,87],"dysregulation":[32],"remain":[33],"elusive.":[34],"Using":[35],"m\u2076A":[36,46,80,116,121,151],"dot":[37],"blot":[38],"and":[39,44,55,74,107,142,166,181,190],"MeRIP-qPCR,":[40],"we":[41],"quantified":[42],"global":[43],"BMP-2-specific":[45],"modifications":[47],"during":[48,118],"osteogenesis.":[49],"Genetic":[50],"perturbations":[51],"of":[52,206],"ALKBH5":[54,99,133,147,170,197,219],"YTHDF":[56],"genes":[57],"were":[58,65,102],"achieved":[59],"via":[60,204],"siRNA/lentivirus.":[61],"mRNA":[63,137,156,202],"dynamics":[64,117],"investigated":[66],"through":[67],"actinomycin":[68],"D":[69],"chase":[70],"assays,":[71],"subcellular":[72],"fractionation,":[73],"polysome":[75],"profiling.":[76],"Site-directed":[77],"mutagenesis":[78],"confirmed":[79],"functionality,":[81],"whereas":[82],"subcutaneous":[83,110],"implantation":[84,111],"assays":[85],"validated":[86,104],"pathological":[88,184,213,233],"relevance.":[89],"Furthermore,":[90],"structure-based":[91],"virtual":[92],"screening":[93,163],"was":[94],"performed":[95],"identify":[97],"potential":[98,225],"inhibitors,":[100],"which":[101],"subsequently":[103],"vitro":[106,189],"a":[109,224],"model.":[112],"AS-MSCs":[113],"exhibited":[114],"unique":[115],"osteogenesis:":[119],"diminished":[120],"methylation/YTHDF2":[122],"but":[123],"elevated":[124],"ALKBH5/BMP-2":[125],"expression":[126,180],"distinguished":[127],"AS-MSCs.":[128],"Functional":[129,172],"interrogation":[130],"demonstrated":[131],"that":[132,175,196],"ablation":[134],"accelerated":[135],"decay,":[138],"attenuated":[139,153],"protein":[140],"output,":[141],"impaired":[143],"mineralization\u2014phenotypes":[144],"rescued":[145,182],"by":[146],"reconstitution.":[148],"Mechanistically,":[149],"ALKBH5-mediated":[150],"erasure":[152],"YTHDF2-BMP-2":[155],"interaction,":[157],"consequently":[158],"subverting":[159],"transcript":[160],"degradation.":[161],"Virtual":[162],"identified":[164],"lifitegrast":[165],"nebivolol":[167,176,221],"as":[168],"high-affinity":[169],"ligands.":[171],"validation":[173],"revealed":[174],"suppressed":[178],"phenotype":[186],"both":[187],"vivo.":[192],"Our":[193],"data":[194],"suggest":[195],"may":[198,222],"contribute":[199],"stabilization":[203],"regulation":[205],"m\u2076A-YTHDF2":[208],"axis,":[209],"potentially":[210],"participating":[211],"ossification":[214],"processes":[215],"AS.":[217],"Targeting":[218],"represent":[223],"therapeutic":[226],"approach":[227],"for":[228],"further":[229],"investigation":[230],"AS-related":[232],"bone":[234],"formation.":[235]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2026-11-03T00:00:00"}