{"id":"https://openalex.org/W7225100085","doi":"https://doi.org/10.1111/1462-2920.70449","title":"Targeted Mutagenesis Reveals Noncanonical Genetic Requirements for Carbon Monoxide Oxidation","display_name":"Targeted Mutagenesis Reveals Noncanonical Genetic Requirements for Carbon Monoxide Oxidation","publication_year":2026,"publication_date":"2026-11-01","ids":{"openalex":"https://openalex.org/W7225100085","doi":"https://doi.org/10.1111/1462-2920.70449","pmid":"https://pubmed.ncbi.nlm.nih.gov/42971800","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13727707"},"language":"en","primary_location":{"id":"doi:10.1111/1462-2920.70449","doi":"https://doi.org/10.1111/1462-2920.70449","is_oa":true,"landing_page_url":"https://doi.org/10.1111/1462-2920.70449","pdf_url":null,"source":{"id":"https://openalex.org/S6919857","display_name":"Environmental Microbiology","issn_l":"1462-2912","issn":["1462-2912","1462-2920"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1111/1462-2920.70449","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5048552533","display_name":"Robin A. Dawson","orcid":"https://orcid.org/0000-0002-7635-2251","observed_orcids":["https://orcid.org/0000-0002-7635-2251"]},"institutions":[{"id":"https://openalex.org/I1118541","display_name":"University of East Anglia","ror":"https://ror.org/026k5mg93","country_code":"GB","type":"education","lineage":["https://openalex.org/I1118541"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"R. A. Dawson","raw_affiliation_strings":["School of Biological Sciences University of East Anglia  Norwich UK"],"raw_orcid":"https://orcid.org/0000-0002-7635-2251","affiliations":[{"raw_affiliation_string":"School of Biological Sciences University of East Anglia  Norwich UK","institution_ids":["https://openalex.org/I1118541"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084897351","display_name":"Marcela Hern\u00e1ndez","orcid":"https://orcid.org/0000-0002-1041-785X","observed_orcids":["https://orcid.org/0000-0002-1041-785X"]},"institutions":[{"id":"https://openalex.org/I1118541","display_name":"University of East Anglia","ror":"https://ror.org/026k5mg93","country_code":"GB","type":"education","lineage":["https://openalex.org/I1118541"]},{"id":"https://openalex.org/I79619799","display_name":"University of Birmingham","ror":"https://ror.org/03angcq70","country_code":"GB","type":"education","lineage":["https://openalex.org/I79619799"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"M. Hern\u00e1ndez","raw_affiliation_strings":["School of Biological Sciences University of East Anglia  Norwich UK","School of Biosciences University of Birmingham  Birmingham UK"],"raw_orcid":"https://orcid.org/0000-0002-1041-785X","affiliations":[{"raw_affiliation_string":"School of Biological Sciences University of East Anglia  Norwich UK","institution_ids":["https://openalex.org/I1118541"]},{"raw_affiliation_string":"School of Biosciences University of Birmingham  Birmingham UK","institution_ids":["https://openalex.org/I79619799"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5084897351"],"corresponding_institution_ids":["https://openalex.org/I1118541","https://openalex.org/I79619799"],"apc_list":{"value":4640,"currency":"USD","value_usd":4640},"apc_paid":{"value":4640,"currency":"USD","value_usd":4640},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.76444892,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"28","issue":"11","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11626","display_name":"Metalloenzymes and iron-sulfur proteins","score":0.9419000148773193,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11626","display_name":"Metalloenzymes and iron-sulfur proteins","score":0.9419000148773193,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12688","display_name":"Microbial metabolism and enzyme function","score":0.03880000114440918,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11747","display_name":"Metal-Catalyzed Oxygenation Mechanisms","score":0.00930000003427267,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/co-oxidation","display_name":"CO oxidation","score":0.9950000047683716},{"id":"https://openalex.org/keywords/gene-essentiality","display_name":"gene essentiality","score":0.5899999737739563}],"concepts":[{"id":"https://openalex.org/C2780049555","wikidata":"https://www.wikidata.org/wiki/Q1013950","display_name":"Carbon monoxide dehydrogenase","level":4,"score":0.9031999707221985},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.722599983215332},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.7156999707221985},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6991000175476074},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5278000235557556},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.5110999941825867},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.45989999175071716},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.4426000118255615},{"id":"https://openalex.org/C512735826","wikidata":"https://www.wikidata.org/wiki/Q2025","display_name":"Carbon monoxide","level":3,"score":0.3707999885082245},{"id":"https://openalex.org/C69034178","wikidata":"https://www.wikidata.org/wiki/Q4680754","display_name":"Directed mutagenesis","level":4,"score":0.3675999939441681},{"id":"https://openalex.org/C2988890453","wikidata":"https://www.wikidata.org/wiki/Q5508815","display_name":"Functional diversity","level":2,"score":0.3425999879837036},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.3377000093460083},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3230000138282776},{"id":"https://openalex.org/C9418097","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed evolution","level":4,"score":0.31380000710487366},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.299699991941452},{"id":"https://openalex.org/C53479859","wikidata":"https://www.wikidata.org/wiki/Q3064200","display_name":"Trans-acting","level":4,"score":0.2842999994754791},{"id":"https://openalex.org/C2908542902","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed Molecular Evolution","level":5,"score":0.26080000400543213},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.2531999945640564}],"mesh":[{"descriptor_ui":"D000445","descriptor_name":"Aldehyde Oxidoreductases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000445","descriptor_name":"Aldehyde Oxidoreductases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002248","descriptor_name":"Carbon Monoxide","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005810","descriptor_name":"Multigene Family","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009097","descriptor_name":"Multienzyme Complexes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009097","descriptor_name":"Multienzyme Complexes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D042521","descriptor_name":"Burkholderiaceae","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D042521","descriptor_name":"Burkholderiaceae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D042521","descriptor_name":"Burkholderiaceae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1111/1462-2920.70449","doi":"https://doi.org/10.1111/1462-2920.70449","is_oa":true,"landing_page_url":"https://doi.org/10.1111/1462-2920.70449","pdf_url":null,"source":{"id":"https://openalex.org/S6919857","display_name":"Environmental Microbiology","issn_l":"1462-2912","issn":["1462-2912","1462-2920"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Environmental Microbiology","raw_type":"journal-article"},{"id":"pmid:42971800","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42971800","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Environmental microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13727707","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13727707/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Environ Microbiol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1111/1462-2920.70449","doi":"https://doi.org/10.1111/1462-2920.70449","is_oa":true,"landing_page_url":"https://doi.org/10.1111/1462-2920.70449","pdf_url":null,"source":{"id":"https://openalex.org/S6919857","display_name":"Environmental Microbiology","issn_l":"1462-2912","issn":["1462-2912","1462-2920"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Environmental Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320006","display_name":"Royal Society","ror":"https://ror.org/03wnrjx87"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":46,"referenced_works":["https://openalex.org/W1963794591","https://openalex.org/W1967968024","https://openalex.org/W1973458202","https://openalex.org/W1986070864","https://openalex.org/W1991180369","https://openalex.org/W1993374643","https://openalex.org/W1997112841","https://openalex.org/W2010713127","https://openalex.org/W2016537573","https://openalex.org/W2022379622","https://openalex.org/W2029569150","https://openalex.org/W2033639652","https://openalex.org/W2036132662","https://openalex.org/W2036917159","https://openalex.org/W2041836513","https://openalex.org/W2042487179","https://openalex.org/W2043292500","https://openalex.org/W2048134819","https://openalex.org/W2055043387","https://openalex.org/W2068170734","https://openalex.org/W2070828244","https://openalex.org/W2072570060","https://openalex.org/W2083455465","https://openalex.org/W2086358238","https://openalex.org/W2088415326","https://openalex.org/W2104415116","https://openalex.org/W2123957786","https://openalex.org/W2130792768","https://openalex.org/W2131717149","https://openalex.org/W2148879422","https://openalex.org/W2226097824","https://openalex.org/W2408964212","https://openalex.org/W2417207989","https://openalex.org/W2495984948","https://openalex.org/W2801781546","https://openalex.org/W2966149702","https://openalex.org/W3027317375","https://openalex.org/W4223586396","https://openalex.org/W4251751280","https://openalex.org/W4361223535","https://openalex.org/W4384522975","https://openalex.org/W4391111129","https://openalex.org/W4400363963","https://openalex.org/W4406834018","https://openalex.org/W4406931591","https://openalex.org/W4415823570"],"related_works":["https://openalex.org/W4393388201","https://openalex.org/W7164649015","https://openalex.org/W7135014523","https://openalex.org/W2801781546","https://openalex.org/W1963794591","https://openalex.org/W2041836513","https://openalex.org/W2042487179","https://openalex.org/W7170327707","https://openalex.org/W4406931591","https://openalex.org/W4317374086"],"abstract_inverted_index":{"ABSTRACT":[0],"Carbon":[1],"monoxide":[2],"(CO)":[3],"oxidation":[4,152],"is":[5,29],"a":[6,66,76,188],"widespread":[7],"microbial":[8],"energy":[9],"metabolism":[10],"catalysed":[11],"by":[12],"MoCu":[13],"CO":[14,99,151],"dehydrogenase":[15],"(CODH).":[16],"Of":[17],"the":[18,37,45,51,111,140,194],"two":[19],"phylogenetically":[20],"distinct":[21,116],"forms":[22,72],"(form\u2010I":[23],"and":[24,78,94,105,135,167,173,196],"form\u2010II),":[25],"only":[26],"form\u2010I":[27],"CODH":[28,32,55,74,127,146,165],"functionally":[30],"verified.":[31],"activity":[33],"requires":[34],"maturation":[35,56,166],"of":[36,60,73,110,158,198],"[CuSMoO":[38],"2":[39],"]":[40],"active":[41],"site,":[42],"linked":[43],"in":[44,136,153,155,202],"carboxydotroph":[46],"Afipia":[47],"carboxidovorans":[48,121],"OM5":[49],"to":[50,119,150],"genes":[52,114],"coxDEF":[53,113],".":[54],"remains":[57],"untested":[58],"outside":[59],"this":[61],"system.":[62],"Paraburkholderia":[63],"terrae":[64,184],"COX,":[65],"recently":[67],"isolated":[68],"carboxydovore,":[69],"encodes":[70],"both":[71],"with":[75],"reduced":[77],"reorganised":[79],"cox":[80],"gene":[81,181],"cluster.":[82],"Using":[83],"targeted":[84],"mutagenesis,":[85],"we":[86],"identify":[87],"rcoM":[88],",":[89,91,93,104],"coxL":[90,130],"coxF":[92],"coxG":[95],"as":[96,187],"essential":[97],"for":[98,126,192],"oxidation,":[100],"whereas":[101],"coxD,":[102],"coxE":[103],"mocA":[106],"are":[107,169],"non\u2010essential.":[108],"Deletion":[109],"accessory":[112],"produced":[115],"phenotypes":[117],"compared":[118],"A.":[120],"OM5,":[122],"revealing":[123],"alternative":[124],"routes":[125],"maturation.":[128],"Both":[129],"paralogs":[131],"were":[132],"deleted":[133],"singly":[134],"double":[137],"knockouts,":[138],"providing":[139],"first":[141],"molecular":[142],"evidence":[143],"that":[144,164],"form\u2010II":[145],"does":[147],"not":[148,170],"contribute":[149],"vivo":[154],"these":[156,161],"groups":[157],"bacteria.":[159,203],"Together,":[160],"findings":[162],"show":[163],"function":[168],"universally":[171],"conserved":[172],"caution":[174],"against":[175],"inferring":[176],"CO\u2010oxidising":[177],"capacity":[178],"solely":[179],"from":[180],"presence.":[182],"Pb.":[183],"COX":[185],"emerges":[186],"genetically":[189],"tractable":[190],"model":[191],"elucidating":[193],"diversity":[195],"evolution":[197],"metalloenzyme":[199],"assembly":[200],"pathways":[201]},"counts_by_year":[],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2026-11-03T00:00:00"}
