{"id":"https://openalex.org/W7225081786","doi":"https://doi.org/10.64898/2026.10.30.763064","title":"MaCADEMIA: a software toolbox for single-molecule fluorescence image quantification","display_name":"MaCADEMIA: a software toolbox for single-molecule fluorescence image quantification","publication_year":2026,"publication_date":"2026-11-02","ids":{"openalex":"https://openalex.org/W7225081786","doi":"https://doi.org/10.64898/2026.10.30.763064"},"language":"en","primary_location":{"id":"doi:10.64898/2026.10.30.763064","doi":"https://doi.org/10.64898/2026.10.30.763064","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.30.763064","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/02/2026.10.30.763064.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/02/2026.10.30.763064.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5160332253","display_name":"Dominic Alderson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Dominic Alderson","raw_affiliation_strings":["Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","institution_ids":["https://openalex.org/I84884186"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5092866932","display_name":"Zoe Catchpole","orcid":"https://orcid.org/0009-0005-8206-4546","observed_orcids":["https://orcid.org/0009-0005-8206-4546"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zoe Catchpole","raw_affiliation_strings":["Translational and Clinical Research Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Translational and Clinical Research Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065529393","display_name":"Katrina E Crompton","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Katrina Crompton","raw_affiliation_strings":["Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","institution_ids":["https://openalex.org/I84884186"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160331979","display_name":"Katherine Sanders","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Katherine Sanders","raw_affiliation_strings":["Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","institution_ids":["https://openalex.org/I84884186"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160359966","display_name":"Sophia Johnson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Sophia Johnson","raw_affiliation_strings":["Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","institution_ids":["https://openalex.org/I84884186"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5137572081","display_name":"Kirsty Smith","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Kirsty Smith","raw_affiliation_strings":["Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","institution_ids":["https://openalex.org/I84884186"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5160369158","display_name":"John Packer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"John Packer","raw_affiliation_strings":["Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","institution_ids":["https://openalex.org/I84884186"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5114082298","display_name":"Kate S. Harris","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Kate S. Harris","raw_affiliation_strings":["School of Natural and Environmental Science, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","Translational and Clinical Research Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Natural and Environmental Science, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","institution_ids":["https://openalex.org/I84884186"]},{"raw_affiliation_string":"Translational and Clinical Research Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020024239","display_name":"Josana Rodr\u0131\u0301guez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Josana Rodriguez","raw_affiliation_strings":["Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","institution_ids":["https://openalex.org/I84884186"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074311677","display_name":"Mark C. Leake","orcid":"https://orcid.org/0000-0002-1715-1249","observed_orcids":["https://orcid.org/0000-0002-1715-1249"]},"institutions":[{"id":"https://openalex.org/I190082696","display_name":"Durham University","ror":"https://ror.org/01v29qb04","country_code":"GB","type":"education","lineage":["https://openalex.org/I190082696"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Mark C. Leake","raw_affiliation_strings":["Biophysical Sciences Institute, Durham University, Durham, DH1 3LE, United Kingdom","Departments of Biosciences and Physics, Durham University, Durham, DH1 3LE United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biophysical Sciences Institute, Durham University, Durham, DH1 3LE, United Kingdom","institution_ids":["https://openalex.org/I190082696"]},{"raw_affiliation_string":"Departments of Biosciences and Physics, Durham University, Durham, DH1 3LE United Kingdom","institution_ids":["https://openalex.org/I190082696"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037565193","display_name":"Adam J. M. Wollman","orcid":"https://orcid.org/0000-0002-5501-8131","observed_orcids":["https://orcid.org/0000-0002-5501-8131"]},"institutions":[{"id":"https://openalex.org/I84884186","display_name":"Newcastle University","ror":"https://ror.org/01kj2bm70","country_code":"GB","type":"education","lineage":["https://openalex.org/I84884186"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Adam J. M Wollman","raw_affiliation_strings":["Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom"],"raw_orcid":"https://orcid.org/0000-0002-5501-8131","affiliations":[{"raw_affiliation_string":"Biosciences Institute, Newcastle-upon-Tyne, NE2 4HH, United Kingdom","institution_ids":["https://openalex.org/I84884186"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5037565193"],"corresponding_institution_ids":["https://openalex.org/I84884186"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/single-molecule-localization-microscopy","display_name":"single-molecule localization microscopy","score":0.9879999756813049},{"id":"https://openalex.org/keywords/super-resolution-microscopy","display_name":"super-resolution microscopy","score":0.890999972820282},{"id":"https://openalex.org/keywords/quantitative-image-analysis","display_name":"quantitative image analysis","score":0.7620000243186951},{"id":"https://openalex.org/keywords/particle-detection","display_name":"particle detection","score":0.824999988079071},{"id":"https://openalex.org/keywords/particle-tracking","display_name":"particle tracking","score":0.6520000100135803},{"id":"https://openalex.org/keywords/copy-number-quantification","display_name":"copy number quantification","score":0.7730000019073486},{"id":"https://openalex.org/keywords/mean-square-displacement","display_name":"mean square displacement","score":0.9629999995231628},{"id":"https://openalex.org/keywords/matlab-toolbox","display_name":"MATLAB toolbox","score":0.515999972820282},{"id":"https://openalex.org/keywords/molecular-diffusion","display_name":"molecular diffusion","score":0.31200000643730164}],"concepts":[{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6474000215530396},{"id":"https://openalex.org/C2777655017","wikidata":"https://www.wikidata.org/wiki/Q1501161","display_name":"Toolbox","level":2,"score":0.6355999708175659},{"id":"https://openalex.org/C2777904410","wikidata":"https://www.wikidata.org/wiki/Q7397","display_name":"Software","level":2,"score":0.6154000163078308},{"id":"https://openalex.org/C160633673","wikidata":"https://www.wikidata.org/wiki/Q355198","display_name":"Pixel","level":2,"score":0.6114000082015991},{"id":"https://openalex.org/C139945424","wikidata":"https://www.wikidata.org/wiki/Q1940696","display_name":"Mean squared error","level":2,"score":0.4715999960899353},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.46459999680519104},{"id":"https://openalex.org/C2776401178","wikidata":"https://www.wikidata.org/wiki/Q12050496","display_name":"Feature (linguistics)","level":2,"score":0.4392000138759613},{"id":"https://openalex.org/C9417928","wikidata":"https://www.wikidata.org/wiki/Q1070689","display_name":"Image processing","level":3,"score":0.4262999892234802},{"id":"https://openalex.org/C153180895","wikidata":"https://www.wikidata.org/wiki/Q7148389","display_name":"Pattern recognition (psychology)","level":2,"score":0.40720000863075256},{"id":"https://openalex.org/C205372480","wikidata":"https://www.wikidata.org/wiki/Q210521","display_name":"Image resolution","level":2,"score":0.3831000030040741},{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.37940001487731934},{"id":"https://openalex.org/C2775936607","wikidata":"https://www.wikidata.org/wiki/Q466845","display_name":"Tracking (education)","level":2,"score":0.37439998984336853},{"id":"https://openalex.org/C52622490","wikidata":"https://www.wikidata.org/wiki/Q1026626","display_name":"Feature extraction","level":2,"score":0.357699990272522},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.35280001163482666},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.351500004529953},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.3409999907016754},{"id":"https://openalex.org/C165064840","wikidata":"https://www.wikidata.org/wiki/Q1321061","display_name":"Matching (statistics)","level":2,"score":0.3384000062942505},{"id":"https://openalex.org/C2780365114","wikidata":"https://www.wikidata.org/wiki/Q169478","display_name":"MATLAB","level":2,"score":0.33070001006126404},{"id":"https://openalex.org/C31332276","wikidata":"https://www.wikidata.org/wiki/Q899750","display_name":"Photobleaching","level":3,"score":0.3260999917984009},{"id":"https://openalex.org/C53533937","wikidata":"https://www.wikidata.org/wiki/Q185020","display_name":"Histogram","level":3,"score":0.31929999589920044},{"id":"https://openalex.org/C115961682","wikidata":"https://www.wikidata.org/wiki/Q860623","display_name":"Image (mathematics)","level":2,"score":0.3109999895095825},{"id":"https://openalex.org/C4924752","wikidata":"https://www.wikidata.org/wiki/Q184148","display_name":"Plug-in","level":2,"score":0.2728999853134155},{"id":"https://openalex.org/C52421305","wikidata":"https://www.wikidata.org/wiki/Q1151499","display_name":"Particle filter","level":3,"score":0.25029999017715454}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.64898/2026.10.30.763064","doi":"https://doi.org/10.64898/2026.10.30.763064","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.30.763064","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/02/2026.10.30.763064.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.64898/2026.10.30.763064","doi":"https://doi.org/10.64898/2026.10.30.763064","is_oa":true,"landing_page_url":"https://doi.org/10.64898/2026.10.30.763064","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2026/11/02/2026.10.30.763064.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1522166032","display_name":"Understanding how PAR proteins cooperate to establish cell polarity (New Investigator)","funder_award_id":"BB/R019436/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"},{"id":"https://openalex.org/G2438624709","display_name":"The Biophysics of Mesoscale, Reversible, Biomolecular Assemblies","funder_award_id":"EP/Y000501/1","funder_id":"https://openalex.org/F4320334627","funder_display_name":"Engineering and Physical Sciences Research Council"}],"funders":[{"id":"https://openalex.org/F4320320006","display_name":"Royal Society","ror":"https://ror.org/03wnrjx87"},{"id":"https://openalex.org/F4320334627","display_name":"Engineering and Physical Sciences Research Council","ror":"https://ror.org/0439y7842"},{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W7225081786.pdf","grobid_xml":"https://content.openalex.org/works/W7225081786.grobid-xml"},"referenced_works_count":2,"referenced_works":["https://openalex.org/W2950722221","https://openalex.org/W4388179024"],"related_works":["https://openalex.org/W2953330751","https://openalex.org/W3004731352","https://openalex.org/W7095768518","https://openalex.org/W2911956729","https://openalex.org/W4387263822","https://openalex.org/W4210409543","https://openalex.org/W2789774265","https://openalex.org/W7021093501","https://openalex.org/W3075227687","https://openalex.org/W1990917547"],"abstract_inverted_index":{"Abstract":[0],"Single-molecule":[1],"localisation":[2,111],"microscopy":[3],"(SMLM)":[4],"is":[5,28,52],"a":[6,86],"powerful":[7,89],"tool":[8],"in":[9,46,60],"molecular":[10,42,146],"and":[11,14,44,84,94,148,163],"cellular":[12,171],"biology":[13],"part":[15],"of":[16,20,26,79,91,101,138],"the":[17,61,136],"broad":[18],"umbrella":[19],"super-resolution":[21],"microscopy.":[22],"A":[23],"distinct":[24],"feature":[25],"SMLM,":[27],"its":[29],"capacity":[30],"for":[31],"deep":[32],"quantification,":[33],"beyond":[34],"just":[35],"spatial":[36],"information,":[37],"to":[38,55,97,114],"copy":[39,166],"number,":[40],"stoichiometry,":[41],"interaction":[43],"dynamics":[45],"living":[47],"cells.":[48],"The":[49],"hardware":[50],"required":[51],"increasingly":[53],"straightforward":[54,99],"implement.":[56],"However,":[57],"complexity":[58],"remains":[59,66],"image":[62],"analysis":[63,162],"where":[64],"much":[65],"fragmented":[67],"across":[68,122],"specialised":[69],"software":[70],"tools.":[71],"Here,":[72],"we":[73],"present":[74],"MaCADEMIA":[75,105],"(Matlab":[76],"Computational":[77],"Analysis":[78],"Diffusion,":[80],"Emitter":[81],"Molecular":[82],"Intensity":[83],"Abundance),":[85],"simple":[87],"yet":[88],"toolbox":[90],"MATLAB":[92],"functions":[93],"associated":[95],"documentation":[96],"enable":[98],"extraction":[100],"quantitative":[102],"single-molecule":[103],"information.":[104],"performs":[106],"ensemble":[107],"particle":[108,129],"detection":[109],"achieving":[110],"accuracies":[112],"down":[113],"0.5":[115],"pixels":[116],"Root":[117],"mean":[118,158],"squared":[119,159],"error":[120],"(RMSE)":[121],"wide":[123],"signal-to-noise":[124],"(SNR)":[125],"ranges":[126],"on":[127],"standardised":[128],"tracking":[130],"benchmarks,":[131],"consistently":[132],"matching":[133],"or":[134],"exceeding":[135],"performance":[137],"14":[139],"leading":[140],"methods.":[141],"Our":[142],"integrated":[143],"workflow":[144],"enables":[145],"counting":[147],"stoichiometry":[149],"determination":[150],"through":[151],"stepwise":[152],"photobleaching":[153],"analysis,":[154],"mobility":[155],"measurement":[156],"via":[157],"displacement,":[160],"colocalisaton":[161],"absolute":[164],"protein":[165],"number":[167],"quantification":[168],"using":[169],"three-dimensional":[170],"models.":[172]},"counts_by_year":[],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2026-11-03T00:00:00"}